HIF-1 alpha Antibody Pack

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Western Blot: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of mouse HIF1-alpha on hypoxia treated MEFs using NB 100-449.
Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos ...read more
Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-901IHC] - Staining of placenta, villi using.
Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-901IHC] - Analysis of HIF1-alpha in CoCl2 Treated vs. Untreated HeLa Cells. Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% ...read more
Western Blot: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts.
Western Blot: HIF-1 alpha Antibody Pack [NB100-901IHC] - On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold ...read more
Western Blot: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic) NB100-449.
Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. ...read more
Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. ...read more
Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line. Photos courtesy of Susan Alexander and Hattie ...read more
Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-901IHC] - Staining of HIF-1 alpha in human kidney.
Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-901IHC] - Staining in hypoxia-induced human placenta.
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody Pack [NB100-901IHC] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.
Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-901IHC] - Staining of mouse brain, neurons using NB100-449.
Immunoprecipitation: HIF-1 alpha Antibody Pack [NB100-901IHC] - Immunoprecipitation of HIF-1alpha using NB100-123. Heavy and light chains are also detected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Order Details

HIF-1 alpha Antibody Pack Summary

Immunogen
NB 100-131: Human HIF-1 alpha amino acids 329-530. NB100-123: Fusion protein containing amino acids 432-528 of human HIF-1alpha. NB100-449: peptide between residues 775 and the C-terminus (residue 826) of human HIF-1 alpha NB100-479: A fusion protein
Specificity
These antibodies are specific for HIF-1alpha.
Gene
HIF1A

Applications/Dilutions

Application Notes
Immunohistochemical staining of paraffin-embedded and frozen sections (only NB 100-131 has been used on frozen sections). Antigen retrieval is strongly recommended. With the exception of NB100-449 and NB100-479 (which are also recommended for Western Blot), these antibodies work for Western blot analysis but are not specifically recommended for this application. A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background.
Publications
Read Publications using NB100-901IHC.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Notes

1 sample size vial each of: NB100-479 (0.025mL), NB100-449 (0.025mL), NB100-123 (0.05mL) and NB100-131 (0.05mL).

Alternate Names for HIF-1 alpha Antibody Pack

  • ARNT-interacting protein
  • Basic-helix-loop-helix-PAS protein MOP1
  • BHLHE78
  • Class E basic helix-loop-helix protein 78
  • HIF 1A
  • HIF1 alpha
  • HIF-1 alpha
  • HIF1A
  • HIF-1a
  • HIF-1-alpha
  • HIF1-alpha
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor)
  • hypoxia-inducible factor 1-alpha
  • Member of PAS protein 1
  • member of PAS superfamily 1
  • MOP1HIF1-ALPHA
  • PAS domain-containing protein 8
  • PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)

Background

Our HIF-1 alpha sample pack provides a convenient way to determine the optimal HIF-1 alpha antibody for your specific species and tissues. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein that activates gene transcription in response to reduced cellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, heme oxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are induced by hypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulate hypoxia-inducible factors may be related to tumor-related angiogenesis in certain cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
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NB300-605
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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP

Publications for HIF-1 alpha Antibody Pack (NB100-901IHC)(5)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HIF-1 alpha Antibody Pack (NB100-901IHC). (Showing 1 - 5 of 5 FAQs).

  1. Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
    • HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
  2. Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
    • All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, as desired by you, I am suggesting you four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products has been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449 ) (cited in at least 31 peer reviewed publications).
  3. I would like to know, does it exist path for detection HIF 1 in the venous blood before and after revascularization of the leg?
    • We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
  4. What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
    • The theoretical molecular weight of HIF 1-alpha is ~93kDa, however, you will likely see a band between 100-120kDa due to phosphorylation.
  5. We have received an enquiry regarding HIF1 alpha antibody panel, product code NB 100-901IHC. I would appreciate if you are able to provide the WB testing protocol for this antibody.
    • There are four antibodies in this pack. We have specific western blot protocols for 3 of the 4 antibodies, as follows: Western Blot protocol specific for NB100-123, Western Blot protocol specific for NB100-479, Western Blot protocol specific for NB100-449. You may find our general western blot protocol useful.

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Blogs on HIF-1 alpha. Showing 1-10 of 26 blog posts - Show all blog posts.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Controls for HIF-1 Alpha's WB, ICC-IF, IHC, IP & FLOW Analysis
Tips on positive and negative controls for HIF-1 alpha antibodies is one of the most Frequently Asked Questions on Hypoxia and HIFs. Here are top 5 suggestions from Novus Biologicals: The degradation of HIF1 alpha is the most common issue whic...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

Comprehensive Autophagy Research Tools - New Catalog Available Now!
Autophagy, a protein degradation process through autophagosome-lysosomal pathway, is important for cellular homeostasis and plays a role in many diseases. To help researchers learn more about this process and the products available for its study, N...  Read full blog post.

HIF-1 beta: A dimerization partner of HIF-1 alpha required for an adaptive response to hypoxia
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia-indu...  Read full blog post.

HIF-1 Alpha: Infographic
Encoded by the HIF1A gene, HIF-1 alpha has a critical role in cellular response to hypoxia. In hypoxic conditions, HIF-1 alpha activates the transcription of several genes to facilitate metabolic reaction for lack of oxygen. In normoxic conditions, HI...  Read full blog post.

Showing 1-10 of 26 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol HIF1A
Entrez