TLR8 Antibody (44C143)

Images

 
Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - Analysis of TLR8 in human Ramos cell lysate. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: TLR8 Antibody (44C143) [NBP2-24917] - Formalin-fixed, paraffin-embedded human spleen stained with TLR8 antibody at 2 ug/ml.
Flow (Intracellular): TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the PE conjugate of NBP2-24917. Staining of TLR8 in Ramos cells using 2 ug of NBP2-24817. Shaded histogram represents Ramos cells without ...read more
Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Intracellular flow analysis of 10^6 human lymphocytes using 1 ug of TLR8 antibody. Shaded histogram represents cells without antibody; green represents isotype ...read more
Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the FITC conjugate of NBP2-24917. Staining of TLR8 in 10^6 human lymphocytes using 1 ug of this antibody. The shaded histogram represents lymphocytes ...read more
Flow (Intracellular): TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the FITC conjugate of NBP2-24917. Staining of TLR8 in 10^6 Ramos cells using 0.5 ugs of this antibody. The shaded histogram represents Ramos ...read more
Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the PE conjugate of NBP2-24917. Staining of TLR8 in 10^6 human PBMCs using 0.5 ug of NBP2-24817, 0.25 ug of anti-human CD14, and 0.5 ug of isotype ...read more
Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the PE conjugate of NBP2-24917. Staining of TLR8 in BALB/c mouse splenocytes (lymphocyte gate) using 2 ug of this antibody. Green represents isotype ...read more
Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the FITC conjugate of NBP2-24917. Staining of CD14-/CD11c+/TLR8+ myeloid dendritic cells (mDCs). Cells were stained for surface markers CD14 and ...read more
Flow (Intracellular): TLR8 Antibody (44C143) [NBP2-24917] - An intracellular stain was performed on A549 cells with TLR8 (44C143) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). ...read more
Simple Western: TLR8 Antibody (44C143) [NBP2-24917] - Simple Western lane view shows a specific band for TLR8 in 0.5 mg/ml of Ramos (left) and Human Spleen (right) lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, DB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
44C143
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TLR8 Antibody (44C143) Summary

Immunogen
This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Simple Western 20 ug/ml
  • Dot Blot
  • Flow Cytometry 0.5-1 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow (Intracellular)
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in Flow-intracellular reported in scientific literature (PMID 24836676). Use in Flow-cell surface reported in multiple pieces of scientific literature. Use in Dot blot reported in scientific literature (PMID: 27248820).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Agonist
Publications
Read Publications using
NBP2-24917 in the following applications:

Reactivity Notes

This antibody is reactive to Human, Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.4
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR8 Antibody (44C143)

  • CD288 antigen
  • CD288
  • MGC119599
  • MGC119600
  • TLR8
  • toll-like receptor 8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24906
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, Flow-IC, IF
NBP2-24729
Species: Hu, Mu, Rt, Ca, Eq, Mk, Pm
Applications: WB, Simple Western, DB, ELISA, Flow, Func, ICC/IF, IHC, IHC-P, IP, In vitro, B/N, CyTOF-ready, Flow-IC
NBP2-24875
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NB100-56566
Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, B/N, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC, KO
NB100-56722
Species: Hu, Mu, Rt, Ca
Applications: WB, DB, ELISA, Flow, Func, ICC/IF, IP, In vitro, B/N, Flow-CS
7270-IL/CF
Species: Hu
NB100-56563
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, IHC, IHC-P, Flow-IC
NBP2-24787
Species: Hu, Mu, Ca
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-CS, Flow-IC
NBP2-75930
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-56536
Species: Hu
Applications: WB, Flow, IHC, IHC-P, Flow-CS, Flow-IC
NBP2-29328
Species: Hu, Mu, Rt, Xp, Ye, Ze
Applications: ELISA
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC, IHC-P, KO
DDX0490P-100
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC, IF
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45678
Species: Hu
Applications: WB, IHC, IF
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-24917
Species: Hu, Mu
Applications: WB, Simple Western, DB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-P, CyTOF-ready
Supplier Logo

Publications for TLR8 Antibody (NBP2-24917)(52)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 10 applications: DB, FLOW, Flow, Flow - IC, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-P, WB.


Filter By Application
DB
(1)
FLOW
(11)
Flow
(7)
Flow - IC
(1)
Flow-CS
(2)
Flow-IC
(3)
ICC/IF
(1)
IHC
(2)
IHC-P
(2)
WB
(3)
All Applications
Filter By Species
Human
(26)
All Species
Showing Publications 1 - 10 of 52. Show All 52 Publications.
Publications using NBP2-24917 Applications Species
Menendez D, Snipe J, Marzec J et al. p53-responsive TLR8 SNP enhances human innate immune response to respiratory syncytial virus J. Clin. Invest. Aug 20 2019 [PMID: 31430261] (Flow) Flow
Jamin A, Dehoux L, Dossier C et al. Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome Clin Exp Immunol (FLOW, Human)

Details:
This publication used the PE conjugated form of this antibody (Cat# NBP2-24817).
FLOW Human
Evankovich J, Lear T, Baldwin C et al. Toll-Like Receptor 8 Stability is Regulated by Ring Finger 216 in response to circulating MicroRNAs Am. J. Respir. Cell Mol. Biol. Aug 6 2019 [PMID: 31385713]
Deng G, Ge J, Liu C et al. Impaired expression and function of TLR8 in chronic HBV infection and its association with treatment responses during peg-IFN-a-2a antiviral therapy. Clin Res Hepatol Gastroenterol. [PMID: 28236535] (IHC)

Details:
Citation using the PE form of this antibody.
IHC
Helminen O, Huhta H, Kauppila JH et al. Localization of nucleic acid-sensing toll-like receptors in human and mouse pancreas. APMIS. Dec 28 2016 [PMID: 28028829] (IHC, Human) IHC Human
Huhta H, Helminen O, Kauppila JH et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer J. Histochem. Cytochem. 2016 Aug 01 [PMID: 27370795] (IHC-P, Human) IHC-P Human
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. Jul 5 2016 [PMID: 27248820] (DB, Human) DB Human
Wang CH, Eng HL, Lin KH et al. Functional Polymorphisms of TLR8 Are Associated With Hepatitis C Virus Infection Immunology (FLOW, Human)

Details:
This publication used the PE conjugated form of this antibody (Cat# NBP2-24817).
FLOW Human
Vatakis D, Nixon C, Bristol G, Zack J. Differentially stimulated CD4+ T cells display altered human immunodeficiency virus infection kinetics: implications for the efficacy of antiviral agents J Virol (FLOW)

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP2-24972).
FLOW
Jamin A, Dehoux L, Dossier C et al. Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome Clin Exp Immunol 2015 Jun 30 [PMID: 26123900] (Flow, Human)

Details:
This citation used the PE version of this antibody.
Flow Human
Show All 52 Publications.

Reviews for TLR8 Antibody (NBP2-24917) (0)

There are no reviews for TLR8 Antibody (NBP2-24917). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR8 Antibody (NBP2-24917). (Showing 1 - 2 of 2 FAQs).

  1. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
    • These antibodies are all purified from tissue culture supernatant.
  2. Do you have any reference paperd to show this antibody (clone 44C143) reacts with mouse tissues?
    • Yes we do indeed have several papers cited with use in Mouse. I am not certain about your application so I am going to note the links to all of the papers that used the antibody in mouse.http://www.ncbi.nlm.nih.gov/pubmed/16446426https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365979/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518890/I also have performed a blast comparison between the human immunogen sequence and the mouse and found it has 70 % homology. we would recommend the homology to be higher than 85% for predicting whether an antibody will work however there must be enough homology in the actual epitope.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-24917AF350
Alexa Fluor 405 NBP2-24917AF405
Alexa Fluor 488 NBP2-24917AF488
Alexa Fluor 532 NBP2-24917AF532
Alexa Fluor 594 NBP2-24917AF594
Alexa Fluor 647 NBP2-24917AF647
Alexa Fluor 700 NBP2-24917AF700
Alexa Fluor 750 NBP2-24917AF750
Allophycocyanin NBP2-24917APC
DyLight 350 NBP2-24917UV
DyLight 405 NBP2-24917V
DyLight 488 NBP2-24917G
DyLight 550 NBP2-24917R
DyLight 594 NBP2-24917DL594
DyLight 650 NBP2-24917C
DyLight 680 NBP2-24917FR
DyLight 755 NBP2-24917IR
FITC NBP2-24917F
HRP NBP2-24917H
Janelia Fluor 549 NBP2-24917JF549
Janelia Fluor 646 NBP2-24917JF646
PE NBP2-24817
PerCP NBP2-24917PCP

Additional TLR8 Products

Bioinformatics Tool for TLR8 Antibody (NBP2-24917)

Discover related pathways, diseases and genes to TLR8 Antibody (NBP2-24917). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TLR8 Antibody (NBP2-24917)

Discover more about diseases related to TLR8 Antibody (NBP2-24917).
 

Pathways for TLR8 Antibody (NBP2-24917)

View related products by pathway.

PTMs for TLR8 Antibody (NBP2-24917)

Learn more about PTMs related to TLR8 Antibody (NBP2-24917).
 

Research Areas for TLR8 Antibody (NBP2-24917)

Find related products by research area.

Blogs on TLR8

There are no specific blogs for TLR8, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TLR8 Antibody (44C143) and receive a gift card or discount.

Bioinformatics

Gene Symbol TLR8