Reactivity | Hu, Mu, RtSpecies Glossary |
Applications | WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, KD |
Immunogen | For NB100-131: Human HIF-1 alpha amino acids 329-530. For NB100-123: Fusion protein containing amino acids 432-528 of human HIF-1alpha. For NB100-449: peptide between residues 775 and the C-terminus (residue 826) of human HIF-1 alpha. For NB100-479: A fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [Uniprot# Q61221]. |
Specificity | These antibodies are specific for HIF-1 alpha. |
Gene | HIF1A |
Application Notes | Immunohistochemical staining of paraffin-embedded and frozen sections (only NB100-131 has been used on frozen sections). Antigen retrieval is strongly recommended. With the exception of NB100-449 and NB100-479 (which are also recommended for Western blot), these antibodies work for Western blot analysis but are not specifically recommended for this application. A starting dilution of 1:1000 is recommended with titration up or down depending upon the signal and background. See individual datasheets for validated applications. |
|
Publications |
|
Storage | Store at 4C. Do not freeze. |
Diseases for HIF-1 alpha Antibody Pack (NB100-901IHC)Discover more about diseases related to HIF-1 alpha Antibody Pack (NB100-901IHC).
| Pathways for HIF-1 alpha Antibody Pack (NB100-901IHC)View related products by pathway.
|
Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post. |
mTOR Signaling and the Tumor Microenvironment By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein... Read full blog post. |
Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric... Read full blog post. |
Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy... Read full blog post. |
Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve... Read full blog post. |
The role of HIF-1 Alpha signaling in the retina under hypoxic conditions Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ... Read full blog post. |
The relationship between Ki67 and HIF-1 in cancer Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life. First discovered to be located to dividing cells,... Read full blog post. |
Controls for HIF-1 Alpha's WB, ICC-IF, IHC, IP & FLOW Analysis Tips on positive and negative controls for HIF-1 alpha antibodies is one of the most Frequently Asked Questions on Hypoxia and HIFs. Here are top 5 suggestions from Novus Biologicals: The degradation of HIF1 alpha is the most common issue whic... Read full blog post. |
Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel... Read full blog post. |
Gene Symbol | HIF1A |