Rabbit IgG Isotype Control [PE]

Images

 
Flow Cytometry: Rabbit IgG Isotype Control [PE] [NBP2-24983] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose ...read more
Flow Cytometry: Rabbit IgG Isotype Control [PE] [NBP2-24983] - PBMCs fixed and permeabilized using the Novus intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or rabbit IgG-PE conjugate ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
PE

Order Details

Rabbit IgG Isotype Control [PE] Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGHG1
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ul/1 million cells
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24983 in the following application:

Publications
Read Publications using NBP2-24983.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Affinity purified

Alternate Names for Rabbit IgG Isotype Control [PE]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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Publications for IgG Isotype Control (NBP2-24983)(6)

We have publications tested in 2 applications: FLOW, Flow-CS.


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Showing Publications 1 - 6 of 6.
Publications using NBP2-24983 Applications Species
Kumagai S, Koyama S, Itahashi K Et al. Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments Cancer Cell 2022-01-29 [PMID: 35090594]

Details:
Citation using the PE version of this antibody.
Kumagai S, Togashi Y, Sakai C et al. An Oncogenic Alteration Creates a Microenvironment that Promotes Tumor Progression by Conferring a Metabolic Advantage to Regulatory T Cells Immunity 2020-07-14 [PMID: 32640259]

Details:
Citation using the PE format of this antibody.
Zimmermann M, Aguilera FB, Castellucci M et al. Chromatin remodelling and autocrine TNFalpha are required for optimal interleukin-6 expression in activated human neutrophils. Nat Commun. [PMID: 25616107] (FLOW)

Details:
Citation using the PE form of this antibody.
FLOW
Shimazu T, Villena J, Tohno M et al. Immunobiotic Lactobacillus jensenii elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the Toll-like receptor signaling pathway. Infect Immun. 2012-01-01 [PMID: 22083706] (Flow-CS)

Details:
Cat no 20304E (normal rabbit IgG isotype control): Flow (cell surface), Fig 4C (porcine intestinal epitheliocyte cell line).
Flow-CS
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. [PMID: 19922426]

Details:
Citation using the PE/Cy5 form of this antibody.
Patole PS, Pawar RD, Lech M et al. Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice. Nephrol Dial Transplant. 2006-11-01 [PMID: 16954173]

Details:
For IHC (frozen) on mouse kidney sections (Fig. 5): TLR3 (IMG-516), TLR7 (IMG-581A), TLR9 (IMG-431) and Rabbit IgG isotype control antibody (20304). For Flow (Intacellular) and Flow (Cell surface) on J774 mouse macrophage-like cell line (Fig 6B): TLR7 (IMG-581A), TLR9 (IMG-431) and Rabbit IG isotype control antibody (20304).

Review for IgG Isotype Control (NBP2-24983) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP2-24983:
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Images Ratings Applications Species Date Details
Flow Cytometry IgG NBP2-24983
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5
reviewed by:
Verified Customer
Flow Other 02/26/2015
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Summary

ApplicationFlow Cytometry
Sample TestedMurine CD4+ T cells
SpeciesOther
LotA-1-PE

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IgG Isotype Control (NBP2-24983) (0)

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Additional IgG Products

Research Areas for IgG Isotype Control (NBP2-24983)

Find related products by research area.

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

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Verified Customer
02/26/2015
Application: Flow
Species: Other

Bioinformatics

Gene Symbol IGHG1