HMGB1/HMG-1 Antibody

Images

 
Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Total protein from SHSY-5Y, MCF7, Neuro2A and HeLa was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody [NB100-2322] - HMGB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NB100-2322] - Rabbit blood vessel. Image from verified customer review.
Flow (Intracellular): HMGB1/HMG-1 Antibody [NB100-2322] - An intracellular stain was performed on HeLa with HMGB1/HMG-1 Antibody NB100-2322 and a matched isotype control. Cells were fixed with 4% PFA and then ...read more
Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Detection of HMGB1 in whole cell HeLa lysate using NB100-2322. ECL exposure, 1 min.
Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Hepatocyte protein lysate at 1:1000 4C overnight. Image from verfified customer review.
Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody [NB100-2322] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody [NB100-2322] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody [NB100-2322] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NB100-2322] - Staining of HMGB1 in mouse liver using NB100-2322.
Simple Western: HMGB1/HMG-1 Antibody [NB100-2322] - Simple Western lane view shows a specific band for HMGB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
ELISA: HMGB1/HMG-1 Antibody [NB100-2322] - A dose-dependent titration of the HRP conjugated anti-HMGB1 antibody (Catalog Number: NB100-2322H) on recombinant human HMGB1 protein. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Rb, Sh, Po, EqSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

HMGB1/HMG-1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]
Localization
Nuclear
Predicted Species
Porcine (100%), Equine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMGB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0 ug/ml
  • Simple Western 1:2000
  • ELISA
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 0.05 ug/ml
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Flow (Intracellular)
Application Notes
This HMGB1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen approx. 29 kDa. It has been reported that the HRP conjugated format (Catalog# NB100-2322H) of this antibody works well for use in ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HMGB1/HMG-1 Lysate (NBL1-11614)
HeLa Lysate (NB800-PC1)
HMGB1/HMG-1 Lysate (NBP2-65057)
Control Peptide
HMGB1/HMG-1 Peptide (NB100-2322PEP)
Reviewed Applications
Read 4 Reviews rated 4.5
using
NB100-2322 in the following applications:

Publications
Read Publications using
NB100-2322 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 27401639). Predicted to react with porcine, and equine based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Glycine
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HMGB1/HMG-1 Antibody

  • Amphoterin
  • high mobility group box 1
  • High mobility group protein 1
  • high mobility group protein B1
  • high-mobility group (nonhistone chromosomal) protein 1
  • high-mobility group box 1
  • HMG1
  • HMG-1
  • HMG1DKFZp686A04236
  • HMG3
  • HMGB1
  • SBP-1
  • Sulfoglucuronyl carbohydrate binding protein

Background

HMGB1 (high mobility group protein B1) is a member of HMGB family and it has been extensively studied as a transcription/growth factor that is most ubiquitous and evolutionarily conserved protein in eukaryotic organisms. HMGB1 is localized in nucleus, however gets translocated to cytoplasm or may get secreted also. It is a DNA binding protein that preferentially binds with ssDNA and contains 2 HMG box DNA-binding domains with ability of chromatin association for bending DNA. HMGB1 implicates in in V(D)J recombination by acting as a cofactor of RAG complex that contains core components RAG1 and RAG2, as well as an associated component HMGB1 or HMGB2. HMGB1 acts by stimulating cleavage and RAG protein binding at 23bp spacer of conserved RSS (recombination signal sequences). Originally described as a nonhistone nuclear protein with transcriptional regulatory properties, HMGB1 (especially the cytoplasmic/secreted form) has also now been recognized as a late mediator in septic shock as well as a pro-inflammatory cytokine. It gets translocated to cytoplasm or secreted when cells get exposed to inflammatory mediators (LPS, TNF-alpha, IFN-gamma) or via passive process during cellular necrosis. HMGB1 has been implicated in the pathogenesis of various inflammatory and autoimmune diseases, such as SLE, RA and ankylosing spondylitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HMGB1/HMG-1 Antibody (NB100-2322)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Rabbit.

We have publications tested in 6 applications: ELISA, FLOW, Flow, ICC/IF, IHC, WB.


Filter By Application
ELISA
(1)
FLOW
(2)
Flow
(1)
ICC/IF
(3)
IHC
(3)
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(5)
Bovine
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB100-2322 Applications Species
Kim SY, Son M, Lee SE et al. High-Mobility Group Box 1-Induced Complement Activation Causes Sterile Inflammation. Front Immunol Apr 11 2018 [PMID: 29696019] (WB, Mouse) WB Mouse
Coleman LG, Zou J, Crews FT. Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7 J Neuroinflammation Jan 25 2017 [PMID: 28118842] (FLOW, ELISA, Human). J Neuroinflammation. 2017 Jan 25 [PMID: 28118842] (Flow, ELISA, Human)

Details:
This citation used the Alexa Fluor 488 version of this antibody.
Flow, ELISA Human
Zhou M, Wen Z, Cheng F et al. Tumor-released autophagosomes induce IL-10-producing B cells with suppressive activity on T lymphocytes via TLR2-MyD88-NF-kB signal pathway OncoImmunology 2016 May 13 [PMID: 27622036] (FLOW)

Details:
This reference used the Alexa Fluor 647 version of NB100-2322.
FLOW
Zhou M, Wen Z, Cheng F et al. Tumor-released autophagosomes induce IL-10-producing B cells with suppressive activity on T lymphocytes via TLR2-MyD88-NF-kB signal pathway. OncoImmunology. 2016 May 13 [PMID: 27622036] (FLOW)

Details:
This reference used the Alexa Fluor 647 version of NB100-2322.
FLOW
Kim JS, Lee SG, Oh J et al. Development of Advanced Atherosclerotic Plaque by Injection of Inflammatory Proteins in a Rabbit Iliac Artery Model Yonsei Med. J. 2016 Sep 01 [PMID: 27401639] (IHC, Rabbit) IHC Rabbit
Feng Y, Yang S, Ma Y et al. Role of Toll-like receptors in diabetic renal lesions in a miniature pig model. Sci Adv 2015 Jun [PMID: 26601192] (IHC, Bovine) IHC Bovine
Zahedi K, Barone S, Wang Y et al. Proximal tubule epithelial cell specific ablation of the spermidine/spermine n1-acetyltransferase gene reduces the severity of renal ischemia/reperfusion injury. PLoS OnE. 2014 Nov 13 [PMID: 25390069] (WB, Human) WB Human
Chi-Cheng Lu, Bo-Ru Huang, Pei-Jyuan Liao, Gow-Chin Yen. Ursolic acid triggers nonprogrammed death (necrosis) in human glioblastoma multiforme DBTRG-05MG cells through MPT pore opening and ATP decline. Mol Nutr Food Res. 2014 Nov [PMID: 25131308]
Robinson AP, Caldis MW, Harp CT et al. High-mobility group box 1 protein (HMGB1) neutralization ameliorates experimental autoimmune encephalomyelitis J Autoimmun 2013 Mar 17 [PMID: 23514872] (ICC/IF, Mouse) ICC/IF Mouse
Kang R, Tang D, Schapiro NE et al. The HMGB1/RAGE inflammatory pathway promotes pancreatic tumor growth by regulating mitochondrial bioenergetics. Oncogene 2013 Jan 14 [PMID: 23318458] (WB, Mouse) WB Mouse
Show All 15 Publications.

Reviews for HMGB1/HMG-1 Antibody (NB100-2322) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB100-2322:
Filter by Applications
WB
(3)
Simple Western
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot HMGB1/HMG-1 NB100-2322
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reviewed by:
Anonymous
WB Rat 03/28/2018
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Summary

ApplicationWestern Blot
Sample TestedBrain homogenate
SpeciesRat
LotQ-1

Comments

CommentsBlocked in 5% milk + 1% BSA in TBS for 2 h, incubated overnight in 1:500 HMGB1 antibody at 4 degrees C and then 2 h at room temperature with HRP-conjugated secondary.
Western Blot HMGB1/HMG-1 NB100-2322
Enlarge
reviewed by:
Liyong Zhang
WB Human 11/25/2017
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Summary

ApplicationWestern Blot
Sample Testedmouse hepatocytes and HepG2 cells
SpeciesHuman
LotF-1
Simple Western HMGB1/HMG-1 NB100-2322
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reviewed by:
Anonymous
Simple Western Mouse 12/30/2015
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Summary

ApplicationSimple Western
Sample TestedMouse serum
SpeciesMouse

Blocking

Blocking DetailsDefault settings of ProteinSimple Simple Western assay

Primary Anitbody

Dilution Ratio1:30 and Default settings of ProteinSimple Simple Western assay

Secondary Antibody

Secondary DescriptionGoat anti-rabbit HRP
Secondary Manufacturer Cat#042-206

Details

Detection NotesProtein Simple WES system

Comments

CommentsProtein concentration of 1.5 ug/ul
Western Blot HMGB1/HMG-1 NB100-2322
Enlarge
reviewed by:
Liyong Zhang
WB Mouse 06/26/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse
FileView PDF

Product General Protocols

View specific protocols for HMGB1/HMG-1 Antibody (NB100-2322): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HMGB1/HMG-1 Antibody (NB100-2322). (Showing 1 - 1 of 1 FAQs).

  1. I'd like to use NB100-2322 for FACS. One of the images shows successful staining. Which permeabilization method and fixative was used? I've seen conflicting reports, especially for fixation.
    • This image was obtained using 10% formalin as fixative and 90% methanol for permeabilization.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2322AF405
Alexa Fluor 488 Labeled NB100-2322AF488
Alexa Fluor 647 Labeled NB100-2322AF647
Alexa Fluor 700 Labeled NB100-2322AF700
Biotin Labeled NB100-2322B
DyLight 405 Labeled NB100-2322V
DyLight 488 Labeled NB100-2322G
DyLight 550 Labeled NB100-2322R
DyLight 650 Labeled NB100-2322C
FITC Labeled NB100-2322F
HRP Labeled NB100-2322H
PE Labeled NB100-2322PE

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Bioinformatics Tool for HMGB1/HMG-1 Antibody (NB100-2322)

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Diseases for HMGB1/HMG-1 Antibody (NB100-2322)

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Pathways for HMGB1/HMG-1 Antibody (NB100-2322)

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PTMs for HMGB1/HMG-1 Antibody (NB100-2322)

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

Anonymous
03/28/2018
Application: WB
Species: Rat

Liyong Zhang
11/25/2017
Application: WB
Species: Human

Anonymous
12/30/2015
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol HMGB1
Entrez