iNOS Antibody (K13-A)

Images

 
Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um ...read more
Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Lung adenocarcinoma tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human ...read more
Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Inflamed porcine intestine. Antibody dilution 1:200, overnight 4C. Secondary anti-rabbit Alexa 488 (1:1000) 2 hr incubation. Image from verified ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
K13-A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

iNOS Antibody (K13-A) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant monoclonal iNOS Antibody (K13-A) was made to a peptide derived from human iNOS sequence. iNOS Antibody (K13-A) Antibody recognizes the epitope between Ser1118 - Gly1129.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NOS2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • ELISA 1:20000-1:50000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Immunoprecipitation
Application Notes
Use in ICC/IF reported in scientific literature (PMID:27613149). Use in IHC-Fr reported in scientific literature (PMID: 29295997). Use in WB reported in scientific literature (PMID: 30391674).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-33780 in the following applications:

Publications
Read Publications using
NBP1-33780 in the following applications:

Reactivity Notes

This antibody also recognizes nNOS (neuronal nitric oxide synthase). Porcine reactivity reported per verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl, pH 8.0, 10 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The 7mL size that this product is available in is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Alternate Names for iNOS Antibody (K13-A)

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS type II
  • NOS
  • NOS2
  • NOS2A
  • Peptidyl-cysteine S-nitrosylase NOS2

Background

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3.Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for iNOS Antibody (NBP1-33780)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
KD
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(1)
All Species

Review for iNOS Antibody (NBP1-33780) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Porcine.

Reviews using NBP1-33780:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Porcine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin iNOS NBP1-33780
Enlarge
reviewed by:
Sandra Lourenssen
IHC-P Porcine 04/18/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedparaffin sections of inflamed intestine
SpeciesPorcine
LotDB003-02-06B

Comments

CommentsAntibody dilution 1:200, overnight 4C. Secondary anti-rabbit Alexa 488 (1:1000) 2 hr incubation.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for iNOS Antibody (NBP1-33780). (Showing 1 - 3 of 3 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. Hi, I would like to know if this antibody (NBP1-33780) works on mouse tissue in Immunohistochemistry. Did you test the mouse cross-reactivity on IHC ?
    • At this time we have only tested NBP1-33780 on human tissues in IHC, but we do expect it to work on mouse tissues in IHC based on sequence homology.
  3. What is the concentration of NBP1-33780?
    • The concentration of the lot number DB003-02-5B is 4.9 mg/ml

Secondary Antibodies

 

Isotype Controls

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Research Areas for iNOS Antibody (NBP1-33780)

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Sandra Lourenssen
04/18/2018
Application: IHC-P
Species: Porcine

Bioinformatics

Gene Symbol NOS2
Entrez
Uniprot