TLR2 Antibody (TL2.1)

Images

 
Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 ...read more
Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Expression of TLR2 was determined by western blotting. The immunoreactivity of the anti-TLR2 was confirmed with human intestinal lysate. Data are representative for ...read more
Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Monitoring of TLR2 expression in normal B cells and primary MCL cells cultured for 24h in the absence/presence of CD40L, IL-4, and TLR ligands. Filled curves refer ...read more
Flow (Cell Surface): TLR2 Antibody (TL2.1) [NB100-56722] - Flow (Cell Surface): - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on stable transfected cell line using this ...read more
Block/Neutralize: TLR2 Antibody (TL2.1) [NB100-56722] - Human monocyte-derived DCs were pre-incubated wit TLR-neutralizing antibodies including TLR2 antibody (NB100-56722) for 1 h. Afterwards, DCs were infected with H. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, RbSpecies Glossary
Applications WB, DB, ELISA, Flow, Flow-CS, Func, ICC/IF, In vitro, IP, B/N
Clone
TL2.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TLR2 Antibody (TL2.1) Summary

Immunogen
This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (Flo et al, 2000). The hybridoma supernatants were selected by flow cytometry.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TLR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Dot Blot
  • ELISA
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 2-5 ug
  • In vitro assay
  • Block/Neutralize
  • Flow (Cell Surface)
Application Notes
Use in Functional and Blocking / neutralizing reported in scientific literature (PMID 25412776). Use in Flow cell surface reported in scientific literature (PMID 24836676). Use in functional reported in scientific literature (PMID: 25412776). Use in Dot blot reported in scientific literature (PMID: 27248820). Use in In vitro reported in scientific literature (PMID: 24512642). Use in ELISA reported in multiple pieces of scientific literature.
Agonist
Antagonist
Control
TLR2 Overexpression Lysate
Publications
Read Publications using
NB100-56722 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28219705, 29501923). Rat reactivity reported in scientific literature (PMID: 29501923).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR2 Antibody (TL2.1)

  • CD282 antigen
  • CD282
  • TIL4CD282
  • TLR2
  • toll/interleukin 1 receptor-like 4
  • Toll/interleukin-1 receptor-like protein 4
  • toll-like receptor 2

Background

The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-29328
Species: Hu, Mu, Rt, Xp, Ye, Ze
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Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
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Species: Hu, Mu, Rt
Applications: WB, DB, Flow, IHC, IHC-P, Flow-CS, Flow-IC
NBP2-93743
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24875
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, BA, B/N, Flow-IC
NB100-56536
Species: Hu
Applications: WB, Flow, IHC, IHC-P, Flow-CS, Flow-IC
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-24787
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Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-CS, Flow-IC
NBP2-67360
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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB100-524
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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NB100-56655
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-24906
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, BA, Flow-IC
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Publications for TLR2 Antibody (NB100-56722)(60)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 11 applications: B/N, DB, ELISA, FLOW, Flow, Flow-CS, Func, ICC/IF, IP, In vitro, WB.


Filter By Application
B/N
(6)
DB
(1)
ELISA
(1)
FLOW
(6)
Flow
(10)
Flow-CS
(5)
Func
(1)
ICC/IF
(3)
IP
(1)
In vitro
(1)
WB
(6)
All Applications
Filter By Species
Human
(25)
Mouse
(3)
Rat
(1)
Canine
(2)
All Species
Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using NB100-56722 Applications Species
The E, Yao Q, Zhang P et al. Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells Cells. [PMID: 32046115] (WB, Human) WB Human
Ji X, Zhang X, Li H et al. Nfa34810 facilitates Nocardia farcinica invasion of host cells and stimulates TNF-alpha secretion through activation of the NF-kappa B and MAPK pathways via TLR4 Infect Immun. [PMID: 31964749] (ELISA, Mouse) ELISA Mouse
Akhter N, Madhoun A, Arefanian H et al Oxidative Stress Induces Expression of the Toll-Like Receptors (TLRs) 2 and 4 in the Human Peripheral Blood Mononuclear Cells: Implications for Metabolic Inflammation Cell. Cell Physiol Biochem. 2019 [PMID: 31162913] (Flow, Human)

Details:
Citation using the PE version of this antibody.
Flow Human
de Tymowski, C;Heming, N;Correia, MDT;Abbad, L;Chavarot, N;Le Stang, MB;Flament, H;Bex, J;Boedec, E;Bounaix, C;Soler-Torronteras, R;Denamur, E;Galicier, L;Oksenhendler, E;Fehling, HJ;Pinheiro da Silva, F;Benhamou, M;Monteiro, RC;Ben Mkaddem, S; CD89 Is a Potent Innate Receptor for Bacteria and Mediates Host Protection from Sepsis Cell Rep Apr 16 2019 [PMID: 30995475] (WB, Mouse) WB Mouse
Coelho da Silva FD, Covre LP, Stringari LL et al. Toll-like receptors blocking restores in vitro microbicidal activity in latent tuberculosis-infected subjects Int. J. Tuberc. Lung Dis. Feb 1 2019 [PMID: 30808454] (B/N, Human) B/N Human
Haruna T, Kariya S, Fujiwara T et al. Role of whole saliva in the efficacy of sublingual immunotherapy in seasonal allergic rhinitis Allergol Int Aug 27 2018 [PMID: 30166059] (Flow, Human) Flow Human
Kolodzinska A, Czarzasta K, Szczepankiewicz B et al. Toll-like receptor expression and apoptosis morphological patterns in female rat hearts with takotsubo syndrome induced by isoprenaline. Life Sci 2018 Apr 15 [PMID: 29501923] (Rat) Rat
Li F, Song R, Ao L et al. ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells Arterioscler thromb Vasc Biol 2017 May 25 [PMID: 28546218] (B/N, WB, Human)

Details:
This reference used the Azide Free version of NB100-56722.
B/N, WB Human
Hamidi S. Molecular mechanisms of the dermal immune response: cooperation between Toll-like receptors and Sphingosine-1-phosphate in fibroblasts. Thesis. 2014 (ICC/IF, Human)

Details:
TLR2 antibody used for ICC-IF in experiments involving primary cultures of normal human dermal fibroblasts isolated from human juvenile foreskin.
ICC/IF Human
Subramanian K1, Bergman P, Henriques-Normark B. Vitamin D Promotes Pneumococcal Killing and Modulates Inflammatory Responses in Primary Human Neutrophils J Innate Immun. J Innate Immun. 2017 Feb 28 [PMID: 28241127] (Flow, Human)

Details:
This citation used the Alexa Fluor 488 version of this antibody.
Flow Human
Show All 60 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR2 Antibody (NB100-56722) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56722AF350
Alexa Fluor 405 NB100-56726AF405
Alexa Fluor 488 NB100-56726AF488
Alexa Fluor 532 NB100-56722AF532
Alexa Fluor 594 NB100-56722AF594
Alexa Fluor 647 NB100-56726AF647
Alexa Fluor 700 NB100-56726AF700
Alexa Fluor 750 NB100-56722AF750
Biotin NB100-56056
DyLight 350 NB100-56726UV
DyLight 405 NB100-56726V
DyLight 488 NB100-56726G
DyLight 550 NB100-56726R
DyLight 594 NB100-56722DL594
DyLight 650 NB100-56726C
DyLight 680 NB100-56726FR
DyLight 755 NB100-56726IR
FITC NB100-56726F
Janelia Fluor 549 NB100-56722JF549
Janelia Fluor 646 NB100-56722JF646
PE NBP2-24909
PerCP NB100-56726PCP

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Research Areas for TLR2 Antibody (NB100-56722)

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Blogs on TLR2.

Toll-like receptor 2 activation contributes to oral squamous cell carcinoma development and miRNA-mediated drug resistance
By Jamshed Arslan, Pharm. D., PhD. Squamous cell carcinoma is the most common cancer in the oral cavity.1 The tumor surface biofilms in oral cancers contain high levels of aerobic and anaerobic microorganisms.1,2 Peri...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR1
TLR1 belongs to the Toll-like receptor (TLR) family, and is a key player in the recognition of pathogens as well as the activation of the innate immunity system. TLRs are highly conserved proteins with a high degree of structural and functional homolo...  Read full blog post.

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Bioinformatics

Gene Symbol TLR2