TLR9 Antibody - BSA Free

Images

 
Biological Strategies: Western Blot: TLR9 Antibody [NBP1-76680] - (G) Levels of TLR9 (NBP1-76680), COX4 (NB110-39115), MyD88, and NF-kB protein by Western blot. (H) Relative expression of TLR9 and COX4 protein. ...read more
Immunocytochemistry/ Immunofluorescence: TLR9 Antibody [NBP1-76680] - Jurkat cells with TLR9antibody at 2 ug/ml.
Western Blot: TLR9 Antibody [NBP1-76680] - Analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide
Orthogonal Strategies: Western Blot: TLR9 Antibody [NBP1-76680] - (A) Levels of COX4 (NB110-39115), TLR9 (NBP1-76680), MyD88, and NF-kB mRNA. (B) Levels of COX4 (NB110-39115), TLR9 (NBP1-76680), MyD88, and NF-kB ...read more
Immunocytochemistry/ Immunofluorescence: TLR9 Antibody [NBP1-76680] - Mouse Spleen tissue.
Inhibition of endogenous miR-27a/b function increases human PINK1 expression. a Schematic diagram of sequence alignment of miR-27a/b and anti-miR-27a/b. Asterisks (*) indicate conserved nucleotides. b Inhibition of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

TLR9 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9. The immunogen is located within amino acids 840 - 890 of TLR9. Amino Acid Squence: RGRQSGRDEDALPYD
Specificity
At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR9
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2-20 ug/mL
  • Western Blot 1 ug/ml
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Agonist
Control Peptide
TLR9 Peptide (NBP1-76680PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-76680 in the following applications:

Publications
Read Publications using
NBP1-76680 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for TLR9 Antibody - BSA Free

  • CD289 antigen
  • CD289
  • TLR9
  • toll-like receptor 9

Background

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR9 Antibody (NBP1-76680)(4)

Review for TLR9 Antibody (NBP1-76680) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-76680:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot TLR9 NBP1-76680
Enlarge
4
reviewed by:
Prashant Rai
WB Mouse 07/17/2019
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Summary

ApplicationWestern Blot
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
Lot3739-1604

Comments

CommentsSamples ran on Bis-Tris gradient gel.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR9 Antibody (NBP1-76680) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TLR9 Products

Research Areas for TLR9 Antibody (NBP1-76680)

Find related products by research area.

Blogs on TLR9.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR9: Tollgate to Immunity
Toll-like receptors (TLRs) play an essential role in the activation of innate immunity, and TLRs are expressed in a large number of immune cells as well as in epithelial cells. TLR9 recognizes synthetic oligodeoxynucleotides (ODN) containing unmethyla...  Read full blog post.

TLR9, Infectious Disease and Cancer
Toll-like receptor 9 (TLR9) is a protein encoded by TLR9 gene in humans. It is also known as cluster of differentiation 289 (CD289) and is a member of TLR family. Proteins from TLR family are transmembrane proteins that expressed in both antigen-resen...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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Recent Reviews

4
5
0
4
1
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0
2
0
1
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Prashant Rai
07/17/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TLR9
Uniprot