beta-Actin Antibody (8H10D10) [DyLight 350]

Images

 
There are currently no images for beta-Actin Antibody (NBP1-47423UV).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, PmSpecies Glossary
Applications WB, ELISA, Flow, IB, ICC/IF
Clone
8H10D10
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

Order Details

beta-Actin Antibody (8H10D10) [DyLight 350] Summary

Immunogen
This beta-Actin Antibody (8H10D10) was made to a synthetic peptide corresponding to amino-terminal residues of human Beta Actin, conjugated to KLH. [UniProt# P60709]
Localization
Cytoplasm, cytoskeleton.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ACTB
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Ammonium sulfate precipitation

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for beta-Actin Antibody (8H10D10) [DyLight 350]

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • B-Actin
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • I(2)-Actin
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single-Cell Western
NBP1-33527
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB500-317
Species: Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA
NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, KD, KO
NBP2-59208
Species: Hu, Mu
Applications: WB, ChIP, CHIP-SEQ, KD
NBP2-24571
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P
NBP1-32710
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF3589
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
NBP2-67130
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P
MAB2669
Species: Hu
Applications: WB, Simple Western, IHC
AF6144
Species: Hu
Applications: WB, Simple Western
NBP1-87329
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-19784
Species: Hu, Mu, Rt, Ye
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, PLA
NBP1-82859
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB300-130
Species: Hu, Mu, Rt, Po, Bv, Ce, Ch, Dr, Eq, Pm, Pl, Ze
Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-94633
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
H00006124-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
NBP2-75478
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-88192
Species: Hu
Applications: WB, IHC, IHC-P

Publications for beta-Actin Antibody (NBP1-47423UV) (0)

There are no publications for beta-Actin Antibody (NBP1-47423UV).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for beta-Actin Antibody (NBP1-47423UV) (0)

There are no reviews for beta-Actin Antibody (NBP1-47423UV). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NBP1-47423UV). (Showing 1 - 5 of 5 FAQ).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What is the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW for beta Actin antibodies is approximately 42 kDa.
  5. I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
    • For homogenized tissue, beta-actin and GAPDH are both fine.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-47423AF350
Alexa Fluor 405 NBP1-47423AF405
Alexa Fluor 488 NBP1-47423AF488
Alexa Fluor 532 NBP1-47423AF532
Alexa Fluor 594 NBP1-47423AF594
Alexa Fluor 647 NBP1-47423AF647
Alexa Fluor 700 NBP1-47423AF700
Alexa Fluor 750 NBP1-47423AF750
Allophycocyanin NBP1-47423APC
Allophycocyanin/Cy7 NBP1-47423APCCY7
Biotin NBP1-47423B
DyLight 350 NBP1-47423UV
DyLight 405 NBP1-47423V
DyLight 488 NBP1-47423G
DyLight 550 NBP1-47423R
DyLight 594 NBP1-47423DL594
DyLight 650 NBP1-47423C
DyLight 680 NBP1-47423FR
DyLight 755 NBP1-47423IR
FITC NBP1-47423F
HRP NBP1-47423H
Janelia Fluor 549 NBP1-47423JF549
Janelia Fluor 646 NBP1-47423JF646
PE NBP1-47423PE
PE/Atto594 NBP1-47423PEATT594
PE/Cy5.5 NBP1-47423PECY55
PE/Cy7 NBP1-47423PECY7
PerCP NBP1-47423PCP

Additional beta-Actin Products

Bioinformatics Tool for beta-Actin Antibody (NBP1-47423UV)

Discover related pathways, diseases and genes to beta-Actin Antibody (NBP1-47423UV). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for beta-Actin Antibody (NBP1-47423UV)

Discover more about diseases related to beta-Actin Antibody (NBP1-47423UV).
 

Pathways for beta-Actin Antibody (NBP1-47423UV)

View related products by pathway.

PTMs for beta-Actin Antibody (NBP1-47423UV)

Learn more about PTMs related to beta-Actin Antibody (NBP1-47423UV).
 

Research Areas for beta-Actin Antibody (NBP1-47423UV)

Find related products by research area.

Blogs on beta-Actin.


  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our beta-Actin Antibody (8H10D10) [DyLight 350] and receive a gift card or discount.

Bioinformatics

Gene Symbol ACTB