iNOS Antibody

Images

 
Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.

Product Details

Summary
Reactivity Mu, Rt, BvSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

iNOS Antibody Summary

Immunogen
Synthetic peptide corresponding to aa 1131-1144 of mouse iNOS.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOS2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunoprecipitation 1:10-1:500
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-97471 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for iNOS Antibody

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS
  • NOS, type II
  • NOS2
  • NOS2A
  • NOS2ANOS type II

Background

The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for iNOS Antibody (NBP1-97471)(1)

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 1 application: WB.


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WB
(1)
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Bovine
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for iNOS Antibody (NBP1-97471). (Showing 1 - 4 of 4 FAQ).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. Would you please confirm the concentration of the current lot of this Ab: iNOS Antibody (NBP1-97471) - 0.05mg
    • The antibody is supplied at 1mg/ml.
  3. What is the concentration of this product?
    • The concentration for this product (lot 01061105) is 1.0mg/ml.
  4. I used NBP1-97471 antibody to run WB on cow mammary epithelial cells treated by LPS. I used 60ug/lane, 10% gel, and I found there was no specific band. Would you please give some advice?
    • I think you can try running slightly less protein/lane. We usually use 20-30ug, so you can try 30-40ug instead of 60ug. The 10% gel should be fine, but if you want to separate the higher weights more, you can try using a lower percentage gel.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for iNOS Antibody (NBP1-97471)

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Blogs on iNOS.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Bioinformatics

Gene Symbol NOS2
Entrez