bcl-x Antibody [Unconjugated]

Images

 
Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 104 cells, lane 1 and 2.5 x 104 cells, lane 2) and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of ...read more
Bcl-x was immunoprecipitated from lysates (7 x 105 cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x at approximately 34 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

bcl-x Antibody [Unconjugated] Summary

Immunogen
KLH-coupled Bcl-x synthetic peptide
CSAINGNPSWHLADSPAVNGA
Specificity
Detects human and mouse Bcl‑x in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 ug/mL
  • Simple Western 3 ug/mL
  • Immunoprecipitation 5 ug/10^6 cells
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
using
AF800 in the following applications:

Publications
Read Publications using
AF800 in the following applications:

  • IP
    1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for bcl-x Antibody [Unconjugated]

  • BCL2L1
  • Bclx
  • Bcl-x

Background

The peptide corresponds to amino acids 49-68 of human and mouse, long and short forms of Bcl-x. The sequence used for immunization differs significantly from the sequences of the corresponding region of other members of the Bcl-2 family. Cysteine was added to the amino-terminal for coupling to KLH and for coupling to an affinity matrix.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for bcl-x Antibody (AF800)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(5)
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Human
(4)
Mouse
(2)
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Showing Publications 1 - 6 of 6.
Publications using AF800 Applications Species
Schmitt E, Beauchemin M, Bertrand R Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint. Oncogene, 2007;26(40):5851-65. 2007 [PMID: 17369848] (IP,, Human) IP Human
Armstrong T, Murphy LB, Bateman AC, Conti JA, Fine DR, Johnson CD, Benyon RC, Iredale JP Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma. Clin. Cancer Res., 2004;10(21):7427-37. 2004 [PMID: 15534120] (WB,, Human) WB Human
Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer. Clin. Cancer Res., 2004;10(6):2100-8. 2004 [PMID: 15041731] (WB,, Human) WB Human
Raman VS, Akondy RS, Rath S, Bal V, George A Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses. J. Immunol., 2003;171(11):5876-81. 2003 [PMID: 14634097] (WB,, Mouse) WB Mouse
Sade H, Krishna S, Sarin A The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells. J. Biol. Chem., 2004;279(4):2937-44. 2004 [PMID: 14583609] (WB,, Human) WB Human
Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice. J. Exp. Med., 2002;196(1):87-95. 2002 [PMID: 12093873] (WB,, Mouse) WB Mouse

Review for bcl-x Antibody (AF800) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF800:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22582944
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22582944

Details

Detection NotesSee PMID 22582944

Comments

CommentsPublished: See PMID 22582944Independent review from antibodyresource.com

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Recent Reviews

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1
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Anonymous
01/09/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol BCL2L1