TLR4 Antibody (HTA125) [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Po, Ca, GP, PmSpecies Glossary
Applications Flow, ICC/IF, IHC, WB (-)
Clone
HTA125
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

TLR4 Antibody (HTA125) [FITC] Summary

Immunogen
This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.
Specificity
The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TLR4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1ul/1 million cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
Confocal Microscopy: see Scheel et al. (2006) for details FA (Neutralization): please Basek et al, 2005 for details. Flow (Cell Surface): 2-5 ug/ 1x10^6 cells IF/ICC: please see Schneeman et al (2005) for details. IP: please see Shimazu et al, 1999 for details. Use in Immunohistochemistry and Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28219705)
Agonist
Antagonist
Publications
Read Publications using
NB100-56059 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for TLR4 Antibody (HTA125) [FITC]

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • TLR4
  • TOLL
  • toll-like receptor 4

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR4 Antibody (NB100-56059)(24)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: FLOW, Flow, Flow-CS, Flow-Cs, Flow-IC, ICC/IF.


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Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-56059 Applications Species
Heftrig D, Sturm R, Oppermann E et al. Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients.. Mediators Inflamm. Mar 3 2017 [PMID: 28255201]
Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J. Allergy Clin. Immunol. Feb 20 2017 [PMID: 28219705] (Flow, Mouse) Flow Mouse
Jamin A, Dehoux L, Dossier C et al. Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome Clin. Exp. Immunol. 2015 Jun 30 [PMID: 26123900] (FLOW, Human)

Details:
TLR4 antibody clone HTA125 [FITC] (Imgenex: IMG-417C) was used for FLOW on peripheral blood mononuclear cells/PBMC of idiopathic nephrotic syndrome/INS children, including 28 in relapse, 23 in remission and 40 controls. The cells were incubated with conjugated antibodies along with isotype control for 30 minutes at 4C and 10(5) viable PBMCs were analyzed using FACSCantoll/FACSDiva.
FLOW Human
Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013 Jan 1 [PMID: 23183897]
Matsunaga N, Tsuchimori N, Matsumoto T, Ii M. TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules. Mol Pharmacol. 2011 Jan [PMID: 20881006]
Pillay J, Ramakers BP, Kamp VM et al. Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia. J Leukoc Biol. 2010 Jul [PMID: 20400675]
Wu CY, Chi PL, Hsieh HL et al. TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2. J Cell Physiol. 2010 May [PMID: 20112284]
Pietschmann K, Beetz S, Welte S et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009 Sep [PMID: 19703014]
O'Hara SP, Small AJ, Gajdos GB et al. HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum. J Infect Dis. 2009 Apr 15 [PMID: 19265483]
Yang X, Fullerton DA, Su X et al. Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2. J Am Coll Cardiol. 2009 Feb 10 [PMID: 19195606]
Show All 24 Publications.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP2-24897
Biotin Labeled NBP2-24744
DyLight 550 Labeled NB100-56727R
DyLight 650 Labeled NB100-56727C
HRP Labeled NB100-56727H
PE Labeled NB100-56062

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Bioinformatics Tool for TLR4 Antibody (NB100-56059)

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Blogs on TLR4.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR4: Playing Roles in Apoptosis and Autoimmunity
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor (TLR) family that plays a key role in pathogen recognition and activation of innate immunity. Scientists have found that TLRs are highly conserved from Drosophila to humans, with a high...  Read full blog post.

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Bioinformatics

Gene Symbol TLR4
Entrez