Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.

Flow Cytometry: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple).

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 3B10E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Alkaline Phosphatase, Intestinal (AA: 397-458) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALPI
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 56.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free

• alkaline phosphatase, intestinal
• Alkaline phosphomonoesterase
• ALPI
• EC 3.1.3.1
• Glycerophosphatase
• IAP
• Intestinal alkaline phosphatase
• intestinal-type alkaline phosphatase
• Kasahara isozyme

Background

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALPI