TLR4 Antibody (HTA125)

Images

 
Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide/BSA FREE version of NB100-56727. Staining of human peripheral blood monocytes with Mouse anti Human CD284.
Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using this antibody. Secondary antibody goat ...read more
Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis of PE conjugate of NB100-56723. Cell surface TLR4 on ThP1 cells using TLR4 antibody at 2 ug/10^6 cells. The shaded histogram represents ThP1 cells only, ...read more
Biological Strategies: Blocking/Neutralizing: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide Free version of NB100-56723. Validation of the neutralizing effect of this antibody on TLR4 activity. The ...read more
Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using the PE conjugate of NB100-56727. Staining of TLR4 in human PBMCs using NB100-56062 at 0.5 ug/10^6 cells. The shaded histogram represents cells ...read more
Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using NB100-56723 at 1 ug/10^6 cells. Secondary ...read more
Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Staining of U937 cells with MOUSE ANTI HUMAN CD284:RPE. Image using the PE form of this antibody.
Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide/BSA FREE version of NB100-56727. Analysis of TLR4 using NB100-56723 on PBMCs at 2ug/ 1X10^6 cells. M1 gate are unstained PBMC.
Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56727. Staining of TLR4 in stable HEK293/hTLR4 cells using this antibody at 1ug/10^6 cells. Green ...read more
Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56727. Staining of TLR4 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control ...read more

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, Flow, ICC/IF, In vitro, IP, B/N, CyTOF-ready
Clone
HTA125
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

TLR4 Antibody (HTA125) Summary

Immunogen
This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.
Specificity
The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TLR4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • Block/Neutralize
  • CyTOF-ready
Application Notes
Confocal Microscopy: see Scheel et al. (2006) for details FA (Neutralization): please see publications. Flow (Cell Surface): 2-5 ug/ 1x10^6 cells IF/ICC: please see Schneeman et al (2005) for details. IP: please see Shimazu et al, 1999 for details Use in vitro assays reported in scientific literature (PMID 24676500) Use in Functional reported in scientific literature (PMID 25412776). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27744428). Use in Western blot reported in scientific literature (PMID 28546218).
Agonist
Antagonist
Publications
Read Publications using
NB100-56723 in the following applications:

Reactivity Notes

Cross reacts with Human and Dog.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR4 Antibody (HTA125)

  • ARMD10
  • CD284 antigen
  • CD284
  • hta125 tlr4
  • hta125
  • hTollhomolog of Drosophila toll
  • TLR4
  • TOLL
  • toll-like receptor 4

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TLR4 Antibody (NB100-56723)(50)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 10 applications: B/N, FLOW, Flow, Flow-CS, Func, ICC/IF, IHC-P, IP, In vitro, WB.


Filter By Application
B/N
(6)
FLOW
(8)
Flow
(3)
Flow-CS
(1)
Func
(1)
ICC/IF
(7)
IHC-P
(1)
IP
(1)
In vitro
(3)
WB
(2)
All Applications
Filter By Species
Human
(21)
Mouse
(1)
Canine
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using NB100-56723 Applications Species
Li F, Song R, Ao L et al. ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells Arterioscler thromb Vasc Biol 2017 May 25 [PMID: 28546218] (B/N, WB, Human)

Details:
This reference used the Azide Free version of NB100-56723.
B/N, WB Human
Heftrig D, Sturm R, Oppermann E et al. Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients. Mediators Inflamm. [PMID: 28255201]

Details:
Citation using the FITC form of this antibody.
Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J Allergy Clin Immunol. [PMID: 28219705] (Flow, Mouse)

Details:
Citation using the FITC form of this antibody.
Flow Mouse
Imai H, Fujita T, Kajiya M et al. Mobilization of TLR4 Into Lipid Rafts by Aggregatibacter Actinomycetemcomitans in Gingival Epithelial Cells. Cell. Physiol. Biochem. Nov 11 2016 [PMID: 27744428] (ICC/IF, Human) ICC/IF Human
Schmid W, Novacek G, Vogelsang H et al. Platelets' Toll-like receptor-4 in Crohn's disease. Eur. J. Clin. Invest. Oct 7 2016 [PMID: 27714784] (FLOW, Human) FLOW Human
Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine)

Details:
Used the PE form of this antibody.
FLOW Canine
Kopp F, Kupsch S, Schromm AB. Lipopolysaccharide-binding protein is bound and internalized by host cells and colocalizes with LPS in the cytoplasm: Implications for a role of LBP in intracellular LPS-signaling. Biochim. Biophys. Acta. 2016 Jan 22 [PMID: 26804480] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Perez-Figueroa E, Torres J, Sanchez-Zauco N et al. Activation of NLRP3 inflammasome in human neutrophils by Helicobacter pylori infection. Innate Immun. 2015 Nov 26 [PMID: 26610398] (B/N, In vitro, Human)

Details:
TLR4 antibody (clone HTA125) was used at 5 and 1ug/ml concentration for the treatment of human neutrophils for 1?hour at 37C which followed 24?hours treatment with Helicobacter pylori infection challenge.
B/N, In vitro Human
Jamin A, Dehoux L, Dossier C et al. Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome Clin Exp Immunol 2015 Dec [PMID: 26123900] (FLOW, Human)

Details:
This reference used the Azide Free version of NB100-56723.
FLOW Human
Jamin A, Dehoux L, Dossier C et al. Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome Clin. Clin. Exp. Immunol. 2015 Dec [PMID: 26123900] (FLOW, Human)

Details:
This reference used the Azide Free version of NB100-56723.
FLOW Human
Show All 50 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR4 Antibody (NB100-56723) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP2-24897
Biotin Labeled NBP2-24744
DyLight 550 Labeled NB100-56727R
DyLight 650 Labeled NB100-56727C
FITC Labeled NB100-56059
HRP Labeled NB100-56727H
PE Labeled NB600-728
PE Labeled NB100-56062

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Blogs on TLR4.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR4: Playing Roles in Apoptosis and Autoimmunity
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor (TLR) family that plays a key role in pathogen recognition and activation of innate immunity. Scientists have found that TLRs are highly conserved from Drosophila to humans, with a high...  Read full blog post.

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Bioinformatics

Gene Symbol TLR4
Entrez