TLR4 Antibody (HTA125) [PE]

Images

 
Flow Cytometry: TLR4 Antibody (HTA125) [PE] [NB100-56062] - Intracellular flow analysis of TLR4 in human PBMCs using NB100-56062 at 0.5 ug/10^6 cells. The shaded histogram represents cells without anti-TLR4 antibody; ...read more
Flow Cytometry: TLR4 Antibody (HTA125) [PE] [NB100-56062] - Cell surface analysis of TLR4 on ThP1 cells using TLR4 antibody at 2 ug/10^6 cells. The shaded histogram represents ThP1 cells only, green represents isotype ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Ca, GP, PmSpecies Glossary
Applications Flow, WB (-)
Clone
HTA125
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

TLR4 Antibody (HTA125) [PE] Summary

Immunogen
Ba/F3 cell line expressing TLR4
Localization
Type I membrane protein.
Specificity
NB600-662 recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TLR4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1ul/1 million cells
Application Notes
Confocal Microscopy: see Scheel et al. (2006) for details FA (Neutralization): please Basek et al, 2005 for details. Flow (Cell Surface): 2-5 ug/ 1x10^6 cells IF/ICC: please see Schneeman et al (2005) for details. IP: please see Shimazu et al, 1999 for details
Agonist
Antagonist
Publications
Read Publications using
NB100-56062 in the following applications:

Reactivity Notes

Cross-reacts with Human and Guinea Pig.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for TLR4 Antibody (HTA125) [PE]

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • TLR4
  • TOLL
  • toll-like receptor 4

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TLR4 Antibody (NB100-56062)(23)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 5 applications: FLOW, Flow-CS, Flow-Cs, Flow-IC, ICC/IF.


Filter By Application
FLOW
(2)
Flow-CS
(1)
Flow-Cs
(1)
Flow-IC
(1)
ICC/IF
(2)
All Applications
Filter By Species
Human
(3)
Canine
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB100-56062 Applications Species
Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes Veterinary Parasitology Feb 15 2016 [PMID: 26995719] (FLOW, Canine) FLOW Canine
Komine-Aizawa S, Hirohata N, Aizawa S, Abiko Y. Porphyromonas gingivalis lipopolysaccharide inhibits trophoblast invasion in the presence of nicotine Placenta et al. 2014 Nov 07 [PMID: 25468545] (FLOW, Human)

Details:
TLR4 antibody used for FLOW on immortalized human first-trimester trophoblast cell line HTR-8/Svneo treated or not with Porphyromonas gingivalis lipopolysaccharide and nicotine (Fig. 1a and b)
FLOW Human
Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013 Jan 1 [PMID: 23183897]
Matsunaga N, Tsuchimori N, Matsumoto T, Ii M. TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules. Mol Pharmacol. 2011 Jan [PMID: 20881006]
Pillay J, Ramakers BP, Kamp VM et al. Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia. J Leukoc Biol. 2010 Jul [PMID: 20400675]
Wu CY, Chi PL, Hsieh HL et al. TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2. J Cell Physiol. 2010 May [PMID: 20112284]
Pietschmann K, Beetz S, Welte S et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009 Sep [PMID: 19703014]
O'Hara SP, Small AJ, Gajdos GB et al. HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum. J Infect Dis. 2009 Apr 15 [PMID: 19265483]
Yang X, Fullerton DA, Su X et al. Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2. J Am Coll Cardiol. 2009 Feb 10 [PMID: 19195606]
Hammadi A, Billard C, Faussat AM, Kolb JP. Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation. Nitric Oxide. 2008 Sep [PMID: 18474259]
Show All 23 Publications.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP2-24897
Biotin Labeled NBP2-24744
DyLight 550 Labeled NB100-56727R
DyLight 650 Labeled NB100-56727C
FITC Labeled NB100-56059
HRP Labeled NB100-56727H
PE Labeled NB600-728

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Blogs on TLR4.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR4: Playing Roles in Apoptosis and Autoimmunity
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor (TLR) family that plays a key role in pathogen recognition and activation of innate immunity. Scientists have found that TLRs are highly conserved from Drosophila to humans, with a high...  Read full blog post.

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Bioinformatics

Gene Symbol TLR4