p62/SQSTM1 Antibody


Western Blot: p62/SQSTM1 Antibody [NBP1-49954] - Detection of Human and Mouse Sequestosome-1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for ...read more
Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody [NBP1-49954] - Human breast carcinoma.
Simple Western: p62/SQSTM1 Antibody [NBP1-49954] - Simple Western lane view shows a specific band for p62/SQSTM1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, Mu, Bovine, Canine, Chicken, Equine, Guinea Pig, Primate, Porcine, Rabbit, Rat, Sheep, Chinese Hamster, Turkey, Rhesus MacaqueSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P, IP
1 mg/ml

Order Details

p62/SQSTM1 Antibody Summary

The immunogen for this product maps to a region between residue 325 and 375 of human Sequestosome-1 using the numbering given in entry NP_003891.1 (GeneID 8878).
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%), Guinea Pig (100%), Rabbit (100%), Rhesus Macaque (100%), Chicken (100%), Canine (100%), Sheep (100%), Primate (100%), Turkey (100%), Equine (100%), Chinese Hamster (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:2000-1:10000
  • Simple Western 1:40
  • Immunohistochemistry 1:500 to 1:2000
  • Immunohistochemistry-Paraffin 1:500 to 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publication using NBP1-49954.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee, White-tufted-ear marmoset, Naked mole rat, and African elephant.

Alternate Names for p62/SQSTM1 Antibody

  • A170
  • EBI3-associated protein of 60 kDa
  • EBI3-associated protein p60
  • ORCA
  • OSIL
  • oxidative stress induced like
  • p60PDB3
  • p62
  • p62B
  • Paget disease of bone 3
  • PDB3
  • phosphotyrosine independent ligand for the Lck SH2 domain p62
  • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
  • Sequestosome 1
  • sequestosome-1
  • SQSTM1
  • Ubiquitin-binding protein p62
  • ZIP3


SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. More recently, a role for SQSTM1/p62 in macroautophagic removal of intracellular protein aggregates has also been proposed. Studies involving the cellular depletion of SQSTM1/p62 have indicated a need for its association with LC3 and the aggregate proteins in order to facilitate correct formation of the autophagosome. Defects in SQSTM1/p62 are a cause of Paget disease of bone which is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p62/SQSTM1 Antibody (NBP1-49954)(1)

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WB Video Protocol

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Secondary Antibodies


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Blogs on p62/SQSTM1.

ATG4C - A regulator of the early steps of autophagosome assembly
Autophagy is an important cellular process that maintains homeostasis by degrading and recycling damaged proteins and organelles. Autophagy receptors, such as p62/SQSTM1, recognize these intracellular cargo and mediate their engulfment by the doubl...  Read full blog post.

p62/SQSTM1 - targeting ubiquitinated proteins for autophagic degradation
During autophagy ubiquitinated cargo or substrates are engulfed in a double-membrane autophagosome and transported to the lysosome for degradation. This process is important for maintaining cellular homeostasis and for degrading damaged organelles...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

p62/SQSTM1 (sequestosome 1)
p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adaptor protein. SQSTM1 functions as a signaling hub for various signal transduction pathways involved in apoptosis, cell differentiation, apoptosis, immune response, and K+ channel reg...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol SQSTM1

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