XIAP Antibody [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat ...read more
XIAP was detected in immersion fixed paraffin-embedded sections of human lymphoma using 5 µg/mL Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) overnight at 4 °C. Tissue ...read more
XIAP was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) overnight at ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for XIAP at approximately 59 kDa (as ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and XIAP knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat XIAP Antigen ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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XIAP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human XIAP
Met1-Ser497 (Ser162Cys)
Accession # P98170
Specificity
Detects human, mouse and rat XIAP in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
XIAP
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF8221 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XIAP Antibody [Unconjugated]

  • API3X-linked inhibitor of apoptosis protein
  • apoptosis inhibitor 3
  • baculoviral IAP repeat-containing 4
  • baculoviral IAP repeat-containing protein 4
  • BIRC4
  • BIRC4XLP2
  • E3 ubiquitin-protein ligase XIAP
  • EC 6.3.2.-
  • hIAP3
  • hIAP-3
  • hILP
  • IAP3
  • IAP-3
  • IAP-like protein
  • ILP
  • ILP1
  • Inhibitor of apoptosis protein 3
  • MIHA
  • XIAP
  • X-linked IAP
  • X-linked inhibitor of apoptosis

Background

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XIAP Antibody (AF8221)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Flow, IHC Fresh, IP, WB.


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Flow
(2)
IHC Fresh
(2)
IP
(1)
WB
(8)
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Filter By Species
Human
(10)
Mouse
(1)
Rat
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF8221 Applications Species
GA Ward, EJ Lewis, JS Ahn, CN Johnson, JF Lyons, V Martins, JM Munck, SJ Rich, T Smyth, NT Thompson, PA Williams, NE Wilsher, NG Wallis, G Chessari ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth Mol. Cancer Ther., 2018;0(0):. 2018 [PMID: 29695633] (IP,, Human) IP Human
R Fan, LM Schrott, T Arnold, S Snelling, M Rao, D Graham, A Cornelius, NL Korneeva Chronic oxycodone induces axonal degeneration in rat brain BMC Neurosci, 2018;19(1):15. 2018 [PMID: 29571287] (WB,, Rat) WB Rat
V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma Cancer Med, 2017;0(0):. 2017 [PMID: 28799237] (WB,, Human) WB Human
HL Hayes, BS Peterson, JM Haldeman, CB Newgard, HE Hohmeier, SB Stephens Delayed apoptosis allows islet ?-cells to implement an autophagic mechanism to promote cell survival PLoS ONE, 2017;12(2):e0172567. 2017 [PMID: 28212395] (WB,, Rat) WB Rat
Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies Molecules, 2016;21(8):. 2016 [PMID: 27483232] (Flow,, Human) Flow Human
Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study Onco Targets Ther, 2016;9(0):2601-13. 2016 [PMID: 27217778] (WB,, Human) WB Human
Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs). Anticancer Res, 2014;34(12):7007-20. 2014 [PMID: 25503127] (WB,, Human) WB Human
Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders. Int J Oncol, 2014;45(1):419-25. 2014 [PMID: 24789756] (Flow,, Human) Flow Human
Levin A, Iannuzzi M, Montgomery C, Trudeau S, Datta I, Adrianto I, Chitale D, McKeigue P, Rybicki B Admixture fine-mapping in African Americans implicates XAF1 as a possible sarcoidosis risk gene. PLoS ONE, 2014;9(3):e92646. 2014 [PMID: 24663488] (IHC Fresh,, Human) IHC Fresh Human
Toosi, Siavash, Habib, Nancy, Torres, Geneviev, Reynolds, Sandra R, Bystryn, Jean-Cla Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus. J Invest Dermatol, 2011;131(11):2327-9. 2011 [PMID: 21716321] (WB,, Human) WB Human
Show All 13 Publications.

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Bioinformatics Tool for XIAP Antibody (AF8221)

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Diseases for XIAP Antibody (AF8221)

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PTMs for XIAP Antibody (AF8221)

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Blogs on XIAP.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

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Bioinformatics

Gene Symbol XIAP
Entrez
Uniprot