Recombinant Human HGF Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human HGF Protein Summary

Details of Functionality
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 0.1‑0.5 ng/mL.
The specific activity of Recombinant Human HGF is approximately 1.5 x 103 IU/μg, which is calibrated against Recombinant Human HGF WHO International Standard (NIBSC code: 96/564).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human HGF protein
Gln32-Ser728
Accession #
N-terminal Sequence
alpha chain: No results obtained; Gln32 predicted
beta chain: Val495
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
HGF
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
53.7 kDa ( alpha chain), 26 kDa ( beta chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-70 kDa, non-reducing conditions
60 kDa, and 33-34 kDa, reducing conditions
Publications
Read Publications using
294-HG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HGF Protein

  • deafness, autosomal recessive 39
  • DFNB39
  • EC 3.4.21
  • EC 3.4.21.7
  • fibroblast-derived tumor cytotoxic factor
  • F-TCF
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • Hepatopoeitin-A
  • Hepatopoietin A
  • HGF
  • HGFB
  • HPTAhepatocyte growth factor
  • lung fibroblast-derived mitogen
  • Scatter factor
  • SF
  • SFhepatopoeitin-A

Background

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates human HGF isoforms that lack the proteinase‑like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH‑stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

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Publications for HGF (294-HG)(64)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Equine, Fish - Prionace glauca (blue shark), Hamster, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 5 applications: Bioassay, Ctrl, In Vivo, Scaffold Fabrication, activation.


Filter By Application
Bioassay
(55)
Ctrl
(1)
In Vivo
(2)
Scaffold Fabrication
(1)
activation
(1)
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Filter By Species
Human
(42)
Mouse
(6)
Rat
(5)
Equine
(1)
Fish - Prionace glauca (blue shark)
(1)
Hamster
(1)
Porcine
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 64. Show All 64 Publications.
Publications using 294-HG Applications Species
R Okamoto, K Takayama, N Akita, Y Nagamoto, D Hosokawa, S Iizuka, F Sakurai, H Suemizu, K Ohashi, H Mizuguchi Human iPS Cell-based Liver-like Tissue Engineering at Extrahepatic Sites in Mice as a New Cell Therapy for Hemophilia B Cell Transplant, 2018;27(2):299-309. 2018 [PMID: 29637813] (Bioassay, Human) Bioassay Human
WS Fagg, N Liu, MJ Yang, K Cheng, E Chung, JS Kim, G Wu, J Fair Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver Cell Transplant, 2017;26(12):1868-1877. 2017 [PMID: 29390880] (Bioassay, Mouse) Bioassay Mouse
K Yamane, T Mazaki, Y Shiozaki, A Yoshida, K Shinohara, M Nakamura, Y Yoshida, D Zhou, T Kitajima, M Tanaka, Y Ito, T Ozaki, A Matsukawa Collagen-Binding Hepatocyte Growth Factor (HGF) alone or with a Gelatin- furfurylamine Hydrogel Enhances Functional Recovery in Mice after Spinal Cord Injury Sci Rep, 2018;8(1):917. 2018 [PMID: 29343699] (In Vivo, Mouse) In Vivo Mouse
YJ Han, YH Kang, SB Shivakumar, D Bharti, YB Son, YH Choi, WU Park, JH Byun, GJ Rho, BW Park Stem Cells from Cryopreserved Human Dental Pulp Tissues Sequentially Differentiate into Definitive Endoderm and Hepatocyte-Like Cells in vitro Int J Med Sci, 2017;14(13):1418-1429. 2017 [PMID: 29200956] (Bioassay, Human) Bioassay Human
H Park, D Kim, E Kim, JK Sa, HW Lee, S Yu, J Oh, SH Kim, Y Yoon, DH Nam Tumor Inhibitory Effect of IRCR201, a Novel Cross-Reactive c-Met Antibody Targeting the PSI Domain Int J Mol Sci, 2017;18(9):. 2017 [PMID: 28902178] (Bioassay, Human) Bioassay Human
C Clémenson, C Chargari, W Liu, M Mondini, C Ferté, MF Burbridge, V Cattan, A Jacquet-Be, E Deutsch The MET/AXL/FGFR inhibitor S49076 impairs Aurora B activity and improves the antitumor efficacy of radiotherapy Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28619752] (Bioassay, Human) Bioassay Human
JG Camp, K Sekine, T Gerber, H Loeffler-W, H Binder, M Gac, S Kanton, J Kageyama, G Damm, D Seehofer, L Belicova, M Bickle, R Barsacchi, R Okuda, E Yoshizawa, M Kimura, H Ayabe, H Taniguchi, T Takebe, B Treutlein Multilineage communication regulates human liver bud development from pluripotency Nature, 2017;0(0):. 2017 [PMID: 28614297] (Bioassay, Human) Bioassay Human
C Zou, Y Lu, X Teng, S Wang, X Sun, F Huang, G Shu, X Huang, H Guo, Z Chen, J Zhang, YA Zhang MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys Sci Rep, 2017;7(1):2505. 2017 [PMID: 28566744] (Bioassay, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) Bioassay Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
F Sakurai, S Mitani, T Yamamoto, K Takayama, M Tachibana, K Watashi, T Wakita, S Iijima, Y Tanaka, H Mizuguchi Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection Sci Rep, 2017;7(0):45698. 2017 [PMID: 28374759] (Bioassay, Human) Bioassay Human
ZS Nevin, DC Factor, RT Karl, P Douvaras, J Laukka, MS Windrem, SA Goldman, V Fossati, GM Hobson, PJ Tesar Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes Am. J. Hum. Genet., 2017;100(4):617-634. 2017 [PMID: 28366443] (Bioassay, Human) Bioassay Human
Show All 64 Publications.

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Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

VEGFR-2 - A highly active kinase
VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry...  Read full blog post.

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Bioinformatics

Gene Symbol HGF
Entrez
Uniprot