Mouse Brain Whole Tissue Lysate (Adult Whole Normal) Summary
Total Protein - Mouse Normal Tissue: Brain
- Western Blot 10 ug- 20 ug
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Lysate Details for Array
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Publications for Brain Lysate (NB820-59657) (0)
There are no publications for Brain Lysate (NB820-59657).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Brain Lysate (NB820-59657) (0)
There are no reviews for Brain Lysate (NB820-59657).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Brain Lysate (NB820-59657). (Showing 1 - 2 of 2 FAQ).
We are seeking a normal brain tissue lysate for control of Parkinson’s disease. In your website, there is Human Brain Parietal Lobe Whole Tissue Lysate (Adult Parkinson's) 1.0mg (NB820-59414). If you have any normal brain tissue lysate, please let me know.
- Please see this link to all of our normal human brain tissue lysates.
Can this product be used as a positive control for anti-GluR2 (C-Term) and anti-GluR3 (N-term) antibodies?
- We haven't tested these lysates for the specific expression of any single target, but I would expect GLUR2 and GLUR3 to be present. The only concern I have is how highly expressed these proteins would be in these samples and if they're abundant enough to serve as a good positive control. The donors for NB820-59657 vary, and you may see some change in signal if you were to probe multiple lots. I think that the best positive control (depending on your experiment) may be an over-expression lysate or recombinant protein.