Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Images

 
Western Blot: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Western blot analysis of GAPDH (NB300-323) using whole normal mouse brain lysate [NB820-59657].
SDS-Page: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Ponceau S staining of whole normal mouse brain lysate [NB820-59657].
SDS-Page: Mouse Brain Whole Tissue Lysate (Whole Normal) [NB820-59657] - Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP, PAGE
Concentration
5 mg/ml

Order Details

Mouse Brain Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Mouse Normal Tissue: Brain

Applications/Dilutions

Dilutions
  • Western Blot 10 ug- 20 ug
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Brain
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Brain Lysate (NB820-59657). (Showing 1 - 2 of 2 FAQ).

  1. We are seeking a normal brain tissue lysate for control of Parkinson’s disease. In your website, there is Human Brain Parietal Lobe Whole Tissue Lysate (Adult Parkinson's) 1.0mg (NB820-59414). If you have any normal brain tissue lysate, please let me know.
    • Please see this link to all of our normal human brain tissue lysates.
  2. Can this product be used as a positive control for anti-GluR2 (C-Term) and anti-GluR3 (N-term) antibodies?
    • We haven't tested these lysates for the specific expression of any single target, but I would expect GLUR2 and GLUR3 to be present. The only concern I have is how highly expressed these proteins would be in these samples and if they're abundant enough to serve as a good positive control. The donors for NB820-59657 vary, and you may see some change in signal if you were to probe multiple lots. I think that the best positive control (depending on your experiment) may be an over-expression lysate or recombinant protein.

Other Available Formats

NB820-59657

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