Recombinant Human IFN‑ beta (Catalog # 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5-30 pg/mL.
S.A approximately 2.8x10^8 IU/mg compared to WHO standard
Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 5-30 pg/mL. The specific activity of Recombinant Human IFN-beta is approximately 2.8 x 108 IU/mg, which is calibrated against human IFN- beta WHO International Standard (NIBSC code: 00/572).
Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein Met22-Asn187
<0.10 EU per 1 μg of the protein by the LAL method.
20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
>95%, by SDS-PAGE with silver staining.
Reconstitute at 200 μg/mL in sterile water.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human IFN-beta Protein
interferon, beta 1, fibroblast
Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta , but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).
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