TRIF/TICAM1 Antibody


Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse spleen probed with TRIF antibody, in the A) presence and B) absence of immunizing peptide, at 1:1000.
Immunocytochemistry/ Immunofluorescence: TRIF/TICAM1 Antibody [NB120-13810] - TRIF antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight more
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse A) intestine, B) spleen and C) RAW lysate using TRIF antibody at 1:1000 dilution.
Simple Western: TRIF/TICAM1 Antibody [NB120-13810] - Simple Western lane view shows a specific band for TRIF in 0.5 mg/ml of Raji lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Simple Western, ICC/IF
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

TRIF/TICAM1 Antibody Summary

Synthetic peptide made to an internal portion of the mouse TRIF protein (between amino acids 100-180) [UniProt Q80UF7].
The will cross-react with human TRIF, but additional bands may be seen.
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Whole sera
0.02% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
The ~70 kD band detected by this antibody in western blot, corresponding to TRIF protein, is blocked by the immunizing peptide. In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
76 kDa
Read Publications using
NB120-13810 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    18 publications

Reactivity Notes

Human reactivity reported in the scientific literature (PMID: 23777661). Rat reactivity reported in scientific literature (PMID: 23447644). Porcine reactivity reported in scientific literature (PMID: 27046485).

Alternate Names for TRIF/TICAM1 Antibody

  • MGC35334
  • Proline-rich, vinculin and TIR domain-containing protein B
  • PRVTIRBTIR domain-containing adapter protein inducing IFN-beta
  • Putative NF-kappa-B-activating protein 502H
  • TICAM1
  • TICAM-1TIR domain containing adaptor inducing interferon-beta
  • TIR domain-containing adapter molecule 1
  • TIR domain-containing adaptor molecule 1
  • toll-like receptor adaptor molecule 1
  • TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta


TICAM (Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule-1) or TRIF (Toll/IL-1 receptor domain-containing adapter inducing IFN-beta) plays an essential role in the MyD88-independent signaling of TLR3.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRIF/TICAM1 Antibody (NB120-13810)(21)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 3 applications: ICC/IF, IP, WB.

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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB120-13810 Applications Species
Zhang L, Xiang W, Wang G et al. IFN-beta Production During the dsRNA Response in Hepatocytes Involves Coordinated and Feed Forward Signaling Through TLR3, PkR, iNOS and Src. J. Biol. Chem. May 17 2016 12:00AM [PMID:27226571] (WB, Mouse) WB Mouse
Hu Y, Cong X, Chen L et al. Synergy of TLR3 and 7 ligands significantly enhances function of DCs to present inactivated PRRSV antigen through TRIF/MyD88-NF-kB signaling pathway. Sci Rep. 2016 Apr 06 [PMID:27046485] (WB, Porcine) WB Porcine
Ma B, Yu J, Xie C et al. Toll-Like Receptors Promote Mitochondrial Translocation of Nuclear Transcription Factor Nuclear Factor of Activated T-Cells in Prolonged Microglial Activation. J. Neurosci. 2015 Jul 29 [PMID:26224862] (ICC/IF, Mouse) ICC/IF Mouse
Vogt A, Scull MA, Friling T et al. Recapitulation of the hepatitis C virus life-cycle in engineered murine cell lines. Virology 2013 Jun 15 [PMID:23777661] (WB, Mouse, Human) WB Mouse, Human
Haque A, Koide N, Odkhuu E et al. Mouse pyrin and HIN domain family member 1 (pyhin1) protein positively regulates LPS-induced IFN-B and NO production in macrophages. Innate Immun. 2014 Jan 1 [PMID:23606517] (WB, Mouse) WB Mouse
Tian J, Guo X, Liu XM et al. Extracellular HSP60 induces inflammation through activating and up-regulating TLRs in cardiomyocytes. Cardiovasc Res. 2013 Jun 1 [PMID:23447644] (WB, Rat) WB Rat
Abe T, Hirasaka K, Kagawa S et al. Cbl-b Is a Critical Regulator of Macrophage Activation Associated with Obesity-Induced Insulin Resistance in Mice. Diabetes 2013 Jan 24 [PMID:23349502]
De Luca A, Iannitti RG, Bozza S et al. CD4(+) T cell vaccination overcomes defective cross-presentation of fungal antigens in a mouse model of chronic granulomatous disease. J Clin Invest. 2012 May 1 [PMID:22523066] (WB) WB
Seleme MC, Lei W, Burg AR et al. Dysregulated TLR3-dependent signaling and innate immune activation in superoxide-deficient macrophages from nonobese diabetic mice. Free Radic Biol Med. 2012 May 1 [PMID:22361747] (WB) WB
Zhang Z, Kim T, Bao M et al. DDX1, DDX21, and DHX36 Helicases Form a Complex with the Adaptor Molecule TRIF to Sense dsRNA in Dendritic Cells. Immunity;34(6):866-78. 2011 Jun 24. [PMID:21703541]
Show All 21 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRIF/TICAM1 Antibody (NB120-13810). (Showing 1 - 1 of 1 FAQs).

  1. Regarding the WB testing for the TRIF/TICAM1 Antibody Cat# NB120-13810, can you confirm if the RAW lysate was treated in any way? Some of the antibody references on the product page indicate samples treated with LPS. Is there also any explanation for the molecular weight observed? The signal on mouse is right above the 66 kDa maker while the testing on Raji displays a band closer to 100 kDa.
    • The RAW lysate has not been treated in the western shown here. The difference in sizes can be explained as the mouse sample that is running right at 66 KD is a regular Western Blot, however, the band that is running at 100 KD is on the Simple Western system and tends to run larger than on a regular western blot. Protein Simple is the new line of proteins analysis technology:

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Blogs on TRIF/TICAM1.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

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Gene Symbol TICAM1

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