beta-Actin Antibody (8H10D10) [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt, ChHa, Pm
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 8H10D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

beta-Actin Antibody (8H10D10) [Alexa Fluor® 488] Summary

• Immunogen: Synthetic peptide corresponding to amino-terminal residues of human Beta Actin, conjugated to KLH. [UniProt# P60709]
• Localization: Cytoplasm, cytoskeleton.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTB
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Ammonium sulfate precipitation

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for beta-Actin Antibody (8H10D10) [Alexa Fluor® 488]

• ACTB
• actin, beta
• actin, cytoplasmic 1
• Beta Actin
• beta cytoskeletal actin
• betaActin
• beta-Actin
• BRWS1
• PS1TP5-binding protein 1
• PS1TP5BP1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for beta-Actin Antibody (NBP1-47423AF488). (Showing 1 - 4 of 4 FAQ).

1. Why is beta actin used as a loading control in blotting ?
   • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
2. Are the beta-Actin antibodies validated in Simple Western?
   • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
3. Do Beta-Actin antibodies come in lyophilized format?
   • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
4. What the theoretical molecular weight for Beta-Actin antibodies?
   • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.

Bioinformatics

• Gene Symbol: ACTB