Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Total protein from Raw264.7 cells and mouse spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunohistochemistry-Paraffin: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of a FFPE tissue section of mouse spleen using 1:200 dilution of TRIF/TICAM1 antibody. The staining was developed using HRP labeled ...read more
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse A: Intestine. B: Spleen. C: RAW lysate using TRIF antibody at 1:1000 dilution.
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse spleen probed with TRIF antibody, in A: presence and B: absence of immunizing peptide, at 1:1000.
Genetic Strategies: Knockdown Validated: TRIF/TICAM1 Antibody [NB120-13810] - siRNA was transfected at 50 nM for 48 hrs, and poly(I:C) was added at 100 ug/ml for 4 hrs before cell harvest. Negative control (NC) ...read more
TRIF/TICAM1 Antibody [NB120-13810] - Lysates of heart tissue were immunoprecipitated with anti-TLR3 antibodies (IP: TLR3), followed by SDS-PAGE and immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: ...read more
Immunoblotting reported in scientific literature (PMID 27043414)
Immunoprecipitation reported in scientific literature (PMID 28007523)
Simple Western 1:100
Western Blot 1 - 2 ug/ml
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
76 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta
TICAM (Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule-1) or TRIF (Toll/IL-1 receptor domain-containing adapter inducing IFN-beta) plays an essential role in the MyD88-independent signaling of TLR3.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for TRIF/TICAM1 Antibody (NB120-13810). (Showing 1 - 1 of 1 FAQs).
Regarding the WB testing for the TRIF/TICAM1 Antibody Cat# NB120-13810, can you confirm if the RAW lysate was treated in any way? Some of the antibody references on the product page indicate samples treated with LPS. Is there also any explanation for the molecular weight observed? The signal on mouse is right above the 66 kDa maker while the testing on Raji displays a band closer to 100 kDa.
The RAW lysate has not been treated in the western shown here. The difference in sizes can be explained as the mouse sample that is running right at 66 KD is a regular Western Blot, however, the band that is running at 100 KD is on the Simple Western system and tends to run larger than on a regular western blot. Protein Simple is the new line of proteins analysis technology:
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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