| Reactivity | Hu, Mu, Rt, Rt, Po, Bt, Bv, Fi, Xp, ZeSpecies Glossary |
| Applications | Flow, KO |
| Clone | 1251A |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | PE |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100). [UniProt# Q9GZQ8]. |
| Localization | LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes. |
| Marker | Autophagosomes |
| Specificity | Specific for LC3B. |
| Predicted Species | Rat (94%), Porcine (100%), Bovine (100%), Zebrafish (100%), Fish (100%), Bat (100%), Xenopus (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MAP1LC3B |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
![LC3B Antibody (1251A) [PE]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "LC3B Antibody (1251A) [PE]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 405 Labeled | NBP2-46892AF405 | |
| Alexa Fluor 488 Labeled | NBP2-46892AF488 | |
| Alexa Fluor 647 Labeled | NBP2-46892AF647 | |
| Alexa Fluor 700 Labeled | NBP2-46892AF700 | |
| Allophycocyanin Labeled | NBP2-46892APC | |
| Biotin Labeled | NBP2-46892B | |
| DyLight 350 Labeled | NBP2-46892UV | |
| DyLight 405 Labeled | NBP2-46892V | |
| DyLight 488 Labeled | NBP2-46892G | |
| DyLight 550 Labeled | NBP2-46892R | |
| DyLight 650 Labeled | NBP2-46892C | |
| DyLight 680 Labeled | NBP2-46892FR | |
| DyLight 755 Labeled | NBP2-46892IR | |
| HRP Labeled | NBP2-46892H | |
| PerCP Labeled | NBP2-46892PCP |
Diseases for LC3B Antibody (NBP2-46892PE)Discover more about diseases related to LC3B Antibody (NBP2-46892PE).
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PTMs for LC3B Antibody (NBP2-46892PE)Learn more about PTMs related to LC3B Antibody (NBP2-46892PE).
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Crosstalk Between Oxidative Stress and Autophagy By Christina Towers, PhD. Role of Reactive Species in Cellular FunctionOxidative stress is a byproduct of an imbalance between oxidants and antioxidants present in the cell resulting in dysfunctional redox signali... Read full blog post. |
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Autophagy inhibition in pediatrics: One physician-scientist’s brave decision By Christina Towers, PhD. The current time from when a discovery is first made on the bench to when that discovery might translate into an approved therapy in cancer patients is an astounding 10-15 years. Scientists... Read full blog post. |
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Cleaner gone bad: Autophagy regulates motor neuron loss in spinal muscular atrophy By Jamshed Arslan Pharm.D. Neuromuscular disorders affect the peripheral nervous system and muscles. Spinal muscular atrophy (SMA) is one such incurable disease in which muscles fail to receive signals from the spi... Read full blog post. |
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Brain size matters: MTOR regulates autophagy and number of cortical interneurons By Jamshed Arslan Pharm.D. Interneurons transmit impulses between other neurons, in part, to facilitate the birth of neurons. Cortical interneurons themselves arise from the progenitors in the ventral telencephalon,... Read full blog post. |
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Why LC3B Antibodies Make Ideal Autophagosomes Membrane Markers The human form of microtubule-associated protein light chain 3 (LC3) is expressed as 3 splice variants; LC3A, LC3B and LC3C (He et al., 2003). LC3B is a subunit of the MAP1A and MAP1B microtubule-binding pr... Read full blog post. |
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LC3B: From Autophagy to Cancer LC3B is subunit component of the LC3 autophagy biomarker associated with microtubule-associated proteins MAP1A and MAP1B and one of the best characterized markers to date. In resting state it is cytosolic, but upon activation, is lapidated and becomes... Read full blog post. |
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LC3B Empowers Protein Quality Control by Autophagy LC3B also known as microtubule-associated protein 1 light chain 3 beta (MAP1LC3B) is an autophagy gene that contributes appreciably to protein degradation. Autophagy is a highly synchronized and a dynamic catabolic degradation activity that plays an e... Read full blog post. |
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Analyzing LC3 in Western blot Microtubule-associated protein light chain 3 (LC3) is considered one of the definitive markers of autophagy, and its use is widespread in labs throughout the world. Despite its popularity, there are several considerations when employing LC3 antibodies... Read full blog post. |
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Marking the Autophagosome: the LC3 Antibody MAP1LC3 (shortened to LC3 in our antibody catalog) is one of four mammalian homologues of autophagy-related protein 8 (Atg8). It has been identified as a light chain subunit of the microtubule-associated proteins MAP1A/MAP1B. A modified form of LC3, L... Read full blog post. |
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Breakdown: Interpreting LC3 Antibody WB Results In rodents, MAPLC3 (Microtubule-associated protein 1 light chain 3) is expressed in the renal visceral epithelial cells, or podocytes. LC3 antibody analysis has shown the protein accumulates in its membrane-bound form of LC3II, following conversion fr... Read full blog post. |
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![Western Blot: LC3A Antibody [NB100-2331] - WB analysis of heart tissue lysates from mice which were subjected or not to 48 hours of starvation. The signal was developed using ECL method and this LC3 antibody was found to detect both forms of LC3, i.e. LC3-I and LC3-II. As expected, the levels of LC3-II form were higher in the heart tissue lysates from starved mice. The bottom image is of the coomassie blue staining.](http://images.novusbio.com/fullsize2/LC3A-Antibody-Western-Blot-NB100-2331-img0029.jpg)
![Western Blot: LC3A Antibody [NB100-2331] - Detection of HRP conjugated autophagic LC3 in mouse ES cell lysate. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.](http://images.novusbio.com/fullsize2/LC3A-Antibody-Western-Blot-NB100-2331-img0028.jpg)
![Western Blot: LC3A Antibody [NB100-2331] - Analysis in human lysates.](http://images.novusbio.com/fullsize2/LC3A-Antibody-Western-Blot-NB100-2331-img0027.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).](http://images.novusbio.com/fullsize2/p62-SQSTM1-Antibody-(2C11)-Western-Blot-H00008878-M01-img0012.jpg)
![Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of p62 in p62 WT and KO mouse hepatocytes and HepG2 cell lysate using anti-p62/SQSTM1 antibody clone 2C11. Sample lane1: p62 +/- mouse hepatocytes, lane 2: p62 -/- mouse hepatocytes, lane3: Wt mouse hepatocytes, lane 4: HepG2 cells. This image is from a verified customer product review.](http://images.novusbio.com/fullsize2/p62-SQSTM1-Antibody-(2C11)-Western-Blot-H00008878-M01-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - ICC-IF analysis of SQSTM1 protein in HeLa cell using SQSTM1/p62 antibody (clone 2C11) at 10 ug/ml concentration.](http://images.novusbio.com/fullsize2/p62-SQSTM1-Antibody-(2C11)-Immunocytochemistry-Immunofluorescence-H00008878-M01-img0008.jpg)
![Western Blot: Beclin 1 Antibody [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - WB analysis of Belclin1. Lane 1 human brain and Lane 2 mouse brain.](http://images.novusbio.com/fullsize2/Beclin-1-Antibody-Western-Blot-NB500-249-img0011.jpg)
![Simple Western: Beclin 1 Antibody [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Simple Western lane view shows a specific band for Beclin1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Beclin-1-Antibody-Simple-Western-NB500-249-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Beclin 1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/Beclin-1-Antibody-Immunocytochemistry-Immunofluorescence-NB500-249-img0013.jpg)
![Western Blot: GABARAPL2 Antibody [NBP1-88883] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/GABARAPL2 Antibody-Western Blot-NBP1-88883-img0005.jpg)
![Immunohistochemistry-Paraffin: GABARAPL2 Antibody [NBP1-88883] - Staining of human cerebellum shows distinct cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize2/GABARAPL2-Antibody-Immunohistochemistry-Paraffin-NBP1-88883-img0002.jpg)
![Western Blot: GABARAPL1 Antibody [NBP1-55202] - Hela Whole Cell lysates, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize2/GABARAPL1 Antibody-Western Blot-NBP1-55202-img0005.jpg)
![Immunohistochemistry-Paraffin: GABARAPL1 Antibody [NBP1-55202] - Human Brain lysate tissue at an Antibody concentration of 5.0 ug/ml using anti-GABARAPL1 Antibody (ARP55398_P050).](http://images.novusbio.com/fullsize2/GABARAPL1-Antibody-Immunohistochemistry-Paraffin-NBP1-55202-img0004.jpg)
![Western Blot: GABARAPL1 Antibody [NBP1-55202] - Transfected 293T tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize2/GABARAPL1-Antibody-Western-Blot-NBP1-55202-img0001.jpg)
![Western Blot: GTF2H1 Antibody [NBP2-38556] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize2/GTF2H1-Antibody-Western-Blot-NBP2-38556-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody [NBP2-38556] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/GTF2H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38556-img0004.jpg)
![Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize2/GTF2H1-Antibody-Immunohistochemistry-Paraffin-NBP2-38556-img0010.jpg)
![Western Blot: NUP62 Antibody [NBP1-31381] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/NUP62-Antibody-Western-Blot-NBP1-31381-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleoporin p62 Antibody [NBP1-31381] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody diluted at 1:750. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize2/Nucleoporin-p62-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31381-img0012.jpg)
![Immunohistochemistry-Paraffin: Nucleoporin p62 Antibody [NBP1-31381] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Nucleoporin p62 Antibody-Immunohistochemistry-Paraffin-NBP1-31381-img0008.jpg)
![Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mouse mAb against Raw264.7 (1) and NIH3T3 (2) cell lysate.](http://images.novusbio.com/fullsize2/dynactin-4-Antibody-(3G9D7)-Western-Blot-NBP2-37399-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunofluorescence analysis of HepG2 cells using DCTN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize2/dynactin-4-Antibody-(3G9D7)-Immunocytochemistry-Immunofluorescence-NBP2-37399-img0005.jpg)
![Immunohistochemistry: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DCTN4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/dynactin-4-Antibody-(3G9D7)-Immunohistochemistry-NBP2-37399-img0008.jpg)
![Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2 Antibody (3F9)-Western Blot-NBP2-02627-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2.](http://images.novusbio.com/fullsize/IGF2BP2 Antibody (3F9)-Immunocytochemistry-Immunofluorescence-NBP2-02627-img0009.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Human liver tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2 Antibody (3F9)-Immunohistochemistry-Paraffin-NBP2-02627-img0008.jpg)
![Western Blot: SAM68 Antibody [NBP2-38645] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-KHDRBS1 antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize2/SAM68-Antibody-Western-Blot-NBP2-38645-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SAM68 Antibody [NBP2-38645] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize2/SAM68-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38645-img0003.jpg)
![Immunohistochemistry-Paraffin: SAM68 Antibody [NBP2-38645] - Staining in human endometrium and skeletal muscle tissues using anti-KHDRBS1 antibody. Corresponding KHDRBS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/SAM68-Antibody-Immunohistochemistry-Paraffin-NBP2-38645-img0006.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Detection of a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize2/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0004.jpg)
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize2/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-NB600-607-img0003.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. Caspase-3 activation is determined by cleavage of procaspase-3.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0032.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Western Blot Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0031.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0037.jpg)
![Western Blot: ATG5 Antibody [NB110-53818] - Total protein from Human HeLa and A431 and Mouse MEF cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/ATG5-Antibody-Western-Blot-NB110-53818-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NB110-53818] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ATG5 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NB110-53818-img0016.jpg)
![Immunohistochemistry: ATG5 Antibody [NB110-53818] - Staining of human liver hepatocytes at 2.5ug/ml. 40X magnification.](http://images.novusbio.com/fullsize2/ATG5-Antibody-Immunohistochemistry-NB110-53818-img0010.jpg)
![Western Blot: ATG4B Antibody [NBP2-24709] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24709 at 2 ug/ml.](http://images.novusbio.com/fullsize2/ATG4B-Antibody-Western-Blot-NBP2-24709-img0001.jpg)
![Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-24709] - Analysis of human heart using NBP2-24709 at 10 ug/ml.](http://images.novusbio.com/fullsize2/ATG4B-Antibody-Immunohistochemistry-Paraffin-NBP2-24709-img0002.jpg)
![Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.](http://images.novusbio.com/fullsize2/GADD153-CHOP-Antibody-(9C8)-Western-Blot-NB600-1335-img0011.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - IHC analysis of a formalin fixed and paraffin embedded (FFPE) tissue section of mouse brain using 1:100 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.](http://images.novusbio.com/fullsize2/GADD153-CHOP-Antibody-(9C8)-Immunohistochemistry-Paraffin-NB600-1335-img0010.jpg)
![Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Western blot analysis of GADD153/CHOP expression in HeLa cells treated with 2.5ug/ml tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).](http://images.novusbio.com/fullsize2/GADD153-CHOP-Antibody-(9C8)-Western-Blot-NB600-1335-img0001.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with scrambled control siRNA (lane 1) or Bcl-2 siRNA (lane2). Image from verified customer review.](http://images.novusbio.com/fullsize2/Bcl-2-Antibody-Western-Blot-NB100-56098-img0004.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - WB analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).](http://images.novusbio.com/fullsize2/Bcl-2-Antibody-Western-Blot-NB100-56098-img0007.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:1000 dilution of Bcl-2 antibody (NB100-56098) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of BCL2 in the cancer cells with relatively intense signal in their nuclear membranes. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize2/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56098-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LC3C Antibody [NBP2-57604] - Staining of human cell line RH-30 shows localization to cytoplasmic bodies.](http://images.novusbio.com/fullsize2/LC3C Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57604-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [PE] [NBP2-24983] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details].](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-[PE]-Flow-Cytometry-NBP2-24983-img0003.jpg "Flow Cytometry: Rabbit IgG Isotype Control [PE] [NBP2-24983] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details].")
![Knockout Validated: LC3B Antibody (1251A) [NBP2-46892] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF membrane was probed with 2.0 ug/mLof Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in theknockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/LC3B-Antibody-(1251A)-Knockout-Validated-NBP2-46892-img0028.jpg "Knockout Validated: LC3B Antibody (1251A) [NBP2-46892] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF membrane was probed with 2.0 ug/mLof Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in theknockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.")
![SDS-Page: Recombinant Human Aldolase C Protein [NBP2-61172] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/images2/Recombinant Human Aldolase C Protein-SDS-Page-NBP2-61172-img0001.jpg "SDS-Page: Recombinant Human Aldolase C Protein [NBP2-61172] - 15 % SDS-PAGE (3ug)")