Recombinant Human HGF Protein

Images

 
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 0.1‑0.5 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations

Recombinant Human HGF Protein Summary

Additional Information
Mammalian-expressed NS0 Version Available!
Details of Functionality
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 0.1‑0.5 ng/mL.
The specific activity of Recombinant Human HGF is approximately 1.5 x 103 IU/μg, which is calibrated against Recombinant Human HGF WHO International Standard (NIBSC code: 96/564).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human HGF protein
Gln32-Ser728
Accession #
N-terminal Sequence
alpha chain: No results obtained; Gln32 predicted
beta chain: Val495
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
HGF
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
53.7 kDa ( alpha chain), 26 kDa ( beta chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-70 kDa, non-reducing conditions
60 kDa, and 33-34 kDa, reducing conditions
Publications
Read Publications using
294-HG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HGF Protein

  • deafness, autosomal recessive 39
  • DFNB39
  • EC 3.4.21
  • EC 3.4.21.7
  • fibroblast-derived tumor cytotoxic factor
  • F-TCF
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • Hepatopoeitin-A
  • Hepatopoietin A
  • HGF
  • HGFB
  • HPTA
  • HPTAhepatocyte growth factor
  • lung fibroblast-derived mitogen
  • Scatter factor
  • SF
  • SFhepatopoeitin-A

Background

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates human HGF isoforms that lack the proteinase‑like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH‑stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Customers Who Viewed This Item Also Viewed...

AF527
Species: Mu
Applications: WB, IHC, Block, ICC
H00008731-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
NBP2-38464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF1280
Species: Mu
Applications: WB, Simple Western, IHC
NB600-41532
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
294-HG
Species: Hu
Applications: Bioactivity

Publications for HGF (294-HG)(83)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Equine, Fish - Prionace glauca (Blue Shark), Hamster, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Squirrel.

We have publications tested in 6 applications: Activation, Bioassay, Control, ELISA (Standard), In Vivo, Scaffold Fabrication.


Filter By Application
Activation
(1)
Bioassay
(73)
Control
(1)
ELISA (Standard)
(1)
In Vivo
(2)
Scaffold Fabrication
(1)
All Applications
Filter By Species
Human
(58)
Mouse
(6)
Rat
(6)
Equine
(1)
Fish - Prionace glauca (Blue Shark)
(1)
Hamster
(1)
Porcine
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Squirrel
(1)
All Species
Showing Publications 1 - 10 of 83. Show All 83 Publications.
Publications using 294-HG Applications Species
F Tonon, GG Giobbe, A Zambon, C Luni, O Gagliano, A Floreani, G Grassi, N Elvassore In vitro metabolic zonation through oxygen gradient on a chip Sci Rep, 2019;9(1):13557. 2019 [PMID: 31537830] (Bioassay, Human) Bioassay Human
YC Kim, J Lee, JN An, JH Kim, YW Choi, L Li, SH Kwon, MY Lee, B Lee, JG Jeong, SS Yu, CS Lim, YS Kim, S Kim, SH Yang, JP Lee Renoprotective effects of a novel cMet agonistic antibody on kidney fibrosis Sci Rep, 2019;9(1):13495. 2019 [PMID: 31530851] (Bioassay, Human) Bioassay Human
L Messerschm, S Fischer, P Wiedemann, A Bringmann, M Hollborn Osmotic induction of cyclooxygenase-2 in RPE cells: Stimulation of inflammasome activation Mol. Vis., 2019;25(0):329-344. 2019 [PMID: 31341381] (Bioassay, Human) Bioassay Human
G Yu, Z Wang, S Zeng, S Liu, C Zhu, R Xu, RE Liu Paeoniflorin Inhibits Hepatocyte Growth Factor- (HGF-) Induced Migration and Invasion and Actin Rearrangement via Suppression of c-Met-Mediated RhoA/ROCK Signaling in Glioblastoma Biomed Res Int, 2019;2019(0):9053295. 2019 [PMID: 30886866] (Bioassay, Human) Bioassay Human
R Graves-Dea, G Bogatcheva, S Rehman, Y Lu, JN Higginboth, B Singh Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer Oncotarget, 2019;10(13):1320-1333. 2019 [PMID: 30863492] (Bioassay, Human) Bioassay Human
GA Khan, S Bhagat, MI Alam PGE2 -induced migration of human brain endothelial cell is mediated though protein kinase A in cooperation of EP receptors J. Leukoc. Biol., 2019;0(0):. 2019 [PMID: 30835912] (Bioassay, Human) Bioassay Human
H Noguchi, C Miyagi-Shi, Y Nakashima, T Kinjo, N Kobayashi, I Saitoh, M Watanabe, AMJ Shapiro, T Kin Induction of Expandable Tissue-Specific Progenitor Cells from Human Pancreatic Tissue through Transient Expression of Defined Factors Mol Ther Methods Clin Dev, 2019;13(0):243-252. 2019 [PMID: 30828587] (Bioassay, Human) Bioassay Human
S Ashraf, S Bell, C O'Leary, P Canning, I Micu, JA Fernandez, M O'Hare, P Barabas, H McCauley, DP Brazil, AW Stitt, JG McGeown, TM Curtis CAMKII as a therapeutic target for growth factor-induced retinal and choroidal neovascularisation JCI Insight, 2019;0(0):. 2019 [PMID: 30721154] (Bioassay, Human) Bioassay Human
VS Hughes, DW Siemann Failures in preclinical and clinical trials of c-Met inhibitors: evaluation of pathway activity as a promising selection criterion Oncotarget, 2019;10(2):184-197. 2019 [PMID: 30719213] (Bioassay, Human) Bioassay Human
L Liu, L Leng, C Liu, C Lu, Y Yuan, L Wu, F Gong, S Zhang, X Wei, M Wang, L Zhao, L Hu, J Wang, H Yang, S Zhu, F Chen, G Lu, Z Shang, G Lin An integrated chromatin accessibility and transcriptome landscape of human pre-implantation embryos Nat Commun, 2019;10(1):364. 2019 [PMID: 30664750] (Bioassay, Human) Bioassay Human
Show All 83 Publications.

Reviews for HGF (294-HG) (0)

There are no reviews for HGF (294-HG). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for HGF (294-HG) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional HGF Products

Bioinformatics Tool for HGF (294-HG)

Discover related pathways, diseases and genes to HGF (294-HG). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HGF (294-HG)

Discover more about diseases related to HGF (294-HG).
 

Pathways for HGF (294-HG)

View related products by pathway.

PTMs for HGF (294-HG)

Learn more about PTMs related to HGF (294-HG).

Blogs on HGF.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

VEGFR-2 - A highly active kinase
VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human HGF Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol HGF
Uniprot