CD31/PECAM-1 Antibody [Unconjugated]

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing guts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

CD31/PECAM‑1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD31/PECAM-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
  Glu18-Lys590
  Accession # Q08481
• Specificity: Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
• Details of Functionality: ActivityAssaywCitation not found None None None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PECAM1
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Unconjugated]

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• endoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet/endothelial cell adhesion molecule

Background

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM^-/- mice are deficient in chemokine-mediated chemotaxis (14).

1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
6. Ilan, N. et al. (2001) FASEB J. 15:362.
7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
10. Mamdouh, Z. et al. (2003) Nature 421:748.
11. Gao, C. et al. (2003) Blood 102:169.
12. Falati, S. et al. (2006) Blood 107:535.
13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD31/PECAM-1 Antibody (AF3628)(44)

Publications using AF3628

• T Lind, R Lugano, AM Gustafson, M Norgård, A van Haerin, A Dimberg, H Melhus, SP Robertson, G Andersson Bones in human CYP26B1 deficiency and rats with hypervitaminosis A phenocopy Vegfa overexpression Bone Rep, 2018;9(0):27-36. 2018 [PMID: 30003121] (IHC,, Rat)
• C Chute, X Yang, K Meyer, N Yang, K O'Neil, I Kasza, K Eliceiri, C Alexander, A Friedl Syndecan-1 induction in lung microenvironment supports the establishment of breast tumor metastases Breast Cancer Res., 2018;20(1):66. 2018 [PMID: 29976229] (IHC,, Mouse)
• IS Zaitoun, U Cikla, D Zafer, E Udho, R Almomani, A Suscha, P Cengiz, CM Sorenson, N Sheibani Attenuation of Retinal Vascular Development in Neonatal Mice Subjected to Hypoxic-Ischemic Encephalopathy Sci Rep, 2018;0(1):9166. 2018 [PMID: 29907863] (IHC,, Mouse)
• Y Li, S Chang, W Li, G Tang, Y Ma, Y Liu, F Yuan, Z Zhang, GY Yang, Y Wang cxcl12-engineered endothelial progenitor cells enhance neurogenesis and angiogenesis after ischemic brain injury in mice Stem Cell Res Ther, 2018;9(1):139. 2018 [PMID: 29751775] (IHC,, Mouse)
• Z Cheng, L Wang, M Qu, H Liang, W Li, Y Li, L Deng, Z Zhang, GY Yang Mesenchymal stem cells attenuate blood-brain barrier leakage after cerebral ischemia in mice J Neuroinflammation, 2018;15(1):135. 2018 [PMID: 29724240] (IHC-F,, Mouse)
• L Elia, P Kunderfran, P Carullo, M Vacchiano, FM Farina, IF Hall, S Mantero, C Panico, R Papait, G Condorelli, M Quintavall UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29558369] (IHC,, Mouse)
• SS Rayagiri, D Ranaldi, A Raven, NIF Mohamad Az, O Lefebvre, PS Zammit, AG Borycki Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal Nat Commun, 2018;9(1):1075. 2018 [PMID: 29540680] (IHC,, Mouse)
• B Gao, X Lin, H Jing, J Fan, C Ji, Q Jie, C Zheng, D Wang, X Xu, Y Hu, W Lu, Z Luo, L Yang Local delivery of tetramethylpyrazine eliminates the senescent phenotype of bone marrow mesenchymal stromal cells and creates an anti-inflammatory and angiogenic environment in aging mice Aging Cell, 2018;0(0):. 2018 [PMID: 29488314] (Flow,, Mouse)
• S Sorrentino, C Iaconetti, S De Rosa, A Polimeni, J Sabatino, C Gareri, F Passafaro, T Mancuso, L Tammè, C Mignogna, C Camastra, G Esposito, A Curcio, D Torella, C Indolfi Hindlimb Ischemia Impairs Endothelial Recovery and Increases Neointimal Proliferation in the Carotid Artery Sci Rep, 2018;8(1):761. 2018 [PMID: 29335599] (IHC-P,, Rat)
• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC,, Mouse)
• N Falzone, NL Ackerman, LF Rosales, MA Bernal, X Liu, SG Peeters, MS Soto, A Corroyer-D, M Bernaudin, E Grimoin, O Touzani, NR Sibson, KA Vallis Dosimetric evaluation of radionuclides for VCAM-1-targeted radionuclide therapy of early brain metastases Theranostics, 2018;8(1):292-303. 2018 [PMID: 29290808] (IHC,, Mouse)
• S Ling, RZ Ni, Y Yuan, YQ Dang, QM Zhou, S Liang, F Guo, W Feng, Y Chen, K Ikeda, Y Yamori, JW Xu Natural compound bavachalcone promotes the differentiation of endothelial progenitor cells and neovascularization through the ROR?-erythropoietin-AMPK axis Oncotarget, 2017;8(49):86188-86205. 2017 [PMID: 29156787] (IHC-P,, Rat)
• M Chen, X Lei, C Shi, M Huang, X Li, B Wu, Z Li, W Han, B Du, J Hu, Q Nie, W Mai, N Ma, N Xu, X Zhang, C Fan, A Hong, M Xia, L Luo, A Ma, H Li, Q Yu, H Chen, D Zhang, W Ye Pericyte-targeting prodrug overcomes tumor resistance to vascular disrupting agents J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28846068] (IHC,, Mouse)
• Y He, X Xu, T Zhu, M Tang, J Mei, Y Si Resident Arterial Cells and Circulating Bone Marrow-Derived Cells both Contribute to Intimal Hyperplasia in a Rat Allograft Carotid Transplantation Model Cell. Physiol. Biochem., 2017;42(4):1303-1312. 2017 [PMID: 28715799] (ICC,, Mouse)
• M Aoyagi-Sch, D Crippen-Ha, R Lawrence, J Vincelette, G Yogalingam, H Prill, BK Yip, B Baridon, C Vitelli, A Lee, O Gorostiza, EG Adintori, WC Minto, JL Van Vleet, B Yates, S Rigney, TM Christians, PMN Tiger, MJ Lo, J Holtzinger, PA Fitzpatric, JH LeBowitz, S Bullens, BE Crawford, S Bunting Clearance of Heparan Sulfate and Attenuation of CNS Pathology by Intracerebroventricular BMN 250 in Sanfilippo Type B Mice Mol Ther Methods Clin Dev, 2017;6(0):43-53. 2017 [PMID: 28664165] (IHC,, Mouse)
• LI Al Asoom Coronary angiogenic effect of long-term administration of Nigella sativa BMC Complement Altern Med, 2017;17(1):308. 2017 [PMID: 28610577] (IHC,, Rat)
• Y Jin, L Muhl, M Burmakin, Y Wang, AC Duchez, C Betsholtz, HM Arthur, L Jakobsson Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling Nat. Cell Biol., 2017;0(0):. 2017 [PMID: 28530660] (IHC,, Mouse)
• WW Sugden, R Meissner, T Aegerter-W, R Tsaryk, EV Leonard, J Bussmann, MJ Hamm, W Herzog, Y Jin, L Jakobsson, C Denz, AF Siekmann Endoglin controls blood vessel diameter through endothelial cell shape changes in response to haemodynamic cues Nat. Cell Biol., 2017;0(0):. 2017 [PMID: 28530658] (IHC,, Mouse)
• K Gerl, D Steppan, M Fuchs, C Wagner, C Willam, A Kurtz, B Kurt Activation of Hypoxia Signaling in Stromal Progenitors Impairs Kidney Development Am. J. Pathol., 2017;0(0):. 2017 [PMID: 28527294] (IHC,, Mouse)
• RK Schneider, A Mullally, A Dugourd, F Peisker, R Hoogenboez, PMH Van Strien, EM Bindels, D Heckl, G Büsche, D Fleck, G Müller-New, J Wongboonsi, M Ventura Fe, VG Puelles, J Saez-Rodri, BL Ebert, BD Humphreys, R Kramann Gli1(+) Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28457748] (IHC OCT-embedded,, Mouse)
• T Lind, C Öhman, G Calounova, A Rasmusson, G Andersson, G Pejler, H Melhus Excessive dietary intake of vitamin A reduces skull bone thickness in mice PLoS ONE, 2017;12(4):e0176217. 2017 [PMID: 28426756] (IHC Paraffin-embedded,, Rat)
• DD Tsao, SG Wang, BD Lynn, JI Nagy Immunofluorescence reveals unusual patterns of labelling for connexin43 localized to calbindin-D28K-positive interstitial cells in the pineal gland Eur. J. Neurosci., 2017;0(0):. 2017 [PMID: 28394432] (IHC-F,, Rat)
• AM Rodriguez, DX Jin, AD Wolfe, MM Mikedis, L Wierenga, MP Hashmi, C Viebahn, KM Downs Brachyury drives formation of a distinct vascular branchpoint critical for fetal-placental arterial union in the mouse gastrula Dev. Biol., 2017;0(0):. 2017 [PMID: 28389228] (IHC,, Mouse)
• N Yang, W Zhang, T He, Y Xing Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy Exp. Eye Res, 2017;0(0):. 2017 [PMID: 28257831] (ICC,, Mouse)
• S Nakamura, H Osaka, SI Muramatsu, N Takino, M Ito, S Aoki, EF Jimbo, K Shimazaki, T Onaka, S Ohtsuki, T Terasaki, T Yamagata Gene therapy for a mouse model of glucose transporter-1 deficiency syndrome Mol Genet Metab Rep, 2017;10(0):67-74. 2017 [PMID: 28119822] (IHC,, Mouse)
• Miao Chen Impacts of femoral artery and vein excision versus femoral artery excision on the hindlimb ischemic model in CD-1 mice Microvasc. Res, 2016;110(0):48-55. 2016 [PMID: 27998712] (IHC,, Mouse)
• Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27493244] (IHC OCT-embedded,, Mouse)
• TLR2-mediated leukocyte trafficking to the developing brain J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27493242] (IHC Frozen,, Mouse)
• Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R Integrin beta1 controls VE-cadherin localization and blood vessel stability. Nat Commun, 2015;6(0):6429. 2015 [PMID: 25752958] (IHC,, Mouse)
• Shi X, Zirbes K, Rasmussen T, Ferdous A, Garry M, Koyano-Nakagawa N, Garry D The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression. J Biol Chem, 2015;290(15):9614-25. 2015 [PMID: 25694434] (IHC Not Specified,, Mouse)
• Herrnberger, Leonie, Hennig, Robert, Kremer, Werner, Hellerbrand, Claus, Goepferich, Achim, Kalbitzer, Hans Rob, Tamm, Ernst R Formation of fenestrae in murine liver sinusoids depends on plasmalemma vesicle-associated protein and is required for lipoprotein passage. PLoS ONE, 2014;9(12):e115005. 2014 [PMID: 25541982] (IHC Paraffin-embedded,, Mouse)
• Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis. Oncogene, 2015;34(29):3826-38. 2015 [PMID: 25263443] (IHC Fresh,, Mouse)
• Ding L, Cheng R, Hu Y, Takahashi Y, Jenkins A, Keech A, Humphries K, Gu X, Elliott M, Xia X, Ma J Peroxisome proliferator-activated receptor alpha protects capillary pericytes in the retina. Am J Pathol, 2014;184(10):2709-20. 2014 [PMID: 25108226] (IF,, Mouse)
• Shi, Xudong, Chen, Guojun, Guo, Lian-Wan, Si, Yi, Zhu, Men, Pilla, Srikanth, Liu, Bo, Gong, Shaoqin, Kent, K Craig Periadventitial application of rapamycin-loaded nanoparticles produces sustained inhibition of vascular restenosis. PLoS ONE, 2014;9(2):e89227. 2014 [PMID: 24586612] (IHC,, Rat)
• Akashi Y, Oda T, Ohara Y, Miyamoto R, Kurokawa T, Hashimoto S, Enomoto T, Yamada K, Satake M, Ohkohchi N Anticancer effects of gemcitabine are enhanced by co-administered iRGD peptide in murine pancreatic cancer models that overexpressed neuropilin-1. Br J Cancer, 2014;110(6):1481-7. 2014 [PMID: 24556620] (IHC OCT-embedded,, Mouse)
• Wu Y, Chen X, Zhou Q, He Q, Kang J, Zheng J, Wang K, Duan T ITE and TCDD differentially regulate the vascular remodeling of rat placenta via the activation of AhR. PLoS ONE, 2014;9(1):e86549. 2014 [PMID: 24475139] (IHC Paraffin-embedded,, Rat)
• Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies. Am J Physiol Endocrinol Metab, 2014;306(4):E443-56. 2014 [PMID: 24368670] (WB,, Rat)
• Adini, Irit, Ghosh, Kaustabh, Adini, Avner, Chi, Zai-Long, Yoshimura, Takeru, Benny, Ofra, Connor, Kip M, Rogers, Michael, Bazinet, Lauren, Birsner, Amy E, Bielenberg, Diane R, D'Amato, Robert J Melanocyte-secreted fibromodulin promotes an angiogenic microenvironment. J Clin Invest, 2014;124(1):425-36. 2014 [PMID: 24355922] (IHC,, Mouse)
• Zeng, L, He, X, Wang, Y, Tang, Y, Zheng, C, Cai, H, Liu, J, Wang, Y, Fu, Y, Yang, G-Y MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain. Gene Ther, 2014;21(1):37-43. 2014 [PMID: 24152581] (IHC-OCT embedded,, Mouse)
• Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-beta signaling pathways. J Cell Sci, 2013;126(0):1392-405. 2013 [PMID: 23418351] (Flow,, Mouse)
• Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells. Cancer Res, 2013;73(7):2333-44. 2013 [PMID: 23365134] (IHC,, Mouse)
• Mazzon C, Anselmo A, Cibella J, Soldani C, Destro A, Kim N, Roncalli M, Burden SJ, Dustin ML, Sarukhan A, Viola A The critical role of agrin in the hematopoietic stem cell niche. Blood, 2011;118(10):2733-42. 2011 [PMID: 21653324] (IHC Frozen,, Mouse)
• Funa NS, Kriz V, Zang G, Calounova G, Akerblom B, Mares J, Larsson E, Sun Y, Betsholtz C, Welsh M Dysfunctional microvasculature as a consequence of shb gene inactivation causes impaired tumor growth. Cancer Res., 2009;69(5):2141-8. 2009 [PMID: 19223532] (IHC,, Mouse)
• Rebholz B, Haase I, Eckelt B, Paxian S, Flaig MJ, Ghoreschi K, Nedospasov SA, Mailhammer R, Debey-Pascher S, Schultze JL, Weindl G, Forster I, Huss R, Stratis A, Ruzicka T, Rocken M, Pfeffer K, Schmid RM, Rupec RA Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin. Immunity, 2007;27(2):296-307. 2007 [PMID: 17692539] (IHC,, Mouse)

Reviews for CD31/PECAM-1 Antibody (AF3628) (4)

Average Rating: 5

(Based on 4 reviews)

We have 4 reviews tested in 1 species: Mouse.

reviewed by: Anonymous Simple Western Mouse 05/25/2018

Summary

• Application: Simple Western
• Sample Tested: Vein Lysate
• Species: Mouse
• Lot: YZU0118011

Comments

• Comments: This run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50). Lysis buffer: RIPA, prot. conc.: 0.2 µg/µl. Simple Western lane view shows one specific signal (~135 kDa) in lysates prepared from a mouse vein.

reviewed by: Anonymous IHC-Fr Mouse 04/02/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain
• Species: Mouse
• Lot: YZU0116121

Comments

• Comments: CD31/PECAM‑1 was detected in PFA fixed brain sections of adult mouse using Goat Anti-Mouse/PECAM‑1 (#AF3628) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Alexa568-conjugated Donkey anti-Goat
  IgG Secondary Antibody and counterstained with DAPI (blue).

reviewed by: Jan Martinek ICC Mouse 05/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Liver Tissue
• Species: Mouse

Comments

• Comments: Frozen Tissue
  4% PFA fixation

reviewed by: Jan Martinek ICC Mouse 05/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Liver Tissue
• Species: Mouse
• Lot: YZU0116121

Comments

• Comments: Frozen tissue
  Cold Acetone fixation

Bioinformatics

• Gene Symbol: PECAM1
• Entrez:
  • 5175(Human)
  • 18613(Mouse)
  • 29583(Rat)
• Uniprot:
  • Q08481()