Recombinant Human HGF Protein


Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 0.1‑0.5 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human HGF Protein Summary

Additional Information
Mammalian-expressed NS0 Version Available!
Details of Functionality
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 0.1‑0.5 ng/mL.
The specific activity of Recombinant Human HGF is approximately 1.5 x 103 IU/μg, which is calibrated against Recombinant Human HGF WHO International Standard (NIBSC code: 96/564).
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human HGF protein
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession #
N-terminal Sequence
alpha chain: No results obtained; Gln32 predicted
beta chain: Val495
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
53.7 kDa ( alpha chain), 26 kDa ( beta chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
60-70 kDa, non-reducing conditions
60 kDa, and 33-34 kDa, reducing conditions
Read Publications using
294-HG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HGF Protein

  • deafness, autosomal recessive 39
  • DFNB39
  • EC 3.4.21
  • EC
  • fibroblast-derived tumor cytotoxic factor
  • F-TCF
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • Hepatopoeitin-A
  • Hepatopoietin A
  • HGF
  • HGFB
  • HPTA
  • HPTAhepatocyte growth factor
  • lung fibroblast-derived mitogen
  • Scatter factor
  • SF
  • SFhepatopoeitin-A


HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates human HGF isoforms that lack the proteinase‑like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH‑stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

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Publications for HGF (294-HG)(94)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Equine, Fish - Prionace glauca (Blue Shark), Hamster, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Squirrel.

We have publications tested in 7 applications: Activation, Bioassay, Cell Culture, Control, ELISA (Standard), In Vivo, Scaffold Fabrication.

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Cell Culture
ELISA (Standard)
In Vivo
Scaffold Fabrication
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Filter By Species
Fish - Prionace glauca (Blue Shark)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
All Species
Showing Publications 1 - 10 of 94. Show All 94 Publications.
Publications using 294-HG Applications Species
L Gesualdi, E Leonetti, A Cucina, BM Scicchitan, S Sorrentino, MG Tarsitano, A Isidori, M Bizzarri, A Filippini, A Riccioli, M Cammarota, V Gigantino, G Ricci, A Catizone The PI3K/AKT Pathway Is Activated by HGF in NT2D1 Non-Seminoma Cells and Has a Role in the Modulation of Their Malignant Behavior Int J Mol Sci, 2020;21(22):. 2020 [PMID: 33212946] (Cell Culture, Human) Cell Culture Human
AC Hedman, DE McNulty, Z Li, L Gorisse, RS Annan, DB Sacks Tyrosine phosphorylation of the scaffold protein IQGAP1 in the MET pathway alters function J Biol Chem, 2020;0(0):. 2020 [PMID: 33087447] (Bioassay, Mouse) Bioassay Mouse
A Walens, J Lin, JS Damrauer, B McKinney, R Lupo, R Newcomb, DB Fox, NW Mabe, J Gresham, Z Sheng, AB Sibley, T De Buyssch, H Kelkar, PA Mieczkowsk, K Owzar, JV Alvarez Adaptation and selection shape clonal evolution of tumors during residual disease and recurrence Nat Commun, 2020;11(1):5017. 2020 [PMID: 33024122] (Cell Culture, Human) Cell Culture Human
S Sato, N Matsumoto, K Hisaie, S Uematsu Alcohol abrogates human norovirus infectivity in a pH-dependent manner Sci Rep, 2020;10(1):15878. 2020 [PMID: 32985508] (Cell Culture, Human) Cell Culture Human
C Volz, S Breid, C Selenz, A Zaplatina, K Golfmann, L Meder, F Dietlein, S Borchmann, S Chatterjee, M Siobal, J Schöttle, A Florin, M Koker, M Nill, L Ozreti?, N Uhlenbrock, S Smith, R Büttner, H Miao, B Wang, HC Reinhardt, D Rauh, M Hallek, A Acker-Palm, LC Heukamp, RT Ullrich Inhibition of Tumor VEGFR2 Induces Serine 897 EphA2-Dependent Tumor Cell Invasion and Metastasis in NSCLC Cell Rep, 2020;31(4):107568. 2020 [PMID: 32348765] (Bioassay, Human) Bioassay Human
M Grosch, S Ittermann, E Rusha, T Greisle, C Ori, DJ Truong, AC O'Neill, A Pertek, GG Westmeyer, M Drukker Nucleus size and DNA accessibility are linked to the regulation of paraspeckle formation in cellular differentiation BMC Biol., 2020;18(1):42. 2020 [PMID: 32321486] (Cell Culture, Human) Cell Culture Human
K Han, SE Pierce, A Li, K Spees, GR Anderson, JA Seoane, YH Lo, M Dubreuil, M Olivas, RA Kamber, M Wainberg, K Kostyrko, MR Kelly, M Yousefi, SW Simpkins, D Yao, K Lee, CJ Kuo, PK Jackson, A Sweet-Cord, A Kundaje, AJ Gentles, C Curtis, MM Winslow, MC Bassik CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities Nature, 2020;580(7801):136-141. 2020 [PMID: 32238925] (Bioassay, Human) Bioassay Human
A Fumagalli, KC Oost, L Kester, J Morgner, L Bornes, L Bruens, L Spaargaren, M Azkanaz, T Schelfhors, E Beerling, MC Heinz, D Postrach, D Seinstra, AM Sieuwerts, JWM Martens, S van der El, M van Baalen, D Bhowmick, N Vrisekoop, SIJ Ellenbroek, SJE Suijkerbui, HJ Snippert, J van Rheene Plasticity of Lgr5-Negative Cancer Cells Drives Metastasis in Colorectal Cancer Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32169167] (Bioassay, Human) Bioassay Human
NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020;0(0):. 2020 [PMID: 32094658] (Cell Culture, Human) Cell Culture Human
JS Kim, SI Hwang, JL Ryu, HS Hong, JM Lee, SM Lee, X Jin, C Han, JH Kim, J Han, MR Lee, DH Woo ER stress reliever enhances functionalities of in vitro cultured hepatocytes Stem Cell Res, 2020;43(0):101732. 2020 [PMID: 32087526] (Human) Human
Show All 94 Publications.

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Gene Symbol HGF