Imiquimod, TLR7 ligand


Imiquimod, TLR7 ligand [NBP2-26228] - 293T cells were transfected with pCMV/TLR7 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 5 ug/mL of Imiquimod (R-837) was added. Cells were more

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Reactivity Hu, MuSpecies Glossary
Applications Func
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

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Imiquimod, TLR7 ligand Summary

Imiquimod, TLR7 ligand is an imidazoquinoline amine analog to guanosine. As a synthetic molecule of the Imidazoquinoline family, it has potent immunostimulatory activity. Imiquimod has been shown to activate only TLR7. This activation is MyD88 dependant and leads to the induction of the transcription factor NF-kB.
Imiquimod is human/mouse TLR7 agonist.
Endotoxin Note
<0.001 EU/ug


  • Functional
  • In vitro assay
  • Ligand Activation
Application Notes
Formula: C14H16N4, HCl. Molecular weight: 276.8. This product is useful for activation of TLR7 and stimulation of TLR7 has been achieved with 5-10 ug/mL. Use in ligand activation, functional, and in vitro assays reported in scientific literature (PMID 25957979)
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NBP2-26228 in the following applications:

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NBP2-26228 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
NBP2-26228-0.125mg: 125 ug in 50 uL of DMSO (this product was formerly supplied in sterile water) NBP2-26228-1mg: 1 mg in 400 uL of DMSO.
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.


5 mg size will be provided as 5 x 1.0 mg vials.

Alternate Names for Imiquimod, TLR7 ligand

  • R 837
  • R837


Imiquimod, TLR7 ligand, an imidazoquinoline amine analog to guanosine, is an immune response modifier with potent indirect antiviral activity. The antiviral activity of imiquimod was first shown in guinea pigs infected with herpes simplex virus [1]. Imiquimod is now an approved treatment for external genital warts caused by human papillomavirus infection. This low molecular synthetic molecule induces the production of cytokines such as IFN-Alpha. Unlike R848, Imiquimod activates only TLR7 but not TLR8 [2]. This activation is MyD88-dependent and leads to the induction of the transcription factor NF-kB [3].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Imiquimod, TLR7 ligand (NBP2-26228)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 9 applications: B/N, Flow Cytometry Control, Func, In Vivo, In vitro, In-vitro, KD, LA, WB.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP2-26228 Applications Species
Liu Y, Diamond SL. Activation of Most Toll-Like Receptors in Whole Human Blood Attenuates Platelet Deposition on Collagen under Flow Journal of Immunology Research 2023-01-17 [PMID: 36703865] (B/N) B/N
Jalloh S, Olejnik J, Berrigan J et al. CD169-mediated restrictive SARS-CoV-2 infection of macrophages induces pro-inflammatory responses PLOS Pathogens 2022-10-24 [PMID: 36279285] (KD, WB) KD, WB
Ye H, Pan J, Gong E et al. Inhibitory Effect of Immunologically Activated Mesenchymal Stem Cells on Lung Cancer Cell Growth and Metastasis Cancer biotherapy & radiopharmaceuticals 2021-03-26 [PMID: 33769841]
Effects of TLR Ligands on the Expression of Cytokines and Possible Role of NFkB in its Process in the Theca of Chicken Follicles Kang Y, Nii T, Isobe N J Poult Sci [PMID: 32055188] (Func, Chicken) Func Chicken
Hinks TSC, Marchi E, Jabeen M et al. Activation and In Vivo Evolution of the MAIT Cell Transcriptome in Mice and Humans Reveals Tissue Repair Functionality Cell Rep 2019-09-17 [PMID: 31533045] (In Vivo, Mouse, Human) In Vivo Mouse, Human
Parthasarathy G, Philipp MT. Intracellular TLR7 is activated in human oligodendrocytes in response to Borrelia burgdorferi exposure. Neurosci. Lett. 2018-02-03 [PMID: 29408631] (Human) Human
Yabe-Wada T, Matsuba S, Takeda K et al. TLR signals posttranscriptionally regulate the cytokine trafficking mediator sortilin. Sci Rep. 2016-05-25 [PMID: 27220277] (Func, In vitro, Human, Mouse) Func, In vitro Human, Mouse
Li Q, Ma Y, Li L et al. Flagellin influences the expression of a variety of important cytokines and chemokines without affecting the immune status of umbilical cord mesenchymal stem cells. Mol Med Rep 2015-09-01 [PMID: 26330280] (In-vitro, Func) In-vitro, Func
Nohmi K, Tokuhara D, Tachibana D et al. Zymosan Induces Immune Responses Comparable with Those of Adults in Monocytes, Dendritic Cells, and Monocyte-Derived Dendritic Cells from Cord Blood. J. Pediatr. 2015-05-06 [PMID: 25957979] (LA, In vitro, Func, Human)

Zymosan, TLR2 ligand (Imgenex IMG-2212) was used for in-vitro stimulation experiments involving human heparinized cord or adult blood Monocytes, peripheral blood dendritic cells (DCs) and monocyte-derived DCs (MoDCs). Zymosan was employed at 1 ug/mL concentration on Monocytes as well as on MoDCs and at 0.5 ug/mL on DCs. See full text for experimental details and results.
LA, In vitro, Func Human
Zhang L, Liu D, Pu D et al. The TLR7 agonist Imiquimod promote the immunogenicity of msenchymal stem cells Biological Research. 2015-01-17 [PMID: 25654296] (Human)

Imiquimod, TLR7 ligand used for stimulating human umbilical cord mesenchymal stem cells (UCMSCs) in experiments involving study of the immunogenicity of MSCs- Imiquimod dissolved in DMSO at 2.5 mg/ml concentration to make a stock solution and then added the stock solution to 6 wells plates containing 1.5 x 10(5) cells in 2 ml medium at 10 ug/ml working concentration. Experiments also involved Imiquimod treatment to co-cultures of PBMC and UCMSC.
Show All 13 Publications.

Review for Imiquimod, TLR7 ligand (NBP2-26228) (1) 51

Average Rating: 5
(Based on 1 review)

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CommentsImiquimod was added into the medium at final concentration
of 10 μg/ml. Imiquimod enhanced the osteo-differentiation ability of mesenchymal stem cells.

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