TLR4 Antibody


Western Blot: TLR4 Antibody [NB100-56580] - Analysis of TLR4 using this antibody. 1 ug/lane partial recombinant mouse TLR4 protein (tested at 2 ug/ml) and B) RAW cell lysate and C) Daudi cell lysate (tested at 5 ug/ml more
Immunohistochemistry-Paraffin: TLR4 Antibody [NB100-56580] - Analysis of TLR4 in mouse kidney tissue using TLR4 antibody at 5 ug/ml.

Product Details

Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC-P
0.5 mg/ml

Order Details

TLR4 Antibody Summary

This antibody was developed against a sythetic peptide corresponding to amino acids in a range between 30-80 of mouse TLR4.
This antibody was tested against partial recombinant mouse TLR4 (extracellular portion), and a 75-80 kDa band was observed. Full-length TLR4 is observed at approximately 90 kD. 2. Zager et al (2006, Fig. 10) observed TLR4 as an ~95 kDa doublet which is thought to represent different degrees of glycosylation. See Product Citation (Renal Physiol 291:F546-F556) for additional information. 3. Presumptive TLR4 30 and 60 kDa cleavage fragments are described in Zagar et al 2006.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified


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NB100-56580 in the following applications:

Reactivity Notes

Rhesus Monkey. Rat reactivity reported in scientific literature (PMID: 22427516)

Alternate Names for TLR4 Antibody

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • TOLL
  • toll-like receptor 4


The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90 kDa (6).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR4 Antibody (NB100-56580)(13)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate (Rhesus monkey).

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

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Primate (Rhesus monkey)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-56580 Applications Species
Oyama JI, Shiraki A, Nishikido T et al. EGCG, a green tea catechin, attenuates the progression of heart failure induced by the heart/muscle-specific deletion of MnSOD in mice J Cardiol 2016 Jun 30 [PMID:27374189] (WB, Human) WB Human
Merkowsky K, Sethi RS, Gill JP, Singh B. Fipronil induces lung inflammation in vivo and cell death in vitro. J Occup Med Toxicol. 2016 Mar 21 [PMID:26997970] (IHC-P, Mouse) IHC-P Mouse
Riehl TE, Santhanam S, Foster L et al. CD44 and TLR4 mediate hyaluronic acid regulation of Lgr5+ stem cell proliferation, crypt fission, and intestinal growth in postnatal and adult mice. Am J Physiol Gastrointest Liver Physiol 2015 Dec 1 [PMID:26505972] (Mouse) Mouse
Balamurugan Kuppusamy, Sharan Shikha, Klarmann Kimberly D et al. FBXW7alpha attenuates inflammatory signalling by downregulating C/EBPdelta and its target gene Tlr4. Nat Commun. 2013 [PMID:23575666]
Yokoyama R, Itoh S, Kamoshida G et al. Staphylococcal superantigen-like protein 3 binds to the Toll-like receptor 2 extracellular domain and inhibits cytokine production induced by Staphylococcus aureus, cell wall component, or lipopeptides in murine macrophages. Infect Immun. 2012 Aug [PMID:22665377]
Oyama Jun-ichi, Maeda Toyoki, Sasaki Makoto et al. Repetitive hyperthermia attenuates progression of left ventricular hypertrophy and increases telomerase activity in hypertensive rats. Am J Physiol Heart Circ Physiol. 2012 May 15 [PMID:22427516] (Rat) Rat
Tsukamoto H, Fukudome K, Takao S et al. Lipopolysaccharide-binding protein-mediated Toll-like receptor 4 dimerization enables rapid signal transduction against lipopolysaccharide stimulation on membrane-associated CD14-expressing cells. Int Immunol. 2010 Apr [PMID:20133493] (WB) WB
Bernardino AL, Myers TA, Alvarez X et al. Toll-like receptors: insights into their possible role in the pathogenesis of lyme neuroborreliosis. Infect Immun. 2008 Oct [PMID:18694963] (ICC/IF, Primate (Rhesus monkey)) ICC/IF Primate (Rhesus monkey)
Shimada M, Yanai Y, Okazaki T et al. Hyaluronan fragments generated by sperm-secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization. Development. 2008 Jun [PMID:18434414]
Gupta N, Su X, Popov B et al. Intrapulmonary delivery of bone marrow-derived mesenchymal stem cells improves survival and attenuates endotoxin-induced acute lung injury in mice. J Immunol. 2007 Aug 1 [PMID:17641052] (WB, Mouse) WB Mouse
Show All 13 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Blogs on TLR4.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR4: Playing Roles in Apoptosis and Autoimmunity
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor (TLR) family that plays a key role in pathogen recognition and activation of innate immunity. Scientists have found that TLRs are highly conserved from Drosophila to humans, with a high...  Read full blog post.

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Product PDFs


Gene Symbol TLR4

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