Flow Cytometry: TLR4 Antibody (HTA125) [PE] [NB100-56062] - Intracellular flow analysis of TLR4 in human PBMCs using NB100-56062 at 0.5 ug/10^6 cells. The shaded histogram represents cells without anti-TLR4 antibody; ...read more
Flow Cytometry: TLR4 Antibody (HTA125) [PE] [NB100-56062] - Cell surface analysis of TLR4 on ThP1 cells using TLR4 antibody at 2 ug/10^6 cells. The shaded histogram represents ThP1 cells only, green represents isotype ...read more
NB600-662 recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Confocal Microscopy: see Scheel et al. (2006) for details FA (Neutralization): please Basek et al, 2005 for details. Flow (Cell Surface): 2-5 ug/ 1x10^6 cells IF/ICC: please see Schneeman et al (2005) for details. IP: please see Shimazu et al, 1999 for details
Cross-reacts with Human and Guinea Pig.Not yet tested in other species.
Packaging, Storage & Formulations
Store at 4C in the dark.
0.05% Sodium Azide
Protein G purified
Alternate Names for TLR4 Antibody (HTA125) [PE]
hTollhomolog of Drosophila toll
toll-like receptor 4
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The role of TLR4 in breast cancer Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation. In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy... Read full blog post.
TLR4 - A Guardian of Innate Immunity Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of... Read full blog post.
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