CD28 Antibody (37407) [Unconjugated] (MAB342)

Images

 
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, AgAct
Clone
37407
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

CD28 Antibody (37407) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD28
Asn19-Pro152
Accession # P10747
Specificity
Detects human CD28 in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) CD28, rhCTLA4, or rmCTLA4.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD28
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Agonist Activity 0.2-0.6 ug/mL
Publications
Read Publications using
MAB342 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD28 Antibody (37407) [Unconjugated]

  • CD28 antigen (Tp44)
  • CD28 antigen
  • CD28 molecule
  • CD28
  • MGC138290
  • T-cell-specific surface glycoprotein CD28
  • Tp44

Background

CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up-regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the upregulation of Bcl-xL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB342
Species: Hu
Applications: WB, AgAct

Publications for CD28 Antibody (MAB342)(22)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: Binding Assay, Bioassay, ELISA Development, Functional Assay, ICC, Neut, WB.


Filter By Application
Binding Assay
(1)
Bioassay
(3)
ELISA Development
(1)
Functional Assay
(13)
ICC
(1)
Neut
(1)
WB
(1)
All Applications
Filter By Species
Human
(21)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using MAB342 Applications Species
C Tao, H Shao, W Zhang, H Bo, F Wu, H Shen, S Huang ??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function Mol Med Rep, 2017;0(0):. 2017 [PMID: 28259946] (Bioassay,, Human) Bioassay Human
Van Belle T, Vanherwegen A, Feyaerts D, De Clercq P, Verstuyf A, Korf H, Gysemans C, Mathieu C 1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients. PLoS ONE, 2014;9(10):e109194. 2014 [PMID: 25279717]
Zhu L, Huang Z, Stalesen R, Hansson G, Li N Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation. J Thromb Haemost, 2014;12(7):1156-65. 2014 [PMID: 24833264] (Bioassay,, Human) Bioassay Human
Foley M, Forcier T, McAndrew E, Gonzalez M, Chen H, Juelg B, Walker B, Irvine D High avidity CD8+ T cells efficiently eliminate motile HIV-infected targets and execute a locally focused program of anti-viral function. PLoS ONE, 2014;9(2):e87873. 2014 [PMID: 24551068] (Functional Assay,, Human) Functional Assay Human
Dalmas E, Venteclef N, Caer C, Poitou C, Cremer I, Aron-Wisnewsky J, Lacroix-Desmazes S, Bayry J, Kaveri S, Clement K, Andre S, Guerre-Millo M T cell-derived IL-22 amplifies IL-1beta-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes. Diabetes, 2014;63(6):1966-77. 2014 [PMID: 24520123] (Neut,, Human) Neut Human
Renand A, Milpied P, Rossignol J, Bruneau J, Lemonnier F, Dussiot M, Coulon S, Hermine O Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs. PLoS ONE, 2013;8(12):e85589. 2013 [PMID: 24386482] (Functional Assay,, Human) Functional Assay Human
Gironi, Maira, Saresella, Marina, Rovaris, Marco, Vaghi, Matilde, Nemni, Raffaell, Clerici, Mario, Grossi, Enzo A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis. Immun Ageing, 2013;10(1):1. 2013 [PMID: 23305498] (Bioassay,, Human) Bioassay Human
Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis. Eur. J. Immunol., 2009;39(12):3570-81. 2009 [PMID: 19830738] (Functional Assay,, Human) Functional Assay Human
Hirosako S, Goto E, Fujii K, Tsumori K, Hirata N, Tsumura S, Kamohara H, Kohrogi H Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells. Clin. Exp. Immunol., 2009;155(2):266-74. 2009 [PMID: 19040600] (Functional Assay,, Human) Functional Assay Human
Lazer G, Pe'er L, Schapira V, Richard S, Katzav S The association of Sam68 with Vav1 contributes to tumorigenesis. Cell. Signal., 2007;19(12):2479-86. 2007 [PMID: 17855053] (ICC,, Human) ICC Human
Show All 22 Publications.

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Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Bioinformatics

Gene Symbol CD28
Entrez
Uniprot

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