Recombinant Human IFN-beta Protein

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Recombinant Human IFN‑ beta (Catalog # 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5.00-30.0 ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IFN-beta Protein Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 5.00-30.0 pg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein
Met22-Asn187
Accession #
N-terminal Sequence
Met22 & Ser23
Protein/Peptide Type
Recombinant Proteins
Gene
IFNB1
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20-26 kDa, reducing conditions
Publications
Read Publications using
8499-IF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-beta Protein

  • Fibroblast interferon
  • IFB
  • IFBIFNB
  • IFF
  • IFNB
  • IFNB1
  • IFNbeta
  • IFN-beta
  • interferon beta
  • interferon, beta 1, fibroblast
  • MGC96956

Background

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta , but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
  6. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
  7. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
  8. Matikainen, S. et al. (1999) Blood 93:1980.
  9. Teige, I. et al. (2003) J. Immunol. 170:4776.
  10. Shinohara, M.L. et al. (2008) Immunity 29:68.
  11. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  12. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  13. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  14. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.

Publications for IFN-beta (8499-IF)(44)

We have publications tested in 4 confirmed species: Human, Mouse, Primate - C. aethiops,  Human.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, Spheroid Culture, Stimulation.


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Bioassay
(37)
Cell Culture
(3)
Differentiation
(2)
Spheroid Culture
(1)
Stimulation
(2)
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Human
(42)
Mouse
(1)
Primate - C. aethiops
(1)
 Human
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Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using 8499-IF Applications Species
Li, WW;Fan, XX;Zhu, ZX;Cao, XJ;Zhu, ZY;Pei, DS;Wang, YZ;Zhang, JY;Wang, YY;Zheng, HX; Tyrosine phosphorylation of IRF3 by BLK facilitates its sufficient activation and innate antiviral response PLoS pathogens 2023-10-01 [PMID: 37871014] (Bioassay, Human) Bioassay Human
Twarog, M;Schustak, J;Xu, Y;Coble, M;Dolan, K;Esterberg, R;Huang, Q;Saint-Geniez, M;Bao, Y; TNF? induced by DNA-sensing in macrophage compromises retinal pigment epithelial (RPE) barrier function Scientific reports 2023-09-02 [PMID: 37660150] (Bioassay, Human) Bioassay Human
Wang, Y;Ninaber, DK;Faiz, A;van der Linden, AC;van Schadewijk, A;Lutter, R;Hiemstra, PS;van der Does, AM;Ravi, A; Acute cigarette smoke exposure leads to higher viral infection in human bronchial epithelial cultures by altering interferon, glycolysis and GDF15-related pathways Respiratory research 2023-08-23 [PMID: 37612597] (Bioassay, Human) Bioassay Human
Huntzinger, E;Sinteff, J;Morlet, B;Séraphin, B; HELZ2: a new, interferon-regulated, human 3'-5' exoribonuclease of the RNB family is expressed from a non-canonical initiation codon Nucleic acids research 2023-08-21 [PMID: 37602378] (Bioassay, Human) Bioassay Human
Ma, X;Wang, Q;Sun, C;Agarwal, I;Wu, H;Chen, J;Zhao, C;Qi, G;Teng, Q;Yuan, C;Yan, S;Peng, J;Li, R;Song, K;Zhang, Q;Kong, B; Targeting TCF19 sensitizes MSI endometrial cancer to anti-PD-1 therapy by alleviating CD8+ T cell exhaustion via TRIM14-IFN-? axis Cell reports 2023-08-09 [PMID: 37566545] (Bioassay, Human) Bioassay  Human
Nigam, N;Bernard, B;Sevilla, S;Kim, S;Dar, MS;Tsai, D;Robbins, Y;Burkitt, K;Sievers, C;Allen, CT;Bennett, RL;Tettey, TT;Carter, B;Rinaldi, L;Lingen, MW;Sater, H;Edmondson, EF;Moshiri, A;Saeed, A;Cheng, H;Luo, X;Brennan, K;Koparde, V;Chen, C;Das, S;Andresson, T;Abdelmaksoud, A;Murali, M;Sakata, S;Takeuchi, K;Chari, R;Nakamura, Y;Uppaluri, R;Sunwoo, JB;Van Waes, C;Licht, JD;Hager, GL;Saloura, V; SMYD3 represses tumor-intrinsic interferon response in HPV-negative squamous cell carcinoma of the head and neck Cell reports 2023-07-17 [PMID: 37463106] (Cell Culture, Human) Cell Culture Human
A Ta, R Ricci-Azev, SO Vasudevan, SS Wright, P Kumari, MS Havira, M Surendran, VA Rathinam, SK Vanaja A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3 Nature Communications, 2023-04-11;14(1):2035. 2023-04-11 [PMID: 37041208] (Bioassay, Human) Bioassay Human
J Nassour, LG Aguiar, A Correia, TT Schmidt, L Mainz, S Przetocka, C Haggblom, N Tadepalle, A Williams, MN Shokhirev, SC Akincilar, V Tergaonkar, GS Shadel, J Karlseder Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis Nature, 2023-02-08;614(7949):767-773. 2023-02-08 [PMID: 36755096] (Bioassay, Human) Bioassay Human
WC Ng, SS Kwek, B Sun, M Yousefi, EZ Ong, HC Tan, AS Puschnik, KR Chan, YS Ooi, EE Ooi A fast-growing dengue virus mutant reveals a dual role of STING in response to infection Open Biology, 2022-12-14;12(12):220227. 2022-12-14 [PMID: 36514984] (Bioassay, Human) Bioassay Human
MW Kenaston, OH Pham, MJ Petit, PS Shah Transcriptomic profiling implicates PAF1 in both active and repressive immune regulatory networks BMC Genomics, 2022-11-30;23(1):787. 2022-11-30 [PMID: 36451099] (Stimulation, Human) Stimulation Human
Show All 44 Publications.

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Additional IFN-beta Products

Blogs on IFN-beta.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ...  Read full blog post.

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Bioinformatics

Gene Symbol IFNB1
Uniprot