293 Whole Cell Lysate (NB800-PC6)

Images

 
Western Blot: 293 Whole Cell Lysate [NB800-PC6] - Lane 1: Coommassie stain of 0.02 ml 293 extract (NB 800-PC6), separated in a 4-20% gradient gel under reducing conditions. Lane 2: Molecular weight standard.
SDS-Page: 293 Whole Cell Lysate [NB800-PC6] - Coommassie stained SDS-PAGE of 20 ug of (1) Hela WCL, (2) Jurkat WCL, (3) HEK 293 WCL, (4) MCF-7 WCL, (5) A 549 WCL, (6) Raji WCL separated using a 4-20% gradient gel under ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PAGE

Order Details

293 Whole Cell Lysate Summary

Specificity
293 whole cell extract lysate

Applications/Dilutions

Application Notes
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents. Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)

Lysate Details for 293

Type
Cell
Subcellular Fraction
Whole

Background

This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for 293 Lysate (NB800-PC6). (Showing 1 - 1 of 1 FAQ).

  1. In NB800-PC6, were the 293 cells transfected with WT1 expression plasmids? We have 293 cell lysates and it showed negative WT1 expression. Just want to make sure if our antibody is not working.
    • This protein lysate was derived from human HEK293 cells (without any transfection) and it is a whole cell lysate. WT1's mRNA is expressed significantly in HEK294 cells protein atlas site and given the fact that our Wilms Tumor 1 antibody (6F-H2) [NB110-60011] showed positive bands on HEK293 cells lysate, you should be good for considering this lysate as a positive control for WT1.

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293 NB800-PC6

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Calculators

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