TLR2 Antibody (TL2.1)

Images

 
Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Using the Biotin direct conjugate Detection of TLR2 mRNA in multiple cell lines. No detection was observed in L363 or Fravel cell lines. [PMID: 23593278]
Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control this antibody; red ...read more
Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using Azide Free version of NB100-56722. Intracellular TLR2 in 10^6 PBMs using 2 ug of TLR2 antibody. Shaded histogram represents cells without antibody; ...read more
Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using Azide/BSA FREE version of NB100-56726. Cell surface flow analysis of TLR2 in 10^6 PBMCs using 1 ug of this antibody. Shaded histogram represents cells ...read more
Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 ...read more
Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Staining of TLR2 in 10^6 ThP1 cells using 2 ug of this antibody. The closed histogram represents cells alone, green ...read more
Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Staining of TLR2 in 10^6 PBMCs (lymphocyte-gated) using 2 ugs ofMouse IgG2a Kappa, and red represents TLR2 antibody.

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, B/N, Flow, Flow-CS, Func, ICC/IF, IHC-P, IP
Clone
TL2.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TLR2 Antibody (TL2.1) Summary

Immunogen
This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (Flo et al, 2000). The hybridoma supernatants were selected by flow cytometry.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TLR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Blocking/Neutralizing
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:20-1:1000
  • Immunoprecipitation 2-5 ug
  • Flow (Cell Surface)
Application Notes
Immunocytochemistry/Immunofluorescence: please see Mempel et al. (2003) for details Immunohistochemistry (paraffin): please see Faure et al. (2001) for details Western Blot: please see Faure et al. (2001) for details Use in Functional and Blocking/ neutralizing reported in scientific literature (PMID 25412776). Use in FLOW cell surface reported in scientific literature (PMID 24836676).
Agonist
Antagonist
Positive Control
TLR2 Lysate (NBL1-16952)
Publications
Read Publications using
NB100-56722 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR2 Antibody (TL2.1)

  • CD282 antigen
  • CD282
  • TIL4CD282
  • TLR2
  • toll/interleukin 1 receptor-like 4
  • Toll/interleukin-1 receptor-like protein 4
  • toll-like receptor 2

Background

The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TLR2 Antibody (NB100-56722)(58)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 11 applications: B/N, DB, FLOW, Flow, Flow-CS, Func, ICC/IF, IHC-P, IP, In vitro, WB.


Filter By Application
B/N
(5)
DB
(1)
FLOW
(10)
Flow
(8)
Flow-CS
(6)
Func
(1)
ICC/IF
(3)
IHC-P
(1)
IP
(1)
In vitro
(1)
WB
(5)
All Applications
Filter By Species
Human
(26)
Mouse
(1)
Canine
(2)
All Species
Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using NB100-56722 Applications Species
Li F, Song R, Ao L et al. ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells Arterioscler thromb Vasc Biol 2017 May 25 [PMID: 28546218] (B/N, WB, Human)

Details:
This reference used the Azide Free version of NB100-56722.
B/N, WB Human
Subramanian K1, Bergman P, Henriques-Normark B. Vitamin D Promotes Pneumococcal Killing and Modulates Inflammatory Responses in Primary Human Neutrophils J Innate Immun. J Innate Immun. 2017 Feb 28 [PMID: 28241127] (Flow, Human)

Details:
This citation used the Alexa Fluor 488 version of this antibody.
Flow Human
Damien P, Cognasse F, Payrastre B et al. NF-kB Links TLR2 and PAR1 to Soluble Immunomodulator Factor Secretion in Human Platelets. Front Immunol. Feb 21 2017 [PMID: 28220122] (WB, Flow, Human) WB, Flow Human
Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J Allergy Clin Immunol. [PMID: 28219705] (Flow, Mouse)

Details:
Citation using the Alexa Fluor 647 form of this antibody.
Flow Mouse
Lin SJ, Kuo ML, Hsiao HS, Lee PT, Azithromycin modulates immune response of Human monocyte-derived dendritic cells and CD4(+) T cells Int. Immunopharmacol. Sep 21 2016 [PMID: 27664570] (FLOW, Human) FLOW Human
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. Jul 5 2016 [PMID: 27248820] (DB, Human) DB Human
Mastorci K, Muraro E, Pasini E et al. Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma. PLoS ONE. 2016 April 29 [PMID: 27123851] (Flow, Human)

Details:
This citation used the FITC version of this antibody.
Flow Human
Wong CK, Chu IM, Hon KL et al. Aberrant Expression of Bacterial Pattern Recognition Receptor NOD2 of Basophils and Microbicidal Peptides in Atopic Dermatitis. Molecules. 2016 Apr 15 [PMID: 27077833] (FLOW, Human) FLOW Human
Nagat E, Oho T. Invasive Streptococcus mutans induces inflammatory cytokine production in human aortic endothelial cells via regulation of intracellular TLR2 and NOD2. Mol Oral Microbiol. 2016 Mar 23 [PMID: 27004566] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine)

Details:
Used the PE form of this antibody.
FLOW Canine
Show All 58 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR2 Antibody (NB100-56722) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-56056
DyLight 350 Labeled NB100-56726UV
DyLight 405 Labeled NB100-56726V
DyLight 488 Labeled NB100-56726G
DyLight 550 Labeled NB100-56726R
DyLight 650 Labeled NB100-56726C
DyLight 680 Labeled NB100-56726FR
DyLight 755 Labeled NB100-56726IR
PE Labeled NBP2-24909
PerCP Labeled NB100-56726PCP

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Bioinformatics Tool for TLR2 Antibody (NB100-56722)

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Blogs on TLR2.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR1
TLR1 belongs to the Toll-like receptor (TLR) family, and is a key player in the recognition of pathogens as well as the activation of the innate immunity system. TLRs are highly conserved proteins with a high degree of structural and functional homolo...  Read full blog post.

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Bioinformatics

Gene Symbol TLR2
Entrez