Doublecortin Antibody

Images

 
Western Blot: Doublecortin Antibody [NBP1-72042] - staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed ...read more
Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP1-72042] - Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed ...read more
Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP1-72042] - Staining of paraffin embedded Human Cerebellum. Antibody at 2 ug/mL. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Doublecortin Antibody Summary

Immunogen
Peptide with sequence C-KTSANMKAPQS corresponding to internal region according to NP_835365.1, NP_835364.1, NP_001182482.1.
Marker
Immature Neuronal Marker
Specificity
This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/mL
  • Immunocytochemistry/Immunofluorescence 5 - 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4 ug/mL
  • Peptide ELISA Detection limit 1:32000
Application Notes
Western blot: Approx 45 kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3 kDa according to Mouse NP_001103692.1).
IHC: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and KNRK cells.
Publications
Read Publications using NBP1-72042.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Doublecortin Antibody

  • DBCN
  • Doublecortex
  • Doublecortin
  • Doublin
  • FLJ51296
  • Lissencephalin-X
  • LISX
  • Lis-X
  • X-linked (doublecortin)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Doublecortin Antibody (NBP1-72042)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DCX
Entrez
Uniprot