CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and ...read more
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Alternate Names for CpG oligodeoxynucleotides with negative control, TLR9 ligand
CpG oligodeoxynucleotides with negative control oligo, TLR9 ligand (human)
Synthetic oligodeoxynucleotides (ODN) containing unmethylated deoxycytosine-deoxyguanosine (CpG) motifs. These CpG motifs are present at a 20 fold greater frequency in bacterial DNA than mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9). Two types of CpG ODNs have been identified based on their distinct activity on plasmacytoid dendritic cells (pDC), which are the key sensors of the CpG motifs. CpG Type A induces IFN-a production in pDC whereas Type B promotes survival, activation and maturation of pDC. The CpG ODN sequence differs between human and mouse, however both negative controls contain GpC instead of CpG.
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Publications for CpG oligodeoxynucleotides with negative control, TLR9 ligand (NBP2-26232)(4)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 2 applications: Flow-IC, In vitro.
FAQs for CpG oligodeoxynucleotides with negative control, TLR9 ligand (NBP2-26232). (Showing 1 - 1 of 1 FAQs).
I worked with your product NBP2-26232 (CpG ODN (2006)) on pDCs. In the data sheet, there is no record whether this CpG is type A or type B so I was wondering if you can clearly this for me. In addition, I wanted to know how this specific CpG is expected to influence the maturation status of pDCs?
Thank you for your inquiry about the CpG ODN (NBP2-26232). This sequence is Type B as shown in the chart on CpG ODN (NBP2-26238). As denoted on the data sheets, ODNs are synthetic forms of oligonucleotides, which contain unmethylated CpG motifs, and can induce cellular immune responses through the activation of Toll-like receptor 9 (TLR9). According to the published literature, Type B promotes survival, activation and maturation of pDC. We quality control the ODNs using our TLR9 stable cell lines as shown on the data sheets (Validation of CpG using the TLR9/HEK 293 cell line). There is considerable published literature regarding ODNs and the maturation of dendritic cells, for examples see these links (type b odn dendritic maturation & 2006 cpg odn dendritic). I would also encourage you consult the literature for additional information about this topic for example using the key words in the scholar.google.com that are most relevant to your model system.