TLR8 Antibody (44C143) - BSA Free

Images

 
Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Intracellular expression of TLR8 in monocyte-derived macrophages (MDMs). MDMs which were incubated with PE-conjugated anti-TLR8 or isotype antibody control. ...read more
Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Expression of TLR8 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol ...read more
Orthogonal Strategies: Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - All the cell lines expressed mRNA for TLR8 (Figure 1) and displayed a strong expression of TLR8 protein (Figure 2). Lysates of human ...read more
Immunohistochemistry-Paraffin: TLR8 Antibody (44C143) [NBP2-24917] - Formalin-fixed, paraffin-embedded human spleen stained with TLR8 antibody at 2 ug/ml.
Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - HEK cells expressing TLR8 were treated with ssRNA40-Alexa 546 followed by fixation, permeablization and staining for TLR8 using anti-TLR8 Alexa 488 conjugate or ...read more
Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - Analysis of TLR8 in human Ramos cell lysate. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: TLR8 Antibody (44C143) [NBP2-24917] - Simple Western lane view shows a specific band for TLR8 in 0.5 mg/ml of Ramos (left) and Human Spleen (right) lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications WB, Simple Western, DB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
44C143
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

TLR8 Antibody (44C143) - BSA Free Summary

Additional Information
Clone 44C143 was used by HLDA to establish CD designation.
Immunogen
This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Dot Blot reported in scientific literature (PMID 27248820)
  • Flow (Cell Surface) reported in multiple pieces of scientific literature
  • Flow (Intracellular) reported in reported in multiple pieces of scientific literature (PMID 24836676)
  • Flow Cytometry 0.5-1 ug/10^6 cells
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Simple Western 20 ug/ml
  • Western Blot 1-3 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. This antibody is CyTOF ready.
Agonist
Publications
Read Publications using
NBP2-24917 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.4
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR8 Antibody (44C143) - BSA Free

  • CD288 antigen
  • CD288
  • MGC119599
  • MGC119600
  • TLR8
  • toll-like receptor 8

Background

Toll-like receptor 8 (TLR8) is a member of the TLR family of receptors that play a role in innate immune system activation and the recognition of pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs) (1,2). TLRs are type I membrane receptors that can be expressed on either the cell surface or internally, on endosomes (1,2). TLR8 is an endosomal receptor and is activated by pathogenic single stranded (ss) RNA (1-3). TLR8 is located on the X chromosome and is expressed mostly in monocytes/macrophages, neutrophils, and myeloid dendritic cells (1-3). Structurally, TLR8 consists of an extracellular domain, a cysteine-rich region, and transmembrane domain, and a Toll/Interleukin-1 receptor homology (TIR) domain (3,4). The extracellular domain contains a N-terminal leucine rich repeat (LRRNT) and a C-terminal LRR (LRRCT) which have 26 LRRs between them, each approximately 20-30 amino acids (aa), and a Z-loop between LRR14 and LRR15 (3). The primary isoform of the human TLR8 is synthesized as a protein 1041 aa in length with a theoretical molecular weight of ~120 kDa (4).

TLR8 is highly similar to TLR7 and both pathways are mediated by the adapter protein MyD88 to signal through IFN regulatory factor 7 (IRF7) and nuclear factor (NF)-kappaB (1-3,5). However, TLR7 recognizes guanosine and GU-rich ssRNA, while TLR8 recognizes uridine and AU-rich sequences (2,5). TLR7/TLR8 agonists, including derivatives of the immunostimulatory imiquimod, have been shown to be a promising cancer therapy capable of providing anticancer signals to antigen presenting cells (APCs), with many agonists being tested in both pre-clinical and clinical trials (6). Similarly, studies suggest that agonists for TLR8, in combination with other individual TLR agonists and antagonists, may also be useful for treating inflammatory allergic diseases, such as allergic rhinitis (7).

References

1. Sakaniwa, K., & Shimizu, T. (2020). Targeting the innate immune receptor TLR8 using small-molecule agents. Acta crystallographica. Section D, Structural biology, 76(Pt 7). https://doi.org/10.1107/S2059798320006518

2. Cervantes, J. L., Weinerman, B., Basole, C., & Salazar, J. C. (2012). TLR8: the forgotten relative revindicated. Cellular & molecular immunology. https://doi.org/10.1038/cmi.2012.38

3. Ohto, U., Tanji, H., & Shimizu, T. (2014). Structure and function of toll-like receptor 8. Microbes and infection. https://doi.org/10.1016/j.micinf.2014.01.007

4. Uniprot (Q9NR97)

5. Jannuzzi, G. P., de Almeida, J., Paulo, L., de Almeida, S. R., & Ferreira, K. S. (2020). Intracellular PRRs Activation in Targeting the Immune Response Against Fungal Infections. Frontiers in cellular and infection microbiology. https://doi.org/10.3389/fcimb.2020.591970

6. Frega, G., Wu, Q., Le Naour, J., Vacchelli, E., Galluzzi, L., Kroemer, G., & Kepp, O. (2020). Trial Watch: experimental TLR7/TLR8 agonists for oncological indications. Oncoimmunology. https://doi.org/10.1080/2162402X.2020.1796002

7. Golshiri-Isfahani, A., Amizadeh, M., & Arababadi, M. K. (2018). The roles of toll like receptor 3, 7 and 8 in allergic rhinitis pathogenesis. Allergologia et immunopathologia. https://doi.org/10.1016/j.aller.2017.09.026

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for TLR8 Antibody (NBP2-24917)(48)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 9 applications: Cytometric Bead Assay Standard, FLOW, Flow - IC, Flow Cytometry Control, Flow-CS, ICC/IF, IF/IHC, IHC-P, WB.


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Cytometric Bead Assay Standard
(1)
FLOW
(12)
Flow - IC
(1)
Flow Cytometry Control
(3)
Flow-CS
(2)
ICC/IF
(1)
IF/IHC
(2)
IHC-P
(3)
WB
(4)
All Applications
Filter By Species
Human
(22)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using NBP2-24917 Applications Species
Campbell GR, Rawat P, Teodorof-Diedrich C, Spector SA IRAK1 inhibition blocks the HIV-1 RNA mediated pro-inflammatory cytokine response from microglia The Journal of general virology 2023-05-01 [PMID: 37256770]
de Barros De Luccia T, Ono E, Menon R et al. The effect of Gestational Diabetes Mellitus on the fetal compartment Journal of Reproductive Immunology 2021-03-01 [PMID: 33836321] (IHC-P, Human) IHC-P Human
Li Y, Chen X, Jin R et al. Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs Science advances 2021-02-05 [PMID: 33627421] (WB, Mouse) WB Mouse
Menendez D, Snipe J, Marzec J et al. p53-responsive TLR8 SNP enhances human innate immune response to respiratory syncytial virus J. Clin. Invest. 2019-08-20 [PMID: 31430261] (FLOW) FLOW
Evankovich J, Lear T, Baldwin C et al. Toll-Like Receptor 8 Stability is Regulated by Ring Finger 216 in response to circulating MicroRNAs Am. J. Respir. Cell Mol. Biol. 2019-08-06 [PMID: 31385713]
Deng G, Ge J, Liu C et al. Impaired expression and function of TLR8 in chronic HBV infection and its association with treatment responses during peg-IFN-a-2a antiviral therapy. Clin Res Hepatol Gastroenterol. [PMID: 28236535] (IF/IHC)

Details:
Citation using the PE form of this antibody.
IF/IHC
Helminen O, Huhta H, Kauppila JH et al. Localization of nucleic acid-sensing toll-like receptors in human and mouse pancreas. APMIS. 2016-12-28 [PMID: 28028829] (IF/IHC, Human) IF/IHC Human
Helminen O, Huhta H, Lehenkari Petri P. Nucleic acid-sensing Toll-like receptors 3, 7 and 8 in esophageal epithelium, Barrett's esophagus, dysplasia and adenocarcinoma Oncoimmunology 2016-07-29 [PMID: 27467941]
Huhta H, Helminen O, Kauppila JH et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer J. Histochem. Cytochem. 2016-08-01 [PMID: 27370795] (IHC-P, Human) IHC-P Human
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. 2016-07-05 [PMID: 27248820] (Cytometric Bead Assay Standard, Human) Cytometric Bead Assay Standard Human
Show All 48 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR8 Antibody (NBP2-24917). (Showing 1 - 2 of 2 FAQs).

  1. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
    • These antibodies are all purified from tissue culture supernatant.
  2. Do you have any reference paperd to show this antibody (clone 44C143) reacts with mouse tissues?
    • Yes we do indeed have several papers cited with use in Mouse. I am not certain about your application so I am going to note the links to all of the papers that used the antibody in mouse.http://www.ncbi.nlm.nih.gov/pubmed/16446426https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365979/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518890/I also have performed a blast comparison between the human immunogen sequence and the mouse and found it has 70 % homology. we would recommend the homology to be higher than 85% for predicting whether an antibody will work however there must be enough homology in the actual epitope.
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Bioinformatics

Gene Symbol TLR8