Recombinant Human Angiopoietin-1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Angiopoietin-1 Protein Summary

Details of Functionality
Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. Kwak, H.J. et al. (1999) FEBS Letters 448:249. The ED50 for this effect is 10-40 ng/mL in the presence of 5 µg/mL of a
cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Mouse myeloma cell line, NS0-derived human Angiopoietin-1 protein
Ser20-Phe498, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser20
Structure / Form
Oligomer
Protein/Peptide Type
Recombinant Proteins
Gene
ANGPT1
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70 kDa, reducing conditions
Publications
Read Publications using
923-AN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-1 Protein

  • AGP1
  • AGPT
  • Ang1
  • ANG-1
  • angiopoietin 1
  • Angiopoietin-1
  • ANGPT1
  • KIAA0003

Background

Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system (1, 2). It contains a coiled-coil region and a C-terminal fibrinogen-like domain separated by a short flexible region (3, 4). Mature human Angiopoietin-1 shares 97% amino acid sequence identity with mouse and rat Angiopoietin-1. It is expressed by vascular smooth muscle cells and pericytes as an approximately 70 kDa molecule that associates into
disulfide-linked homotrimers, tetramers, and pentamers (3, 5). Angiopoietin-1 binds and activates the receptor tyrosine kinase Tie-2, and its association into tetramers is important for full Tie-2 activation (3, 4). Angiopoietin-1 ligation of Tie-2 on vascular endothelial cells (EC) induces the development and branching of blood vessels (6, 7). In sub-confluent EC (i.e. during angiogenesis), Angiopoietin-1 promotes EC motility and Tie-2 localization at the trailing edge of the cell (8). In confluent EC (i.e. in homeostasis), multimeric Angiopoietin-1 enhances vascular integrity by promoting the in trans homotypic association of Tie-2 between EC or with the substratum (8, 9). In addition, Angiopoietin-1 suppresses several VEGF-induced effects on the vasculature including endothelial permeability, stretch-induced release of Angiopoietin-2, and up-regulation of the leukocyte adhesion molecules VCAM-1, ICAM-1, and E-Selectin (10-12). Angiopoietin-1 also interacts with a variety of integrins and the extracellular matrix independently of Tie-2 (13, 14). These interactions support the adhesion, migration and stress resistance of EC, fibroblasts, and myocytes (13, 14). Angiopoietin-1 can protect against pulmonary arterial hypertension (5), reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium (15), and enhance tumor progression and metastasis (16).
  1. Koh, G.Y. (2012) Trends Mol. Med. 19:31.
  2. Suri, C. et al. (1996) Cell 87:1171.
  3. Davis, S. et al. (1996) Cell 87:1161.
  4. Kim, K.-T. et al. (2005) J. Biol. Chem. 280:20126.
  5. Kugathasan, L. et al. (2009) J. Exp. Med. 206:2221.
  6. Suri, C. et al. (1998) Science 282:468.
  7. Jeansson, M. et al. (2011) J. Clin. Invest. 121:2278.
  8. Saharinen, P. et al. (2008) Nat. Cell. Biol. 10:527.
  9. Fukuhara, S. et al. (2008) Nat. Cell Biol. 10:513.
  10. Jho, D. et al. (2005) Circ. Res. 96:1282.
  11. Korff, T. et al. (2012) Cardiovasc. Res. 94:510.
  12. Kim, I. et al. (2001) Circ. Res. 89:477.
  13. Carlson, T.R. et al. (2001) J. Biol. Chem. 276:26516.
  14. Dallabrida, S.M. et al. (2005) Cardiovasc. Res. 96:e8.
  15. Samuel, S.M. et al. (2010) Diabetes 59:51.
  16. Holopainen, T. et al. (2009) Cancer Res. 69:4656.

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Applications: Bioactivity

Publications for Angiopoietin-1 (923-AN)(50)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, N/A.

We have publications tested in 5 applications: Bioassay, Ctrl, ELISA Standard, In Vivo, WB Ctrl.


Filter By Application
Bioassay
(38)
Ctrl
(1)
ELISA Standard
(3)
In Vivo
(6)
WB Ctrl
(1)
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Human
(31)
Mouse
(10)
Rat
(2)
Bovine
(2)
N/A
(4)
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Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using 923-AN Applications Species
SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh Tie2 protects the vasculature against thrombus formation in systemic inflammation J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29360642] (Bioassay, Human) Bioassay Human
T Souma, BR Thomson, S Heinen, IA Carota, S Yamaguchi, T Onay, P Liu, AK Ghosh, C Li, V Eremina, YK Hong, AN Economides, D Vestweber, KG Peters, J Jin, SE Quaggin Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29358379] (Bioassay, Human) Bioassay Human
S La Porta, L Roth, M Singhal, C Mogler, C Spegg, B Schieb, X Qu, RH Adams, HS Baldwin, S Savant, HG Augustin Endothelial Tie1-mediated angiogenesis and vascular abnormalization promote tumor progression and metastasis J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29355844] (Bioassay, Human) Bioassay Human
C Daly, X Qian, C Castanaro, E Pasnikowsk, X Jiang, BR Thomson, SE Quaggin, N Papadopoul, Y Wei, JS Rudge, G Thurston, GD Yancopoulo, S Davis Angiopoietins bind thrombomodulin and inhibit its function as a thrombin cofactor Sci Rep, 2018;8(1):505. 2018 [PMID: 29323190] (Bioassay, Human) Bioassay Human
LY Pan, YF Chen, HC Li, LM Bi, WJ Sun, GF Sun, XF Zhang, K Xu, DX Feng Dachengqi Decoction Attenuates Intestinal Vascular Endothelial Injury in Severe Acute Pancreatitis in Vitro and in Vivo Cell. Physiol. Biochem., 2017;44(6):2395-2406. 2017 [PMID: 29262394] (Bioassay, Human) Bioassay Human
G Eich, M Bartosova, C Tischer, TT Wlodkowski, B Schaefer, S Pichl, N Kraewer, B Ranchin, K Vondrak, MC Liebau, T Hackert, CP Schmitt Bicarbonate buffered peritoneal dialysis fluid upregulates angiopoietin-1 and promotes vessel maturation PLoS ONE, 2017;12(12):e0189903. 2017 [PMID: 29253861] (Bioassay, Human) Bioassay Human
J Chen, Y Luo, H Hui, T Cai, H Huang, F Yang, J Feng, J Zhang, X Yan CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28827364] (Bioassay, Mouse) Bioassay Mouse
A Burnett, I Gomez, DD De Leon, M Ariaans, P Progias, RA Kammerer, G Velasco, M Marron, P Hellewell, V Ridger Angiopoietin-1 enhances neutrophil chemotaxis in vitro and migration in vivo through interaction with CD18 and release of CCL4 Sci Rep, 2017;7(1):2332. 2017 [PMID: 28539655] (Bioassay, Mouse) Bioassay Mouse
T Shlamkovic, L Aharon, WA Barton, N Papo Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy Oncotarget, 2017;8(20):33571-33585. 2017 [PMID: 28422724] (Ctrl, Human) Ctrl Human
JO Moore, MA Lemmon, KM Ferguson Dimerization of Tie2 mediated by its membrane-proximal FNIII domains Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28396397] (Bioassay, Human) Bioassay Human
Show All 50 Publications.

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Bioinformatics

Gene Symbol ANGPT1
Entrez
Uniprot