293 Whole Cell Lysate


Western Blot: 293 Whole Cell Lysate [NB800-PC6] - Lane 1: Coomassie stain of 0.02 ml 293 extract (NB 800-PC6), separated in a 4-20% gradient gel under reducing conditions. Lane 2: Molecular weight standard.
SDS-Page: 293 Whole Cell Lysate [NB800-PC6] - Coommassie stained SDS-PAGE of 20 ug of (1) Hela WCL, (2) Jurkat WCL, (3) HEK 293 WCL, (4) MCF-7 WCL, (5) A 549 WCL, (6) Raji WCL separated using a 4-20% gradient gel under ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, PAGE
2.0 mg/ml

Order Details

293 Whole Cell Lysate Summary

293 whole cell extract lysate


  • SDS-Page
  • Western Blot
Application Notes
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents . Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 2x Laemmli Sample Buffer (125 mM Tris HCl, 4% SDS, 50% Glycerol and 0.02% bromophenol blue, pH 6.8)
2.0 mg/ml

Lysate Details for Array

Subcellular Fraction


This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for 293 Lysate (NB800-PC6) (0)

There are no publications for 293 Lysate (NB800-PC6).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 293 Lysate (NB800-PC6) (0)

There are no reviews for 293 Lysate (NB800-PC6). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for 293 Lysate (NB800-PC6). (Showing 1 - 1 of 1 FAQ).

  1. In NB800-PC6, were the 293 cells transfected with WT1 expression plasmids? We have 293 cell lysates and it showed negative WT1 expression. Just want to make sure if our antibody is not working.
    • This protein lysate was derived from human HEK293 cells (without any transfection) and it is a whole cell lysate. WT1's mRNA is expressed significantly in HEK294 cells protein atlas site and given the fact that our Wilms Tumor 1 antibody (6F-H2) [NB110-60011] showed positive bands on HEK293 cells lysate, you should be good for considering this lysate as a positive control for WT1.

Additional 293 Products

Array NB800-PC6

Blogs on 293

There are no specific blogs for 293, but you can read our latest blog posts.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our 293 Whole Cell Lysate and receive a gift card or discount.