CD31/PECAM-1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) ...read more
CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at ...read more
Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), ...read more
CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL ...read more
Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), ...read more
CD31/PECAM‑1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

CD31/PECAM-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
Glu18-Lys590
Accession # Q08481
Specificity
Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PECAM1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 4 Reviews rated 5
using
AF3628 in the following applications:

Publications
Read Publications using
AF3628 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Unconjugated]

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule

Background

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

  1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
  2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
  3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
  4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
  5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
  6. Ilan, N. et al. (2001) FASEB J. 15:362.
  7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
  8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
  9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
  10. Mamdouh, Z. et al. (2003) Nature 421:748.
  11. Gao, C. et al. (2003) Blood 102:169.
  12. Falati, S. et al. (2006) Blood 107:535.
  13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
  14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (AF3628)(39)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 13 applications: Flow, ICC, IF, IHC, IHC Fresh, IHC Frozen, IHC Not Specified, IHC OCT-embedded, IHC Paraffin-embedded, IHC-F, IHC-OCT embedded, IHC-P, WB.


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Flow
(2)
ICC
(2)
IF
(1)
IHC
(19)
IHC Fresh
(1)
IHC Frozen
(2)
IHC Not Specified
(1)
IHC OCT-embedded
(3)
IHC Paraffin-embedded
(3)
IHC-F
(1)
IHC-OCT embedded
(1)
IHC-P
(2)
WB
(1)
All Applications
Filter By Species
Mouse
(31)
Rat
(8)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using AF3628 Applications Species
L Elia, P Kunderfran, P Carullo, M Vacchiano, FM Farina, IF Hall, S Mantero, C Panico, R Papait, G Condorelli, M Quintavall UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29558369] (IHC,, Mouse) IHC Mouse
SS Rayagiri, D Ranaldi, A Raven, NIF Mohamad Az, O Lefebvre, PS Zammit, AG Borycki Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal Nat Commun, 2018;9(1):1075. 2018 [PMID: 29540680] (IHC,, Mouse) IHC Mouse
B Gao, X Lin, H Jing, J Fan, C Ji, Q Jie, C Zheng, D Wang, X Xu, Y Hu, W Lu, Z Luo, L Yang Local delivery of tetramethylpyrazine eliminates the senescent phenotype of bone marrow mesenchymal stromal cells and creates an anti-inflammatory and angiogenic environment in aging mice Aging Cell, 2018;0(0):. 2018 [PMID: 29488314] (Flow,, Mouse) Flow Mouse
S Sorrentino, C Iaconetti, S De Rosa, A Polimeni, J Sabatino, C Gareri, F Passafaro, T Mancuso, L Tammè, C Mignogna, C Camastra, G Esposito, A Curcio, D Torella, C Indolfi Hindlimb Ischemia Impairs Endothelial Recovery and Increases Neointimal Proliferation in the Carotid Artery Sci Rep, 2018;8(1):761. 2018 [PMID: 29335599] (IHC-P,, Rat) IHC-P Rat
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC,, Mouse) IHC Mouse
N Falzone, NL Ackerman, LF Rosales, MA Bernal, X Liu, SG Peeters, MS Soto, A Corroyer-D, M Bernaudin, E Grimoin, O Touzani, NR Sibson, KA Vallis Dosimetric evaluation of radionuclides for VCAM-1-targeted radionuclide therapy of early brain metastases Theranostics, 2018;8(1):292-303. 2018 [PMID: 29290808] (IHC,, Mouse) IHC Mouse
S Ling, RZ Ni, Y Yuan, YQ Dang, QM Zhou, S Liang, F Guo, W Feng, Y Chen, K Ikeda, Y Yamori, JW Xu Natural compound bavachalcone promotes the differentiation of endothelial progenitor cells and neovascularization through the ROR?-erythropoietin-AMPK axis Oncotarget, 2017;8(49):86188-86205. 2017 [PMID: 29156787] (IHC-P,, Rat) IHC-P Rat
M Chen, X Lei, C Shi, M Huang, X Li, B Wu, Z Li, W Han, B Du, J Hu, Q Nie, W Mai, N Ma, N Xu, X Zhang, C Fan, A Hong, M Xia, L Luo, A Ma, H Li, Q Yu, H Chen, D Zhang, W Ye Pericyte-targeting prodrug overcomes tumor resistance to vascular disrupting agents J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28846068] (IHC,, Mouse) IHC Mouse
Y He, X Xu, T Zhu, M Tang, J Mei, Y Si Resident Arterial Cells and Circulating Bone Marrow-Derived Cells both Contribute to Intimal Hyperplasia in a Rat Allograft Carotid Transplantation Model Cell. Physiol. Biochem., 2017;42(4):1303-1312. 2017 [PMID: 28715799] (ICC,, Mouse) ICC Mouse
M Aoyagi-Sch, D Crippen-Ha, R Lawrence, J Vincelette, G Yogalingam, H Prill, BK Yip, B Baridon, C Vitelli, A Lee, O Gorostiza, EG Adintori, WC Minto, JL Van Vleet, B Yates, S Rigney, TM Christians, PMN Tiger, MJ Lo, J Holtzinger, PA Fitzpatric, JH LeBowitz, S Bullens, BE Crawford, S Bunting Clearance of Heparan Sulfate and Attenuation of CNS Pathology by Intracerebroventricular BMN 250 in Sanfilippo Type B Mice Mol Ther Methods Clin Dev, 2017;6(0):43-53. 2017 [PMID: 28664165] (IHC,, Mouse) IHC Mouse
Show All 39 Publications.

Reviews for CD31/PECAM-1 Antibody (AF3628) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Mouse.

Reviews using AF3628:
Filter by Applications
Simple Western
(1)
IHC-Fr
(1)
ICC
(2)
All Applications
Filter by Species
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
Simple Western CD31/PECAM-1 AF3628
Enlarge
reviewed by:
Anonymous
Simple Western Mouse 05/25/2018
View

Summary

ApplicationSimple Western
Sample TestedVein Lysate
SpeciesMouse
LotYZU0118011

Comments

CommentsThis run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50). Lysis buffer: RIPA, prot. conc.: 0.2 µg/µl. Simple Western lane view shows one specific signal (~135 kDa) in lysates prepared from a mouse vein.

Immunohistochemistry-Frozen CD31/PECAM-1 AF3628
Enlarge
reviewed by:
Anonymous
IHC-Fr Mouse 04/02/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Brain
SpeciesMouse
LotYZU0116121

Comments

CommentsCD31/PECAM‑1 was detected in PFA fixed brain sections of adult mouse using Goat Anti-Mouse/PECAM‑1 (#AF3628) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Alexa568-conjugated Donkey anti-Goat
IgG Secondary Antibody and counterstained with DAPI (blue).
Immunocytochemistry CD31/PECAM-1 AF3628
Enlarge
reviewed by:
Jan Martinek
ICC Mouse 05/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Liver Tissue
SpeciesMouse

Comments

CommentsFrozen Tissue
4% PFA fixation
Immunocytochemistry CD31/PECAM-1 AF3628
Enlarge
reviewed by:
Jan Martinek
ICC Mouse 05/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Liver Tissue
SpeciesMouse
LotYZU0116121

Comments

CommentsFrozen tissue
Cold Acetone fixation

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FAQs for CD31/PECAM-1 Antibody (AF3628) (0)

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Blogs on CD31/PECAM-1.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

Anonymous
05/25/2018
Application: Simple Western
Species: Mouse

Anonymous
04/02/2018
Application: IHC-Fr
Species: Mouse

Jan Martinek
05/26/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol PECAM1
Entrez
Uniprot