TLR2 Antibody (TL2.1) - BSA Free


Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 more
Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Expression of TLR2 was determined by western blotting. The immunoreactivity of the anti-TLR2 was confirmed with human intestinal lysate. Data are representative for more
Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Total protein from human THP-1 and U937 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The more
Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Monitoring of TLR2 expression in normal B cells and primary MCL cells cultured for 24h in the absence/presence of CD40L, IL-4, and TLR ligands. Filled curves refer more
Flow (Cell Surface): TLR2 Antibody (TL2.1) [NB100-56722] - Flow (Cell Surface): - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on stable transfected cell line using this more
Block/Neutralize: TLR2 Antibody (TL2.1) [NB100-56722] - Human monocyte-derived DCs were pre-incubated wit TLR-neutralizing antibodies including TLR2 antibody (NB100-56722) for 1 h. Afterwards, DCs were infected with H. more

Product Details

Reactivity Hu, Mu, Rt, Ca, RbSpecies Glossary
Applications WB, DB, ELISA, Flow, Func, ICC/IF, IP, B/N
BSA Free
1.0 mg/ml

Order Details

TLR2 Antibody (TL2.1) - BSA Free Summary

Additional Information
Clone TL2.1 was used by HLDA to establish CD designation.
This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (PMID: 10657659). The hybridoma supernatants were selected by flow cytometry.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Block/Neutralize reported in scientific literature (PMID 25412776)
  • Dot Blot reported in scientific literature (PMID 27248820)
  • ELISA reported in multiple pieces of scientific literature
  • Flow (Cell Surface) reported in scientific literature (PMID 24836676)
  • Flow Cytometry 1 ug/10^6 cells
  • Functional reported in scientific literature (PMID 25412776)
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 2-5 ug
  • In vitro assay reported in scientific literature (PMID 24512642)
  • Western Blot 1 - 2 ug/ml
TLR2 Overexpression Lysate
Read Publications using
NB100-56722 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28219705, 29501923). Rat reactivity reported in scientific literature (PMID: 29501923).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for TLR2 Antibody (TL2.1) - BSA Free

  • CD282 antigen
  • CD282
  • TIL4CD282
  • TLR2
  • toll/interleukin 1 receptor-like 4
  • Toll/interleukin-1 receptor-like protein 4
  • toll-like receptor 2


The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
Species: Hu
Applications: ELISA
Species: Ca, Eq, Hu, Pm, Mu, Pm, Rt
Applications: B/N, CyTOF-ready, DB, ELISA, Flow-IC, Flow, Func, ICC/IF, IHC, IHC-P, IP, In vitro, KD, Simple Western, WB
Species: Bv, Hu, Mu, Rt, Xp, Ye, Ze
Applications: BindInhib, B/N, ELISA, Flow, Func-Inh, IHC, In vitro, In vivo
Species: Mu
Applications: ELISA
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
Species: Hu, Mu, Rt
Applications: DB, Flow-CS, Flow-IC, Flow, IHC, IHC-P, WB
Species: Hu
Applications: ELISA
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
Species: Ca, Hu, Mu
Applications: BA, B/N, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: Flow-CS, Flow-IC, Flow, IHC, IHC-P, WB
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
Species: Ca, Hu, Mu
Applications: DB, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: Bind
Species: Hu
Applications: ELISA
Species: Hu, Mu, Rt
Applications: BA, DB, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
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Publications for TLR2 Antibody (NB100-56722)(62)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Rabbit.

We have publications tested in 10 applications: B/N, Cytometric Bead Assay Standard, ELISA, FLOW, Flow-CS, Func, ICC/IF, IP, In vitro, WB.

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Cytometric Bead Assay Standard
In vitro
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Showing Publications 1 - 10 of 62. Show All 62 Publications.
Publications using NB100-56722 Applications Species
Chenwen X, Quanan J, Yee H, et al Efficacy of Rg1-Oil Adjuvant on Inducing Immune Responses against Bordetella bronchiseptica in Rabbits J Immunol Res 2021-02-12 [PMID: 33575363] (WB, Rabbit)

Citation using the PE version of this antibody.
WB Rabbit
The E, Yao Q, Zhang P et al. Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells Cells. [PMID: 32046115] (WB, Human) WB Human
Ji X, Zhang X, Li H et al. Nfa34810 facilitates Nocardia farcinica invasion of host cells and stimulates TNF-alpha secretion through activation of the NF-kappa B and MAPK pathways via TLR4 Infect Immun. [PMID: 31964749] (ELISA, Mouse) ELISA Mouse
Akhter N, Madhoun A, Arefanian H et al Oxidative Stress Induces Expression of the Toll-Like Receptors (TLRs) 2 and 4 in the Human Peripheral Blood Mononuclear Cells: Implications for Metabolic Inflammation Cell. Cell Physiol Biochem. 2019-01-01 [PMID: 31162913] (FLOW, Human)

Citation using the PE version of this antibody.
FLOW Human
de Tymowski, C;Heming, N;Correia, MDT;Abbad, L;Chavarot, N;Le Stang, MB;Flament, H;Bex, J;Boedec, E;Bounaix, C;Soler-Torronteras, R;Denamur, E;Galicier, L;Oksenhendler, E;Fehling, HJ;Pinheiro da Silva, F;Benhamou, M;Monteiro, RC;Ben Mkaddem, S; CD89 Is a Potent Innate Receptor for Bacteria and Mediates Host Protection from Sepsis Cell Rep 2019-04-16 [PMID: 30995475] (WB, Mouse) WB Mouse
Haruna T, Kariya S, Fujiwara T et al. Role of whole saliva in the efficacy of sublingual immunotherapy in seasonal allergic rhinitis Allergol Int 2018-08-27 [PMID: 30166059] (FLOW, Human) FLOW Human
Kolodzinska A, Czarzasta K, Szczepankiewicz B et al. Toll-like receptor expression and apoptosis morphological patterns in female rat hearts with takotsubo syndrome induced by isoprenaline. Life Sci 2018-04-15 [PMID: 29501923] (Rat) Rat
Hamidi S. Molecular mechanisms of the dermal immune response: cooperation between Toll-like receptors and Sphingosine-1-phosphate in fibroblasts. Thesis. 2014-01-01 (ICC/IF, Human)

TLR2 antibody used for ICC-IF in experiments involving primary cultures of normal human dermal fibroblasts isolated from human juvenile foreskin.
ICC/IF Human
Ji X, Zhang X et al The Heparin-Binding Hemagglutinin of Nocardia cyriacigeorgica GUH-2 Stimulates Inflammatory Cytokine Secretion Through Activation of Nuclear Factor kappa B and Mitogen-Activated Protein Kinase Pathways via TLR4 Front Cell Infect Microbiol 2020-03-03 [PMID: 32117792] (WB, Human)

Citation using the Azide Free version of this antibody.
WB Human
Coelho da Silva FD, Covre LP, Stringari LL et al. Toll-like receptors blocking restores in vitro microbicidal activity in latent tuberculosis-infected subjects Int. J. Tuberc. Lung Dis. 2019-02-01 [PMID: 30808454] (B/N, Human) B/N Human
Show All 62 Publications.

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Product General Protocols

View specific protocols for TLR2 Antibody (NB100-56722): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies


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Blogs on TLR2. Showing 1-10 of 11 blog posts - Show all blog posts.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.

  Read full blog post.

Toll-like receptor 2 activation contributes to oral squamous cell carcinoma development and miRNA-mediated drug resistance
By Jamshed Arslan, Pharm. D., PhD. Squamous cell carcinoma is the most common cancer in the oral cavity.1 The tumor surface biofilms in oral cancers contain high levels of aerobic and anaerobic microorganisms.1,2 Peri...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
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Gene Symbol TLR2