CD31/PECAM-1 Antibody [Unconjugated]

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108).

Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108).

CD31/PECAM-1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; NL003) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing guts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of rat heart using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in vasculature.

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in chorionic villi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD31/PECAM-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
  Glu18-Lys590
  Accession # Q08481
• Specificity: Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PECAM1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Unconjugated]

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM^-/- mice are deficient in chemokine-mediated chemotaxis (14).

1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
6. Ilan, N. et al. (2001) FASEB J. 15:362.
7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
10. Mamdouh, Z. et al. (2003) Nature 421:748.
11. Gao, C. et al. (2003) Blood 102:169.
12. Falati, S. et al. (2006) Blood 107:535.
13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD31/PECAM-1 Antibody (AF3628)(219)

Publications using AF3628

• Ayasoufi, K;Wolf, DM;Namen, SL;Jin, F;Tritz, ZP;Pfaller, CK;Zheng, J;Goddery, EN;Fain, CE;Gulbicki, LR;Borchers, AL;Reesman, RA;Yokanovich, LT;Maynes, MA;Bamkole, MA;Khadka, RH;Hansen, MJ;Wu, LJ;Johnson, AJ; Brain resident memory T cells rapidly expand and initiate neuroinflammatory responses following CNS viral infection Brain, behavior, and immunity 2023-05-24 [PMID: 37236326] (IHC, Mouse)
• Li, X;Jiang, Q;Song, G;Barkestani, MN;Wang, Q;Wang, S;Fan, M;Fang, C;Jiang, B;Johnson, J;Geirsson, A;Tellides, G;Pober, JS;Jane-Wit, D; A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells Nature communications 2023-05-24 [PMID: 37225719] (IHC, Transgenic Mouse, Mouse, Human)
• Huang, HC;Wang, TY;Rousseau, J;Mungaray, M;Michaud, C;Plaisier, C;Chen, ZB;Wang, KC; Lesion-specific suppression of YAP/TAZ by biomimetic nanodrug ameliorates atherosclerosis development bioRxiv : the preprint server for biology 2023-04-26 [PMID: 37163067] (IHC, Transgenic Mouse)
• M Liu, J Huang, T Liu, J Yuan, C Lv, Z Sha, C Wu, W Jiang, X Liu, M Nie, Y Chen, S Dong, Y Qian, C Gao, Y Fan, D Wu, R Jiang Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice Acta neuropathologica communications, 2023-04-07;11(1):61. 2023-04-07 [PMID: 37024941] (IHC, Mouse)
• BJ Choi, MH Park, KH Park, WH Han, HJ Yoon, HY Jung, JY Hong, MR Chowdhury, KY Kim, J Lee, IS Song, M Pang, MK Choi, E Gulbins, M Reichel, J Kornhuber, CW Hong, C Kim, SH Kim, EH Schuchman, HK Jin, JS Bae Immunotherapy targeting plasma ASM is protective in a mouse model of Alzheimer&#039;s disease Nature Communications, 2023-03-24;14(1):1631. 2023-03-24 [PMID: 36959217] (IHC, Mouse)
• K Utagawa, T Shin, H Yamada, H Ochi, S Sunamura, A Unno, C Akazawa, M Ema, S Takeda, A Okawa, S Sato Three-dimensional visualization of neural networks inside bone by Osteo-DISCO protocol and alteration of bone remodeling by surgical nerve ablation Scientific Reports, 2023-03-22;13(1):4674. 2023-03-22 [PMID: 36949102] (IHC, Mouse)
• Q Yu, I Gamayun, P Wartenberg, Q Zhang, S Qiao, S Kusumakshi, S Candlish, V Götz, S Wen, D Das, A Wyatt, V Wahl, F Ectors, K Kattler, D Yildiz, V Prevot, M Schwaninge, G Ternier, P Giacobini, P Ciofi, TD Müller, U Boehm Bitter taste cells in the ventricular walls of the murine brain regulate glucose homeostasis Nature Communications, 2023-03-22;14(1):1588. 2023-03-22 [PMID: 36949050] (IHC, Mouse)
• TR Madaris, M Venkatesan, S Maity, MC Stein, N Vishnu, MK Venkateswa, JG Davis, K Ramachandr, S Uthayabala, C Allen, A Osidele, K Stanley, NP Bigham, TM Bakewell, M Narkunan, A Le, V Karanam, K Li, A Mhapankar, L Norton, J Ross, MI Aslam, WB Reeves, BB Singh, J Caplan, JJ Wilson, PB Stathopulo, JA Baur, M Madesh Limiting Mrs2-dependent mitochondrial Mg2+ uptake induces metabolic programming in prolonged dietary stress Cell Reports, 2023-02-27;42(3):112155. 2023-02-27 [PMID: 36857182] (IHC, Mouse)
• C Di Nitto, E Gilardoni, J Mock, L Nadal, T Weiss, M Weller, F Seehusen, C Libbra, E Puca, D Neri, R De Luca An Engineered IFNgamma-Antibody Fusion Protein with Improved Tumor-Homing Properties Pharmaceutics, 2023-01-22;15(2):. 2023-01-22 [PMID: 36839699] (IHC, Mouse)
• CS Sung, HJ Cheng, NF Chen, SH Tang, HM Kuo, PJ Sung, WF Chen, ZH Wen Antinociceptive Effects of Aaptamine, a Sponge Component, on Peripheral Neuropathy in Rats Marine Drugs, 2023-02-04;21(2):. 2023-02-04 [PMID: 36827154] (IHC, Rat)
• W Song, Y Yang, P Heizer, Y Tu, TA Weston, JR Kim, P Munguia, H Jung, JL Fong, C Tran, M Ploug, AP Beigneux, SG Young, LG Fong Intracapillary LPL levels in brown adipose tissue, visualized with an antibody-based approach, are regulated by ANGPTL4 at thermoneutral temperatures Proceedings of the National Academy of Sciences of the United States of America, 2023-02-14;120(8):e2219833120. 2023-02-14 [PMID: 36787365] (Western Blot, Mouse)
• J Yu, Y Shen, J Luo, J Jin, P Li, P Feng, H Guan Upadacitinib inhibits corneal inflammation and neovascularization by suppressing M1 macrophage infiltration in the corneal alkali burn model International immunopharmacology, 2023-02-03;116(0):109680. 2023-02-03 [PMID: 36739832] (IHC, Mouse)
• Q Zhu, X Zhou, Y Zhang, D Ye, K Yu, W Cao, L Zhang, H Zheng, Z Sun, C Guo, X Hong, Y Zhu, Y Zhang, Y Xiao, TG Valencak, T Ren, D Ren White-light crosslinkable milk protein bioadhesive with ultrafast gelation for first-aid wound treatment Biomaterials research, 2023-02-03;27(1):6. 2023-02-03 [PMID: 36737833] (IHC, Mouse)
• G Wei, C Li, X Jia, J Xie, Z Tang, M Jin, Q Chen, Y Sun, S He, X Li, Y Chen, H Zheng, W Liao, Y Liao, J Bin, S Huang Extracellular vesicle-derived CircWhsc1 Promotes Cardiomyocyte Proliferation and Heart Repair by activating TRIM59/STAT3/Cyclin B2 pathway Journal of advanced research, 2022-12-29;0(0):. 2022-12-29 [PMID: 36587763] (ICC/IF, IHC/IF, Mouse)
• BM Silveira, TO Ribeiro, RS Freitas, ACO Carreira, MS Gonçalves, M Sogayar, R Meyer, A Birbrair, V Fortuna Secretome from human adipose-derived mesenchymal stem cells promotes blood vessel formation and pericyte coverage in experimental skin repair PLoS ONE, 2022-12-19;17(12):e0277863. 2022-12-19 [PMID: 36534643] (IHC, Mouse)
• NB McNamara, DAD Munro, N Bestard-Cu, A Uyeda, JFJ Bogie, A Hoffmann, RK Holloway, I Molina-Gon, KE Askew, S Mitchell, W Mungall, M Dodds, C Dittmayer, J Moss, J Rose, S Szymkowiak, L Amann, BW McColl, M Prinz, TL Spires-Jon, W Stenzel, K Horsburgh, JJA Hendriks, C Pridans, R Muramatsu, A Williams, J Priller, VE Miron Microglia regulate central nervous system myelin growth and integrity Nature, 2022-12-14;0(0):. 2022-12-14 [PMID: 36517604] (IHC, Mouse)
• NB McNamara, DAD Munro, N Bestard-Cu, A Uyeda, JFJ Bogie, A Hoffmann, RK Holloway, I Molina-Gon, KE Askew, S Mitchell, W Mungall, M Dodds, C Dittmayer, J Moss, J Rose, S Szymkowiak, L Amann, BW McColl, M Prinz, TL Spires-Jon, W Stenzel, K Horsburgh, JJA Hendriks, C Pridans, R Muramatsu, A Williams, J Priller, VE Miron Microglia regulate central nervous system myelin growth and integrity Nature, 2022;0(0):. 2022 [PMID: 36517604] (IHC, Mouse)
• Y Jin, H Kim, S Min, YS Choi, SJ Seo, E Jeong, SK Kim, HA Lee, SH Jo, JH Park, BW Park, WS Sim, JJ Kim, K Ban, YG Kim, HJ Park, SW Cho Three-dimensional heart extracellular matrix enhances chemically induced direct cardiac reprogramming Science Advances, 2022-12-14;8(50):eabn5768. 2022-12-14 [PMID: 36516259] (IHC, Rat)
• X Liang, W Fa, N Wang, Y Peng, C Liu, M Zhu, N Tian, Y Wang, X Han, C Qiu, T Hou, Y Du Exosomal miR-532-5p induced by long-term exercise rescues blood-brain barrier function in 5XFAD mice via downregulation of EPHA4 Aging Cell, 2022-12-09;0(0):e13748. 2022-12-09 [PMID: 36494892] (IHC, Mouse)
• C Lin, Q Yang, D Guo, J Xie, YS Yang, S Chaugule, N DeSouza, WT Oh, R Li, Z Chen, AA John, Q Qiu, LJ Zhu, MB Greenblatt, S Ghosh, S Li, G Gao, C Haynes, CP Emerson, JH Shim Impaired mitochondrial oxidative metabolism in skeletal progenitor cells leads to musculoskeletal disintegration Nature Communications, 2022-11-11;13(1):6869. 2022-11-11 [PMID: 36369293] (IHC, Mouse)
• C Niu, J Yu, T Zou, Y Lu, L Deng, H Yun, CY Si, X Wu, H Jiang, T Guo, M Wu, T Kan, J Feng, C Yuan, X Yang, Q Cheng, J Dong, Q Wang, J Zhang Identification of hematopoietic stem cells residing in the meninges of adult mice at steady state Cell Reports, 2022-11-08;41(6):111592. 2022-11-08 [PMID: 36351383] (IHC, Mouse)
• YM Morizawa, M Matsumoto, Y Nakashima, N Endo, T Aida, H Ishikane, K Beppu, S Moritoh, H Inada, N Osumi, E Shigetomi, S Koizumi, G Yang, H Hirai, K Tanaka, KF Tanaka, N Ohno, Y Fukazawa, K Matsui Synaptic pruning through glial synapse engulfment upon motor learning Nature Neuroscience, 2022-11-01;25(11):1458-1469. 2022-11-01 [PMID: 36319770] (IHC, Transgenic Mouse)
• W Zhang, Z Xu, X Hao, T He, J Li, Y Shen, K Liu, Y Gao, J Liu, D Edwards, AM Muscarella, L Wu, L Yu, L Xu, X Chen, YH Wu, IL Bado, Y Ding, S Aguirre, H Wang, Z Gugala, RL Satcher, ST Wong, XH Zhang Bone metastasis initiation is coupled with bone remodeling through osteogenic differentiation of NG2+ cells Cancer Discovery, 2023-02-06;0(0):. 2023-02-06 [PMID: 36287038] (IHC, Transgenic Mouse)
• NL Tran, LM Ferreira, B Alvarez-Mo, V Buttiglion, B Ferrini, P Zordan, A Monestirol, C Fagioli, E Bezzecchi, GM Scotti, A Esposito, R Leone, C Gnasso, A Brendolan, LG Guidotti, G Sitia Continuous sensing of IFNalpha by hepatic endothelial cells shapes a vascular antimetastatic barrier Elife, 2022-10-25;11(0):. 2022-10-25 [PMID: 36281643] (IHC, Mouse)
• Y Xiao, Q Feng, L Huang, X Meng, P Han, W Zhang, YJ Kang Copper promotes cardiac functional recovery via suppressing the transformation of fibroblasts to myofibroblasts in ischemia-infarcted monkey hearts The Journal of nutritional biochemistry, 2022-10-12;0(0):109180. 2022-10-12 [PMID: 36240958] (IHC, Mouse)
• AP Amini, JD Kirkpatric, CS Wang, AM Jaeger, S Su, S Naranjo, Q Zhong, CM Cabana, T Jacks, SN Bhatia Multiscale profiling of protease activity in cancer Nature Communications, 2022-10-03;13(1):5745. 2022-10-03 [PMID: 36192379] (IHC, Mouse)
• M Bohley, AE Dillinger, F Schweda, A Ohlmann, BM Braunger, ER Tamm, A Goepferich A single intravenous injection of cyclosporin A-loaded lipid nanocapsules prevents retinopathy of prematurity Science Advances, 2022-09-23;8(38):eabo6638. 2022-09-23 [PMID: 36149956] (IHC/IF, Mouse)
• J Sun, J Qiu, Q Yang, Q Ju, R Qu, X Wang, L Wu, L Xing Single-cell RNA sequencing reveals dysregulation of spinal cord cell types in a severe spinal muscular atrophy mouse model PloS Genetics, 2022-09-08;18(9):e1010392. 2022-09-08 [PMID: 36074806] (IHC fixed frozen, Mouse)
• KJ Smith, D Minns, BJ McHugh, RK Holloway, R O'Connor, A Williams, L Melrose, R McPherson, VE Miron, DJ Davidson, E Gwyer Find The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiation PloS Biology, 2022-08-26;20(8):e3001554. 2022-08-26 [PMID: 36026478] (IHC, Human)
• Y Shen, J Cui, S Zhang, Y Wang, J Wang, Y Su, D Xu, Y Liu, Y Guo, W Bai Temporal alteration of microglia to microinfarcts in rat brain induced by the vascular occlusion with fluorescent microspheres Frontiers in Cellular Neuroscience, 2022-08-03;16(0):956342. 2022-08-03 [PMID: 35990892] (IHC, Rat)
• A Rajesh, S Droho, JA Lavine Macrophages in close proximity to the vitreoretinal interface are potential biomarkers of inflammation during retinal vascular disease Journal of Neuroinflammation, 2022-08-08;19(1):203. 2022-08-08 [PMID: 35941655] (IHC, Mouse)
• T Seki, Y Yang, X Sun, S Lim, S Xie, Z Guo, W Xiong, M Kuroda, H Sakaue, K Hosaka, X Jing, M Yoshihara, L Qu, X Li, Y Chen, Y Cao Brown-fat-mediated tumour suppression by cold-altered global metabolism Nature, 2022-08-03;608(7922):421-428. 2022-08-03 [PMID: 35922508] (IHC, Mouse)
• L Jiang, S Liu, T Deng, Y Yang, Y Zhang Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma Oncology Letters, 2022-06-07;24(2):244. 2022-06-07 [PMID: 35761940] (IHC, Mouse)
• Y Liu, Z Wang, C Cao, Z Xu, J Lu, H Shen, X Li, H Li, J Wu, G Chen Aquaporin 4 Depolarization-Enhanced Transferrin Infiltration Leads to Neuronal Ferroptosis after Subarachnoid Hemorrhage in Mice Oncogene, 2022-06-17;2022(0):8808677. 2022-06-17 [PMID: 35761873] (IHC, Mouse)
• AN Brandebura, DR Kolson, EM Amick, J Ramadan, MC Kersting, RH Nichol, PS Holcomb, PH Mathers, P Stoilov, GA Spirou Transcriptional profiling reveals roles of intercellular Fgf9 signaling in astrocyte maturation and synaptic refinement during brainstem development The Journal of Biological Chemistry, 2022-06-23;0(0):102176. 2022-06-23 [PMID: 35753346] (IHC, Mouse)
• J Zhang, Z Pan, J Zhou, L Zhang, J Tang, S Gong, F Li, B Yu, Y Zhang, J Kou The myosin II inhibitor, blebbistatin, ameliorates pulmonary endothelial barrier dysfunction in acute lung injury inducedB19 by LPS via NMMHC IIA/Wnt5a/beta-catenin pathway Oncogene, 2022-06-15;0(0):116132. 2022-06-15 [PMID: 35716767] (IHC, Mouse)
• D Xia, S Lianoglou, T Sandmann, M Calvert, JH Suh, E Thomsen, J Dugas, ME Pizzo, SL DeVos, TK Earr, CC Lin, S Davis, C Ha, AW Leung, H Nguyen, R Chau, E Yulyanings, I Lopez, H Solanoy, ST Masoud, CC Liang, K Lin, G Astarita, N Khoury, JY Zuchero, RG Thorne, K Shen, S Miller, JJ Palop, D Garceau, M Sasner, JD Whitesell, JA Harris, S Hummel, J Gnörich, K Wind, L Kunze, A Zatcepin, M Brendel, M Willem, C Haass, D Barnett, TS Zimmer, AG Orr, K Scearce-Le, JW Lewcock, G Di Paolo, PE Sanchez Novel App knock-in mouse model shows key features of amyloid pathology and reveals profound metabolic dysregulation of microglia Molecular Neurodegeneration, 2022-06-11;17(1):41. 2022-06-11 [PMID: 35690868] (IHC, Mouse)
• G Huang, Z Cheng, A Hildebrand, C Wang, M Cimini, R Roy, AM Lucchese, C Benedict, V Mallaredy, A Magadum, D Joladarash, C Thej, C Gonzalez, M Trungcao, VNS Garikipati, JW Elrod, WJ Koch, R Kishore Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition Theranostics, 2022-05-21;12(9):4415-4430. 2022-05-21 [PMID: 35673580] (IHC, Mouse)
• Y Yamada, Y Zhong, S Miki, A Taura, TH Rabbitts The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis Scientific Reports, 2022-05-04;12(1):7226. 2022-05-04 [PMID: 35508511] (IHC, Transgenic Mouse)
• N Tisch, C Mogler, A Stojanovic, R Luck, EA Korhonen, A Ellerkmann, H Adler, M Singhal, G Schermann, L Erkert, JV Patankar, A Karakatsan, AL Scherr, Y Fuchs, A Cerwenka, S Wirtz, BC Köhler, HG Augustin, C Becker, T Schmidt, C Ruiz de Al Caspase-8 in endothelial cells maintains gut homeostasis and prevents small bowel inflammation in mice Embo Molecular Medicine, 2022-05-02;0(0):e14121. 2022-05-02 [PMID: 35491615] (IHC, Mouse)
• PY Bai, SQ Chen, DL Jia, LH Pan, CB Liu, J Liu, W Luo, Y Yang, MY Sun, NF Wan, WW Rong, AJ Sun, JB Ge Environmental eustress improves postinfarction cardiac repair via enhancing cardiac macrophage survival Science Advances, 2022-04-27;8(17):eabm3436. 2022-04-27 [PMID: 35476440] (Western Blot, Mouse)
• JH Kim, JK Nam, AR Kim, MS Park, HJ Lee, J Park, J Kim, YJ Lee 2-Methoxyestradiol Inhibits Radiation-Induced Skin Injuries International Journal of Molecular Sciences, 2022-04-10;23(8):. 2022-04-10 [PMID: 35456989] (IHC/IF, Mouse)
• Z Li, S Yu, Y Liu, X Hu, Y Li, Z Xiao, Y Chen, D Tian, X Xu, L Cheng, M Zheng, J Jing SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRbeta pathway Journal of Neuroinflammation, 2022-04-16;19(1):95. 2022-04-16 [PMID: 35429978] (IHC, Mouse)
• AS Kazerouni, DA Hormuth, T Davis, MJ Bloom, S Mounho, G Rahman, J Virostko, TE Yankeelov, AG Sorace Quantifying Tumor Heterogeneity via MRI Habitats to Characterize Microenvironmental Alterations in HER2+ Breast Cancer Cancers, 2022-04-06;14(7):. 2022-04-06 [PMID: 35406609] (IHC, Mouse)
• S Lin, S Yin, J Shi, G Yang, X Wen, W Zhang, M Zhou, X Jiang Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration Bioactive materials, 2022-03-24;18(0):116-127. 2022-03-24 [PMID: 35387176] (IHC, Mouse)
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Reviews for CD31/PECAM-1 Antibody (AF3628) (7)

Average Rating: 5

(Based on 7 reviews)

We have 7 reviews tested in 2 species: Mouse, Mouse and Mouse brain tissue.

reviewed by: Kevin Thorburn IHC-P Mouse 04/06/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: FFPE
• Species: Mouse
• Lot: YZU0122121

reviewed by: Verified Customer IHC Mouse and Mouse brain tissue 05/10/2019

Summary

• Application: Immunohistochemistry
• Sample Tested: P12 brain cortex,P21 brain cortex
• Species: Mouse and Mouse brain tissue
• Lot: YZU0117011

Comments

• Comments: Blocking with 10% donkey serum and PBST (.3%TritonX100). Free floating vibratome sections of 80microns and staining for brain clearing purposes where methanol treatment was used, the antibody still worked really good.

reviewed by: Verified Customer IHC-Fr Mouse 05/09/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult spinal cord,Embryonic spinal cord
• Species: Mouse
• Lot: YZU00118051

reviewed by: Thomas Korff Simple Western Mouse 05/25/2018

Summary

• Application: Simple Western
• Sample Tested: Vein Lysate
• Species: Mouse
• Lot: YZU0118011

Comments

• Comments: This run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50). Lysis buffer: RIPA, prot. conc.: 0.2 µg/µl. Simple Western lane view shows one specific signal (~135 kDa) in lysates prepared from a mouse vein.

reviewed by: Shachar Dagan IHC-Fr Mouse 04/02/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain
• Species: Mouse
• Lot: YZU0116121

Comments

• Comments: CD31/PECAM‑1 was detected in PFA fixed brain sections of adult mouse using Goat Anti-Mouse/PECAM‑1 (#AF3628) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Alexa568-conjugated Donkey anti-Goat
  IgG Secondary Antibody and counterstained with DAPI (blue).

reviewed by: Verified Customer ICC Mouse 05/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Liver Tissue
• Species: Mouse

Comments

• Comments: Frozen Tissue
  4% PFA fixation

reviewed by: Verified Customer ICC Mouse 05/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Liver Tissue
• Species: Mouse
• Lot: YZU0116121

Comments

• Comments: Frozen tissue
  Cold Acetone fixation

FAQs for CD31/PECAM-1 Antibody (AF3628). (Showing 1 - 1 of 1 FAQs).

1. Does CD31/PECAM-1 antibody (AF3628) work on Rat Paraffin embedded tissues(FFPE)?
   • For this antibody AF3628, it has been validated for mouse and rat and listed are multiple publications stating it was used for IHC-Paraffin.

Bioinformatics

• Gene Symbol: PECAM1
• Uniprot:
  • Q08481()