CD28 Antibody (37407) [Unconjugated]


Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent more

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Reactivity HuSpecies Glossary
Applications WB, Func

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CD28 Antibody (37407) [Unconjugated] Summary

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD28
Accession # P10747
Detects human CD28 in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) CD28, rhCTLA4, or rmCTLA4.
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
  • Agonist Activity 0.2-0.6 ug/mL
CD28 Knockout HeLa Cell Lysate
Read Publications using
MAB342 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD28 Antibody (37407) [Unconjugated]

  • CD28 antigen (Tp44)
  • CD28 antigen
  • CD28 molecule
  • CD28
  • MGC138290
  • T-cell-specific surface glycoprotein CD28
  • Tp44


CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up-regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the upregulation of Bcl-xL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.

  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD28 Antibody (MAB342)(30)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 10 applications: Binding Assay, Bioassay, ELISA Development, Flow Cytometry, Functional Assay, ICC, Neutralization, Stimulation, Tissue Culture, Western Blot.

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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using MAB342 Applications Species
ML Tiirikaine, A Woetmann, H Norsgaard, LF Santamaria, P Lovato Ex vivo culture of lesional psoriasis skin for pharmacological testing J. Dermatol. Sci., 2019;0(0):. 2019 [PMID: 31948839] (Tissue Culture, Human) Tissue Culture Human
Y Lu, BK Yoo, AHC Ng, J Kim, S Yeom, J Tang, MM Lin, AH Zewail, JR Heath 4D electron microscopy of T cell activation Proc. Natl. Acad. Sci. U.S.A., 2019;116(44):22014-22019. 2019 [PMID: 31611413] (Functional Assay, Human) Functional Assay Human
JC Chamcheu, S Esnault, VM Adhami, AL Noll, S Banang-Mbe, T Roy, SS Singh, S Huang, KG Kousoulas, H Mukhtar Fisetin, a 3,7,3&#039;,4&#039;-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models Cells, 2019;8(9):. 2019 [PMID: 31540162] (Functional Assay, Human) Functional Assay Human
NB Zhou, KG Wang, ZJ Fu Effect of morphine and a low dose of ketamine on the T cells of patients with refractory cancer pain in vitro Oncol Lett, 2019;18(4):4230-4236. 2019 [PMID: 31516618] (Stimulation, Human) Stimulation Human
F Bensch, EL van der Ve, MN Lub-de Hoo, A Jorritsma-, R Boellaard, IC Kok, SF Oosting, CP Schröder, TJN Hiltermann, AJ van der We, HJM Groen, TC Kwee, SG Elias, JA Gietema, SS Bohorquez, A de Crespig, SP Williams, C Mancao, AH Brouwers, BM Fine, EGE de Vries 89Zr-atezolizumab imaging as a non-invasive approach to assess clinical response to PD-L1 blockade in cancer Nat. Med., 2018;0(0):. 2018 [PMID: 30478423] (Bioassay, Human) Bioassay Human
V Giacomet, M Masetti, P Nannini, F Forlanini, M Clerici, GV Zuccotti, D Trabattoni Humoral and cell-mediated immune responses after a booster dose of HBV vaccine in HIV-infected children, adolescents and young adults PLoS ONE, 2018;13(2):e0192638. 2018 [PMID: 29444185] (Functional Assay, Human) Functional Assay Human
C Caër, C Rouault, T Le Roy, C Poitou, J Aron-Wisne, A Torcivia, JC Bichet, K Clément, M Guerre-Mil, S André Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue Sci Rep, 2017;7(1):3000. 2017 [PMID: 28592801] (Flow Cytometry, Human) Flow Cytometry Human
S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis Arthritis Res. Ther., 2017;19(1):97. 2017 [PMID: 28511719] (Functional Assay, Human) Functional Assay Human
C Tao, H Shao, W Zhang, H Bo, F Wu, H Shen, S Huang ??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function Mol Med Rep, 2017;0(0):. 2017 [PMID: 28259946] (Bioassay, Human) Bioassay Human
Van Belle T, Vanherwegen A, Feyaerts D, De Clercq P, Verstuyf A, Korf H, Gysemans C, Mathieu C 1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients. PLoS ONE, 2014;9(10):e109194. 2014 [PMID: 25279717]
Show All 30 Publications.

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Gene Symbol CD28