TLR4 Antibody (HTA125) - Azide Free

Images

 
Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using this antibody. Secondary ...read more
Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56727. Staining of TLR4 on PBMC using this antibody at 1 ug/10^6 cells. Green represents ...read more
Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56727. Staining of TLR4 in stable HEK293/hTLR4 cells using this antibody at 1ug/10^6 ...read more
Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56727. Staining of TLR4 on PBMC using this antibody at 1 ug/10^6 cells. Green represents ...read more
Flow (Intracellular): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the PE conjugate of NB100-56727. Staining of TLR4 in human PBMCs using NB100-56062 at 0.5 ug/10^6 cells. The shaded histogram ...read more
Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the PE conjugate of NB100-56727. Staining of TLR4 on ThP1 cells using TLR4 antibody at 2 ug/10^6 cells. The shaded histogram ...read more
Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Analysis of TLR4 using NB100-56723 on PBMCs at 2ug/ 1X10^6 cells. M1 gate are unstained PBMC.
Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using NB100-56723 at 1 ug/10^6 cells. ...read more
Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Staining of human peripheral blood monocytes with Mouse anti Human CD284.
Blocking/Neutralizing: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Validation of the neutralizing effect of this antibody on TLR4 activity. The TLR4/MD-2/CD14/IL-8 Prom/LUCPorter HeLa cells were plated in ...read more

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, B/N, Flow, Flow-CS, Flow-IC, ICC/IF, In vitro, IP, CyTOF-ready
Clone
HTA125
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide Free

TLR4 Antibody (HTA125) - Azide Free Summary

Immunogen
This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.
Specificity
The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TLR4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Blocking/Neutralizing
  • Flow Cytometry 1ul/1 million cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:2000
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow (Intracellular)
Application Notes
Confocal Microscopy: see Scheel et al. (2006) for details FA (Neutralization): please Basek et al, 2005 for details. Flow (Cell Surface): 2-5 ug/ 1x10^6 cells IF/ICC: please see Schneeman et al (2005) for details. IP: please see Shimazu et al, 1999 for details Use in vitro assays and blocking/ neutralizing reported in scientific literature (PMID 24512642). Use in Western blot reported in scientific literature (PMID 28546218).
Agonist
Antagonist
Publications
Read Publications using
NB100-56727 in the following applications:

Reactivity Notes

Cross reacts with Human and Dog.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Filter (0.2 micron) sterilized PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for TLR4 Antibody (HTA125) - Azide Free

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • TLR4
  • TOLL
  • toll-like receptor 4

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56722
Species: Hu, Ca
Applications: WB, B/N, Flow, Func, ICC/IF, IHC-P, IP, Flow-CS
NB100-56655
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-29328
Species: Hu, Mu, Rt, Xp, Ye, Ze
Applications: ELISA
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NBP1-88968
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-24729
Species: Hu, Mu, Rt, Ca, Eq, Pm
Applications: WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IHC-P, IP, In vitro, CyTOF-ready, Flow-IC
NBP2-24875
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NB120-13810
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, IB, ICC/IF, IP
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP2-24787
Species: Hu, Mu, Ca
Applications: WB, Flow, IHC, IHC-Fr, IHC-P, Flow-CS, Flow-IC
NB100-2322
Species: Hu, Mu, Rt, Bv, Ca, Rb, Sh
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
Supplier Logo

Publications for TLR4 Antibody (NB100-56727)(28)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: B/N, FLOW, Flow, Flow-CS, ICC/IF, In vitro, WB.


Filter By Application
B/N
(3)
FLOW
(2)
Flow
(3)
Flow-CS
(1)
ICC/IF
(2)
In vitro
(1)
WB
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NB100-56727 Applications Species
Li F, Song R, Ao L et al. ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells. Arterioscler. Thromb. Vasc. Biol. May 25 2017 [PMID: 28546218] (B/N, WB, Human) B/N, WB Human
Kabisch R, Mejias-Luque R, Gerhard M, Prinz C. Involvement of Toll-Like Receptors on Helicobacter pylori-Induced Immunity. PLoS ONE. 2014 Aug 26 [PMID: 25153703] (B/N, Human)

Details:
TLR4 antibody used for neutralization experiments at 5 ug/ml concentration (1 h incubation at 37C) on dendritic cells (DCs) generated from fresh blood samples that were taken from healthy volunteers.
B/N Human
Rojas-Bernabe A, Garcia-Hernandez O, Maldonado-Bernal C et al. Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4. Parasitology 2014 Feb 05 [PMID: 24512642] (In vitro, B/N, Human)

Details:
Human macrophages, Figs 3,6.
In vitro, B/N Human
Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013 Jan 1 [PMID: 23183897]
Matsunaga N, Tsuchimori N, Matsumoto T, Ii M. TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules. Mol Pharmacol. 2011 Jan [PMID: 20881006]
Pillay J, Ramakers BP, Kamp VM et al. Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia. J Leukoc Biol. 2010 Jul [PMID: 20400675]
Wu CY, Chi PL, Hsieh HL et al. TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2. J Cell Physiol. 2010 May [PMID: 20112284]
Pietschmann K, Beetz S, Welte S et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009 Sep [PMID: 19703014]
O'Hara SP, Small AJ, Gajdos GB et al. HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum. J Infect Dis. 2009 Apr 15 [PMID: 19265483]
Hammadi A, Billard C, Faussat AM, Kolb JP. Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation. Nitric Oxide. 2008 Sep [PMID: 18474259]
Show All 28 Publications.

Reviews for TLR4 Antibody (NB100-56727) (0)

There are no reviews for TLR4 Antibody (NB100-56727). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR4 Antibody (NB100-56727) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP2-24897
Biotin Labeled NBP2-24744
DyLight 550 Labeled NB100-56727R
DyLight 650 Labeled NB100-56727C
FITC Labeled NB100-56059
HRP Labeled NB100-56727H
PE Labeled NB600-728
PE Labeled NB100-56062

Additional TLR4 Products

Bioinformatics Tool for TLR4 Antibody (NB100-56727)

Discover related pathways, diseases and genes to TLR4 Antibody (NB100-56727). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TLR4 Antibody (NB100-56727)

Discover more about diseases related to TLR4 Antibody (NB100-56727).
 

Pathways for TLR4 Antibody (NB100-56727)

View related products by pathway.

PTMs for TLR4 Antibody (NB100-56727)

Learn more about PTMs related to TLR4 Antibody (NB100-56727).
 

Research Areas for TLR4 Antibody (NB100-56727)

Find related products by research area.

Blogs on TLR4.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR4: Playing Roles in Apoptosis and Autoimmunity
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor (TLR) family that plays a key role in pathogen recognition and activation of innate immunity. Scientists have found that TLRs are highly conserved from Drosophila to humans, with a high...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TLR4 Antibody (HTA125) - Azide Free and receive a gift card or discount.

Bioinformatics

Gene Symbol TLR4
Entrez