TLR2 Antibody (TL2.1) [PE]

Flow Cytometry: TLR2 Antibody (TL2.1) [PE] [NBP2-24909] - Cell surface analysis of TLR2 on stable transfected cell line using this antibody at 1 ug/10^6 cells. Green represents isotype control ; red represents anti-TLR2 antibody.

Flow Cytometry: TLR2 Antibody (TL2.1) [PE] [NBP2-24909] - Cell surface analysis of TLR2 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control this antibody; red represents anti-TLR2 antibody.

• Reactivity: Hu, Mu, Rt, Ca, Rb
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
• Clone: TL2.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

TLR2 Antibody (TL2.1) [PE] Summary

• Additional Information: Clone TL2.1 was used by HLDA to establish CD designation.
• Immunogen: This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (Flo et al, 2000). The hybridoma supernatants were selected by flow cytometry.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow (Cell Surface)
  • Flow (Intracellular)
  • Flow Cytometry 1ul/1 million cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-5 ug/ml
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.
• Agonist:
  • Pam3CSK4, TLR1 and TLR2 Ligand NBP2-25297
  • SUCNR1/GPR91 Antibody NBP2-26239
  • MALP-2, TLR6 and TLR2 ligand NBP2-26219
• Antagonist:
  • TIRAP (TLR2 and TLR4) Inhibitor Peptide Set NBP2-29331

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 21273544).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for TLR2 Antibody (TL2.1) [PE]

• CD282 antigen
• CD282
• TIL4CD282
• TLR2
• toll/interleukin 1 receptor-like 4
• Toll/interleukin-1 receptor-like protein 4
• toll-like receptor 2

Background

Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR2 Antibody (NBP2-24909)(35)

Publications using NBP2-24909

• Chenwen X, Quanan J, Yee H, et al Efficacy of Rg1-Oil Adjuvant on Inducing Immune Responses against Bordetella bronchiseptica in Rabbits J Immunol Res 2021-02-12 [PMID: 33575363] (WB, Rabbit)
  
  Details:
  Citation using the PE version of this antibody.
• Akhter N, Madhoun A, Arefanian H et al Oxidative Stress Induces Expression of the Toll-Like Receptors (TLRs) 2 and 4 in the Human Peripheral Blood Mononuclear Cells: Implications for Metabolic Inflammation Cell. Cell Physiol Biochem. 2019-01-01 [PMID: 31162913] (FLOW, Human)
  
  Details:
  Citation using the PE version of this antibody.
• Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine)
  
  Details:
  Used the PE form of this antibody.
• Komine-Aizawa S, Hirohata N, Aizawa S, Abiko Y Porphyromonas gingivalis lipopolysaccharide inhibits trophoblast invasion in the presence of nicotine. Placenta. 2015-01-01 [PMID: 25468545] (FLOW, Human)
  
  Details:
  Citation using the PE version of this antibody.
• Green TL, Santos MF, Ejaeidi AA et al. Toll-like receptor (TLR) expression of immune system cells from metastatic breast cancer patients with circulating tumor cells. Exp Mol Pathol. 2014-08-01 [PMID: 24836676] (Flow-CS, Human)
  
  Details:
  Citation using the Azide Free format of this antibody.
• Verma R, Jung JH, Kim JY 1,25-Dihydroxyvitamin D3 up-regulates TLR10 while down-regulating TLR2, 4, and 5 in human monocyte THP-1. J Steroid Biochem Mol Biol. 2014-05-01 [PMID: 24373795] (FLOW, Human)
  
  Details:
  Citation using the PE version of this antibody.
• Klink M, Brzezinska M, Szulc I et al. Cholesterol oxidase is indispensable in the pathogenesis of Mycobacterium tuberculosis PLoS One. PLoS One. 2013-09-09 [PMID: 24039915] (FLOW, Human)
  
  Details:
  This citation used the PE version of this antibody.
• Tsai CC, Lin CR, Tsai HY et al. The immunologically active oligosaccharides isolated from wheatgrass modulate monocytes via Toll-like receptor-2 signaling J Biol Chem 2013-05-01 [PMID: 23629653]
• Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013-04-08 [PMID: 23593278]
  
  Details:
  Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
• Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013-01-01 [PMID: 23183897]
  
  Details:
  Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (
• Shimizu T, Kida Y, Kuwano K. Cytoadherence-dependent induction of inflammatory responses by Mycoplasma pneumoniae. Immunology. 2011-05-01 [PMID: 21320122] (WB)
  
  Details:
  TLR2/CD282 (IMG-416A). WB: THP-1 cells treated with living or heat killed M. pneumoniae, Fig 3.
• Dasgupta G, Chentoufi AA, You S et al. Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses. Invest Ophthalmol Vis Sci. 2011-05-17 [PMID: 21273544] (Flow Cytometry Control, Rabbit)
  
  Details:
  Products cited: Flow (intracellular), Fig 1A ( CD4+CD25+ regulatory T cells purified from rabbit conjunctiva) & Fig 2A (CD11b+ cells isolated from rabbit conjunctiva): TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315D), TLR4/CD284 (IMG-417D), TLR8/CD288 (IMG
• Oberg HH, Ly TT, Ussat S et al. Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands. J Immunol. 2010-05-01 [PMID: 20363971] (Flow Cytometry Control)
  
  Details:
  Flow(intracellular) cytometry: TLR2/CD282 PE (IMG-416D) and TLR6/CD286 (IMG-304A). T cells and Tregs were separated from freshly isolated human PBMCs, Fig S3.
• Ding J, Rapista A, Teleshova N et al. Neisseria gonorrhoeae enhances HIV-1 infection of primary resting CD4+ T cells through TLR2 activation. J Immunol. 2010-03-15 [PMID: 20147631] (Flow-CS, Human)
  
  Details:
  TLR2/CD282 (IMG-416C). Flow (Cell surface), Fig 6D (human CD4+T cells).
• Rydberg C, Mansson A, Uddman R et al. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology. 2009-09-01 [PMID: 19740321] (Flow Cytometry Control)
  
  Details:
  TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315A), TLR3/CD283 (IMG-315D), TLR5 PE (IMG-663D), TLR5 (IMG-663). 1. IHC(paraffin): Human head and neck squamous (HNSCC) cell carcinomas showing keratinized cell carcinoma from the larynx stained with TLR2 (IMG-416A) and TLR5 antibodies (IMG-663A), Fig 1A, 1B, & 1C. 2. Flow (intracellular): TLR2 PE (IMG-416D), TLR3 PE (IMG-315D), and TLR5 PE (IMG-663D) antibodies were used in human bronchial epithelial (NL-20) and human pharyngeal squamous (Detroit-562) carcinoma cell line, Fig 2C.
• Yang X, Fullerton DA, Su X et al Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2. J Am Coll Cardiol. 2009-02-10 [PMID: 19195606] (WB, Human)
  
  Details:
  Citation using the Azide Free version of this antibody.
• Ji S, Shin JE, Kim YS et al. Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. Infect Immun 2009-03-01 [PMID: 19103770] (Flow-CS)
• Guzy C, Paclik D, Schirbel A et al. The probiotic Escherichia coli strain Nissle 1917 induces gammadelta T cell apoptosis via caspase- and FasL-dependent pathways. Int Immunol. 2008-07-01 [PMID: 18448456]
  
  Details:
  Antibodies cited [Flow (Cell Surface) (human PBMCs), Fig. 5]: 1. TLR2 FITC [IMG-416C] 2. TLR4 FITC [IMG-417C].
• Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008-07-15 [PMID: 18439687]
  
  Details:
  Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.
• Prabha C, Rajashree P, Sulochana DD. TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid. Immunol Lett. 2008-04-15 [PMID: 18295348]
  
  Details:
  TLR2- FITC (IMG-416C): Flow (cell surface): Figs. 1A, B (human CD4+T cells, CD8+T cells, B cells, CD16+56+ cells and monocytes); 2(CD4+T cells); 4A, B (human Treg cells). Flow (intracellular): Fig. 3A, B (CD4+T cells) 2. TLR4- FITC (IMG-417C).Flow (cell surface): Figs. 1B, C (human CD4+T cells, CD8+T cells, B cells, CD16+56+ cells and monocytes); 2(CD4+T cells); 4A, B (human Treg cells). Flow (intracellular): Fig. 3A, B (CD4+T cells).
• Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008-03-01 [PMID: 18292566]
  
  Details:
  Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
• Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440]
  
  Details:
  Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
• Menzies BE, Kenoyer A. Signal transduction and nuclear responses in Staphylococcus aureus-induced expression of human beta-defensin 3 in skin keratinocytes. Infect Immun. 2006-12-01 [PMID: 16954397] (FLOW, Human)
  
  Details:
  TLR2 [IMG-416E] 1. FA (human keratinocytes), Fig. 2.
• Xu S, Koldovsky U, Xu M et al High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production. Surgery. 2006-08-01 [PMID: 16904966]
  
  Details:
  Citation using the Azide Free version of this antibody.
• Shimizu T, Kida Y, Kuwano K. A dipalmitoylated lipoprotein from Mycoplasma pneumoniae activates NF-kappa B through TLR1, TLR2, and TLR6. J Immunol. 2005-10-01 [PMID: 16177110]
  
  Details:
  TLR2 [IMG-416E] 1. FA (THP-1 cells), Fig. 2.
• Srivastava MD, Srivastava BI. Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line. Leuk Res. 2005-07-01 [PMID: 15927677] (ICC/IF)
  
  Details:
  IF/ICC in SRIK-NKL cells: TLR2 (IMG416), TLR4 (IMG-417), TLR7 (IMG-581), and TLR9 (IMG-305).
• Bazzocchi C, Mortarino M, Comazzi S et al. Expression and function of Toll-like receptor 2 in canine blood phagocytes. Vet Immunol Immunopathol. 2005-03-10 [PMID: 15661327] (FLOW, Canine)
  
  Details:
  TLR2 [IMG-416A] 1. Flow (whole blood cells from dogs), Fig. 3.
• Zhong F, Cao W, Chan E et al. Deviation from major codons in the Toll-like receptor genes is associated with low Toll-like receptor expression Immunology 2005-01-01 [PMID: 15606798] (Flow Cytometry Control)
  
  Details:
  This citation used the FITC version of this antibody.
• Gibson FC 3rd, Hong C, Chou HH et al. Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice. Circulation. 2004-06-08 [PMID: 15123526] (FLOW, IHC-P)
  
  Details:
  TLR2 [IMG-416]: 1. IHC:P (aortic tissue), Fig. 2b 2. Flow (Cell Surface) (P gingivalis, mutant P gingivalis), Figs. 3a, 3b.
• Mempel M, Voelcker V, Kollisch G et al. Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent. J Invest Dermatol. 2003-12-01 [PMID: 14675188]
  
  Details:
  TLR2 (IMG-416) 2. TLR4 (IMG-417) [IF/ICC, Fig.2A and 2D (human keratinocytes)].
• Lotz Sonja, Aga Eresso, Wilde Inga et al. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 2004-03-01 [PMID: 14673018] (FLOW)
• Medvedev AE, Lentschat A, Wahl LM et al. Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells. J Immunol. 2002-11-01 [PMID: 12391239] (WB, FLOW, Human)
  
  Details:
  TLR2 (IMG-416A): 1. WB (human monocytes), Fig. 3 2. Flow (Cell Surface) (human monocytes), Fig. 4.
• Flo TH, Halaas O, Torp S et al. Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol. 2001-03-01 [PMID: 11261796]
  
  Details:
  Antibodies cited: TLR2 (IMG-416): 1. Flow (Cell Surface): Figs 1 (whole blood), 5 (granulocytes), and 6 (monocytes) 2. IP: Fig 2 (monocytes) 3. IF/ICC: Fig 3 (monocyte-derived macrophages).
• Faure E, Thomas L, Xu H et al. Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation. J Immunol 2001-02-01 [PMID: 11160251] (IHC-P, WB)
  
  Details:
  This citation used the Biotin version of this antibody.
• Flo TH, Halaas O, Lien E et al. Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol. 2000-02-15 [PMID: 10657659]
  
  Details:
  TLR2 (IMG-416): 1. Generation of the IMG-416 TL2.1 antibody clone, recognizing a TLR2-associated epitope (Materials and Methods).

Review for TLR2 Antibody (NBP2-24909) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 12/29/2014

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 24039915
• Species: Human

Bioinformatics

• Gene Symbol: TLR2