CD47 Antibody (B6H12.2) - Azide and BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The ...read more
Western Blot: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Detection of CD47 expression on two hematological cancer cell lines, Jurkat and Raji. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human breast cancer tissue stained with CD47 Antibody (B6H12.2). IHC-P image submitted by a verified customer review.
Flow Cytometry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on A431 cells with CD47 Antibody [B6H12.2] NBP2-31106PCP (blue) and a matched isotype control (orange). Cells were ...read more
Western Blot: CD47 Antibody (B6H12.2) [Azide Free] [NBP2-31106] - Human brain (lane 1) and testis (lane 2) protein was separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and then ...read more
Immunocytochemistry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human trabecular meshwork (primary ocular cells). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The ...read more
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar ...read more
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar ...read more
Flow (Cell Surface): CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on human peripheral blood lymphocytes with CD47 (B6H12.2) antibody NBP2-31106 (blue) and a matched isotype control ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-CS, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, ICC/IF
Clone
B6H12.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

CD47 Antibody (B6H12.2) - Azide and BSA Free Summary

Immunogen
This antibody was raised against CD47.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD47
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 1 ug/mL
  • Immunocytochemistry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 ug/mL
  • Immunoprecipitation
  • Western Blot 1:1000
Application Notes
This antibody is CyTOF ready.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP2-31106 in the following applications:

Publications
Read Publications using
NBP2-31106 in the following applications:

  • 1 publication
  • 2 publications
  • IP
    2 publications
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30833751).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD47 Antibody (B6H12.2) - Azide and BSA Free

  • antigen identified by monoclonal 1D8
  • Antigenic surface determinant protein OA3
  • CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
  • CD47 antigen
  • CD47 glycoprotein
  • CD47 molecule
  • CD47
  • IAP
  • IAPintegrin associated protein
  • Integrin-associated protein
  • leukocyte surface antigen CD47
  • MER6
  • MER6integrin-associated signal transducer
  • OA3
  • Protein MER6
  • Rh-related antigen

Background

CD47, originally named integrin-associated protein (IAP), is a 50-kDa protein containing five membrane spanning sequences and a short cytoplasmic tail. CD47 appears to be important in myeloid cell activation and migration across endothelial and epithelial monolayers. Anti-CD47 antibody has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47 and its ligands, thrombospondin 1 (TSP-1) and SIRP-, are important regulators of dendritic cells. Recently, it has been shown that CD47 and TLR2 cross-talk to regulate PMN transmigration. The activation of TLR2/6 by MALP-2, a TLR2 specific agonist, potently inhibits human and murine PMN transmigration. The cross-talk between TLR2 and CD47 may be mediated through MYD88.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for CD47 Antibody (NBP2-31106)(4)

Reviews for CD47 Antibody (NBP2-31106) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP2-31106:
Filter by Applications
IHC-P
(1)
WB
(1)
ICC
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD47 NBP2-31106
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5
reviewed by:
Michael Nowak
IHC-P Human 11/04/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast cancer tissue
SpeciesHuman
LotB6H12.2

Comments

Comments4% formaldehyde fixed paraffin sections.
Western Blot CD47 NBP2-31106
Enlarge
5
reviewed by:
PRABIR CHAKRABORTY
WB Human 05/19/2019
View

Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
SpeciesHuman
LotAB10610A-06

Comments

CommentsSamples were prepared in 4X lamelli buffer and were not boiled. Boiling causes loss of signal in WB.
Immunocytochemistry CD47 NBP2-31106
Enlarge
4
reviewed by:
MIN KANG
ICC Human 04/25/2018
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman trabecular meshwork
SpeciesHuman
LotAB010610A-05

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD47 Antibody (NBP2-31106) (0)

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Secondary Antibodies

 

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Michael Nowak
11/04/2021
Application: IHC-P
Species: Human

PRABIR CHAKRABORTY
05/19/2019
Application: WB
Species: Human

MIN KANG
04/25/2018
Application: ICC
Species: Human

Bioinformatics

Gene Symbol CD47
OMIM