B7-2/CD86 Antibody (BU63)


Genetic Strategies: Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF ...read more
Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Macrophage polarization states. Distribution and proportion of CD86+ macrophages (brown) were identified using IHC procedures. Intensity for the color ...read more
Immunocytochemistry/ Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are ...read more
Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Effects of 20(S)-Rg3 on macrophage polarization in atherosclerotic lesions of diabetic ApoE-/- mice. Co-immunofluorescence staining for macrophage ...read more
Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of PFA-fixed Ramos cells. B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong ...read more
Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of human PBMCs using B7-2/CD86 Antibody (BU63); Goat anti-Mouse IgG-CF488 (red); Isotype Control (green).

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, KO
0.2 mg/ml
Validated by:

Genetic Strategies


Order Details

B7-2/CD86 Antibody (BU63) Summary

ARH-77 (B-lymphoblastoid cell line)
Cell Surface
DC Maturation Marker
Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
IgG1 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 1-2ug/million cells
  • Functional
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2-4ug/ml
  • Knockout Validated
Application Notes
Functional Studies: Order Ab without Azide.
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
IHC-fr reported in the literature (PMID: 29867472, 31629891)
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP2-25208 in the following applications:

Packaging, Storage & Formulations

Store at 4C.
10 mM PBS with 0.05% BSA
0.05% Sodium Azide
0.2 mg/ml
Protein A or G purified


200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml (NBP2-34569).

Alternate Names for B7-2/CD86 Antibody (BU63)

  • Activation B7-2 antigen
  • B70
  • B7-2 antigen
  • B72
  • B7-2
  • B-lymphocyte activation antigen B7-2
  • BU63
  • CD28 antigen ligand 2
  • CD28LG2B7-2 antigen)
  • CD86 antigen
  • CD86 molecule
  • CD86
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • LAB72
  • MGC34413
  • T-lymphocyte activation antigen CD86


CD86, also called B7-2, belongs to the immunoglobulin superfamily of proteins critical in driving innate and adaptive immune responses (1). CD86, along with CD80 present on antigen presenting cells such as dendritic cells (DCs) are costimulatory molecules in the immune synapse between such as DC-T cells. CD86 and CD80 serve as receptors for CD28, resulting in activation, while binding CTLA-4 may downregulate activation (2). CD86 engagement with CD28 presentation on T cells results in DC activation and triggering of key pro-inflammatory cytokine responses through NF-kB. Studies suggest differentiable roles of CD86 and CD80 in adaptive inflammatory responses through in vivo studies such as in graft rejection (3). Additional studies also indicate different roles of both CD86 and CD80 in innate immune responses such as in sepsis (4). In the latter study it appears that sepsis and initial septic shock, a pro-inflammatory cytokine storm notably comprised of IL-6, TNFa, and IL-1b, is associated with CD80 regulation, while CD86 contribution at least in this model was not as prominent.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-2/CD86 Antibody (NBP2-25208)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IF, IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP2-25208 Applications Species
Luo ZW, Xia K, Liu YW et al. Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8+ T Cells and Macrophages International journal of nanomedicine Apr 20 2021 [PMID: 33907401] (IHC, Human) IHC Human
Liu P, Peng C, Chen X et al. Acitretin Promotes the Differentiation of Myeloid-Derived Suppressor Cells in the Treatment of Psoriasis Frontiers in Medicine Mar 23 2021 [PMID: 33834031] (IHC-P, Mouse) IHC-P Mouse
Zhu LF, Li L, Wang XQ et al. M1 macrophages regulate TLR4/AP1 via paracrine to promote alveolar bone destruction in periodontitis Oral Dis Jul 30 2019 [PMID: 31361069]
Liu D, Guo M, Zhou P et al. TSLP promote M2 macrophages polarization and cardiac healing after myocardial infarction Biochem. Biophys. Res. Commun. 2019 Jun 18 [PMID: 31227217] (IF, Mouse) IF Mouse
Kaushik NK, Kaushik N, Adhikari M et al. Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages Cancers (Basel) Jun 18 2019 [PMID: 31216715] (IHC-P, Mouse) IHC-P Mouse
Yang D, Shen L, Chen R et al. The Effect of Talaromyces Marneffei Infection on CD86 Expression in THP-1 Cells Research Square Dec 15 2020
Klinge U, Dievernich A, Tolba R et al. CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining J. Biomed. Mater. Res. Part B Appl. Biomater. May 30 2020 [PMID: 32475069] (ICC/IF, Human) ICC/IF Human
Li Ml, Su Xm, Ren Y et Al. HDAC8 inhibitor attenuates airway responses to antigen stimulus through synchronously suppressing galectin-3 expression and reducing macrophage-2 polarization Respir. Res. Feb 28 2020 [PMID: 32111211] (IHC, Mouse) IHC Mouse
Shehata MM, Saad Eldien HM, Meligy FY, Bahaidarh SY The Possible Protective Role of Barley Seeds on the Spleen after Administration of Glucocorticoids in Adult Albino Rats: A Histological and Immunohistochemical Study J Microsc Ultrastruct Nov 18 2019 [PMID: 31803571] (Rat) Rat
Joly-Amado A, Davtyan H, Serraneau K et al. Active immunization with tau epitope in a mouse model of tauopathy induced strong antibody response together with improvement in short memory and pSer396-tau pathology. Neurobiol Dis. 2020 Feb [PMID: 31629891] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 14 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for B7-2/CD86 Antibody (NBP2-25208) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34569AF350
Alexa Fluor 405 NBP2-34569AF405
Alexa Fluor 488 NBP2-34569AF488
Alexa Fluor 532 NBP2-34569AF532
Alexa Fluor 594 NBP2-34569AF594
Alexa Fluor 647 NBP2-34569AF647
Alexa Fluor 700 NBP2-34569AF700
Alexa Fluor 750 NBP2-34569AF750
Allophycocyanin NBP2-34569APC
Allophycocyanin/Cy7 NBP2-34569APCCY7
Biotin NBP2-34569B
DyLight 350 NBP2-34569UV
DyLight 405 NBP2-34569V
DyLight 488 NBP2-34569G
DyLight 550 NBP2-34569R
DyLight 594 NBP2-34569DL594
DyLight 650 NBP2-34569C
DyLight 680 NBP2-34569FR
DyLight 755 NBP2-34569IR
FITC NBP2-34569F
HRP NBP2-34569H
Janelia Fluor 549 NBP2-34569JF549
Janelia Fluor 646 NBP2-34569JF646
PE NBP2-34569PE
PE/Atto594 NBP2-34569PEATT594
PE/Cy5.5 NBP2-34569PECY55
PE/Cy7 NBP2-34569PECY7
PerCP NBP2-34569PCP

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Blogs on B7-2/CD86.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Gene Symbol CD86