ICC/IF reactivity reported in scientific literature (PMID:27613149). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29295997). Use in Immunohistochemistry-P reactvity per customer review.
Read 1 Review rated 5 using NBP1-33780 in the following applications:
This antibody recognize nNOS (neuronal nitric oxide synthase) also. Porcine reactivity per customer review.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20 mM Tris-HCl, pH 8.0, 10 mg/ml BSA
0.05% Sodium Azide
Protein A or G purified
The 7mL size that this product is available in is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Alternate Names for iNOS Antibody (K13-A)
HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
Inducible NO synthase
nitric oxide synthase 2, inducible
nitric oxide synthase 2A (inducible, hepatocytes)
nitric oxide synthase, inducible
nitric oxide synthase, macrophage
NOS, type II
NOS2ANOS type II
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for iNOS Antibody (NBP1-33780). (Showing 1 - 3 of 3 FAQs).
We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
Hi, I would like to know if this antibody (NBP1-33780) works on mouse tissue in Immunohistochemistry. Did you test the mouse cross-reactivity on IHC ?
At this time we have only tested NBP1-33780 on human tissues in IHC, but we do expect it to work on mouse tissues in IHC based on sequence homology.
What is the concentration of this product?
The concentration of the lot number DB003-02-5B is 4.9 mg/ml
iNOS: The Nitric Oxide Boss Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti... Read full blog post.
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