B7-2/CD86 Antibody (BU63) Summary
ARH-77 (B-lymphoblastoid cell line)
DC Maturation Marker
Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 0.5-1ug/ml
- Flow Cytometry 0.5-1ug/million cells
- Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
- Immunohistochemistry 2 - 4 ug/ml
- Immunohistochemistry-Paraffin 2 - 4ug/ml
- Immunoprecipitation 0.5-1ug/500ug protein lysate
- Knockout Validated
Human Chromosome Location: 3q13.33
Functional Studies (Order Ab without Azide), Immunohistology (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C.
10mM PBS and 0.05% BSA
0.05% Sodium Azide
Protein A or G purified
Alternate Names for B7-2/CD86 Antibody (BU63)
- Activation B7-2 antigen
- B7-2 antigen
- B-lymphocyte activation antigen B7-2
- CD28 antigen ligand 2
- CD28LG2B7-2 antigen)
- CD86 antigen
- CD86 molecule
- CTLA-4 counter-receptor B7.2
- T-lymphocyte activation antigen CD86
CD86, also called B7-2, belongs to the immunoglobulin superfamily of proteins critical in driving innate and adaptive immune responses (1). CD86, along with CD80 present on antigen presenting cells such as dendritic cells (DCs) are costimulatory molecules in the immune synapse between such as DC-T cells. CD86 and CD80 serve as receptors for CD28, resulting in activation, while binding CTLA-4 may downregulate activation (2). CD86 engagement with CD28 presentation on T cells results in DC activation and triggering of key pro-inflammatory cytokine responses through NF-kB. Studies suggest differentiable roles of CD86 and CD80 in adaptive inflammatory responses through in vivo studies such as in graft rejection (3). Additional studies also indicate different roles of both CD86 and CD80 in innate immune responses such as in sepsis (4). In the latter study it appears that sepsis and initial septic shock, a pro-inflammatory cytokine storm notably comprised of IL-6, TNFa, and IL-1b, is associated with CD80 regulation, while CD86 contribution at least in this model was not as prominent.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Additional B7-2/CD86 Products
Bioinformatics Tool for B7-2/CD86 Antibody (NBP2-25208)
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Blogs on B7-2/CD86.
CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated... Read full blog post.