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CD47 Antibody (B6H12.2) - Azide Free

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Western Blot: CD47 Antibody (B6H12.2) [Azide Free] [NBP2-31106] - Human brain (lane 1) and testis (lane 2) protein was separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and then probed with 2 ug/ml of anti-CD47. CD47 protein was detected using an anti-mouse HRP secondary antibody.
Western Blot: CD47 Antibody (B6H12.2) [Azide Free] [NBP2-31106] - Human brain (lane 1) and testis (lane 2) protein was separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and then ...read more
Immunocytochemistry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human trabecular meshwork (primary ocular cells). Image from verified customer review.
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar ...read more
Flow (Cell Surface): CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on human peripheral blood lymphocytes with CD47 (B6H12.2) antibody NBP2-31106 (blue) and a matched isotype control ...read more
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-CS, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, ICC/IF
Clone
B6H12.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-31106AF350
Alexa Fluor 488
NBP2-31106AF488
Alexa Fluor 532
NBP2-31106AF532
Alexa Fluor 594
NBP2-31106AF594
Alexa Fluor 647
NBP2-31106AF647
Alexa Fluor 750
NBP2-31106AF750
Allophycocyanin
NBP2-31106APC
Allophycocyanin/Cy7
NBP2-31106APCCY7
Biotin
NBP2-31106B
DyLight 350
NBP2-31106UV
DyLight 405
NBP2-31106V
DyLight 488
NBP2-31106G
DyLight 550
NBP2-31106R
DyLight 594
NBP2-31106DL594
DyLight 650
NBP2-31106C
DyLight 680
NBP2-31106FR
DyLight 755
NBP2-31106IR
FITC
NBP2-31106F
HRP
NBP2-31106H
Janelia Fluor 549
NBP2-31106JF549
Janelia Fluor 646
NBP2-31106JF646
PE
NBP2-31106PE
PE/Atto594
NBP2-31106PEATT594
PE/Cy5.5
NBP2-31106PECY55
PE/Cy7
NBP2-31106PECY7
PerCP
NBP2-31106PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide Free
NBP2-31106-25ug
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CD47 Antibody (B6H12.2) - Azide Free Summary

Immunogen
This antibody was raised against CD47
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD47
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1 ug/mL
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5ug/ml
  • Immunoprecipitation
  • CyTOF-ready
  • Flow (Cell Surface)
  • Immunocytochemistry
Application Notes
This antibody is Cytof ready. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30203645).
Control
CD47 Lysate (NBP2-65671)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-31106 in the following applications:
Publications
Read Publications using
NBP2-31106 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD47 Antibody (B6H12.2) - Azide Free

  • antigen identified by monoclonal 1D8
  • Antigenic surface determinant protein OA3
  • CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
  • CD47 antigen
  • CD47 glycoprotein
  • CD47 molecule
  • CD47
  • IAP
  • IAPintegrin associated protein
  • Integrin-associated protein
  • leukocyte surface antigen CD47
  • MER6integrin-associated signal transducer
  • OA3
  • Protein MER6
  • Rh-related antigen

Background

CD47, originally named integrin-associated protein (IAP), is a 50-kDa protein containing five membrane spanning sequences and a short cytoplasmic tail. CD47 appears to be important in myeloid cell activation and migration across endothelial and epithelial monolayers. Anti-CD47 antibody has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47 and its ligands, thrombospondin 1 (TSP-1) and SIRP-, are important regulators of dendritic cells. Recently, it has been shown that CD47 and TLR2 cross-talk to regulate PMN transmigration. The activation of TLR2/6 by MALP-2, a TLR2 specific agonist, potently inhibits human and murine PMN transmigration. The cross-talk between TLR2 and CD47 may be mediated through MYD88.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for XIAP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for XIAP at approximately 59 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat XIAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8221) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Immunocytochemistry/Immunofluorescence: Survivin Antibody [NB500-201] - Analysis using the HRP conjugate of NB500-201. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using NB500-201.
Western Blot: Survivin Antibody [NB500-201] - Analysis of 30ug of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal Survivin antibody (NB 500-201) at 1ug/ml. Detection was performed using ECL method with 1 minute exposure.
Immunocytochemistry/Immunofluorescence: Survivin Antibody [NB500-201] - Analysis of HeLa cells using Survivin antibody (NB500-201, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Immunohistochemistry: SMAC/Diablo Antibody [NB100-56311] - Analysis of human prostate using Smac antibody at 10 ug/ml.
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NB500-201
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[Unconjugated]
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Publications for CD47 Antibody (NBP2-31106)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Human
(1)
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Review for CD47 Antibody (NBP2-31106) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-31106:
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ICC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry CD47 NBP2-31106
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reviewed by:
Anonymous
 ICC Human 04/25/2018
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman trabecular meshwork
SpeciesHuman
LotAB010610A-05

Product General Protocols

View specific protocols for CD47 Antibody (NBP2-31106): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD47 Antibody (NBP2-31106) (0)

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Control Lysate(s)

CD47 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Immunocytochemistry CD47 NBP2-31106
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 Anonymous
04/25/2018
Application: ICC
Species: Human
read this review

Bioinformatics

Gene Symbol CD47
OMIM