Doublecortin Antibody


Western Blot: Doublecortin Antibody [NBP1-72042] - (0.01ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody [NBP1-72042] - analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 more
Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP1-72042] - Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
Western Blot: Doublecortin Antibody [NBP1-72042] - (0.01ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP1-72042] - (2ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

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Doublecortin Antibody Summary

Peptide with sequence C-KTSANMKAPQS corresponding to internal region according to NP_835365.1, NP_835364.1, NP_001182482.1.
Immature Neuronal Marker
This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
Immunogen affinity purified
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  • Western Blot 0.01 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
Western blot: Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3kDa according to Human NP_000546.2 and 40.6kDa according to Human NP_835365.1 and Mouse NP_001103692.1). Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain for this particular batch.
IHC: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells.
Read Publication using NBP1-72042.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for Doublecortin Antibody

  • doublecortin
  • Doublin
  • FLJ51296
  • Lissencephalin-X
  • Lis-X
  • X-linked (doublecortin)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Doublecortin Antibody (NBP1-72042)(1)

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Video Protocols

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ICC/IF Video Protocol

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Gene Symbol DCX