Recombinant Human IFN-beta Protein

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Recombinant Human IFN‑ beta (Catalog # 8499-IF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5-30 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human IFN-beta Protein Summary

Additional Information
S.A approximately 2.8x10^8 IU/mg compared to WHO standard
Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 5-30 pg/mL.
The specific activity of Recombinant Human IFN-beta is approximately 2.8 x 108 IU/mg, which is calibrated against human IFN- beta WHO International Standard (NIBSC code: 00/572).
Source
Chinese Hamster Ovary cell line, CHO-derived human IFN-beta protein
Met22-Asn187
Accession #
N-terminal Sequence
Met22 & Ser23
Protein/Peptide Type
Recombinant Proteins
Gene
IFNB1
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20-26 kDa, reducing conditions
Publications
Read Publications using
8499-IF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-beta Protein

  • Fibroblast interferon
  • IFB
  • IFBIFNB
  • IFF
  • IFNB
  • IFNB1
  • IFNbeta
  • IFN-beta
  • interferon beta
  • interferon, beta 1, fibroblast
  • MGC96956

Background

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta , but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (5-8). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (9). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (10-14).
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
  6. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
  7. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
  8. Matikainen, S. et al. (1999) Blood 93:1980.
  9. Teige, I. et al. (2003) J. Immunol. 170:4776.
  10. Shinohara, M.L. et al. (2008) Immunity 29:68.
  11. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  12. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  13. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  14. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.

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8499-IF
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Applications: Bioactivity

Publications for IFN-beta (8499-IF)(29)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, Spheroid Culture, Stimulation.


Filter By Application
Bioassay
(24)
Cell Culture
(2)
Differentiation
(1)
Spheroid Culture
(1)
Stimulation
(1)
All Applications
Filter By Species
Human
(29)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using 8499-IF Applications Species
KK Jang, ME Kaczmarek, S Dallari, YH Chen, T Tada, J Axelrad, NR Landau, KA Stapleford, K Cadwell Variable susceptibility of intestinal organoid-derived monolayers to SARS-CoV-2 infection PloS Biology, 2022;20(3):e3001592. 2022 [PMID: 35358182] (Differentiation, Human) Differentiation Human
NL Ka, GY Lim, S Hwang, SS Kim, MO Lee IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer Cell Reports, 2021;37(12):110138. 2021 [PMID: 34936865] (Bioassay, Human) Bioassay Human
S Wüst, P Schad, S Burkart, M Binder Comparative Analysis of Six IRF Family Members in Alveolar Epithelial Cell-Intrinsic Antiviral Responses Cells, 2021;10(10):. 2021 [PMID: 34685580] (Stimulation, Human) Stimulation Human
C Winkler, M King, J Berthe, D Ferraioli, A Garuti, F Grillo, J Rodriguez-, L Ferrando, N Chopin, I Ray-Coquar, O Delpuech, D Bedognetti, A Ballestrer, E Leo, G Zoppoli SLFN11 captures cancer-immunity interactions associated with platinum sensitivity in high-grade serous ovarian cancer JCI Insight, 2021;6(18):. 2021 [PMID: 34549724] (Bioassay, Human) Bioassay Human
C Prelli Boz, R Nchioua, M Volcic, L Koepke, J Krüger, D Schütz, S Heller, CM Stürzel, D Kmiec, C Conzelmann, J Müller, F Zech, E Braun, R Gro beta , L Wettstein, T Weil, J Wei beta , F Diofano, AA Rodríguez, S Wiese, D Sauter, J Münch, C Goffinet, A Catanese, M Schön, TM Boeckers, S Stenger, K Sato, S Just, A Kleger, KMJ Sparrer, F Kirchhoff IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro Nature Communications, 2021;12(1):4584. 2021 [PMID: 34321474] (Bioassay, Human) Bioassay Human
X Yang, X Zhao, Y Zhu, Y Shen, Y Wang, P Lu, Z Jiang, H Pan, J Yang, J Xun, L Zhao, J Wang, Z Liang, X Shen, Y Liang, Q Lin, H Liang, L Jin, D Zhang, J Liu, B Wang, S Jiang, J Xu, H Wu, H Lu, H Zhu FKBP3 Induces Human Immunodeficiency Virus Type 1 Latency by Recruiting Histone Deacetylase 1/2 to the Viral Long Terminal Repeat MBio, 2021;0(0):e0079521. 2021 [PMID: 34281390] (Bioassay, Human) Bioassay Human
DP Strange, B Jiyarom, H Sadri-Arde, LH Cazares, TA Kenny, MD Ward, S Verma Paracrine IFN Response Limits ZIKV Infection in Human Sertoli Cells Frontiers in Microbiology, 2021;12(0):667146. 2021 [PMID: 34079533] (Bioassay, Human) Bioassay Human
X Yu, L Zhang, J Shen, Y Zhai, Q Jiang, M Yi, X Deng, Z Ruan, R Fang, Z Chen, X Ning, Z Jiang The STING phase-separator suppresses innate immune signalling Nature Cell Biology, 2021;23(4):330-340. 2021 [PMID: 33833429] (Bioassay, Human) Bioassay Human
R Tian, AJ Samelson, VV Rezelj, M Chen, GN Ramadoss, X Guo, AM Kain, QD Tran, SA Lim, I Lui, J Nunez, SJ Rockwood, N Liu, J Carlson-St, J Oki, T Maures, K Holden, JS Weissman, JA Wells, B Conklin, M Vignuzzi, M Kampmann BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2 bioRxiv : the preprint server for biology, 2021;0(0):. 2021 [PMID: 33501440] (Bioassay, Human) Bioassay Human
S Trump, S Lukassen, MS Anker, RL Chua, J Liebig, L Thürmann, VM Corman, M Binder, J Loske, C Klasa, T Krieger, BP Hennig, M Messingsch, F Pott, J Kazmierski, S Twardziok, JP Albrecht, J Eils, S Hadzibegov, A Lena, B Heidecker, T Bürgel, J Steinfeldt, C Goffinet, F Kurth, M Witzenrath, MT Völker, SD Müller, UG Liebert, N Ishaque, L Kaderali, LE Sander, C Drosten, S Laudi, R Eils, C Conrad, U Landmesser, I Lehmann Hypertension delays viral clearance and exacerbates airway hyperinflammation in patients with COVID-19 Nature Biotechnology, 2020;0(0):. 2020 [PMID: 33361824] (Bioassay, Human) Bioassay Human
Show All 29 Publications.

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Blogs on IFN-beta.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ...  Read full blog post.

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Bioinformatics

Gene Symbol IFNB1
Uniprot