CD31/PECAM-1 Antibody [Unconjugated]

CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing guts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CD31/PECAM‑1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of rat heart using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in vasculature.

• Reactivity: Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD31/PECAM-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
  Glu18-Lys590
  Accession # Q08481
• Specificity: Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PECAM1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Unconjugated]

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM^-/- mice are deficient in chemokine-mediated chemotaxis (14).

1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
6. Ilan, N. et al. (2001) FASEB J. 15:362.
7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
10. Mamdouh, Z. et al. (2003) Nature 421:748.
11. Gao, C. et al. (2003) Blood 102:169.
12. Falati, S. et al. (2006) Blood 107:535.
13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD31/PECAM-1 Antibody (AF3628)(169)

Publications using AF3628

• JH Kim, JK Nam, AR Kim, MS Park, HJ Lee, J Park, J Kim, YJ Lee 2-Methoxyestradiol Inhibits Radiation-Induced Skin Injuries International Journal of Molecular Sciences, 2022;23(8):. 2022 [PMID: 35456989] (IHC/IF, Mouse)
• X Wen, Y Peng, M Gao, Y Zhu, Y Zhu, F Yu, T Zhou, J Shao, L Feng, X Ma Endothelial Transient Receptor Potential Canonical Channel Regulates Angiogenesis and Promotes Recovery After Myocardial Infarction Journal of the American Heart Association, 2022;11(6):e023678. 2022 [PMID: 35253458] (ICC/IF, Mouse)
• HB Liang, ZH Lai, XQ Tu, KQ Ding, JR He, GY Yang, H Sheng, LL Zeng MicroRNA-140-5p exacerbates vascular cognitive impairment by inhibiting neurogenesis in the adult mouse hippocampus after global cerebral ischemia Brain research bulletin, 2022;183(0):73-83. 2022 [PMID: 35248684] (IHC, Mouse)
• S Gao, L Zhou, J Lu, Y Fang, H Wu, W Xu, Y Pan, J Wang, X Wang, J Zhang, A Shao Cepharanthine Attenuates Early Brain Injury after Subarachnoid Hemorrhage in Mice via Inhibiting 15-Lipoxygenase-1-Mediated Microglia and Endothelial Cell Ferroptosis Oxidative Medicine and Cellular Longevity, 2022;2022(0):4295208. 2022 [PMID: 35186185] (IF, Mouse)
• X Li, X Gao, W Zhang, M Liu, Z Han, M Li, P Lei, Q Liu Microglial replacement in the aged brain restricts neuroinflammation following intracerebral hemorrhage Cell Death & Disease, 2022;13(1):33. 2022 [PMID: 35013119] (IHC, Mouse)
• Y Luo, F Yao, X Hu, Y Li, Y Chen, Z Li, Z Zhu, S Yu, D Tian, L Cheng, M Zheng, J Jing M1 macrophages impair tight junctions between endothelial cells after spinal cord injury Brain research bulletin, 2022;0(0):. 2022 [PMID: 34995751] (IHC, Mouse)
• Q Lu, J Xiong, Y Yuan, Z Ruan, Y Zhang, B Chai, L Li, S Cai, J Xiao, Y Wu, P Huang, H Zhang Minocycline improves the functional recovery after traumatic brain injury via inhibition of aquaporin-4 International journal of biological sciences, 2022;18(1):441-458. 2022 [PMID: 34975343] (IHC, Mouse)
• SH Lee, MG Rezzonico, BA Friedman, MH Huntley, WJ Meilandt, S Pandey, YJ Chen, A Easton, Z Modrusan, DV Hansen, M Sheng, CJ Bohlen TREM2-independent oligodendrocyte, astrocyte, and T�cell responses to tau and amyloid pathology in mouse models of Alzheimer disease Cell Reports, 2021;37(13):110158. 2021 [PMID: 34965428] (IHC, Transgenic Mouse)
• XF Qin, YG Shan, JH Gao, FX Li, YX Guo E3 ubiquitin ligase mind bomb 1 overexpression reduces apoptosis and inflammation of cardiac microvascular endothelial cells in coronary microvascular dysfunction Cellular Signalling, 2021;91(0):110223. 2021 [PMID: 34954392] (Flow Cytometry, Rat)
• E Azzoni, V Frontera, G Anselmi, C Rode, C James, EM Deltcheva, AS Demian, J Brown, C Barone, A Patelli, JR Harman, M Nicholls, SJ Conway, E Morrissey, SEW Jacobsen, DB Sparrow, AL Harris, T Enver, MFTR de Bruijn The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition Cell Reports, 2021;37(11):110103. 2021 [PMID: 34910918] (IHC, Mouse)
• J Taranda, G Mathew, K Watrud, N El-Amine, MF Lee, C Elowsky, A Bludova, S Escobar Av, DG Nowak, TL Wee, JE Wilkinson, LC Trotman, P Osten Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases Cell Reports, 2021;37(7):110027. 2021 [PMID: 34788609] (IHC, Mouse)
• S Heino, S Fang, M Lähde, J Högström, S Nassiri, A Campbell, D Flanagan, A Raven, M Hodder, N Nasreddin, HH Xue, M Delorenzi, S Leedham, TV Petrova, O Sansom, K Alitalo Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas Science Advances, 2021;7(47):eabj0512. 2021 [PMID: 34788095] (IHC, Mouse)
• A Heinrich, B Bhandary, SJ Potter, N Ratner, T DeFalco Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis Cell Reports, 2021;37(4):109885. 2021 [PMID: 34706238] (IHC, Mouse)
• Z Zhang, H Na, Q Gan, Q Tao, Y Alekseyev, J Hu, Z Yan, JB Yang, H Tian, S Zhu, Q Li, IM Rajab, JK Blusztajn, B Wolozin, A Emili, X Zhang, T Stein, LA Potempa, WQ Qiu Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer&#039;s disease? Aging Cell, 2021;0(0):e13501. 2021 [PMID: 34687487] (Western Blot, Mouse)
• C Agostinis, M Spazzapan, R Vuerich, A Balduit, C Stocco, A Mangogna, G Ricci, G Papa, S Zacchigna, R Bulla Differential Capability of Clinically Employed Dermal Regeneration Scaffolds to Support Vascularization for Tissue Bioengineering Biomedicines, 2021;9(10):. 2021 [PMID: 34680575] (Cell Culture, Human)
• JK Nam, JH Kim, MS Park, EH Kim, J Kim, YJ Lee Radiation-Induced Fibrotic Tumor Microenvironment Regulates Anti-Tumor Immune Response Cancers, 2021;13(20):. 2021 [PMID: 34680381] (ICC, Mouse)
• BR Thomson, P Liu, T Onay, J Du, SW Tompson, S Misener, RR Purohit, TL Young, J Jin, SE Quaggin Cellular crosstalk regulates the aqueous humor outflow pathway and provides new targets for glaucoma therapies Nature Communications, 2021;12(1):6072. 2021 [PMID: 34663817] (IHC, Human)
• M Kaduri, M Sela, S Kagan, M Poley, H Abumanhal-, P Mora-Raimu, A Ouro, N Dahan, D Hershkovit, J Shklover, J Shainsky-R, Y Buganim, A Schroeder Targeting neurons in the tumor microenvironment with bupivacaine nanoparticles reduces breast cancer progression and metastases Science Advances, 2021;7(41):eabj5435. 2021 [PMID: 34613777] (IHC, Mouse)
• ERM Zunica, S Yang, A Coulter, C White, JP Kirwan, LA Gilmore Moringa Oleifera Seed Extract Concomitantly Supplemented with Chemotherapy Worsens Tumor Progression in Mice with Triple Negative Breast Cancer and Obesity Nutrients, 2021;13(9):. 2021 [PMID: 34578801] (IHC, Xenograft)
• M Mesnieres, AM Böhm, N Peredo, D Trompet, R Valle-Tenn, M Bajaj, N Corthout, E Nefyodova, R Cardoen, P Baatsen, S Munck, A Nagy, JJ Haigh, S Khurana, CM Verfaillie, C Maes Fetal hematopoietic stem cell homing is controlled by VEGF regulating the integrity and oxidative status of the stromal-vascular bone marrow niches Cell Reports, 2021;36(8):109618. 2021 [PMID: 34433017] (IHC, Mouse)
• R Luck, A Karakatsan, B Shah, G Schermann, H Adler, J Kupke, N Tisch, HW Jeong, MK Back, F Hetsch, A D'Errico, M De Palma, E Wiedtke, D Grimm, A Acker-Palm, J von Engelh, RH Adams, HG Augustin, C Ruiz de Al The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner Cell Reports, 2021;36(7):109522. 2021 [PMID: 34407407] (IHC, Mouse)
• H Suzuki, H Iwamoto, M Nakano, T Nakamura, A Masuda, T Sakaue, T Tanaka, D Nakano, R Kuromatsu, T Niizeki, S Okamura, S Shimose, T Shirono, Y Noda, N Kamachi, H Yano, A Kawaguchi, H Koga, T Torimura Efficacy and tolerability of Sorafenib plus metronomic chemotherapy S-1 for advanced hepatocellular carcinoma in preclinical and clinical assessments Translational Oncology, 2021;14(11):101201. 2021 [PMID: 34388691] (IHC, Mouse)
• KK Sivaraj, HW Jeong, B Dharmaling, D Zeuschner, S Adams, M Potente, RH Adams Regional specialization and fate specification of bone stromal cells in skeletal development Cell Reports, 2021;36(2):109352. 2021 [PMID: 34260921] (IHC, Mouse)
• D Schafflick, J Wolbert, M Heming, C Thomas, M Hartlehner, AL Börsch, A Ricci, S Martín-Sal, X Li, IN Lu, M Pawlak, J Minnerup, JK Strecker, T Seidenbech, SG Meuth, A Hidalgo, A Liesz, H Wiendl, G Meyer Zu H Single-cell profiling of CNS border compartment leukocytes reveals that B cells and their progenitors reside in non-diseased meninges Nature Neuroscience, 2021;0(0):. 2021 [PMID: 34253922] (IHC, Mouse)
• M Richards, S Pal, E Sjöberg, P Martinsson, L Venkatrama, L Claesson-W Intra-vessel heterogeneity establishes enhanced sites of macromolecular leakage downstream of laminin &amp;alpha5 Cell Reports, 2021;35(12):109268. 2021 [PMID: 34161758] (IHC, Mouse)
• NA Blessing, Z Wu, SM Madhavan, JW Choy, M Chen, MK Shin, M Hoek, JR Sedor, JF O'Toole, LA Bruggeman Lack of APOL1 in proximal tubules of normal human kidneys and proteinuric APOL1 transgenic mouse kidneys PLoS ONE, 2021;16(6):e0253197. 2021 [PMID: 34138902] (IHC, Mouse)
• XM Zhang, LN Zeng, WY Yang, L Ding, KZ Chen, WJ Fu, SQ Zeng, YR Liang, GH Chen, HF Wu Inhibition of LncRNA Vof-16 expression promotes nerve regeneration and functional recovery after spinal cord injury Neural regeneration research, 2022;17(1):217-227. 2022 [PMID: 34100459] (IHC, Rat)
• JK Nam, AR Kim, SH Choi, JH Kim, KJ Choi, S Cho, JW Lee, HJ Cho, YW Kwon, J Cho, KS Kim, J Kim, HJ Lee, TS Lee, S Bae, HJ Hong, YJ Lee An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage Nature Communications, 2021;12(1):3279. 2021 [PMID: 34078883] (IHC, Mouse)
• Z Cheng, V Naga Srika, MM Truongcao, M Cimini, G Huang, C Wang, C Benedict, C Gonzalez, V Mallaredy, DA Goukassian, SK Verma, R Kishore Serum-Derived Small Extracellular Vesicles From Diabetic Mice Impair Angiogenic Property of Microvascular Endothelial Cells: Role of EZH2 Journal of the American Heart Association, 2021;10(10):e019755. 2021 [PMID: 33988033] (IHC, Mouse)
• H Li, Z Wang, Z Chen, T Ci, G Chen, D Wen, R Li, J Wang, H Meng, R Bryan Bell, Z Gu, G Dotti, Z Gu Disrupting tumour vasculature and recruitment of aPDL1-loaded platelets control tumour metastasis Nature Communications, 2021;12(1):2773. 2021 [PMID: 33986264] (Flow Cytometry, Mouse)
• X Wang, L Chung, J Hooks, DR Maestas, A Lebid, JI Andorko, L Huleihel, AF Chin, M Wolf, NT Remlinger, MA Stepp, F Housseau, JH Elisseeff Type 2 immunity induced by bladder extracellular matrix enhances corneal wound healing Science Advances, 2021;7(16):. 2021 [PMID: 33863719] (IHC, Mouse)
• Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke Microglia use TAM receptors to detect and engulf amyloid &amp;beta plaques Nature Immunology, 2021;0(0):. 2021 [PMID: 33859405] (IHC, Mouse)
• KP Kim, C Li, D Bunina, HW Jeong, J Ghelman, J Yoon, B Shin, H Park, DW Han, JB Zaugg, J Kim, T Kuhlmann, RH Adams, KM Noh, SA Goldman, HR Schöler Donor cell memory confers a metastable state of directly converted cells Cell Stem Cell, 2021;0(0):. 2021 [PMID: 33848472] (ICC, Mouse)
• VFED Smolders, K Lodder, C Rodríguez, O Tura-Ceide, JA Barberà, JW Jukema, PHA Quax, MJ Goumans, K Kurakula The Inflammatory Profile of CTEPH-Derived Endothelial Cells Is a Possible Driver of Disease Progression Cells, 2021;10(4):. 2021 [PMID: 33810533] (IHC, Human)
• S Sugimoto, E Kobayashi, M Fujii, Y Ohta, K Arai, M Matano, K Ishikawa, K Miyamoto, K Toshimitsu, S Takahashi, K Nanki, Y Hakamata, T Kanai, T Sato An organoid-based organ-repurposing approach to treat short bowel syndrome Nature, 2021;0(0):. 2021 [PMID: 33627870] (IHC, Rat)
• E Jung, M Osswald, M Ratliff, H Dogan, R Xie, S Weil, DC Hoffmann, FT Kurz, T Kessler, S Heiland, A von Deimli, F Sahm, W Wick, F Winkler Tumor cell plasticity, heterogeneity, and resistance in crucial microenvironmental niches in glioma Nature Communications, 2021;12(1):1014. 2021 [PMID: 33579922] (IHC, Mouse)
• PH Lai, TH Wang, NY Zhang, KC Wu, CJ Yao, CJ Lin Changes of blood-brain-barrier function and transfer of amyloid beta in rats with collagen-induced arthritis Journal of Neuroinflammation, 2021;18(1):35. 2021 [PMID: 33516259] (IHC, Rat)
• M Mamtilahun, Z Wei, C Qin, Y Wang, Y Tang, FX Shen, HL Tian, Z Zhang, GY Yang DL-3n-Butylphthalide Improves Blood-Brain Barrier Integrity in Rat After Middle Cerebral Artery Occlusion Frontiers in Cellular Neuroscience, 2020;14(0):610714. 2020 [PMID: 33510620] (IHC, Rat)
• H Wang, Y Liang, Y Yin, J Zhang, W Su, AM White, Bin Jiang, J Xu, Y Zhang, S Stewart, X Lu, X He Carbon nano-onion-mediated dual targeting of P-selectin and P-glycoprotein to overcome cancer drug resistance Nature Communications, 2021;12(1):312. 2021 [PMID: 33436622] (IHC, Mouse)
• MR Eber, SH Park, KF Contino, CM Patel, FC Hsu, Y Shiozawa Osteoblasts derived from mouse mandible enhance tumor growth of prostate cancer more than osteoblasts derived from long bone Journal of Bone Oncology, 2021;26(0):100346. 2021 [PMID: 33425674] (IHC, Mouse)
• MM Gladka, A Kohela, B Molenaar, D Versteeg, L Kooijman, J Monshouwer, V Kremer, HR Vos, MMH Huibers, JJ Haigh, D Huylebroec, RA Boon, M Giacca, E van Rooij Cardiomyocytes stimulate angiogenesis after ischemic injury in a ZEB2-dependent manner Nature Communications, 2021;12(1):84. 2021 [PMID: 33398012] (IHC, Mouse)
• BS Xie, X Wang, YH Pan, G Jiang, JF Feng, Y Lin Apolipoprotein E, low-density lipoprotein receptor, and immune cells control blood-brain barrier penetration by AAV-PHP.eB in mice Theranostics, 2021;11(3):1177-1191. 2021 [PMID: 33391529] (IHC, Mouse)
• B Dechaumet, D Cleret, MT Linossier, A Vanden-Bos, S Chanon, E Lefai, N Laroche, MH Lafage-Pro, L Vico Hypergravity as a gravitational therapy mitigates the effects of knee osteoarthritis on the musculoskeletal system in a murine model PLoS ONE, 2020;15(12):e0243098. 2020 [PMID: 33296408] (IHC, Mouse)
• Y Wang, Y Mi, J Tian, X Qiao, X Su, J Kang, Z Wu, G Wang, X Zhou, Y Zhou, R Li Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats Drug Des Devel Ther, 2020;14(0):4825-4834. 2020 [PMID: 33204068] (IHC, Rat)
• Y Xiong, MJ Scerbo, A Seelig, F Volta, N O'Brien, A Dicker, D Padula, H Lickert, JM Gerdes, PO Berggren Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling Elife, 2020;9(0):. 2020 [PMID: 33200981] (IHC, Mouse)
• MM Castillo, Q Yang, AS Sigala, DT McKinney, M Zhan, KL Chen, JA Jarzembows, R Sood The endothelial protein C receptor plays an essential role in the maintenance of pregnancy Sci Adv, 2020;6(45):. 2020 [PMID: 33158859] (IHC, Mouse)
• J Sun, H Feng, W Xing, Y Han, J Suo, AR Yallowitz, N Qian, Y Shi, MB Greenblatt, W Zou Histone demethylase LSD1 is critical for endochondral ossification during bone fracture healing Sci Adv, 2020;6(45):. 2020 [PMID: 33148658] (IHC-P, Mouse)
• S Nakamura, H Osaka, SI Muramatsu, N Takino, M Ito, EF Jimbo, C Watanabe, S Hishikawa, T Nakajima, T Yamagata Intra-cisterna magna delivery of an AAV vector with the GLUT1 promoter in a pig recapitulates the physiological expression of SLC2A1 Gene Ther, 2020;0(0):. 2020 [PMID: 33077933] (IHC, Human)
• Y Li, X He, R Kawaguchi, Y Zhang, Q Wang, A Monavarfes, Z Yang, B Chen, Z Shi, H Meng, S Zhou, J Zhu, A Jacobi, V Swarup, PG Popovich, DH Geschwind, Z He Microglia-organized scar-free spinal cord repair in neonatal mice Nature, 2020;0(0):. 2020 [PMID: 33029008] (IHC, Mouse)
• D Furutama, S Matsuda, Y Yamawaki, S Hatano, A Okanobu, T Memida, H Oue, T Fujita, K Ouhara, M Kajiya, N Mizuno, T Kanematsu, K Tsuga, H Kurihara IL-6 Induced by Periodontal Inflammation Causes Neuroinflammation and Disrupts the Blood-Brain Barrier Brain Sci, 2020;10(10):. 2020 [PMID: 32992470] (IHC, Mouse)
• K Greer, EKG Basso, C Kelly, A Cash, E Kowalski, S Cerna, CT Ocampo, X Wang, MH Theus Abrogation of atypical neurogenesis and vascular-derived EphA4 prevents repeated mild TBI-induced learning and memory impairments Scientific Reports, 2020;10(1):15374. 2020 [PMID: 32958852] (IHC, Mouse)
• A Julien, S Perrin, O Duchamp de, C Carvalho, M Bensidhoum, L Legeai-Mal, C Colnot FGFR3 in Periosteal Cells Drives Cartilage-to-Bone Transformation in Bone Repair Stem Cell Reports, 2020;0(0):. 2020 [PMID: 32916123] (IHC, Mouse)
• C Donato, L Kunz, F Castro-Gin, A Paasinen-S, K Strittmatt, BM Szczerba, R Scherrer, N Di Maggio, W Heusermann, O Biehlmaier, C Beisel, M Vetter, C Rochlitz, WP Weber, A Banfi, T Schroeder, N Aceto Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell Rep, 2020;32(10):108105. 2020 [PMID: 32905777] (IHC, Mouse)
• YY Fan, YJ Wang, J Guo, MN Wu, MS Zhang, BL Niu, Y Li, J Zhao, CH Yang, Y Li, M Chen, XY Jiao Delayed metformin treatment improves functional recovery following traumatic brain injury via central AMPK-dependent brain tissue repair Brain Res. Bull., 2020;164(0):146-156. 2020 [PMID: 32858129] (IHC, Mouse)
• J Li, K Zhang, Q Zhang, X Zhou, L Wen, J Ma, L Niu, C Li PPAR-&amp;gamma Mediates Ta-VNS-Induced Angiogenesis and Subsequent Functional Recovery after Experimental Stroke in Rats Biomed Res Int, 2020;2020(0):8163789. 2020 [PMID: 32775443] (IHC, Rat)
• K Hosaka, Y Yang, T Seki, Q Du, X Jing, X He, J Wu, Y Zhang, H Morikawa, M Nakamura, M Scherzer, X Sun, Y Xu, T Cheng, X Li, X Liu, Q Li, Y Liu, A Hong, Y Chen, Y Cao Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors Nat Commun, 2020;11(1):3704. 2020 [PMID: 32709869] (IHC, Mouse)
• C Rocha-Rese, W Yang, W Li, D Kreisel, L Adamo, DL Mann Developmental changes in myocardial B cells mirror changes in B cells associated with different organs JCI Insight, 2020;5(16):. 2020 [PMID: 32663200] (IHC, Mouse)
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Reviews for CD31/PECAM-1 Antibody (AF3628) (6)

Average Rating: 5

(Based on 6 reviews)

We have 6 reviews tested in 2 species: Mouse, Mouse and Mouse brain tissue.

reviewed by: Verified Customer Free floating vibratome sections of 80microns and staining for brain clearing purposes where methanol treatment was used, the antibody still worked really good. Mouse and Mouse brain tissue 05/10/2019

Summary

• Application: Other
• Sample Tested: P12 brain cortex,P21 brain cortex
• Species: Mouse and Mouse brain tissue
• Lot: YZU0117011

Comments

• Comments: Blocking with 10%donkey serum and PBST &#40;.3%TritonX100&#41;

reviewed by: Verified Customer Immunohistochemistry-Frozen Mouse 05/09/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult spinal cord,Embryonic spinal cord
• Species: Mouse
• Lot: YZU00118051

reviewed by: Thomas Korff Simple Western Mouse 05/25/2018

Summary

• Application: Simple Western
• Sample Tested: Vein Lysate
• Species: Mouse
• Lot: YZU0118011

Comments

• Comments: This run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50). Lysis buffer: RIPA, prot. conc.: 0.2 µg/µl. Simple Western lane view shows one specific signal (~135 kDa) in lysates prepared from a mouse vein.

reviewed by: Shachar Dagan Immunohistochemistry-Frozen Mouse 04/02/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain
• Species: Mouse
• Lot: YZU0116121

Comments

• Comments: CD31/PECAM‑1 was detected in PFA fixed brain sections of adult mouse using Goat Anti-Mouse/PECAM‑1 (#AF3628) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Alexa568-conjugated Donkey anti-Goat
  IgG Secondary Antibody and counterstained with DAPI (blue).

reviewed by: Verified Customer Immunocytochemistry Mouse 05/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Liver Tissue
• Species: Mouse

Comments

• Comments: Frozen Tissue
  4% PFA fixation

reviewed by: Verified Customer Immunocytochemistry Mouse 05/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Liver Tissue
• Species: Mouse
• Lot: YZU0116121

Comments

• Comments: Frozen tissue
  Cold Acetone fixation

FAQs for CD31/PECAM-1 Antibody (AF3628). (Showing 1 - 1 of 1 FAQs).

1. Does CD31/PECAM-1 antibody (AF3628) work on Rat Paraffin embedded tissues(FFPE)?
   • For this antibody AF3628, it has been validated for mouse and rat and listed are multiple publications stating it was used for IHC-Paraffin.

Bioinformatics

• Gene Symbol: PECAM1
• Uniprot:
  • Q08481()