TRIF/TICAM1 Antibody - BSA Free

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Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Total protein from Raw264.7 cells and mouse spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunohistochemistry-Paraffin: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of a FFPE tissue section of mouse spleen using 1:200 dilution of TRIF/TICAM1 antibody. The staining was developed using HRP labeled ...read more
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse A: Intestine. B: Spleen. C: RAW lysate using TRIF antibody at 1:1000 dilution.
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse spleen probed with TRIF antibody, in A: presence and B: absence of immunizing peptide, at 1:1000.
Genetic Strategies: Knockdown Validated: TRIF/TICAM1 Antibody [NB120-13810] - siRNA was transfected at 50 nM for 48 hrs, and poly(I:C) was added at 100 ug/ml for 4 hrs before cell harvest. Negative control (NC) ...read more
TRIF/TICAM1 Antibody [NB120-13810] - Lysates of heart tissue were immunoprecipitated with anti-TLR3 antibodies (IP: TLR3), followed by SDS-PAGE and immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: ...read more

Product Details

Summary
Reactivity Mu, Rt, PoSpecies Glossary
Applications WB, Simple Western, IB, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

TRIF/TICAM1 Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to an internal portion of the mouse TRIF protein (between amino acids 100-180) [UniProt Q80UF7].
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TICAM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:10-1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Knockdown Validated
  • Simple Western 1:100
  • Western Blot 1 - 2 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunoprecipitation reported in scientific literature (PMID 28007523). Use in Immunoblotting reported in scientific literature (PMID 27043414)..
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)
TRIF/TICAM1 Overexpression Lysate
Publications
Read Publications using
NB120-13810 in the following applications:

  • IB
    1 publication
  • 1 publication
  • IP
    4 publications
  • WB
    21 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23447644). Porcine reactivity reported in scientific literature (PMID: 27046485).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIF/TICAM1 Antibody - BSA Free

  • MGC35334
  • Proline-rich, vinculin and TIR domain-containing protein B
  • PRVTIRBTIR domain-containing adapter protein inducing IFN-beta
  • Putative NF-kappa-B-activating protein 502H
  • TICAM1
  • TICAM-1TIR domain containing adaptor inducing interferon-beta
  • TIR domain-containing adapter molecule 1
  • TIR domain-containing adaptor molecule 1
  • toll-like receptor adaptor molecule 1
  • TRIF
  • TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta

Background

TICAM (Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule-1) or TRIF (Toll/IL-1 receptor domain-containing adapter inducing IFN-beta) plays an essential role in the MyD88-independent signaling of TLR3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRIF/TICAM1 Antibody (NB120-13810)(28)

We have publications tested in 3 confirmed species: Mouse, Rat, Porcine.

We have publications tested in 4 applications: IB, ICC/IF, IP, WB.


Filter By Application
IB
(1)
ICC/IF
(1)
IP
(4)
WB
(21)
All Applications
Filter By Species
Mouse
(13)
Rat
(4)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NB120-13810 Applications Species
Karmakar J, Mandal C Interplay Between Sialic Acids, Siglec-E, and Neu1 Regulates MyD88- and TRIF-Dependent Pathways for TLR4-Activation During Leishmania donovani Infection Frontiers in immunology Mar 3 2021 [PMID: 33763070] (WB, Mouse) WB Mouse
Chen YL, Shirakawa H, Lu NS et al. Impacts of fish oil on the gut microbiota of rats with alcoholic liver damage J. Nutr. Biochem. Sep 10 2020 [PMID: 32920090] (WB, Rat) WB Rat
Shi D, Chen M, Liu L et al. Anti-influenza A virus mechanism of three representative compounds from Flos Trollii via TLRs signaling pathways J Ethnopharmacol Jan 28 2020 [PMID: 32004628] (WB, Mouse) WB Mouse
Feng Z, Yang R, Wu L et al. Atractylodes macrocephala polysaccharides regulate the innate immunity of colorectal cancer cells by modulating the TLR4 signaling pathway Onco Targets Ther [PMID: 31564895] (Mouse) Mouse
Zhou S, Qi Q, Wang X, et al. SjHSP60 induces CD4+ CD25+ Foxp3+ Tregs via TLR4-Mal-drived production of TGF-beta in macrophages. Immunol Cell Biol. 2018 May 21 [PMID: 29697865] (WB, Mouse) WB Mouse
Gao T, Zhang SP, Wang JF et al. TLR3 contributes to persistent autophagy and heart failure in mice after myocardial infarction J. Cell. Mol. Med. 2017 Sep 25 [PMID: 28945004] (WB, Mouse) WB Mouse
Popiolek-Barczyk K, Piotrowska A, Makuch W, Mika J. Biphalin, a Dimeric Enkephalin, Alleviates LPS-Induced Activation in Rat Primary Microglial Cultures in Opioid Receptor-Dependent and Receptor-Independent Manners.. Neural Plast. Jun 2 2017 [PMID: 28573049] (WB, Rat) WB Rat
Kim E, Beon J, Lee S et al. Inositol polyphosphate multikinase promotes Toll-like receptor-induced inflammation by stabilizing TRAF6. Sci Adv. Apr 1 2017 [PMID: 28439546] (IP, Mouse) IP Mouse
Lan X, Han X, Li Q et al. Pinocembrin protects hemorrhagic brain primarily by inhibiting toll-like receptor 4 and reducing M1 phenotype microglia Brain Behav. Immun. Dec 20 2016 [PMID: 28007523] (IP, Mouse) IP Mouse
Zhang L, Xiang W, Wang G et al. IFN-beta Production During the dsRNA Response in Hepatocytes Involves Coordinated and Feed Forward Signaling Through TLR3, PkR, iNOS and Src. J. Biol. Chem. May 17 2016 [PMID: 27226571] (WB, Mouse) WB Mouse
Show All 28 Publications.

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Product General Protocols

View specific protocols for TRIF/TICAM1 Antibody (NB120-13810): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRIF/TICAM1 Antibody (NB120-13810). (Showing 1 - 1 of 1 FAQs).

  1. Regarding the WB testing for the TRIF/TICAM1 Antibody Cat# NB120-13810, can you confirm if the RAW lysate was treated in any way? Some of the antibody references on the product page indicate samples treated with LPS. Is there also any explanation for the molecular weight observed? The signal on mouse is right above the 66 kDa maker while the testing on Raji displays a band closer to 100 kDa.
    • The RAW lysate has not been treated in the western shown here. The difference in sizes can be explained as the mouse sample that is running right at 66 KD is a regular Western Blot, however, the band that is running at 100 KD is on the Simple Western system and tends to run larger than on a regular western blot. Protein Simple is the new line of proteins analysis technology:

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on TRIF/TICAM1.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

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Bioinformatics

Gene Symbol TICAM1
Entrez
Uniprot