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beta-Actin Antibody (8H10D10) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
8H10D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-47423AF405
Alexa Fluor 647
NBP1-47423AF647
Alexa Fluor 700
NBP1-47423AF700
Allophycocyanin
NBP1-47423APC
Biotin
NBP1-47423B
DyLight 350
NBP1-47423UV
DyLight 405
NBP1-47423V
DyLight 488
NBP1-47423G
DyLight 550
NBP1-47423R
DyLight 650
NBP1-47423C
DyLight 680
NBP1-47423FR
DyLight 755
NBP1-47423IR
FITC
NBP1-47423F
HRP
NBP1-47423H
Janelia Fluor 549
NBP1-47423JF549
Janelia Fluor 646
NBP1-47423JF646
PE
NBP1-47423PE
PerCP
NBP1-47423PCP
Unconjugated
NBP1-47423
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beta-Actin Antibody (8H10D10) [Alexa Fluor® 488] Summary

Immunogen
Synthetic peptide corresponding to amino-terminal residues of human Beta Actin, conjugated to KLH. [UniProt# P60709]
Localization
Cytoplasm, cytoskeleton.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ACTB
Purity
Ammonium sulfate precipitation
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human, mouse, rat, Chinese hamster and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Ammonium sulfate precipitation

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for beta-Actin Antibody (8H10D10) [Alexa Fluor® 488]

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Image from verified customer review.
Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH, NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - SH-SY5Y cells stained with GAPDH antibody NB300-221 (green) and Neurofilament Heavy antibody NB300-217 (red), counterstained with a fluorescent DNA probe (blue).
(16 Reviews)
(254 Publications)
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Eq, Fe, Ha, Op, Pm, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP
HPRT Antibody
NBP1-33527
Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded rat colon. HPRT antibody diluted at 1:500.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
beta
2-Microglobulin Antib
NB500-317
Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.
Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Confocal microscopy of human beta2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.
Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PE.
(2 Publications)
Species: Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA
beta-Actin
Antibody (AC-15)
NB600-501
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Simple Western lane view shows a specific band for Beta-Actin in 0.2 mg/ml of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
(22 Reviews)
(314 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
TATA binding
protein TBP Antibody
NBP2-12933
Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Staining of Pig Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - NBP2-12933 (1ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Analysis of cell line NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Species: Hu, Mu, Rt, Po
Applications: WB, PEP-ELISA
EIF3B Antibody
NBP2-24571
Western Blot: EIF3B Antibody [NBP2-24571] - analysis of eIF3B in A431 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using eIF3B antibody at 0.25 ug/ml, 0.25 ug/ml and 0.05 ug/ml respectively.
Immunohistochemistry-Paraffin: EIF3B Antibody [NBP2-24571] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Simple Western: EIF3B Antibody [NBP2-24571] - Simple Western lane view shows a specific band for EIF3B in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Species: Hu, Mu
Applications: WB, Simple Western, IHC-P
RPL13A Antibody
NBP1-92345
Western Blot: RPL13A Antibody [NBP1-92345] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: RPL13A Antibody [NBP1-92345] - Staining of human cell line U-2 OS shows localization to nucleoli & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPL13A Antibody [NBP1-92345] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Cyclophilin A
Antibody (1F4-1B5)
H00005478-M01
Western Blot: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01] - PPIA monoclonal antibody (M01), clone 1F4-1B5 Analysis of PPIA expression in Jurkat.
Western Blot: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01] - Analysis of PPIA expression in transfected 293T cell line by PPIA monoclonal antibody (M01), clone 1F4-1B5.Lane 1: PPIA transfected lysate(18 KDa).Lane 2: Non-transfected lysate.
ELISA: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01] - Detection limit for recombinant GST tagged PPIA is approximately 0.03ng/ml as a capture antibody.
(4 Publications)
Species: Hu
Applications: WB, ELISA
SDHA Antibody
NBP1-71688
Western Blot: SDHA Antibody [NBP1-71688] - Analysis of SDHA in NIH/3T3 cells.
Immunocytochemistry/Immunofluorescence: SDHA Antibody [NBP1-71688] - SDHA antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).
Western Blot: SDHA Antibody [NBP1-71688] - analysis of SDHA in whole skeletal muscle lysate or purified mitochondria from pig, mouse, lamb and goat using anti-SDHA antibody. Image from verified customer review.
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14-3-3 zeta
Antibody (818515) [Un ...
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Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line, loaded at 0.5 mg/mL. A specific band was detected for 14‑3‑3 zeta at approximately 34 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
14‑3‑3 zeta  was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to nuclei and plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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beta-Glucuronidase/GUS
6144-GH
beta-Glucuronidase/GUSB [Unconjugated]
Species: Hu
HMBS Antibody
NBP2-33600
Western Blot: HMBS Antibody [NBP2-33600] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Immunocytochemistry/Immunofluorescence: HMBS Antibody [NBP2-33600] - Staining of human cell line A-431 shows localization to lipid droplets.
Immunohistochemistry-Paraffin: HMBS Antibody [NBP2-33600] - Staining in human bone marrow and cerebral cortex tissues using anti-HMBS antibody. Corresponding HMBS RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
PGK1 Antibody
NBP2-19784
Western Blot: PGK1 Antibody [NBP2-19784] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted by 1:1000.
Immunocytochemistry/Immunofluorescence: PGK1 Antibody [NBP2-19784] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGK1 protein stained by PGK1 antibody diluted at 1:20. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt, Ye
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
RPLP0 Antibody
NBP1-82859
Immunocytochemistry/Immunofluorescence: RPLP0 Antibody [NBP1-82859] - Staining of human cell line A-431 shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPLP0 Antibody [NBP1-82859] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RPLP0 Antibody [NBP1-82859] - Staining of human liver shows no positivity in hepatocytes as expected.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Ubiquitin Antibody
(Ubi-1)
NB300-130
Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Western blot of Ubiquitin expression on (Lane 1) poly-ubiquitin (lys63 linked), (Lane 2) pure ubiquitin and crude homogenates of adult rat, (Lane 3) cortex, (Lane 4) cerebellum and (Lane 5) brain stem.
Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - IHC-P staining of cerebral cortex tissue from an Alzheimer patient brain using Ubiquitin antibody (clone Ubi-1) at 1:1000 dilution. Neurofibrillary tangles and dystrophic neurites associated with senile plaques stain strongly with this antibody. In the center is a typical neurofibrillary tangle containing neuron.
Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - Staining of Ubiquitin in hippocampal tissue from an Alzheimer patient.
(3 Reviews)
(18 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ce, Ch, Dr, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Ubiquitin
U-100H
Ubiquitin
(1 Review)
(55 Publications)
Species: Hu
Ubiquitin C
Antibody
NBP2-54692
Western Blot: Ubiquitin C Antibody [NBP2-54692] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG Lane 4: Human plasma Lane 5: Human Liver tissue Lane 6: Human Tonsil tissue
Immunohistochemistry-Paraffin: Ubiquitin C Antibody [NBP2-54692] - Staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
Species: Hu
Applications: WB, IHC, IHC-P
RPL4 Antibody
NBP1-81329
Western Blot: RPL4 Antibody [NBP1-81329] - Crude ribosomes isolated from HepG2 cells. 10% SDS-PAGE. Blot was blocked in 5% milk 1 hr at RT and then overnight at 4C with anti-RPL4 antibody at 1:250. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: RPL4 Antibody [NBP1-81329] - Staining of human cell line A-431 shows localization to nuclear bodies, cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPL4 Antibody [NBP1-81329] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine pancreas.
(1 Review)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
eEF1A1 Antibody
(JB44-13)
NBP2-75478
Western Blot: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution.Positive control:Lane 1: Rat brain tissueLane 2: Mouse skeletal muscleLane 3: Mouse cerebellumLane 4: Rat skin Lane 5: Daudi
Immunocytochemistry/Immunofluorescence: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Staining eEF1A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: eEF1A1 Antibody (JB44-13) [NBP2-75478] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
RPS9 Antibody
NBP2-22298
Western Blot: RPS9 Antibody [NBP2-22298] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22298 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: RPS9 Antibody [NBP2-22298] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RPS9 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Species: Hu, Mu
Applications: IP (-), WB, IHC, IHC-P

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Product General Protocols

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WB Video Protocol
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FAQs for beta-Actin Antibody (NBP1-47423AF488). (Showing 1 - 4 of 4 FAQ).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.

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Blogs on beta-Actin.

Check out the latest blog posts on beta-Actin.
The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.
Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.
Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.
ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.
A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Bioinformatics

Gene Symbol ACTB
Entrez