beta-Actin Antibody

Images

 
Western Blot: beta-Actin Antibody [NB600-505] - Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 ug per lane. Primary antibody: Beta Actin ...read more
Immunohistochemistry: beta-Actin Antibody [NB600-505] - staining y at 1:200 shows detection of actin at the neuromuscular juntion of rana pipiens tissue. Formalin fixed paraffin embedded sections.
Western Blot: beta-Actin Antibody [NB600-505] - Detection of actin Lane 1: HeLa cell lysate, Lane 2: rat brain, Lane 3: mouse brain, Lane 4: 3T3 cell lysate.
Western Blot: beta-Actin Antibody [NB600-505] - analysis of Beta Actin on HeLa and LNCaP cell lysates(25ug/lane) using anti-Beta Actin antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, GP, Gt, Ha, Pm, ShSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.3 mg/ml

Order Details

beta-Actin Antibody Summary

Immunogen
Synthetic peptide: KQEYDESGPSC conjugated to KLH, corresponding to amino acids 359-368 of Human beta Actin.
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:4000
  • ELISA 1:10000-1:40000
  • Immunocytochemistry/Immunofluorescence 1:500-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding - beta actin by western blotting in the appropriate cell lysate or extract.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
293 Lysate (NB800-PC6)
HeLa Lysate (NB800-PC1)
Lung Lysate (NB820-59432)
Brain Lysate (NB820-59657)
Reviewed Applications
Read 1 Review rated 5
using
NB600-505 in the following applications:

Publications
Read Publications using
NB600-505 in the following applications:

  • EM
    1 publication
  • 2 publications
  • WB
    3 publications

Reactivity Notes

Expected to cross-react with a wide range of species including most vertebrates and some yeast due to sequence identity.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta-Actin Antibody

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Actin Antibody (NB600-505)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: EM, ICC/IF, WB.


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EM
(1)
ICC/IF
(2)
WB
(3)
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Human
(1)
Mouse
(2)
Rat
(2)
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Showing Publications 1 - 10 of 10.
Publications using NB600-505 Applications Species
Bigot P, Colli LM, Machiela MJ et al. Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41 Nat Commun 2016 Jul 07 [PMID: 27384883] (WB) WB
Gu Y, Helenius M, Vaananen K et al. BRCA1-deficient breast cancer cell lines are resistant to MEK inhibitors and show distinct sensitivities to 6-thioguanine. Sci Rep Jun 17 2016 [PMID: 27313062] (WB) WB
Gu Y, Bouwman P, Greco D et al. Suppression of BRCA1 sensitizes cells to proteasome inhibitors. Cell Death Dis 2014 Dec 18 [PMID: 25522274] (WB) WB
Chang M, Patel V, Gwede M et al. IL-1B induces p62/SQSTM1 and represses androgen receptor expression in prostate cancer cells J. Cell. Biochem. 2014 Aug 07 [PMID: 25103771]
Gerrits Han, Parade Marc C B C, Koonen-Reemst Annemie M C B et al. Reversible infertility in a liver receptor homologue-1 (LRH-1)-knockdown mouse model. Reprod Fertil Dev. 2014 Jan 01 [PMID: 23425349] (Mouse) Mouse
Mitchell L, Lau A, Lambert J-P et al. Regulation of septin dynamics by the Saccharomyces cerevisiae lysine acetyltransferase NuA4. PLoS ONE;6(10). 2011 [PMID: 21984913]
Denis N, Palmer-Smith H, Elisma F et al. Quantitative proteomic analysis of PCSK9 gain of function in human hepatic HuH7 cells. J Proteome Res 2011 Apr 1 [PMID: 21332221]
Ozkucur N, Perike S, Sharma P et al. Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness. BMC Cell Biol. 2011 Jan [PMID: 21255452] (ICC/IF, Rat, Human) ICC/IF Rat, Human
Young JNS, Guttman JA, Vaid KS et al. Tubulobulbar complexes are intercellular podosome-like structures that internalize intact intercellular junctions during epithelial remodeling events in the rat testis. Biol Reprod;80(1):162-74. 2009 Jan. [PMID: 18799754] (ICC/IF, EM, Rat, Mouse) ICC/IF, EM Rat, Mouse
Lee, Y et al. VEGF Receptor Phosphorylation Status Apoptosis is Modulated by a Green Tea Component, Epigallocatechin-3-Gallate (EGCG), in B-Cell Chronic Lymphocytic Leukemia. Blood 104: 788-794. 2004 [PMID: 14996703]

Review for beta-Actin Antibody (NB600-505) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-505:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot beta-Actin NB600-505
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reviewed by:
Anonymous
WB Human 10/07/2014
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Summary

ApplicationWestern Blot
Sample TestedHela and LNCap cell lysates
SpeciesHuman

Blocking

Blocking DetailsBSA (5%) in PBS buffer

Primary Anitbody

Dilution Ratio1:1000 diluted in PBS buffer

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP conjugated secondary antibody (Biopioneer)
Secondary Manufacturer Cat#GAR21002-1
Secondary Concentration1:15000

Details

Detection NotesDeveloped using FemtoLucent Plus HRP Chemiluminescent Kit with a 10 sec exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NB600-505). (Showing 1 - 5 of 5 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
  5. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence corresponding to amino acids 359 - 368 of beta Actin.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for beta-Actin Antibody (NB600-505)

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Blogs on beta-Actin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Recent Reviews

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1
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Anonymous
10/07/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTB
Entrez
Uniprot