VEGF Antibody (VG76e)

Images

 
Western Blot: VEGF Antibody (VG76e) [NB100-648] - Analysis of VEGF in human kidney protein (30ug).
Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) [NB100-648] - IHC analysis of VEGF in fetal sheep brain tissue. Image courtesy of anonymous customer review.

Product Details

Summary
Reactivity Hu, Po, Bv, ShSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, RIA
Clone
VG76e
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

VEGF Antibody (VG76e) Summary

Immunogen
Recombinant human VEGF189 expressed in E.coli [UniProt# P15692]
Localization
This antibody is highly specific, but only weakly stains transfected cells (5-10%).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VEGFA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Radioimmunoassay
Application Notes
This VEGF antibody is useful for Western blot, Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry (Formalin-fixed paraffin-embedded sections).Radioimmunoassay was reported in scientific literature. In Western blot a band is observed between 38-44 kDa. With IHC-paraffin sections microwave treatment with Tris HCl pH 10 buffer (NOT citrate buffer) is necessary. VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
VEGF Lysate (NBL1-17712)
Reviewed Applications
Read 5 Reviews rated 3.8
using
NB100-648 in the following applications:

Publications
Read Publications using
NB100-648 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • RIA
    1 publication
  • WB
    4 publications

Reactivity Notes

Human. Mixed results in Cow and Pig (IHC only). Customers have reported success staining sheep tissue (see review).

Alternate Names for VEGF Antibody (VG76e)

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein growth factor that plays a critical role in angiogenesis, vasculogenesis and endothelial cell growth through induction of endothelial cell proliferation and blood vessels permeabilization, cell migration promotion as well as inhibition of apoptosis. VEGF can bind to FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Its isoforms VEGF189, VEGF165 and VEGF121 are widely expressed, whereas, other isoforms VEGF206 and VEGF145 are not very common. The basic isoform VEGF189 is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. VEGF bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C/-D (may gain VEGFR-2 binding ability via proteolytic processing) and is essential for lymphangiogenic signaling. VEGF is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and VEGF polymorphisms are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, TCS, LA
AF3628
Species: Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready
MAB8967
Species: Hu
Applications: WB, Simple Western, ICC
MAB2055
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow
NB110-41083
Species: Hu
Applications: WB, ChIP, ELISA
NB600-1071
Species: Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-47842
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
NB100-648
Species: Hu, Po, Bv, Sh
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, RIA

Publications for VEGF Antibody (NB100-648)(11)

We have publications tested in 4 confirmed species: Human, Rat, Human , Porcine.

We have publications tested in 4 applications: IHC, IHC-P, RIA, WB.


Filter By Application
IHC
(2)
IHC-P
(1)
RIA
(1)
WB
(4)
All Applications
Filter By Species
Human
(3)
Rat
(1)
Human
(1)
Porcine
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-648 Applications Species
Iwai R, Nemoto Y, Nakayama Y. Preparation and characterization of directed, one-day-self-assembled millimeter-size spheroids of adipose-derived mesenchymal stem cells. J Biomed Mater Res A 2015 Sep 19 [PMID: 26386244] (IHC, Rat) IHC Rat
Nishimiya K, Matsumoto Y, Shindo T et al. Association of Adventitial Vasa Vasorum and Inflammation With Coronary Hyperconstriction After Drug-Eluting Stent Implantation in Pigs In Vivo Circ. J. 2015 Jun 01 [PMID: 26027445] (IHC-P, Porcine) IHC-P Porcine
Xu X, Mao W, Chai Y et al. Angiogenesis Inhibitor, Endostar, Prevents Vasa Vasorum Neovascularization in a Swine Atherosclerosis Model. J Atheroscler Thromb 2015 May 26 [PMID: 26016418] (WB) WB
Yoshizawa S, Brown A, Barchowsky A, Sfeir C. Role of magnesium ions on osteogenic response in bone marrow stromal cells. Connect Tissue Res. 2014 Aug 01 [PMID: 25158202] (WB, Human) WB Human
Yoshizawa S, Brown A, Barchowsky A. Magnesium ion stimulation of bone marrow stromal cells enhances osteogenic activity, simulating the effect of magnesium alloy degradation. Acta Biomater 2014 Feb 24 [PMID: 24512978] (WB, Human) WB Human
Yu R, Dhall D, Nissen NN et al. Pancreatic neuroendocrine tumors in glucagon receptor-deficient mice. PLoS ONE 20116(8). [PMID: 21853126]
Mannheim D et al. Impaired myocardial perfusion reserve in experimental hypercholesterolemia is independent of myocardial neovascularization. Am J Physiol Heart Circ Physiol;2925:H2449-58. 2007 May. [PMID: 17208989] (WB, Porcine) WB Porcine
Brown NS, Streeter EH, Jones A, Harris AL, Bicknell R. Cooperative stimulation of vascular endothelial growth factor expression by hypoxia and reactive oxygen species: the effect of targeting vascular endothelial growth factor and oxidative stress in an orthotopic xenograft model of bladder carcinoma. Br J Cancer;929:1696-701. 2005 May 9. [PMID: 15841086]
Collingridge DR, Carroll VA, Glaser M, Aboagye EO, Osman S, Hutchinson OC, Barthel H, Luthra SK, Brady F, Bicknell R, Price P, Harris AL. The development of [124I]iodinated-VG76e: a novel tracer for imaging vascular endothelial growth factor in vivo using positron emission tomography. Cancer Res. 6220:5912-9. 2002 Oct 15. [PMID: 12384557] (RIA, Human) RIA Human
Fraser HM, Dickson SE, Lunn SF, Wulff C, Morris KD, Carroll VA, Bicknell R. Suppression of luteal angiogenesis in the primate after neutralization of vascular endothelial growth factor. Endocrinology;1413:995-1000. 2000 Mar. [PMID: 10698175]
Show All 11 Publications.

Reviews for VEGF Antibody (NB100-648) (5) 3.85

Average Rating: 3.8
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Other.
We have 5 reviews tested in 4 application: WB, IHC-P, IF, ICC.

Reviews using NB100-648:
Filter by Applications
WB
(1)
IHC-P
(2)
IF
(1)
ICC
(1)
All Applications
Filter by Species
Other
(5)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Hima S
WB Other 09/14/2014
View

Summary

ApplicationWestern Blot
Sample TestedBovine Lysate
SpeciesOther
Immunohistochemistry-Paraffin VEGF NB100-648
Enlarge
reviewed by:
Anonymous
IHC-P Other 07/29/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedsheep tonsil
SpeciesOther
CommentsDid not work with low pH antigen retrieval, required overnight incubation of primary antibody.

Primary Anitbody

Dilution Ratio1:200

Details

Fixation DetailsFFPE pH 9 antigen retrieval overnight at 60C

Comments

CommentsDid not work with low pH antigen retrieval, required overnight incubation of primary antibody.
Immunofluorescence VEGF NB100-648
Enlarge
reviewed by:
Ewa Kaniewska
IF Other 05/13/2014
View

Summary

ApplicationImmunofluorescence
SpeciesOther
FileView PDF
 
reviewed by:
Ewa Kaniewska
ICC Other 05/13/2014
View

Summary

ApplicationImmunocytochemistry
Sample Testedpig endothelial cells
SpeciesOther
Lot747/07
Immunohistochemistry-Paraffin VEGF NB100-648
Enlarge
reviewed by:
Ana Baburamani
IHC-P Other 05/04/2011
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFetal Sheep Brain Tissue
SpeciesOther
Lot467/04
CommentsThis product has been successfully used in paraffin embedded tissue for fetal sheep brain (for the first time). The staining is faint, but consistent. A strong antigen retrieval (TBS pH 10) is required, but little background appears. Detection Method: DAB, Positive Control: Spiny Mouse Placenta, Negative Control: No Primary, Deparaffinization: Xylene, 100% Etoh, 70% Etoh, Antigen Retrieval: TBS (ph 10), 3 x 10 min in microwave at 100 degrees Celsius

Blocking

Blocking DetailsBlocking Buffer: Normal Goat Serum Block Buffer, Blocking Time: 45 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:200, Incubation Dilution Buffer: True Vision (Sapphire Bioscience), Incubation Time/Temp: overnight at 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Rabbit anti-Mouse, Secondary Ab Dilution: 1:200
Secondary Manufacturer Cat#Vector

Details

Detection NotesPlease see Comments
Fixation DetailsSample Preparation: PFA fixed, parafin embedded, Deparaffinization: Xylene, 100% Etoh, 70% Etoh

Comments

CommentsThis product has been successfully used in paraffin embedded tissue for fetal sheep brain (for the first time). The staining is faint, but consistent. A strong antigen retrieval (TBS pH 10) is required, but little background appears. Detection Method: DAB, Positive Control: Spiny Mouse Placenta, Negative Control: No Primary, Deparaffinization: Xylene, 100% Etoh, 70% Etoh, Antigen Retrieval: TBS (ph 10), 3 x 10 min in microwave at 100 degrees Celsius

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VEGF Antibody (NB100-648). (Showing 1 - 3 of 3 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
  2. I have a question about your VEGF (AF214570) and its antibody (NB100-648). How long are their expiry date?
    • We guarantee our antibodies for 6 months from the date of purchase, but they should function much longer than that.
  3. Can you tell me if NB100-648 was raised against a peptide sequence of the mature VEGF protein, or against the whole protein? Do you know what aspect of the protein structure of VEGF that the antibody recognizes? Can you tell me if the antibody will detect VEGF-A isoforms such as 110,121,145,148, etc?
    • The lab have informed me that the immunogen used was full length VEGF (subtype 189) protein. Unfortunately we have not tested if this detects all isoforms of VEGF-A.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-648AF405
Alexa Fluor 488 Labeled NB100-648AF488
Alexa Fluor 647 Labeled NB100-648AF647
Alexa Fluor 700 Labeled NB100-648AF700
Biotin Labeled NB100-648B
DyLight 350 Labeled NB100-648UV
DyLight 405 Labeled NB100-648V
DyLight 488 Labeled NB100-648G
DyLight 550 Labeled NB100-648R
DyLight 650 Labeled NB100-648C
DyLight 680 Labeled NB100-648FR
DyLight 755 Labeled NB100-648IR
FITC Labeled NB100-648F
HRP Labeled NB100-648H
PerCP Labeled NB100-648PCP

Additional VEGF Antibody (VG76e) Products

Related Products by Gene

Bioinformatics Tool for VEGF Antibody (NB100-648)

Discover related pathways, diseases and genes to VEGF Antibody (NB100-648). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VEGF Antibody (NB100-648)

Discover more about diseases related to VEGF Antibody (NB100-648).
 

Pathways for VEGF Antibody (NB100-648)

View related products by pathway.

PTMs for VEGF Antibody (NB100-648)

Learn more about PTMs related to VEGF Antibody (NB100-648).
 

Research Areas for VEGF Antibody (NB100-648)

Find related products by research area.

Blogs on VEGF. Showing 1-10 of 16 blog posts - Show all blog posts.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

Contact Information

Product PDFs

Recent Reviews

3.8
5
1
4
2
3
2
2
0
1
0

 
Hima S
09/14/2014
Application: WB
Species: Other

Anonymous
07/29/2014
Application: IHC-P
Species: Other

Ewa Kaniewska
05/13/2014
Application: IF
Species: Other

Bioinformatics

Gene Symbol VEGFA
Entrez
Uniprot

Customers Who Bought This Also Bought