Rabbit IgG Isotype Control [PE]

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Flow Cytometry: Rabbit IgG Isotype Control [PE] [NBP2-24983] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose ...read more
Flow Cytometry: Rabbit IgG Isotype Control [PE] [NBP2-24983] - PBMCs fixed and permeabilized using the Novus intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or rabbit IgG-PE conjugate ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
PE

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Rabbit IgG Isotype Control [PE] Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ul/1 million cells
Application Notes
Use the isotype control at the same concentration as the experimental antiobdy. Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24983 in the following application:

Publications
Read Publications using NBP2-24983.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Affinity purified

Alternate Names for Rabbit IgG Isotype Control [PE]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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Publications for IgG Isotype Control (NBP2-24983)(4)

We have publications tested in 2 applications: FLOW, Flow-CS.


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Showing Publications 1 - 4 of 4.
Publications using NBP2-24983 Applications Species
Zimmermann M, Aguilera FB, Castellucci M et al. Chromatin remodelling and autocrine TNFalpha are required for optimal interleukin-6 expression in activated human neutrophils Nat Commun. 2015 Jan 24 [PMID: 25616107] (FLOW)

Details:
Rabbit IgG Isotype Control - PE conjugated / Imgenex: 20304D used for FLOW application as isotype control with TLR7 antibody - PE conjugated (NBP2-24761) on human neutrophils - 4% paraformaldehyde fixation, permeabilization with solution containing 0.5% saponin and 5% human, rabbit IgG Isotype control-PE as well as the PE-conjugated TLR7 primary antibody used at 1:30 dilution, samples analyzed with seven-colour MACSQuant Analyzer (Supplementary Figure 2).
FLOW
Shimazu T, Villena J, Tohno M et al. Immunobiotic Lactobacillus jensenii elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the Toll-like receptor signaling pathway. Infect Immun. 2012 Jan [PMID: 22083706] (Flow-CS)

Details:
Cat no 20304E (normal rabbit IgG isotype control): Flow (cell surface), Fig 4C (porcine intestinal epitheliocyte cell line).
Flow-CS
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. 2010 Mar [PMID: 19922426]
Patole PS, Pawar RD, Lech M et al. Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice. Nephrol Dial Transplant. 2006 Nov [PMID: 16954173]

Details:
Antibodies cited: For IHC (frozen) on mouse kidney sections (Fig. 5): TLR3 (IMG-516), TLR7 (IMG-581A), TLR9 (IMG-431) and Rabbit IgG isotype control antibody (20304). For Flow (Intacellular) and Flow (Cell surface) on J774 mouse macrophage-like cell line

Review for IgG Isotype Control (NBP2-24983) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP2-24983:
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Images Ratings Applications Species Date Details
Flow Cytometry IgG NBP2-24983
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reviewed by:
Anonymous
Flow Other 02/26/2015
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Summary

ApplicationFlow Cytometry
Sample TestedMurine CD4+ T cells
SpeciesOther
LotA-1-PE

Blocking

Blocking DetailsNone

Primary Anitbody

Dilution Ratio1:100 dilution in 1X Permeabilization buffer (Foxp3 Staining Buffer Set from eBiosciences), 30 minutes incubation at 21°C

Details

Detection NotesLSR II Flow Cytometer (BD); Controls - Fluorescence Minus One/FMO and Glut1 stained Mouse CD4+ T Cells (NB110-39113PE, Orange)
Fixation DetailsSee Additional Comments
Permeabilization DetailsCells fixed and permeabilized with Foxp3 Staining Buffer set (eBioSciences #00-5523-00) for intracellular staining
Wash DescriptionOne wash with 1X Permeabilization buffer (eBiosciences) and one wash with FACS buffer (1x PBS, 1% BSA, 0.1% sodium azide)

Comments

CommentsMurine spleens were harvested and CD4+ T cells were isolated via negative selection. CD4+ T cells were stimulated with anti-CD3/CD28 beads and insulin (1μg/mL) for 5 days in culture media with additional glucose provided. Cells were washed and beads removed prior to staining. Cells were stained for surface antigens, washed, then fixed and permeabilized (via Foxp3 Staining Buffer set (eBioSciences #00-5523-00)) for intracellular staining (Novus informed me that the epitope is intracellular for this antibody). Cells were washed again before being ran on LSR II Flow Cytometer. Data was analyzed via FlowJo (Tree Star). Cells were gated, in order, on lymphocytes, single cells, live cells, and CD4+ cells. Test samples were compared to both isotype control, as well as FMO control.

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FAQs for IgG Isotype Control (NBP2-24983) (0)

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CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Recent Reviews

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Anonymous
02/26/2015
Application: Flow
Species: Other