Doublecortin Antibody (3E1)

Images

 
Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, ...read more
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 ...read more
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, EqSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
3E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Doublecortin Antibody (3E1) Summary

Immunogen
Recombinant full length human Lis-A isoform of Doublecortin purified from E. coli. [UniProt# O43602]
Localization
Cytoplasm. Cell projection. Note: Localizes at neurite tips.
Marker
Immature Neuronal Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
DCX
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
Application Notes
This Doublecortin (3E1) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where bands can be seen at approximately 35 and 45 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
49.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% Glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Doublecortin Antibody (3E1)

  • doublecortin
  • Doublin
  • FLJ51296
  • Lissencephalin-X
  • Lis-X
  • X-linked (doublecortin)

Background

Doublecortin was originally discovered since defects in the gene encoding it are causative of X-linked lissencephaly, a rare group of brain malformations resulting in a smooth cerebral cortex caused by aberrant neuronal migration during development. The name Doublecortin comes from the unusual layering of the cortex in this form of lissencephaly, which appears to have a second deep cortical layer of neurons. This layer consists of neurons which did not migrate from the subventricular zone to the normal cortical layer. Patients with this defect suffer from seizures and mental retardation. The HGNC name for Doublecortin is DCX, and it is also known as Doublin, Lissencephalin-X, DBCN and Lis-X. Four proteins encoded by the DCX produce bands of about 35kDa and 45kDa on Western blots. The 45kDa form is known as Lis-XA while the smaller forms are generated by alternate transcription, are all missing the first 81 amino acids of Lis-XA, and are referred to as Lis-XB, Lis-XC, Lis-XD. There are minor amino acid sequence differences between these three smaller isoforms. All of these protein contain two so-called Doublecortin domains, each about 90 amino acids long, which are believed to function in binding to microtubules, a C-terminal serine and proline rich region which may become phosphorylated in vivo. The doublecortin protein appears to function as a microtubule and actin binding protein and may interact with Lis-1, a member of the beta-transducin or WD protein family, a protein mutations of which are also associated with lissencephaly. DCX is expressed very early in neuronal development, as neuroblasts become post-mitotic, but is lost as neurons mature. Developing neurons start to lose DCX expression about the time that they begin to express NeuN, a neuronal specific protein characteristic of more mature neurons, now known to correspond to the RNA binding protein Fox3. Antibodies to DCX are used to identify stem cells in sections and in tissue culture, and to see if neurogenesis is taking place.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-92684AF405
Alexa Fluor 488 Labeled NBP1-92684AF488
Alexa Fluor 647 Labeled NBP1-92684AF647
Alexa Fluor 700 Labeled NBP1-92684AF700
DyLight 350 Labeled NBP1-92684UV
DyLight 405 Labeled NBP1-92684V
DyLight 488 Labeled NBP1-92684G
DyLight 680 Labeled NBP1-92684FR
DyLight 755 Labeled NBP1-92684IR
FITC Labeled NBP1-92684F

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Bioinformatics

Gene Symbol DCX
Entrez
Uniprot