TLR9 Antibody - BSA Free

Western Blot: TLR9 Antibody [NBP1-76680] - (G) Levels of TLR9 (NBP1-76680), COX4 (NB110-39115), MyD88, and NF-kB protein by Western blot. (H) Relative expression of TLR9 and COX4 protein. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: TLR9 Antibody [NBP1-76680] - Jurkat cells with TLR9antibody at 2 ug/ml.

Western Blot: TLR9 Antibody [NBP1-76680] - Analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide

Western Blot: TLR9 Antibody [NBP1-76680] - (A) Levels of COX4 (NB110-39115), TLR9 (NBP1-76680), MyD88, and NF-kB mRNA. (B) Levels of COX4 (NB110-39115), TLR9 (NBP1-76680), MyD88, and NF-kB protein by Western blot. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: TLR9 Antibody [NBP1-76680] - Mouse Spleen tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TLR9 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9. The immunogen is located within amino acids 840 - 890 of TLR9. Amino Acid Squence: RGRQSGRDEDALPYD
• Specificity: At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TLR9
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Agonist:
  • CpG oligodeoxynucleotides with negative control, TLR9 ligand NBP2-26232
• Control Peptide:

  TLR9 Peptide (NBP1-76680PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TLR9 Antibody - BSA Free

• CD289 antigen
• CD289
• TLR9
• toll-like receptor 9

Background

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR9 Antibody (NBP1-76680)(3)

Publications using NBP1-76680

• Lin JY, Jing R, Lin F et al. High Tidal Volume Induces Mitochondria Damage and Releases Mitochondrial DNA to Aggravate the Ventilator-Induced Lung Injury. Front Immunol. 2018-07-03 [PMID: 30018615] (WB, Rat)
• Li W, Zhu S, Li J et al. Serum Amyloid A Stimulates PKR Expression and HMGB1 Release Possibly through TLR4/RAGE Receptors. Mol Med 2015-01-01 [PMID: 26052716]
• Xu L, Wen Z, Zhou Y et al. MicroRNA-7-regulated TLR9 signaling-enhanced growth and metastatic potential of human lung cancer cells by altering the phosphoinositide-3-kinase, regulatory subunit 3/Akt pathway Mol Biol Cell 2013-01-01 [PMID: 23135998] (Human)

Bioinformatics

• Gene Symbol: TLR9
• Uniprot:
  • AAH32713(Human)