SMAC/Diablo Antibody


Western Blot: SMAC/Diablo Antibody [NB100-56311] - Analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.
Immunohistochemistry: SMAC/Diablo Antibody [NB100-56311] - Analysis of human prostate using Smac antibody at 10 ug/ml.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
0.5 mg/ml

Order Details

SMAC/Diablo Antibody Summary

This rabbit polyclonal antibody was developed against two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac (Genbank accession no. AAF87716).
Protein G purified
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  • Immunocytochemistry/Immunofluorescence 1:10-1:500. Use reported in scientific literature (Hu et al (2004))
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-2 ug/ml
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (Ren et al. (2006))
HeLa Whole Cell Lysate
Read Publications using
NB100-56311 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22751125).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
0.5 mg/ml
Protein G purified

Alternate Names for SMAC/Diablo Antibody

  • 0610041G12Rik
  • Diablo
  • diablo, IAP-binding mitochondrial protein
  • Direct IAP-binding protein with low pI
  • mitochondrial Smac protein
  • Second mitochondria-derived activator of caspase
  • SMAC
  • SMACmitochondrial


Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239 amino acids, predicting a protein with a molecular weight of 27 kDa. However, the mature protein purified from HeLa cells is only about 25 kDa.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: IHC, IHC-P, IP, WB
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Applications: ICC, Simple Western, WB
Species: Ma, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Species: Hu, Mu
Applications: IP, Simple Western, WB
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
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Applications: CyTOF-ready, Flow-CS, Flow, ICC, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: ELISA, WB
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
Species: Hu, Mu
Applications: WB, ICC/IF, IHC
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Publications for SMAC/Diablo Antibody (NB100-56311)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-56311 Applications Species
Qiu W, Liu H, Sebastini A et al. An apoptosis-independent role of SMAC in tumor suppression. Oncogene. 2013-05-09 [PMID: 22751125] (IHC-P, WB, Mouse)

IMGENEX Product Cited: SMAC (IMG-248A). 1. IHC(paraffin): Human colon tumors, Fig 1A 2. WB: Mouse colonic mucosa from WT and SMACK knockout mice, Figs 2D. 3. WB: Mouse small intestinal mucosa from WT and SMACK knockout mice, Fig 6A 4. WB: HCT116 cell line
IHC-P, WB Mouse
Endo Kazuya, Kohnoe Shunji, Watanabe Akihiro et al. Clinical significance of Smac/DIABLO expression in colorectal cancer. Oncol Rep. 2009-02-01 [PMID: 19148507] (Human) Human
Conus S, Perozzo R, Reinheckel T et al. Caspase-8 is activated by cathepsin D initiating neutrophil apoptosis during the resolution of inflammation. J Exp Med. 2008-03-17 [PMID: 18299403]

IF (confocal microscopy on human neutrophils), Fig. 2B.
Ren Y, Akyurek N, Schlette E et al. Expression of Smac/DIABLO in B-cell non-Hodgkin and Hodgkin lymphomas. Hum Pathol. 2006-11-01 [PMID: 16949641] (IHC-P, WB) IHC-P, WB
Liu T, Biddle D, Hanks AN et al. Activation of dual apoptotic pathways in human melanocytes and protection by survivin. J Invest Dermatol. 2006-10-01 [PMID: 16728972] (WB, Human)

Smac/DIABLO (IMG-248A). WB: Mitochondrial and cytosolic fractions of human melanocytes treated with either UV radiation (UVB), 4-tert-butylphenol (4-TBP), or cisplatin (CP), Fig 4A.
WB Human
Qin JZ, Ziffra J, Stennett L et al. Proteasome inhibitors trigger NOXA-mediated apoptosis in melanoma and myeloma cells. Cancer Res. 2005-07-15 [PMID: 16024630]
Berthier A, Lemaire-Ewing S, Prunet C et al. 7-Ketocholesterol-induced apoptosis. Involvement of several pro-apoptotic but also anti-apoptotic calcium-dependent transduction pathways. FEBS J. 2005-06-01 [PMID: 15955068] (WB)

Smac/DIABLO (IMG-248A). WB: THP-1 cells treated with 7-ketocholestrol, Fig 4.
Lee JC, Lee CH, Su CL et al. Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells. Carcinogenesis. 2005-10-01 [PMID: 15905197] (WB)

Caspase-3 (IMG-144A) [WB, Fig 3A (HT-29 and HCT 116 cells)].
Mizutani Y, Nakanishi H, Yamamoto K et al. Downregulation of Smac/DIABLO expression in renal cell carcinoma and its prognostic significance. J Clin Oncol. 2005-01-20 [PMID: 15572731] (WB, Human)

Smac/DIABLO (IMG-248). WB: Human renal cell carcinoma (RCC) normal kidney, Fig 1 & 2.
WB Human
Hu P, Han Z, Couvillon AD, Exton JH. Critical role of endogenous Akt/IAPs and MEK1/ERK pathways in counteracting endoplasmic reticulum stress-induced cell death. J Biol Chem. 2004-11-19 [PMID: 15339911] (ICC/IF)

IF/ICC: MCF-7 cells treated with thapsigargin, Fig 7A.
Show All 16 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies


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Blogs on SMAC/Diablo.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were ...  Read full blog post.

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Gene Symbol DIABLO