CD31/PECAM-1 Antibody [Unconjugated]

CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing guts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CD31/PECAM‑1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD31/PECAM-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
  Glu18-Lys590
  Accession # Q08481
• Specificity: Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
• Details of Functionality: ActivityAssaywCitation not found None None None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PECAM1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Unconjugated]

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM^-/- mice are deficient in chemokine-mediated chemotaxis (14).

1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
6. Ilan, N. et al. (2001) FASEB J. 15:362.
7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
10. Mamdouh, Z. et al. (2003) Nature 421:748.
11. Gao, C. et al. (2003) Blood 102:169.
12. Falati, S. et al. (2006) Blood 107:535.
13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD31/PECAM-1 Antibody (AF3628)(112)

Publications using AF3628

• B Dechaumet, D Cleret, MT Linossier, A Vanden-Bos, S Chanon, E Lefai, N Laroche, MH Lafage-Pro, L Vico Hypergravity as a gravitational therapy mitigates the effects of knee osteoarthritis on the musculoskeletal system in a murine model PLoS ONE, 2020;15(12):e0243098. 2020 [PMID: 33296408] (IHC, Mouse)
• K Greer, EKG Basso, C Kelly, A Cash, E Kowalski, S Cerna, CT Ocampo, X Wang, MH Theus Abrogation of atypical neurogenesis and vascular-derived EphA4 prevents repeated mild TBI-induced learning and memory impairments Scientific Reports, 2020;10(1):15374. 2020 [PMID: 32958852] (IHC, Mouse)
• A Julien, S Perrin, O Duchamp de, C Carvalho, M Bensidhoum, L Legeai-Mal, C Colnot FGFR3 in Periosteal Cells Drives Cartilage-to-Bone Transformation in Bone Repair Stem Cell Reports, 2020;0(0):. 2020 [PMID: 32916123] (IHC, Mouse)
• C Donato, L Kunz, F Castro-Gin, A Paasinen-S, K Strittmatt, BM Szczerba, R Scherrer, N Di Maggio, W Heusermann, O Biehlmaier, C Beisel, M Vetter, C Rochlitz, WP Weber, A Banfi, T Schroeder, N Aceto Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell Rep, 2020;32(10):108105. 2020 [PMID: 32905777] (IHC, Mouse)
• J Li, K Zhang, Q Zhang, X Zhou, L Wen, J Ma, L Niu, C Li PPAR-&amp;gamma Mediates Ta-VNS-Induced Angiogenesis and Subsequent Functional Recovery after Experimental Stroke in Rats Biomed Res Int, 2020;2020(0):8163789. 2020 [PMID: 32775443] (IHC, Rat)
• K Hosaka, Y Yang, T Seki, Q Du, X Jing, X He, J Wu, Y Zhang, H Morikawa, M Nakamura, M Scherzer, X Sun, Y Xu, T Cheng, X Li, X Liu, Q Li, Y Liu, A Hong, Y Chen, Y Cao Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors Nat Commun, 2020;11(1):3704. 2020 [PMID: 32709869] (IHC, Mouse)
• C Rocha-Rese, W Yang, W Li, D Kreisel, L Adamo, DL Mann Developmental changes in myocardial B cells mirror changes in B cells associated with different organs JCI Insight, 2020;5(16):. 2020 [PMID: 32663200] (IHC, Mouse)
• T Tamari, R Kawar-Jara, O Doppelt, B Giladi, N Sabbah, H Zigdon-Gil The Paracrine Role of Endothelial Cells in Bone Formation via CXCR4/SDF-1 Pathway Cells, 2020;9(6):. 2020 [PMID: 32466427] (IHC, Mouse)
• HM Shan, M Zang, Q Zhang, RB Shi, XJ Shi, M Mamtilahun, C Liu, LL Luo, X Tian, Z Zhang, GY Yang, Y Tang, J Pu, Y Wang Farnesoid X receptor knockout protects brain against ischemic injury through reducing neuronal apoptosis in mice J Neuroinflammation, 2020;17(1):164. 2020 [PMID: 32450881] (IHC, Mouse)
• L Kang, H Yu, X Yang, Y Zhu, X Bai, R Wang, Y Cao, H Xu, H Luo, L Lu, MJ Shi, Y Tian, W Fan, BQ Zhao Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after stroke Nat Commun, 2020;11(1):2488. 2020 [PMID: 32427863] (IHC, Mouse)
• ZL Grant, L Whitehead, VHY Wong, Z He, RY Yan, AR Miles, AV Benest, DO Bates, C Prahst, K Bentley, BV Bui, RC Symons, L Coultas Blocking endothelial apoptosis revascularises the retina in a model of ischemic retinopathy J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32427589] (IIHC, Mouse)
• H Iwamoto, H Suzuki, S Shimose, T Niizeki, M Nakano, T Shirono, S Okamura, Y Noda, N Kamachi, T Nakamura, A Masuda, T Sakaue, T Tanaka, D Nakano, M Sakai, T Yamaguchi, R Kuromatsu, H Koga, T Torimura Weekends-Off Lenvatinib for Unresectable Hepatocellular Carcinoma Improves Therapeutic Response and Tolerability toward Adverse Events Cancers (Basel), 2020;12(4):. 2020 [PMID: 32325921] (IHC, Mouse)
• D Zhu, TK Johnson, Y Wang, M Thomas, K Huynh, Q Yang, VC Bond, YE Chen, D Liu Macrophage M2 polarization induced by exosomes from adipose-derived stem cells contributes to the exosomal proangiogenic effect on mouse ischemic hindlimb Stem Cell Res Ther, 2020;11(1):162. 2020 [PMID: 32321589] (IHC-Fr, Mouse)
• X Lei, Y Zhong, L Huang, S Li, J Fu, L Zhang, Y Zhang, Q Deng, X Yu Identification of a novel tumor angiogenesis inhibitor targeting Shh/Gli1 signaling pathway in Non-small cell lung cancer Cell Death Dis, 2020;11(4):232. 2020 [PMID: 32286274] (IHC, Mouse)
• S Chen, C Xing, D Huang, C Zhou, B Ding, Z Guo, Z Peng, D Wang, X Zhu, S Liu, Z Cai, J Wu, J Zhao, Z Wu, Y Zhang, C Wei, Q Yan, H Wang, D Fan, L Liu, H Zhang, Y Cao Eradication of tumor growth by delivering novel photothermal selenium-coated tellurium nanoheterojunctions Sci Adv, 2020;6(15):eaay6825. 2020 [PMID: 32284997] (IHC, Mouse)
• T Kovács-Öll, E Ivanova, G Szarka, ÁJ Tengölics, B Völgyi, BT Sagdullaev Imatinib Sets Pericyte Mosaic in the Retina Int J Mol Sci, 2020;21(7):. 2020 [PMID: 32260484] (IHC-P, Mouse)
• MB Chen, AC Yang, H Yousef, D Lee, W Chen, N Schaum, B Lehallier, SR Quake, T Wyss-Coray Brain Endothelial Cells Are Exquisite Sensors of Age-Related Circulatory Cues Cell Rep, 2020;30(13):4418-4432.e4. 2020 [PMID: 32234477] (IHC, Mouse)
• W Tang, J Guo, R Gu, B Lei, X Ding, J Ma, G Xu MicroRNA-29b-3p inhibits cell proliferation and angiogenesis by targeting VEGFA and PDGFB in retinal microvascular endothelial cells Mol. Vis., 2020;26(0):64-75. 2020 [PMID: 32165827] (ICC, Rat)
• Z Li, EA Korhonen, A Merlini, J Strauss, E Wihuri, H Nurmi, S Antila, J Paech, U Deutsch, B Engelhardt, S Chintharla, GY Koh, A Flügel, K Alitalo Angiopoietin-2 blockade ameliorates autoimmune neuroinflammation by inhibiting leukocyte recruitment into the CNS J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32149735] (IHC-P, Mouse)
• A Najdahmadi, AM Smink, P de Vos, JRT Lakey, E Botvinick Non-Invasive Monitoring of Oxygen Tension and Oxygen Transport Inside Subcutaneous Devices After H2S Treatment Cell Transplant, 2020;29(0):9636897198939. 2020 [PMID: 32024377] (IHC, Mouse)
• H Yamamoto, T Kon, Y Omori, T Furukawa Functional and Evolutionary Diversification of Otx2 and Crx in Vertebrate Retinal Photoreceptor and Bipolar Cell Development Cell Rep, 2020;30(3):658-671.e5. 2020 [PMID: 31968244] (IF, Mouse)
• S Colijn, V Muthukumar, J Xie, S Gao, CT Griffin Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis Dis Model Mech, 2020;13(1):. 2020 [PMID: 31953345] (IHC, Mouse)
• JH Wu, YN Li, AQ Chen, CD Hong, CL Zhang, HL Wang, YF Zhou, PC Li, Y Wang, L Mao, YP Xia, QW He, HJ Jin, ZY Yue, B Hu Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy EMBO Mol Med, 2020;12(2):e10154. 2020 [PMID: 31943789] (IHC, Mouse)
• J Vigne, S Bay, R Aid-Launai, G Pariscoat, G Rucher, J Sénémaud, A Truffier, N Anizan, G Even, C Ganneau, F Andreata, M Le Borgne, A Nicoletti, D Le Guludec, G Caligiuri, F Rouzet Cleaved CD31 as a target for in vivo molecular imaging of inflammation Sci Rep, 2019;9(1):19560. 2019 [PMID: 31863037] (IHC-P, Rat)
• N Lalaoui, SE Boyden, H Oda, GM Wood, DL Stone, D Chau, L Liu, M Stoffels, T Kratina, KE Lawlor, KJM Zaal, PM Hoffmann, N Etemadi, K Shield-Art, C Biben, WL Tsai, MD Blake, HS Kuehn, D Yang, H Anderton, N Silke, L Wachsmuth, L Zheng, NS Moura, DB Beck, G Gutierrez-, AK Ombrello, GP Pinto-Pata, AJ Kueh, MJ Herold, C Hall, H Wang, JJ Chae, NI Dmitrieva, M McKenzie, A Light, BK Barham, A Jones, TM Romeo, Q Zhou, I Aksentijev, JC Mullikin, AJ Gross, AK Shum, ED Hawkins, SL Masters, MJ Lenardo, M Boehm, SD Rosenzweig, M Pasparakis, AK Voss, M Gadina, DL Kastner, J Silke Mutations that prevent caspase cleavage of RIPK1 cause autoinflammatory disease Nature, 2020;577(7788):103-108. 2020 [PMID: 31827281] (IHC, Human)
• G Luxán, J Stewen, N Díaz, K Kato, SK Maney, A Aravamudha, F Berkenfeld, N Nagelmann, HC Drexler, D Zeuschner, C Faber, H Schillers, S Hermann, J Wiseman, JM Vaquerizas, ME Pitulescu, RH Adams Endothelial EphB4 maintains vascular integrity and transport function in adult heart Elife, 2019;8(0):. 2019 [PMID: 31782728] (IHC, Mouse)
• SA Nam, E Seo, JW Kim, HW Kim, HL Kim, K Kim, TM Kim, JH Ju, IG Gomez, K Uchimura, BD Humphreys, CW Yang, JY Lee, J Kim, DW Cho, BS Freedman, YK Kim Graft immaturity and safety concerns in transplanted human kidney organoids Exp. Mol. Med., 2019;51(11):145. 2019 [PMID: 31776328] (IHC, Human)
• N Sabbah, T Tamari, R Elimelech, O Doppelt, U Rudich, H Zigdon-Gil Predicting Angiogenesis by Endothelial Progenitor Cells Relying on In-Vitro Function Assays and VEGFR-2 Expression Levels Biomolecules, 2019;9(11):. 2019 [PMID: 31717420] (IHC, Mouse)
• CR Park, JH Jo, MG Song, JY Park, YH Kim, H Youn, SH Paek, JK Chung, JM Jeong, YS Lee, KW Kang Secreted protein acidic and rich in cysteine mediates active targeting of human serum albumin in U87MG xenograft mouse models Theranostics, 2019;9(24):7447-7457. 2019 [PMID: 31695779] (IHC, Mouse)
• W Song, A Chiu, LH Wang, RE Schwartz, B Li, N Bouklas, DT Bowers, D An, SH Cheong, JA Flanders, Y Pardo, Q Liu, X Wang, VK Lee, G Dai, M Ma Engineering transferrable microvascular meshes for subcutaneous islet transplantation Nat Commun, 2019;10(1):4602. 2019 [PMID: 31601796] (IHC, Mouse)
• AA Lombardi, AA Gibb, E Arif, DW Kolmetzky, D Tomar, TS Luongo, P Jadiya, EK Murray, PK Lorkiewicz, G Hajnóczky, E Murphy, ZP Arany, DP Kelly, KB Margulies, BG Hill, JW Elrod Mitochondrial calcium exchange links metabolism with the epigenome to control cellular differentiation Nat Commun, 2019;10(1):4509. 2019 [PMID: 31586055] (IHC-P, Mouse)
• D Asano, M Hokazono, S Hirano, A Morita, T Nakahara Cellular Mechanisms of Angiogenesis in Neonatal Rat Models of Retinal Neurodegeneration Int J Mol Sci, 2019;20(19):. 2019 [PMID: 31557901] (Cell Culture, Rat)
• DK Malipatlol, P Patel, F Sjöberg, S Devarakond, M Kalm, E Angenete, EB Lindskog, R Grandér, L Persson, A Stringer, U Wilderäng, J Swanpalmer, HG Kuhn, G Steineck, C Bull Long-term mucosal injury and repair in a murine model of pelvic radiotherapy Sci Rep, 2019;9(1):13803. 2019 [PMID: 31551503] (IHC-P, Mouse)
• S Ni, Z Luo, L Jiang, Z Guo, P Li, X Xu, Y Cao, C Duan, T Wu, C Li, H Lu, J Hu UTX/KDM6A Deletion Promotes Recovery of Spinal Cord Injury by Epigenetically Regulating Vascular Regeneration Mol. Ther., 2019;0(0):. 2019 [PMID: 31495776] (IHC, Mouse)
• Y Ma, KN Su, D Pfau, VS Rao, X Wu, X Hu, L Leng, X Du, M Piecychna, K Bedi, SG Campbell, A Eichmann, JM Testani, KB Margulies, R Bucala, LH Young Cardiomyocyte d-dopachrome tautomerase protects against heart failure JCI Insight, 2019;4(17):. 2019 [PMID: 31484822] (IHC-P, Mouse)
• T Wyseure, T Yang, JY Zhou, EJ Cooke, B Wanko, M Olmer, R Agashe, Y Morodomi, N Behrendt, M Lotz, J Morser, A von Drygal, LO Mosnier TAFI deficiency causes maladaptive vascular remodeling after hemophilic joint bleeding JCI Insight, 2019;0(0):. 2019 [PMID: 31465300] (IHC-P, Human)
• P Jirkof, M Durst, R Klopfleisc, R Palme, C Thöne-Rein, F Buttgereit, K Schmidt-Bl, A Lang Administration of Tramadol or Buprenorphine via the drinking water for post-operative analgesia in a mouse-osteotomy model Sci Rep, 2019;9(1):10749. 2019 [PMID: 31341225] (IHC, Mouse)
• BW Dulken, MT Buckley, P Navarro Ne, N Saligrama, R Cayrol, DS Leeman, BM George, SC Boutet, K Hebestreit, JV Pluvinage, T Wyss-Coray, IL Weissman, H Vogel, MM Davis, A Brunet Single-cell analysis reveals T cell infiltration in old neurogenic niches Nature, 2019;0(0):. 2019 [PMID: 31270459] (IHC, Mouse)
• R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes Nat Commun, 2019;10(1):2817. 2019 [PMID: 31249304] (IHC, Mouse)
• H Luo, L Wei, L Lu, L Kang, Y Cao, X Yang, X Bai, W Fan, BQ Zhao Transfusion of Resting Platelets Reduces Brain Hemorrhage After Intracerebral Hemorrhage and tPA-Induced Hemorrhage After Cerebral Ischemia Front Neurosci, 2019;13(0):338. 2019 [PMID: 31024246] (IHC, Mouse)
• SG Romeo, KM Alawi, J Rodrigues, A Singh, AP Kusumbe, SK Ramasamy Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation Nat. Cell Biol., 2019;21(4):430-441. 2019 [PMID: 30936475] (IHC-Fr, Mouse)
• AR Bhagwani, S Hultman, D Farkas, R Moncayo, K Dandamudi, AK Zadu, CD Cool, L Farkas Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension PLoS ONE, 2019;14(3):e0213890. 2019 [PMID: 30883593] (Cell Selection, Rat)
• T Wohlfahrt, S Rauber, S Uebe, M Luber, A Soare, A Ekici, S Weber, AE Matei, CW Chen, C Maier, E Karouzakis, HP Kiener, E Pachera, C Dees, C Beyer, C Daniel, K Gelse, AE Kremer, E Naschberge, M Stürzl, F Butter, M Sticherlin, S Finotto, A Kreuter, MH Kaplan, A Jüngel, S Gay, SL Nutt, DW Boykin, GMK Poon, O Distler, G Schett, JHW Distler, A Ramming PU.1 controls fibroblast polarization and tissue fibrosis Nature, 2019;0(0):. 2019 [PMID: 30700907] (IHC, Mouse)
• KJ Ray, MA Simard, JR Larkin, J Coates, P Kinchesh, SC Smart, GS Higgins, M Chappell, N Sibson Tumour pH and protein concentration contribute to the signal of amide proton transfer magnetic resonance imaging Cancer Res., 2019;0(0):. 2019 [PMID: 30679178] (IHC, Rat)
• MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA Cell Rep, 2018;25(13):3811-3827.e7. 2018 [PMID: 30590051] (Ifs, Mouse)
• JH Wu, YF Zhou, CD Hong, AQ Chen, Y Luo, L Mao, YP Xia, QW He, HJ Jin, M Huang, YN Li, B Hu Semaphorin-3A protects against neointimal hyperplasia after vascular injury EBioMedicine, 2019;39(0):95-108. 2019 [PMID: 30579864] (ICC, Mouse)
• H Chagraoui, MS Kristianse, JP Ruiz, A Serra-Barr, J Richter, E Hall-Ponse, N Gray, D Waithe, K Clark, P Hublitz, E Repapi, G Otto, P Sopp, S Taylor, S Thongjuea, P Vyas, C Porcher SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells Nat Commun, 2018;9(1):5375. 2018 [PMID: 30560907] (ICC, Mouse)
• SH Choi, AR Kim, JK Nam, JM Kim, JY Kim, HR Seo, HJ Lee, J Cho, YJ Lee Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization Nat Commun, 2018;9(1):5108. 2018 [PMID: 30504836] (IHC, Mouse)
• MJ Carr, JS Toma, APW Johnston, PE Steadman, SA Yuzwa, N Mahmud, PW Frankland, DR Kaplan, FD Miller Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30503141] (IHC, Mouse)
• MA Mäe, T Li, G Bertuzzi, E Raschperge, M Vanlandewi, L He, K Nahar, A Dalheim, JJ Hofmann, B Laviña, A Keller, C Betsholtz, G Genové Prolonged systemic hyperglycemia does not cause pericyte loss and permeability at the mouse blood-brain barrier Sci Rep, 2018;8(1):17462. 2018 [PMID: 30498224] (IHC-P, Mouse)
• K Hübner, P Cabochette, R Diéguez-Hu, C Wiesner, Y Wakayama, KS Grassme, M Hubert, S Guenther, HG Belting, M Affolter, RH Adams, B Vanhollebe, W Herzog Wnt/?-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling Nat Commun, 2018;9(1):4860. 2018 [PMID: 30451830] (ICC, Mouse)
• S Aratani, M Tagawa, S Nagasaka, Y Sakai, A Shimizu, S Tsuruoka Radiation-induced premature cellular senescence involved in glomerular diseases in rats Sci Rep, 2018;8(1):16812. 2018 [PMID: 30429495] (IHC-P, Rat)
• AC Title, SJ Hong, ND Pires, L Hasenöhrl, S Godbersen, N Stokar-Reg, DP Bartel, M Stoffel Genetic dissection of the miR-200-Zeb1 axis reveals its importance in tumor differentiation and invasion Nat Commun, 2018;9(1):4671. 2018 [PMID: 30405106] (IHC-P, Mouse)
• PLP Smith, A Mottahedin, P Svedin, CJ Mohn, H Hagberg, J Ek, C Mallard Peripheral myeloid cells contribute to brain injury in male neonatal mice J Neuroinflammation, 2018;15(1):301. 2018 [PMID: 30376851]
• OA Stone, M El-Brolosy, K Wilhelm, X Liu, AM Romão, E Grillo, JKH Lai, S Günther, S Jeratsch, C Kuenne, IC Lee, T Braun, MM Santoro, JW Locasale, M Potente, DYR Stainier Loss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cells Nat Commun, 2018;9(1):4077. 2018 [PMID: 30301887] (IHC, Mouse)
• U Yrlid, M Holm, M Levin, S Alsén, M Lindbom, L Glise, N Bergh, J Borén, P Fogelstran Endothelial repair is dependent on CD11c+ leukocytes to establish regrowing endothelial sheets with high cellular density J. Leukoc. Biol., 2018;0(0):. 2018 [PMID: 30265749] (IHC, Mouse)
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Reviews for CD31/PECAM-1 Antibody (AF3628) (6)

Average Rating: 5

(Based on 6 reviews)

We have 6 reviews tested in 2 species: Mouse, Mouse brain tissue and Mouse.

reviewed by: Bhavin Shah Mouse brain tissue and Mouse 05/10/2019

Summary

• Sample Tested: P12 brain cortex,P21 brain cortex
• Species: Mouse brain tissue and Mouse
• Lot: YZU0117011

Comments

• Comments: Blocking with 10%donkey serum and PBST &#40;.3%TritonX100&#41;

reviewed by: Ricardo Vieira IHC-Fr Mouse 05/09/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult spinal cord,Embryonic spinal cord
• Species: Mouse
• Lot: YZU00118051

reviewed by: Anonymous Simple Western Mouse 05/25/2018

Summary

• Application: Simple Western
• Sample Tested: Vein Lysate
• Species: Mouse
• Lot: YZU0118011

Comments

• Comments: This run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50). Lysis buffer: RIPA, prot. conc.: 0.2 µg/µl. Simple Western lane view shows one specific signal (~135 kDa) in lysates prepared from a mouse vein.

reviewed by: Anonymous IHC-Fr Mouse 04/02/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain
• Species: Mouse
• Lot: YZU0116121

Comments

• Comments: CD31/PECAM‑1 was detected in PFA fixed brain sections of adult mouse using Goat Anti-Mouse/PECAM‑1 (#AF3628) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Alexa568-conjugated Donkey anti-Goat
  IgG Secondary Antibody and counterstained with DAPI (blue).

reviewed by: Jan Martinek ICC Mouse 05/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Liver Tissue
• Species: Mouse

Comments

• Comments: Frozen Tissue
  4% PFA fixation

reviewed by: Jan Martinek ICC Mouse 05/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Liver Tissue
• Species: Mouse
• Lot: YZU0116121

Comments

• Comments: Frozen tissue
  Cold Acetone fixation

FAQs for CD31/PECAM-1 Antibody (AF3628). (Showing 1 - 1 of 1 FAQs).

1. Does CD31/PECAM-1 antibody (AF3628) work on Rat Paraffin embedded tissues(FFPE)?
   • For this antibody AF3628, it has been validated for mouse and rat and listed are multiple publications stating it was used for IHC-Paraffin.

Bioinformatics

• Gene Symbol: PECAM1
• Uniprot:
  • Q08481()