TLR4 Antibody

Images

 
Western Blot: TLR4 Antibody [NB100-56580] - Analysis of TLR4 using this antibody. 1 ug/lane partial recombinant mouse TLR4 protein (tested at 2 ug/ml) and B) RAW cell lysate and C) Daudi cell lysate (tested at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: TLR4 Antibody [NB100-56580] - Analysis of TLR4 in mouse kidney tissue using TLR4 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TLR4 Antibody Summary

Immunogen
This antibody was developed against a sythetic peptide corresponding to amino acids in a range between 30-80 of mouse TLR4.
Specificity
This antibody was tested against partial recombinant mouse TLR4 (extracellular portion), and a 75-80 kDa band was observed. Full-length TLR4 is observed at approximately 90 kD. 2. Zager et al (2006, Fig. 10) observed TLR4 as an ~95 kDa doublet which is thought to represent different degrees of glycosylation. See Product Citation (Renal Physiol 291:F546-F556) for additional information. 3. Presumptive TLR4 30 and 60 kDa cleavage fragments are described in Zagar et al 2006.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 2 - 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This antibody was tested against partial recombinant mouse TLR4 (extracellular portion), and a 75-80 kDa band was observed. Full-length TLR4 is observed at approximately 90 kDa. Zager et al. 2006 (PMID: 16638912) observed TLR4 as an ~95 kDa doublet which is thought to represent different degrees of glycosylation. Presumptive TLR4 30 and 60 kDa cleavage fragments are described in Zagar et al 2007 (PMID: 16885150).
Agonist
Antagonist
Reviewed Applications
Read 1 Review rated 3
using
NB100-56580 in the following applications:

Publications
Read Publications using
NB100-56580 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22427516)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR4 Antibody

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • TLR4
  • TOLL
  • toll-like receptor 4

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90 kDa (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR4 Antibody (NB100-56580)(19)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate (Rhesus monkey).

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


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ICC/IF
(2)
IHC-P
(1)
WB
(9)
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Human
(4)
Mouse
(7)
Rat
(1)
Primate (Rhesus monkey)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-56580 Applications Species
Koh GY, Kane A, Lee K et al. Parabacteroides distasonis attenuates toll-like receptor 4 signaling and Akt activation and blocks colon tumor formation in high-fat diet-fed azoxymethane-treated mice Int. J. Cancer Apr 26 2018 [PMID: 29696632] (WB, Mouse) WB Mouse
Perconti G, Maranto C, Romancino DP et al. Pro-invasive stimuli and the interacting protein Hsp70 favour the route of alpha-enolase to the cell surface. Sci Rep. 2017 Jun 19 [PMID: 28630480] (ICC/IF, Human) ICC/IF Human
Compare D, Rocco A, Coccoli P et al. Lactobacillus casei DG and its postbiotic reduce the inflammatory mucosal response: an ex-vivo organ culture model of post-infectious irritable bowel syndrome. BMC Gastroenterol. Apr 14 2017 [PMID: 28410580] (WB, Human) WB Human
Deiuliis JA, Syed R, Duggineni D et al. Visceral Adipose MicroRNA 223 Is Upregulated in Human and Murine Obesity and Modulates the Inflammatory Phenotype of Macrophages. PLoS ONE. Nov 4 2016 [PMID: 27812198] (WB, Mouse) WB Mouse
Oyama JI, Shiraki A, Nishikido T et al. EGCG, a green tea catechin, attenuates the progression of heart failure induced by the heart/muscle-specific deletion of MnSOD in mice J Cardiol 2016 Jun 30 [PMID: 27374189] (WB, Human) WB Human
Merkowsky K, Sethi RS, Gill JP, Singh B. Fipronil induces lung inflammation in vivo and cell death in vitro. J Occup Med Toxicol. 2016 Mar 21 [PMID: 26997970] (IHC-P, Mouse) IHC-P Mouse
Pahwa R, Devaraj S, Jialal I. The effect of the accessory proteins, soluble CD14 and lipopolysaccharide-binding protein on Toll-like receptor 4 activity in human monocytes and adipocytes. Int J Obes (Lond) 2016 Jun [PMID: 26880233]
Riehl TE, Santhanam S, Foster L et al. CD44 and TLR4 mediate hyaluronic acid regulation of Lgr5+ stem cell proliferation, crypt fission, and intestinal growth in postnatal and adult mice. Am J Physiol Gastrointest Liver Physiol 2015 Dec 1 [PMID: 26505972] (Mouse) Mouse
Duan H, Qu L, Shou C. Mycoplasma hyorhinis induces epithelial-mesenchymal transition in gastric cancer cell MGC803 via TLR4-NF-kappaB signaling. Cancer Lett 2014 Nov 28 [PMID: 25149064]
Balamurugan Kuppusamy, Sharan Shikha, Klarmann Kimberly D et al. FBXW7alpha attenuates inflammatory signalling by downregulating C/EBPdelta and its target gene Tlr4. Nat Commun. 2013 [PMID: 23575666]
Show All 19 Publications.

Review for TLR4 Antibody (NB100-56580) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56580:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot TLR4 NB100-56580
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reviewed by:
Anonymous
WB Human 09/06/2017
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Summary

ApplicationWestern Blot
Sample TestedNeutrophilic cells whole cell lysate
SpeciesHuman
LotAB011304B-13

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR4 Antibody (NB100-56580) (0)

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Secondary Antibodies

 

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Blogs on TLR4. Showing 1-10 of 11 blog posts - Show all blog posts.

PAMPs and DAMPs: What is the same and what is different about these molecules?
By Victoria OsinskiWhat are PAMPs and DAMPsInflammation results from stimuli signaling damage or infection. The inflammatory response can be beneficial or harmful depending on the type and duration of stimuli. The s...  Read full blog post.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

Lipopolysaccharide from gut microbiome localizes in human atherosclerotic plaques and promotes TLR4-mediated oxidative stress
By Jamshed Arslan, Pharm. D., PhD. Atherosclerosis is a chronic inflammatory condition in which plaques of fats and other substances slowly buildup on the inner walls of arteries to restrict blood flow. In atheroscle...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Recent Reviews

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Anonymous
09/06/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol TLR4
Entrez
Uniprot