CD31/PECAM-1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed ...read more
Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (AB-108-C, open histogram), followed by ...read more
Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by ...read more
CD31/PECAM-1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at room ...read more
CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 ...read more
CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 hour at ...read more
CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of rat heart using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at ...read more
CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

CD31/PECAM-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
Glu18-Lys590
Accession # Q08481
Specificity
Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PECAM1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 7 Reviews rated 5
using
AF3628 in the following applications:

Publications
Read Publications using
AF3628 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Unconjugated]

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • EndoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet endothelial cell adhesion molecule-1
  • platelet/endothelial cell adhesion molecule

Background

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

  1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
  2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
  3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
  4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
  5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
  6. Ilan, N. et al. (2001) FASEB J. 15:362.
  7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
  8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
  9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
  10. Mamdouh, Z. et al. (2003) Nature 421:748.
  11. Gao, C. et al. (2003) Blood 102:169.
  12. Falati, S. et al. (2006) Blood 107:535.
  13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
  14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (AF3628)(219)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Transgenic Mouse, Xenograft.

We have publications tested in 16 applications: Cell Culture, Cell Selection, Flow Cytometry, Functional Assay, ICC, ICC/IF, IF, IF/IHC, IHC, IHC fixed frozen, IHC-Fr, IHC-P, IHC-Pr, IHC/IF, Ifs, Western Blot.


Filter By Application
Cell Culture
(2)
Cell Selection
(1)
Flow Cytometry
(5)
Functional Assay
(1)
ICC
(8)
ICC/IF
(2)
IF
(2)
IF/IHC
(1)
IHC
(168)
IHC fixed frozen
(1)
IHC-Fr
(8)
IHC-P
(19)
IHC-Pr
(1)
IHC/IF
(3)
Ifs
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(10)
Mouse
(175)
Rat
(28)
Transgenic Mouse
(9)
Xenograft
(3)
All Species
Showing Publications 1 - 10 of 219. Show All 219 Publications.
Publications using AF3628 Applications Species
Ayasoufi, K;Wolf, DM;Namen, SL;Jin, F;Tritz, ZP;Pfaller, CK;Zheng, J;Goddery, EN;Fain, CE;Gulbicki, LR;Borchers, AL;Reesman, RA;Yokanovich, LT;Maynes, MA;Bamkole, MA;Khadka, RH;Hansen, MJ;Wu, LJ;Johnson, AJ; Brain resident memory T cells rapidly expand and initiate neuroinflammatory responses following CNS viral infection Brain, behavior, and immunity 2023-05-24 [PMID: 37236326] (IHC, Mouse) IHC Mouse
Li, X;Jiang, Q;Song, G;Barkestani, MN;Wang, Q;Wang, S;Fan, M;Fang, C;Jiang, B;Johnson, J;Geirsson, A;Tellides, G;Pober, JS;Jane-Wit, D; A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells Nature communications 2023-05-24 [PMID: 37225719] (IHC, Transgenic Mouse, Mouse, Human) IHC Transgenic Mouse, Mouse, Human
Huang, HC;Wang, TY;Rousseau, J;Mungaray, M;Michaud, C;Plaisier, C;Chen, ZB;Wang, KC; Lesion-specific suppression of YAP/TAZ by biomimetic nanodrug ameliorates atherosclerosis development bioRxiv : the preprint server for biology 2023-04-26 [PMID: 37163067] (IHC, Transgenic Mouse) IHC Transgenic Mouse
M Liu, J Huang, T Liu, J Yuan, C Lv, Z Sha, C Wu, W Jiang, X Liu, M Nie, Y Chen, S Dong, Y Qian, C Gao, Y Fan, D Wu, R Jiang Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice Acta neuropathologica communications, 2023-04-07;11(1):61. 2023-04-07 [PMID: 37024941] (IHC, Mouse) IHC Mouse
BJ Choi, MH Park, KH Park, WH Han, HJ Yoon, HY Jung, JY Hong, MR Chowdhury, KY Kim, J Lee, IS Song, M Pang, MK Choi, E Gulbins, M Reichel, J Kornhuber, CW Hong, C Kim, SH Kim, EH Schuchman, HK Jin, JS Bae Immunotherapy targeting plasma ASM is protective in a mouse model of Alzheimer&#039;s disease Nature Communications, 2023-03-24;14(1):1631. 2023-03-24 [PMID: 36959217] (IHC, Mouse) IHC Mouse
K Utagawa, T Shin, H Yamada, H Ochi, S Sunamura, A Unno, C Akazawa, M Ema, S Takeda, A Okawa, S Sato Three-dimensional visualization of neural networks inside bone by Osteo-DISCO protocol and alteration of bone remodeling by surgical nerve ablation Scientific Reports, 2023-03-22;13(1):4674. 2023-03-22 [PMID: 36949102] (IHC, Mouse) IHC Mouse
Q Yu, I Gamayun, P Wartenberg, Q Zhang, S Qiao, S Kusumakshi, S Candlish, V Götz, S Wen, D Das, A Wyatt, V Wahl, F Ectors, K Kattler, D Yildiz, V Prevot, M Schwaninge, G Ternier, P Giacobini, P Ciofi, TD Müller, U Boehm Bitter taste cells in the ventricular walls of the murine brain regulate glucose homeostasis Nature Communications, 2023-03-22;14(1):1588. 2023-03-22 [PMID: 36949050] (IHC, Mouse) IHC Mouse
TR Madaris, M Venkatesan, S Maity, MC Stein, N Vishnu, MK Venkateswa, JG Davis, K Ramachandr, S Uthayabala, C Allen, A Osidele, K Stanley, NP Bigham, TM Bakewell, M Narkunan, A Le, V Karanam, K Li, A Mhapankar, L Norton, J Ross, MI Aslam, WB Reeves, BB Singh, J Caplan, JJ Wilson, PB Stathopulo, JA Baur, M Madesh Limiting Mrs2-dependent mitochondrial Mg2+ uptake induces metabolic programming in prolonged dietary stress Cell Reports, 2023-02-27;42(3):112155. 2023-02-27 [PMID: 36857182] (IHC, Mouse) IHC Mouse
C Di Nitto, E Gilardoni, J Mock, L Nadal, T Weiss, M Weller, F Seehusen, C Libbra, E Puca, D Neri, R De Luca An Engineered IFNgamma-Antibody Fusion Protein with Improved Tumor-Homing Properties Pharmaceutics, 2023-01-22;15(2):. 2023-01-22 [PMID: 36839699] (IHC, Mouse) IHC Mouse
CS Sung, HJ Cheng, NF Chen, SH Tang, HM Kuo, PJ Sung, WF Chen, ZH Wen Antinociceptive Effects of Aaptamine, a Sponge Component, on Peripheral Neuropathy in Rats Marine Drugs, 2023-02-04;21(2):. 2023-02-04 [PMID: 36827154] (IHC, Rat) IHC Rat
Show All 219 Publications.

Reviews for CD31/PECAM-1 Antibody (AF3628) (7) 57

Average Rating: 5
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Mouse, Mouse and Mouse brain tissue.

Reviews using AF3628:
Filter by Applications
IHC-P
(1)
IHC
(1)
IHC-Fr
(2)
Simple Western
(1)
ICC
(2)
All Applications
Filter by Species
Mouse
(6)
Mouse and Mouse brain tissue
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Kevin Thorburn
IHC-P Mouse 04/06/2023
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFFPE
SpeciesMouse
LotYZU0122121
Immunohistochemistry CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Verified Customer
IHC Mouse and Mouse brain tissue 05/10/2019
View

Summary

ApplicationImmunohistochemistry
Sample TestedP12 brain cortex,P21 brain cortex
SpeciesMouse and Mouse brain tissue
LotYZU0117011

Comments

CommentsBlocking with 10% donkey serum and PBST (.3%TritonX100). Free floating vibratome sections of 80microns and staining for brain clearing purposes where methanol treatment was used, the antibody still worked really good.
Immunohistochemistry-Frozen CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 05/09/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult spinal cord,Embryonic spinal cord
SpeciesMouse
LotYZU00118051
Simple Western CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Thomas Korff
Simple Western Mouse 05/25/2018
View

Summary

ApplicationSimple Western
Sample TestedVein Lysate
SpeciesMouse
LotYZU0118011

Comments

CommentsThis run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50). Lysis buffer: RIPA, prot. conc.: 0.2 µg/µl. Simple Western lane view shows one specific signal (~135 kDa) in lysates prepared from a mouse vein.

Immunohistochemistry-Frozen CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Shachar Dagan
IHC-Fr Mouse 04/02/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain
SpeciesMouse
LotYZU0116121

Comments

CommentsCD31/PECAM‑1 was detected in PFA fixed brain sections of adult mouse using Goat Anti-Mouse/PECAM‑1 (#AF3628) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Alexa568-conjugated Donkey anti-Goat
IgG Secondary Antibody and counterstained with DAPI (blue).
Immunocytochemistry CD31/PECAM-1 AF3628
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5
reviewed by:
Verified Customer
ICC Mouse 05/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Liver Tissue
SpeciesMouse

Comments

CommentsFrozen Tissue
4% PFA fixation
Immunocytochemistry CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Verified Customer
ICC Mouse 05/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Liver Tissue
SpeciesMouse
LotYZU0116121

Comments

CommentsFrozen tissue
Cold Acetone fixation

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD31/PECAM-1 Antibody (AF3628). (Showing 1 - 1 of 1 FAQs).

  1. Does CD31/PECAM-1 antibody (AF3628) work on Rat Paraffin embedded tissues(FFPE)?
    • For this antibody AF3628, it has been validated for mouse and rat and listed are multiple publications stating it was used for IHC-Paraffin.

Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to CD31/PECAM-1 Antibody (AF3628). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD31/PECAM-1.


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The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Recent Reviews

5
5
7
4
0
3
0
2
0
1
0

Kevin Thorburn
04/06/2023
Application: IHC-P
Species: Mouse

Verified Customer
05/10/2019
Application: IHC
Species: Mouse and Mouse brain tissue

Verified Customer
05/09/2019
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol PECAM1
Uniprot