B7-2/CD86 Antibody (BU63)

Images

 
Genetic Strategies: Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF ...read more
Immunocytochemistry/ Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG conjugated to CF488 ...read more
Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong ...read more
Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of human PBMCs using B7-2/CD86 Antibody (BU63); Goat anti-Mouse IgG-CF488 (red); Isotype Control (green).
Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of PFA-fixed Ramos cells. B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, KO
Clone
BU63
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

B7-2/CD86 Antibody (BU63) Summary

Immunogen
ARH-77 (B-lymphoblastoid cell line)
Localization
Cell Surface
Marker
DC Maturation Marker
Specificity
Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD86
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • Knockout Validated
Application Notes
Human Chromosome Location: 3q13.33
Functional Studies (Order Ab without Azide), Immunohistology (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-25208 in the following applications:

  • 1 publication
  • IF
    1 publication
  • IHC
    1 publication
  • 1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for B7-2/CD86 Antibody (BU63)

  • Activation B7-2 antigen
  • B70
  • B7-2 antigen
  • B72
  • B7-2
  • B-lymphocyte activation antigen B7-2
  • BU63
  • CD28 antigen ligand 2
  • CD28LG2B7-2 antigen)
  • CD86 antigen
  • CD86 molecule
  • CD86
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • LAB72
  • MGC34413
  • T-lymphocyte activation antigen CD86

Background

CD86, also called B7-2, belongs to the immunoglobulin superfamily of proteins critical in driving innate and adaptive immune responses (1). CD86, along with CD80 present on antigen presenting cells such as dendritic cells (DCs) are costimulatory molecules in the immune synapse between such as DC-T cells. CD86 and CD80 serve as receptors for CD28, resulting in activation, while binding CTLA-4 may downregulate activation (2). CD86 engagement with CD28 presentation on T cells results in DC activation and triggering of key pro-inflammatory cytokine responses through NF-kB. Studies suggest differentiable roles of CD86 and CD80 in adaptive inflammatory responses through in vivo studies such as in graft rejection (3). Additional studies also indicate different roles of both CD86 and CD80 in innate immune responses such as in sepsis (4). In the latter study it appears that sepsis and initial septic shock, a pro-inflammatory cytokine storm notably comprised of IL-6, TNFa, and IL-1b, is associated with CD80 regulation, while CD86 contribution at least in this model was not as prominent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-2/CD86 Antibody (NBP2-25208)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IF
(1)
IHC
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP2-25208 Applications Species
Zhu LF, Li L, Wang XQ et al. M1 macrophages regulate TLR4/AP1 via paracrine to promote alveolar bone destruction in periodontitis Oral Dis Jul 30 2019 [PMID: 31361069]
Liu D, Guo M, Zhou P et al. TSLP promote M2 macrophages polarization and cardiac healing after myocardial infarction Biochem. Biophys. Res. Commun. 2019 Jun 18 [PMID: 31227217] (IF, Mouse) IF Mouse
Kaushik NK, Kaushik N, Adhikari M et al. Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages Cancers (Basel) Jun 18 2019 [PMID: 31216715] (IHC-P, Mouse) IHC-P Mouse
Zhao J, Zhang Z, Xue Y et al. Anti-tumor macrophages activated by ferumoxytol combined or surface-functionalized with the TLR3 agonist poly (I : C) promote melanoma regression Theranostics Nov 29 2018 [PMID: 30613299] (ICC/IF, Human) ICC/IF Human
Rivera Del Alamo M, Diaz-Lobo M, Busquets S et al. Specific expression pattern of tissue cytokines analyzed through the Surface Acoustic Wave technique is associated with age-related spontaneous benign prostatic hyperplasia in rats. Biochem Biophys Rep 2018 [PMID: 29872731] (WB, Rat) WB Rat
Guo M, Xiao J, Sheng X et al. Ginsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype Front Pharmacol May 9 2018 [PMID: 29867472] (IHC, Mouse) IHC Mouse
Schliefsteiner C, Peinhaupt M, Kopp S et al. Human Placental Hofbauer Cells Maintain an Anti-inflammatory M2 Phenotype despite the Presence of Gestational Diabetes Mellitus Front Immunol 2017 Aug 21 [PMID: 28824621] (WB, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for B7-2/CD86 Antibody (NBP2-25208) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34569AF350
Alexa Fluor 405 NBP2-34569AF405
Alexa Fluor 488 NBP2-34569AF488
Alexa Fluor 532 NBP2-34569AF532
Alexa Fluor 594 NBP2-34569AF594
Alexa Fluor 647 NBP2-34569AF647
Alexa Fluor 700 NBP2-34569AF700
Alexa Fluor 750 NBP2-34569AF750
Allophycocyanin NBP2-34569APC
Allophycocyanin/Cy7 NBP2-34569APCCY7
Biotin NBP2-34569B
DyLight 350 NBP2-34569UV
DyLight 405 NBP2-34569V
DyLight 488 NBP2-34569G
DyLight 550 NBP2-34569R
DyLight 594 NBP2-34569DL594
DyLight 650 NBP2-34569C
DyLight 680 NBP2-34569FR
DyLight 755 NBP2-34569IR
FITC NBP2-34569F
HRP NBP2-34569H
Janelia Fluor 549 NBP2-34569JF549
Janelia Fluor 646 NBP2-34569JF646
PE NBP2-34569PE
PE/Atto594 NBP2-34569PEATT594
PE/Cy5.5 NBP2-34569PECY55
PE/Cy7 NBP2-34569PECY7
PerCP NBP2-34569PCP

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Bioinformatics Tool for B7-2/CD86 Antibody (NBP2-25208)

Discover related pathways, diseases and genes to B7-2/CD86 Antibody (NBP2-25208). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on B7-2/CD86.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Bioinformatics

Gene Symbol CD86
Uniprot