B7-2/CD86 Antibody (BU63)

Images

 
Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong ...read more
Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Surface staining of CD86 on 10^6 PBMC using 0.25 ug of CD86 antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
BU63
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

B7-2/CD86 Antibody (BU63) Summary

Immunogen
ARH-77 (B-lymphoblastoid cell line)
Localization
Cell Surface
Marker
DC Maturation Marker
Specificity
Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD86
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
Application Notes
Human Chromosome Location: 3q13.33
Functional Studies (Order Ab without Azide), Immunohistology (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for B7-2/CD86 Antibody (BU63)

  • Activation B7-2 antigen
  • B70
  • B7-2 antigen
  • B72
  • B7-2
  • B-lymphocyte activation antigen B7-2
  • BU63
  • CD28 antigen ligand 2
  • CD28LG2B7-2 antigen)
  • CD86 antigen
  • CD86 molecule
  • CD86
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • LAB72
  • MGC34413
  • T-lymphocyte activation antigen CD86

Background

CD86, also called B7-2, belongs to the immunoglobulin superfamily of proteins critical in driving innate and adaptive immune responses (1). CD86, along with CD80 present on antigen presenting cells such as dendritic cells (DCs) are costimulatory molecules in the immune synapse between such as DC-T cells. CD86 and CD80 serve as receptors for CD28, resulting in activation, while binding CTLA-4 may downregulate activation (2). CD86 engagement with CD28 presentation on T cells results in DC activation and triggering of key pro-inflammatory cytokine responses through NF-kB. Studies suggest differentiable roles of CD86 and CD80 in adaptive inflammatory responses through in vivo studies such as in graft rejection (3). Additional studies also indicate different roles of both CD86 and CD80 in innate immune responses such as in sepsis (4). In the latter study it appears that sepsis and initial septic shock, a pro-inflammatory cytokine storm notably comprised of IL-6, TNFa, and IL-1b, is associated with CD80 regulation, while CD86 contribution at least in this model was not as prominent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB140
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
MAB342
Species: Hu
Applications: WB, AgAct
MAB1774
Species: Hu
Applications: Flow, CyTOF-ready, ICC
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
796-IC
Species: Mu
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF4885
Species: Mu
Applications: WB, IP
NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for B7-2/CD86 Antibody (NBP2-25208) (0)

There are no publications for B7-2/CD86 Antibody (NBP2-25208).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for B7-2/CD86 Antibody (NBP2-25208) (0)

There are no reviews for B7-2/CD86 Antibody (NBP2-25208). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for B7-2/CD86 Antibody (NBP2-25208) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional B7-2/CD86 Products

Bioinformatics Tool for B7-2/CD86 Antibody (NBP2-25208)

Discover related pathways, diseases and genes to B7-2/CD86 Antibody (NBP2-25208). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for B7-2/CD86 Antibody (NBP2-25208)

Discover more about diseases related to B7-2/CD86 Antibody (NBP2-25208).
 

Pathways for B7-2/CD86 Antibody (NBP2-25208)

View related products by pathway.

PTMs for B7-2/CD86 Antibody (NBP2-25208)

Learn more about PTMs related to B7-2/CD86 Antibody (NBP2-25208).
 

Research Areas for B7-2/CD86 Antibody (NBP2-25208)

Find related products by research area.

Blogs on B7-2/CD86.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our B7-2/CD86 Antibody (BU63) and receive a gift card or discount.

Bioinformatics

Gene Symbol CD86
Entrez
Uniprot