Hemoglobin Antibody - BSA Free

Whole cell lysate (50 µg) from K-562, and HeLa cells prepared using NETN lysis buffer. Antibody: Hemoglobin Antibody - BSA Free (NB110-41083) used for WB at 1:1000. Secondary: HRP-conjugated Donkey anti-Goat IgG Heavy and Light Chain Cross-Adsorbed Antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Hemoglobin Antibody - BSA Free Summary

• Description: The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
• Immunogen: Purified human hemoglobin A1 of adult red blood cell origin
• Specificity: This antibody reacts specifically with human hemoglobin A1, A2, F and S. There is no reaction with human serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HBA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:30000
  • Western Blot 1:1000 - 1:30000
• Application Notes: ELISA for coating plates 1:100 - 1:500.
• Control:

  Hemoglobin A1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Hemoglobin Antibody - BSA Free

• alpha one globin;alpha-1 globin;alpha-1-globin;alpha-2 globin chain;Alpha-globin;ALPHA-GLOBIN GENE;delta globin;Haemoglobin;Hb;HbA1c;hemoglobin alpha 1 globin chain;Hemoglobin alpha chain;Hemoglobin subunit alpha;hemoglobin, alpha 1
• Hemoglobin

Background

Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues.The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult Hemoglobin. The normal adult Hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta zero thalassemia. Reduced amounts of detectable beta globin causes beta plus thalassemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hemoglobin Antibody (NB110-41083)(11)

Publications using NB110-41083

• Chen HH, Wu CH, Tsai ML et al. Detection of Total and A1c-Glycosylated Hemoglobin in Human Whole Blood Using Sandwich Immunoassays on Polydimethylsiloxane-Based Antibody Microarrays Anal Chem 2012-09-26 [PMID: 22967096] (Chemotaxis, Human)
• Bethany F The role and mechanism of hemoglobin variation in Plasmodium falciparum sexual differentiation Thesis 2023-01-01 (Western Blot)
• Mehta S, Buyanbat A, Kai Y et al. Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells Cell chemical biology 2022-07-12 [PMID: 35839780]
• Kapralov et al. J.Biol.Chem 284 (44):30395-30407. 2009-01-01 [PMID: 19740759]
• Ma et al. PNAS 105 (35):13087-13092. 2008-01-01 [PMID: 18755895]
• Newton DA, Rao KM, Dluhy RA et al. Hemoglobin is expressed by alveolar epithelial cells. J Biol Chem 2006-03-01 [PMID: 16407281]
• Suber et al. Anal.Biochem 333 (2):351-357. 2004-01-01 [PMID: 15450812]
• Dagher et al. Diabetes 53 (9):2404-2411. 2004-01-01 [PMID: 15331552]
• Wang et al. PNAS 101 (31):11477-11482. 2004-01-01 [PMID: 15258287]
• Jerse et al. Infect.Immun. 70 2549-2558. 2002-01-01 [PMID: 11953395]
• Chen CJ, Mclean D, Thomas CE, Anderson JE, Sparling PF. Point mutations in HpuB enable gonococcal HpuA deletion mutants to grow on hemoglobin. J Bacteriol 184(2):420-6. 2002-01-01 [PMID: 11751818]

FAQs for Hemoglobin Antibody (NB110-41083). (Showing 1 - 1 of 1 FAQs).

1. Is this the same antibody as #NB110-41080, but the unconjugated format?
   • Yes, NB110-41083 is the same antibody as NB110-41080 but unconjugated.

Bioinformatics

• Gene Symbol: HBA1