HIF-1 alpha Antibody Pair

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal

Order Details

HIF-1 alpha Antibody Pair Summary

Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.

  • Antibody pair for IP: rabbit polyclonal anti-HIF1A (300 ul)
  • Antibody pair for WB: mouse purified polyclonal anti-HIF1A (50 ug)
  • Clonality
    Polyclonal
    Gene
    HIF1A

    Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Preservative
    No Preservative

    Notes

    Quality control test: Immunoprecipitation-Western Blot (IP-WB).

    This product is produced by and distributed for Abnova, a company based in Taiwan.

    Alternate Names for HIF-1 alpha Antibody Pair

    • ARNT-interacting protein
    • Basic-helix-loop-helix-PAS protein MOP1
    • BHLHE78
    • Class E basic helix-loop-helix protein 78
    • HIF 1A
    • HIF1 alpha
    • HIF-1 alpha
    • HIF1A
    • HIF-1a
    • HIF-1-alpha
    • HIF1-alpha
    • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor)
    • hypoxia-inducible factor 1-alpha
    • Member of PAS protein 1
    • member of PAS superfamily 1
    • MOP1HIF1-ALPHA
    • PAS domain-containing protein 8
    • PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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    Publications for HIF-1 alpha Antibody Pair (H00003091-PW1) (0)

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol

    FAQs for HIF-1 alpha Antibody Pair (H00003091-PW1). (Showing 1 - 4 of 4 FAQ).

    1. Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
      • HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
    2. Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
      • All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, as desired by you, I am suggesting you four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products has been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449 ) (cited in at least 31 peer reviewed publications).
    3. I would like to know, does it exist path for detection HIF 1 in the venous blood before and after revascularization of the leg?
      • We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
    4. What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
      • The theoretical molecular weight of HIF 1-alpha is ~93kDa, however, you will likely see a band between 100-120kDa due to phosphorylation.

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    Blogs on HIF-1 alpha. Showing 1-10 of 26 blog posts - Show all blog posts.

    The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
    Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

    The relationship between Ki67 and HIF-1 in cancer
    Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

    Controls for HIF-1 Alpha's WB, ICC-IF, IHC, IP & FLOW Analysis
    Tips on positive and negative controls for HIF-1 alpha antibodies is one of the most Frequently Asked Questions on Hypoxia and HIFs. Here are top 5 suggestions from Novus Biologicals: The degradation of HIF1 alpha is the most common issue whic...  Read full blog post.

    Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
    Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

    HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
    HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

    HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
    By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

    HIF-1 alpha - sensing and responding to changing oxygen levels
    Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

    Comprehensive Autophagy Research Tools - New Catalog Available Now!
    Autophagy, a protein degradation process through autophagosome-lysosomal pathway, is important for cellular homeostasis and plays a role in many diseases. To help researchers learn more about this process and the products available for its study, N...  Read full blog post.

    HIF-1 beta: A dimerization partner of HIF-1 alpha required for an adaptive response to hypoxia
    Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia-indu...  Read full blog post.

    HIF-1 Alpha: Infographic
    Encoded by the HIF1A gene, HIF-1 alpha has a critical role in cellular response to hypoxia. In hypoxic conditions, HIF-1 alpha activates the transcription of several genes to facilitate metabolic reaction for lack of oxygen. In normoxic conditions, HI...  Read full blog post.

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    Bioinformatics

    Gene Symbol HIF1A
    Entrez