TRIF/TICAM1 Antibody

Images

 
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse spleen probed with TRIF antibody, in A: presence and B: absence of immunizing peptide, at 1:1000.
Immunocytochemistry/ Immunofluorescence: TRIF/TICAM1 Antibody [NB120-13810] - TRIF antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse A: Intestine. B: Spleen. C: RAW lysate using TRIF antibody at 1:1000 dilution.
Simple Western: TRIF/TICAM1 Antibody [NB120-13810] - Image shows a specific band for TRIF in 0.5 mg/mL of Raji lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Simple Western, IB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TRIF/TICAM1 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the mouse TRIF protein (between amino acids 100-180) [UniProt Q80UF7].
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TICAM1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:100
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:10-1:100
  • Immunoprecipitation
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunoprecipitation reported in scientific literature (PMID 28007523). Use in Immunoblotting reported in scientific literature (PMID 27043414)..
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)
TRIF/TICAM1 Overexpression Lysate
Publications
Read Publications using
NB120-13810 in the following applications:

  • IB
    1 publication
  • 1 publication
  • IP
    4 publications
  • WB
    21 publications

Reactivity Notes

Human reactivity reported in the scientific literature (PMID: 23777661). Rat reactivity reported in scientific literature (PMID: 23447644). Porcine reactivity reported in scientific literature (PMID: 27046485).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for TRIF/TICAM1 Antibody

  • MGC35334
  • Proline-rich, vinculin and TIR domain-containing protein B
  • PRVTIRBTIR domain-containing adapter protein inducing IFN-beta
  • Putative NF-kappa-B-activating protein 502H
  • TICAM1
  • TICAM-1TIR domain containing adaptor inducing interferon-beta
  • TIR domain-containing adapter molecule 1
  • TIR domain-containing adaptor molecule 1
  • toll-like receptor adaptor molecule 1
  • TRIF
  • TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta

Background

TICAM (Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule-1) or TRIF (Toll/IL-1 receptor domain-containing adapter inducing IFN-beta) plays an essential role in the MyD88-independent signaling of TLR3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRIF/TICAM1 Antibody (NB120-13810)(28)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 4 applications: IB, ICC/IF, IP, WB.


Filter By Application
IB
(1)
ICC/IF
(1)
IP
(4)
WB
(21)
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Filter By Species
Human
(3)
Mouse
(12)
Rat
(3)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NB120-13810 Applications Species
Feng Z, Yang R, Wu L et al. Atractylodes macrocephala polysaccharides regulate the innate immunity of colorectal cancer cells by modulating the TLR4 signaling pathway Onco Targets Ther [PMID: 31564895] (Mouse) Mouse
Zhou S, Qi Q, Wang X, et al. SjHSP60 induces CD4+ CD25+ Foxp3+ Tregs via TLR4-Mal-drived production of TGF-beta in macrophages. Immunol Cell Biol. 2018 May 21 [PMID: 29697865] (WB, Mouse) WB Mouse
Gao T, Zhang SP, Wang JF et al. TLR3 contributes to persistent autophagy and heart failure in mice after myocardial infarction J. Cell. Mol. Med. 2017 Sep 25 [PMID: 28945004] (WB, Mouse) WB Mouse
Popiolek-Barczyk K, Piotrowska A, Makuch W, Mika J. Biphalin, a Dimeric Enkephalin, Alleviates LPS-Induced Activation in Rat Primary Microglial Cultures in Opioid Receptor-Dependent and Receptor-Independent Manners.. Neural Plast. Jun 2 2017 [PMID: 28573049] (WB, Rat) WB Rat
Kim E, Beon J, Lee S et al. Inositol polyphosphate multikinase promotes Toll-like receptor-induced inflammation by stabilizing TRAF6. Sci Adv. Apr 1 2017 [PMID: 28439546] (IP, Mouse) IP Mouse
Lan X, Han X, Li Q et al. Pinocembrin protects hemorrhagic brain primarily by inhibiting toll-like receptor 4 and reducing M1 phenotype microglia Brain Behav. Immun. Dec 20 2016 [PMID: 28007523] (IP, Mouse) IP Mouse
Zhang L, Xiang W, Wang G et al. IFN-beta Production During the dsRNA Response in Hepatocytes Involves Coordinated and Feed Forward Signaling Through TLR3, PkR, iNOS and Src. J. Biol. Chem. May 17 2016 [PMID: 27226571] (WB, Mouse) WB Mouse
Hu Y, Cong X, Chen L et al. Synergy of TLR3 and 7 ligands significantly enhances function of DCs to present inactivated PRRSV antigen through TRIF/MyD88-NF-kB signaling pathway. Sci Rep. 2016 Apr 06 [PMID: 27046485] (WB, Porcine) WB Porcine
Wang F, Alain T, Szretter KJ et al. S6K-STING interaction regulates cytosolic DNA-mediated activation of the transcription factor IRF3. Nat. Immunol. May 1 2016 [PMID: 27043414] (IB, IP, Mouse) IB, IP Mouse
Ma B, Yu J, Xie C et al. Toll-Like Receptors Promote Mitochondrial Translocation of Nuclear Transcription Factor Nuclear Factor of Activated T-Cells in Prolonged Microglial Activation. J. Neurosci. 2015 Jul 29 [PMID: 26224862] (ICC/IF, Mouse)

Details:
TRIF/TICAM1 antibody (Imgenex IMG-661) was used for ICC-IF analysis of Trif siRNA transfected primary microglial cell cultures that were isolated from Nfat1 knock-out mice pups. The assay involved 4% PFA fixation, permeabilization with Triton X-100, blocking with 10% donkey serum, use of primary antibody at 1:500 dilution and detection using Alexa labelled secondary antibody (Figure 7A).
ICC/IF Mouse
Show All 28 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRIF/TICAM1 Antibody (NB120-13810). (Showing 1 - 1 of 1 FAQs).

  1. Regarding the WB testing for the TRIF/TICAM1 Antibody Cat# NB120-13810, can you confirm if the RAW lysate was treated in any way? Some of the antibody references on the product page indicate samples treated with LPS. Is there also any explanation for the molecular weight observed? The signal on mouse is right above the 66 kDa maker while the testing on Raji displays a band closer to 100 kDa.
    • The RAW lysate has not been treated in the western shown here. The difference in sizes can be explained as the mouse sample that is running right at 66 KD is a regular Western Blot, however, the band that is running at 100 KD is on the Simple Western system and tends to run larger than on a regular western blot. Protein Simple is the new line of proteins analysis technology:

Control Lysate(s)

Secondary Antibodies

 

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Blogs on TRIF/TICAM1.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

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