SMAC/Diablo Antibody

Images

 
Western Blot: SMAC/Diablo Antibody [NB100-56311] - Analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.
Immunohistochemistry: SMAC/Diablo Antibody [NB100-56311] - Analysis of human prostate using Smac antibody at 10 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SMAC/Diablo Antibody Summary

Immunogen
This rabbit polyclonal antibody was developed against two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac (Genbank accession no. AAF87716).
Specificity
Smac
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIABLO
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Immunocytochemistry/Immunofluorescence: See Hu et al (2004) for details. Immunohistochemistry-Paraffin:10 ug/ml please see Ren et al. for details (2006).
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB100-56311 in the following applications:

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for SMAC/Diablo Antibody

  • 0610041G12Rik
  • Diablo
  • diablo, IAP-binding mitochondrial protein
  • Direct IAP-binding protein with low pI
  • mitochondrial Smac protein
  • Second mitochondria-derived activator of caspase
  • SMAC
  • SMACmitochondrial

Background

Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239 amino acids, predicting a protein with a molecular weight of 27 kDa. However, the mature protein purified from HeLa cells is only about 25 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMAC/Diablo Antibody (NB100-56311)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(2)
WB
(8)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-56311 Applications Species
Qiu W, Liu H, Sebastini A et al. An apoptosis-independent role of SMAC in tumor suppression. Oncogene. 2013 May 9 [PMID: 22751125] (IHC-P, WB,, Mouse)

Details:
IMGENEX Product Cited: SMAC (IMG-248A). 1. IHC(paraffin): Human colon tumors, Fig 1A 2. WB: Mouse colonic mucosa from WT and SMACK knockout mice, Figs 2D. 3. WB: Mouse small intestinal mucosa from WT and SMACK knockout mice, Fig 6A 4. WB: HCT116 cell line
IHC-P, WB Mouse
Endo Kazuya, Kohnoe Shunji, Watanabe Akihiro et al. Clinical significance of Smac/DIABLO expression in colorectal cancer. Oncol Rep. 2009 Feb [PMID: 19148507] (Human) Human
Conus S, Perozzo R, Reinheckel T et al. Caspase-8 is activated by cathepsin D initiating neutrophil apoptosis during the resolution of inflammation. J Exp Med. 2008 Mar 17 [PMID: 18299403]

Details:
IF (confocal microscopy on human neutrophils), Fig. 2B.
Ren Y, Akyurek N, Schlette E et al. Expression of Smac/DIABLO in B-cell non-Hodgkin and Hodgkin lymphomas. Hum Pathol. 2006 Nov [PMID: 16949641] (WB, IHC-P) WB, IHC-P
Liu T, Biddle D, Hanks AN et al. Activation of dual apoptotic pathways in human melanocytes and protection by survivin. J Invest Dermatol. 2006 Oct [PMID: 16728972] (WB, Human)

Details:
Smac/DIABLO (IMG-248A). WB: Mitochondrial and cytosolic fractions of human melanocytes treated with either UV radiation (UVB), 4-tert-butylphenol (4-TBP), or cisplatin (CP), Fig 4A.
WB Human
Qin JZ, Ziffra J, Stennett L et al. Proteasome inhibitors trigger NOXA-mediated apoptosis in melanoma and myeloma cells. Cancer Res. 2005 Jul 15 [PMID: 16024630]
Berthier A, Lemaire-Ewing S, Prunet C et al. 7-Ketocholesterol-induced apoptosis. Involvement of several pro-apoptotic but also anti-apoptotic calcium-dependent transduction pathways. FEBS J. 2005 Jun [PMID: 15955068] (WB)

Details:
Smac/DIABLO (IMG-248A). WB: THP-1 cells treated with 7-ketocholestrol, Fig 4.
WB
Lee JC, Lee CH, Su CL et al. Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells. Carcinogenesis. 2005 Oct [PMID: 15905197] (WB)

Details:
Smac/DIABLO (IMG-248A). WB: HT-29 and HCT-116 cells treated with justicidin A for different time intervals, Fig 5.
WB
Mizutani Y, Nakanishi H, Yamamoto K et al. Downregulation of Smac/DIABLO expression in renal cell carcinoma and its prognostic significance. J Clin Oncol. 2005 Jan 20 [PMID: 15572731] (WB, Human)

Details:
Smac/DIABLO (IMG-248). WB: Human renal cell carcinoma (RCC) normal kidney, Fig 1 & 2.
WB Human
Hu P, Han Z, Couvillon AD, Exton JH. Critical role of endogenous Akt/IAPs and MEK1/ERK pathways in counteracting endoplasmic reticulum stress-induced cell death. J Biol Chem. 2004 Nov 19 [PMID: 15339911] (ICC/IF)

Details:
IF/ICC: MCF-7 cells treated with thapsigargin, Fig 7A.
ICC/IF
Show All 16 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

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Blogs on SMAC/Diablo.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were...  Read full blog post.

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Bioinformatics

Gene Symbol DIABLO
Entrez
Uniprot