Doublecortin Antibody (3E1)


Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, more
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 more
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, EqSpecies Glossary
Applications WB, ICC/IF, IHC
1 mg/ml

Doublecortin Antibody (3E1) Summary

Recombinant full length human Lis-A isoform of Doublecortin purified from E. coli. [UniProt# O43602]
Cytoplasm. Cell projection. Note: Localizes at neurite tips.
Immature Neuronal Marker
Protein G purified
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  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Western Blot 1:10000
Application Notes
This Doublecortin (3E1) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blot, where bands can be seen at approximately 35 and 45 kDa.
Theoretical MW
35-45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 50% glycerol (1:1)
5mM Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for Doublecortin Antibody (3E1)

  • DBCN
  • Doublecortex
  • Doublecortin
  • Doublin
  • FLJ51296
  • Lissencephalin-X
  • LISX
  • Lis-X
  • X-linked (doublecortin)


Doublecortin was originally discovered since defects in the gene encoding it are causative of X-linked lissencephaly, a rare group of brain malformations resulting in a smooth cerebral cortex caused by aberrant neuronal migration during development. The name Doublecortin comes from the unusual layering of the cortex in this form of lissencephaly, which appears to have a second deep cortical layer of neurons. This layer consists of neurons which did not migrate from the subventricular zone to the normal cortical layer. Patients with this defect suffer from seizures and mental retardation. The HGNC name for Doublecortin is DCX, and it is also known as Doublin, Lissencephalin-X, DBCN and Lis-X. Four proteins encoded by the DCX produce bands of about 35kDa and 45kDa on Western blots. The 45kDa form is known as Lis-XA while the smaller forms are generated by alternate transcription, are all missing the first 81 amino acids of Lis-XA, and are referred to as Lis-XB, Lis-XC, Lis-XD. There are minor amino acid sequence differences between these three smaller isoforms. All of these protein contain two so-called Doublecortin domains, each about 90 amino acids long, which are believed to function in binding to microtubules, a C-terminal serine and proline rich region which may become phosphorylated in vivo. The doublecortin protein appears to function as a microtubule and actin binding protein and may interact with Lis-1, a member of the beta-transducin or WD protein family, a protein mutations of which are also associated with lissencephaly. DCX is expressed very early in neuronal development, as neuroblasts become post-mitotic, but is lost as neurons mature. Developing neurons start to lose DCX expression about the time that they begin to express NeuN, a neuronal specific protein characteristic of more mature neurons, now known to correspond to the RNA binding protein Fox3. Antibodies to DCX are used to identify stem cells in sections and in tissue culture, and to see if neurogenesis is taking place.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP1-92684AF405
Alexa Fluor 488 NBP1-92684AF488
Alexa Fluor 647 NBP1-92684AF647
Alexa Fluor 700 NBP1-92684AF700
DyLight 350 NBP1-92684UV
DyLight 405 NBP1-92684V
DyLight 488 NBP1-92684G
DyLight 550 NBP1-92684R
DyLight 680 NBP1-92684FR
DyLight 755 NBP1-92684IR
FITC NBP1-92684F
Janelia Fluor 549 NBP1-92684JF549
Janelia Fluor 646 NBP1-92684JF646

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Gene Symbol DCX