CD28 Antibody (37407) [Unconjugated]


Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent more
CD28 was detected in immersion fixed U266 human myeloma cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative) using Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342) at more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Func, ICC/IF

CD28 Antibody (37407) [Unconjugated] Summary

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD28
Accession # P10747
Detects human CD28 in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) CD28, rhCTLA4, or rmCTLA4.
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Agonist Activity 0.2-0.6 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
MAB342 in the following applications:

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MAB342 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD28 Antibody (37407) [Unconjugated]

  • CD28 antigen (Tp44)
  • CD28 antigen
  • CD28 molecule
  • CD28
  • MGC138290
  • T-cell-specific surface glycoprotein CD28
  • Tp44


CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up-regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the upregulation of Bcl-xL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.

  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD28 Antibody (MAB342)(40)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 13 applications: Binding Assay, Bioassay, Cell Culture, ELISA Development, Flow Cytometry, Functional Assay, ICC, Neutralization, Polarization, Stimulation, T Cell Activation, Tissue Culture, Western Blot.

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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using MAB342 Applications Species
Popugailo, A;Rotfogel, Z;Levy, M;Turgeman, O;Hillman, D;Levy, R;Arad, G;Shpilka, T;Kaempfer, R; The homodimer interfaces of costimulatory receptors B7 and CD28 control their engagement and pro-inflammatory signaling Journal of biomedical science 2023-06-28 [PMID: 37381064] (Cell Culture, Human) Cell Culture Human
Mez?si-Csaplár, M;Szö?r, Á;Vereb, G; CD28 and 41BB Costimulatory Domains Alone or in Combination Differentially Influence Cell Surface Dynamics and Organization of Chimeric Antigen Receptors and Early Activation of CAR T Cells Cancers 2023-06-07 [PMID: 37370693] (Bioassay, Human) Bioassay Human
A Lanna, B Vaz, C D'Ambra, S Valvo, C Vuotto, V Chiurchiù, O Devine, M Sanchez, G Borsellino, AN Akbar, M De Bardi, DW Gilroy, ML Dustin, B Blumer, M Karin An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory Nature Cell Biology, 2022-09-15;0(0):. 2022-09-15 [PMID: 36109671] (Flow Cytometry, Human) Flow Cytometry Human
L Yang, S Liu, Q Zhang, S Jia, C Qiu, Z Jin Overexpression of ascitic interleukin-35 induces CD8+ T cell exhaustion in liver cirrhotic patients with spontaneous bacterial peritonitis International immunopharmacology, 2022-03-26;108(0):108729. 2022-03-26 [PMID: 35349961] (Bioassay, Human) Bioassay Human
Guo J, Luo N, Ai G, Yang W, Zhu J, Li C, Chen R, Zhang C, Liu S, Jin H, Cheng Z Eradicating tumor in a recurrent cervical cancer patient with autologous tumor-infiltrating lymphocytes and a modified lymphodepleting regimen. J Immunother Cancer, 2022-02-01;10(2):. 2022-02-01 [PMID: 35177415] (Functional Assay, Human) Functional Assay Human
MG Jeong, K Zhou, S Park, H An, Y Kwon, Y Chang, DH Kim, SH Ryu Analysis of transient membrane protein interactions by single-molecule diffusional mobility shift assay Experimental & Molecular Medicine, 2021-02-19;0(0):. 2021-02-19 [PMID: 33603128] (ICC, Human) ICC Human
X Zhuang, F Maione, J Robinson, M Bentley, B Kaul, K Whitworth, N Jumbu, E Jinks, J Bystrom, P Gabriele, E Garibaldi, E Delmastro, Z Nagy, D Gilham, E Giraudo, R Bicknell, SP Lee CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth JCI Insight, 2020-10-02;5(19):. 2020-10-02 [PMID: 33004686] (Bioassay, Human) Bioassay Human
J Huetter, U Gritzan, I Gutcher, WD Doecke, MV Luetke-Eve, S Golfier, HG Roider, AL Frisk, J Hunter, A Pow, A Drake, Z Levine, O Levy, M Azulay, I Barbiro, G Cojocaru, I Vaknin, B Kreft, L Roese Characterization of BAY 1905254, an Immune Checkpoint Inhibitor Targeting the Immunoglobulin-Like Domain Containing Receptor 2 (ILDR2) Cancer Immunol Res, 2020-04-20;8(7):895-911. 2020-04-20 [PMID: 32312711] (Functional Assay, T Cell Activation, Human) Functional Assay, T Cell Activation Human
Z Hu, P Yu, G Du, W Wang, H Zhu, N Li, H Zhao, Z Dong, L Ye, J Tian PCC0208025 (BMS202), a small molecule inhibitor of PD-L1, produces an antitumor effect in B16-F10 melanoma-bearing mice PLoS ONE, 2020-03-26;15(3):e0228339. 2020-03-26 [PMID: 32214351] (Flow Cytometry, Mouse) Flow Cytometry Mouse
ML Tiirikaine, A Woetmann, H Norsgaard, LF Santamaria, P Lovato Ex vivo culture of lesional psoriasis skin for pharmacological testing J. Dermatol. Sci., 2019-12-27;0(0):. 2019-12-27 [PMID: 31948839] (Tissue Culture, Human) Tissue Culture Human
Show All 40 Publications.

Review for CD28 Antibody (MAB342) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB342:
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Images Ratings Applications Species Date Details
Western Blot CD28 MAB342
reviewed by:
Verified Customer
WB Human 02/10/2022


ApplicationWestern Blot
Sample TestedT cells

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Verified Customer
Application: WB
Species: Human


Gene Symbol CD28