The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor progression, fetal development, and cell migration. To further characterize and better understand apoptotic pathways, Xu’s group at MD Anderson used a survivin antibody in a DNA microarray screen upon promyelocytic leukemia protein (PML) target genes1. With their data, they identified a new PML4-dependent pathway that culminated in survivin as its main downstream target.
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Immunocytochemistry/Immunofluorescence: Survivin Antibody
Use of the Survivin antibody also enabled the establishment of a key role for the PI3-kinase/Akt/NF-kappaB/survivin signal in an HCAEC endothelial cell culturing system which took advantage of hypoxic preconditioning to enhance tubule formation and accelerated morphogenesis2. The survivin antibody was used for a comprehensive array of immunoblot, immunofluorescent, and immunohistochemical studies done in Lechler’s lab3. This group focused on characterizing survivin expression in the pathogenesis and marked chemoresistance found in particular subsets of high grade human chondrosarcomas. The role of survivin in another cancer type – that of breast cancer – was also investigated with the survivin antibody in the Ryan lab, who analyzed various survivin splice isoforms (2B, 3B, and DeltaEx3) in normal tissue, adenomas, and primary carcinomas4. Further oncology immunohistochemical studies were carried out with the survivin antibody in colon samples, where the resulting different subcellular localization patterns for wild-type and mutant forms of the adenomatous polyposis coli protein APC tumor suppressor provided interesting insights into how particular APC mutations govern tumor cell survival5.
Novus Biologicals offers survivin reagents for your research needs including:
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