CD47 Antibody (B6H12.2) - Azide Free

Images

 
Western Blot: CD47 Antibody (B6H12.2) [Azide Free] [NBP2-31106] - Human brain (lane 1) and testis (lane 2) protein was separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and then ...read more
Immunocytochemistry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human trabecular meshwork (primary ocular cells). Image from verified customer review.
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar ...read more
Flow (Cell Surface): CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on human peripheral blood lymphocytes with CD47 (B6H12.2) antibody NBP2-31106 (blue) and a matched isotype control ...read more
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-CS, IHC, IHC-P, IP, CyTOF-ready, ICC/IF
Clone
B6H12.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide Free
Concentration
1.0 mg/ml

CD47 Antibody (B6H12.2) - Azide Free Summary

Immunogen
This antibody was raised against CD47
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD47
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5ug/ml
  • Immunoprecipitation
  • CyTOF-ready
  • Flow (Cell Surface)
  • Immunocytochemistry
Application Notes
This antibody is Cytof ready.
Control
CD47 Lysate (NBP2-65671)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-31106 in the following applications:

Publications
Read Publications using
NBP2-31106 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD47 Antibody (B6H12.2) - Azide Free

  • antigen identified by monoclonal 1D8
  • Antigenic surface determinant protein OA3
  • CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
  • CD47 antigen
  • CD47 glycoprotein
  • CD47 molecule
  • CD47
  • IAP
  • IAPintegrin associated protein
  • Integrin-associated protein
  • leukocyte surface antigen CD47
  • MER6integrin-associated signal transducer
  • OA3
  • Protein MER6
  • Rh-related antigen

Background

CD47, originally named integrin-associated protein (IAP), is a 50-kDa protein containing five membrane spanning sequences and a short cytoplasmic tail. CD47 appears to be important in myeloid cell activation and migration across endothelial and epithelial monolayers. Anti-CD47 antibody has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47 and its ligands, thrombospondin 1 (TSP-1) and SIRP-, are important regulators of dendritic cells. Recently, it has been shown that CD47 and TLR2 cross-talk to regulate PMN transmigration. The activation of TLR2/6 by MALP-2, a TLR2 specific agonist, potently inhibits human and murine PMN transmigration. The cross-talk between TLR2 and CD47 may be mediated through MYD88.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD47 Antibody (NBP2-31106)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Filter By Species
Human
(1)
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Review for CD47 Antibody (NBP2-31106) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-31106:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry CD47 NBP2-31106
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reviewed by:
Anonymous
ICC Human 04/25/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman trabecular meshwork
SpeciesHuman
LotAB010610A-05

Product General Protocols

View specific protocols for CD47 Antibody (NBP2-31106): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD47 Antibody (NBP2-31106) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD47 Antibody (NBP2-31106)

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Diseases for CD47 Antibody (NBP2-31106)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
04/25/2018
Application: ICC
Species: Human

Bioinformatics

Gene Symbol CD47
OMIM