![Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] (NBP2-31106) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/CD47-Antibody-(B6H12.2)---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-31106-img0012.jpg "Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] (NBP2-31106) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, Flow-CS, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, ICC/IF |
| Clone | B6H12.2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | This antibody was raised against CD47. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD47 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody is CyTOF ready. |
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| Reviewed Applications |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0014.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0015.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Protein Array: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA DQ/DR/DP Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Protein-Array-NBP2-79843-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79843-img0002.jpg)
![Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Western-Blot-NBP2-79843-img0003.jpg)
![Flow Cytometry: Survivin Antibody [NB500-201] - An intracellular stain was performed on HeLa cells with [NB500-201] and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/images2/Survivin-Antibody-Flow-Cytometry-NB500-201-img0025.jpg)
![Dual RNAscope ISH-IHC: Survivin Antibody [NB500-201] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells.](http://images.novusbio.com/images2/Survivin-Antibody-Dual-RNAscope-ISH-IHC-NB500-201-img0026.jpg)
![Knockdown Validated: Survivin Antibody [NB500-201] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in the tumor tissues on day 19 using [NB500-201]. Scale bar, 50 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0116022) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Survivin-Antibody-Knockdown-Validated-NB500-201-img0033.jpg)
![Immunocytochemistry/Immunofluorescence: cIAP-2/HIAP-1 Antibody [NBP1-27972] - HIAD1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/images2/cIAP-2-HIAP-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-27972-img0012.jpg)
![Simple Western: cIAP-2/HIAP-1 Antibody [NBP1-27972] - Simple Western lane view shows a specific band for Ciap2 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/images2/cIAP-2-HIAP-1-Antibody-Simple-Western-NBP1-27972-img0011.jpg)
![Immunohistochemistry: cIAP-2/HIAP-1 Antibody [NBP1-27972] - Analysis of human adenocarcinoma cells and adjacent glands using anti-cIAP2.](http://images.novusbio.com/images2/cIAP-2-HIAP-1-Antibody-Immunohistochemistry-NBP1-27972-img0002.jpg)
![Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Exacerbated apoptosis and altered inflammation in double-deficient Atg7deltapan-Rip3-/- mice. (c) Depletion of Rip3 and pancreatic Atg7 (Atg7deltapan-Rip3d/d) increased pancreatic caspase-9 in 12-week-old mice. Caspase-9 quantitation and representative IF microphotographs of Atg7deltapan (n=5) and Atg7deltapan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (red) (1/1000).. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0012.jpg)
![Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Increased the expression of Caspase-9 in 12-week-old Atg7deltapan mice. Caspase-9 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (green) (1/1000, scale bar=50?um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0013.jpg)
![Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Mice with two different spontaneous mutant alleles of Sharpin show variations in onset of CPDM phenotype. However, the onset is much more rapid in Sharpincpdm-Dem mice as evidenced by antibody detection of cleaved CASPASE 9 (T) when compared to Sharpincpdm mutants (R) or WT mice (Q,S). . Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085666) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0014.jpg)
![Flow Cytometry: L1CAM Antibody (UJ127.11) [NB100-2682] - A surface stain was performed on HeLa cells with L1CAM Antibody (UJ127.11) NB100-2682 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/images2/L1CAM-Antibody-(UJ127.11)-Flow-Cytometry-NB100-2682-img0001.jpg)
![Western Blot: L1CAM Antibody (UJ127.11) [NB100-2682] - Total protein from human HeLa, A431 and SHSY-5Y cell lines and human Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-L1CAM (NB100-2682) in blocking buffer and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/images2/L1CAM-Antibody-(UJ127.11)-Western-Blot-NB100-2682-img0002.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (UJ127.11) [NB100-2682] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of L1CAM [UJ127.11] antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and membrane staining as observed in the cerebral cortex](http://images.novusbio.com/images2/L1CAM-Antibody-(UJ127.11)-Immunohistochemistry-Paraffin-NB100-2682-img0003.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Michael Nowak |
IHC-P | Human | 11/04/2021 |
Summary
Comments
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Enlarge |
reviewed by:
PRABIR CHAKRABORTY |
WB | Human | 05/19/2019 |
Summary
Comments
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Enlarge |
reviewed by:
MIN KANG |
ICC | Human | 04/25/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for CD47 Antibody (NBP2-31106)Discover more about diseases related to CD47 Antibody (NBP2-31106).
| Pathways for CD47 Antibody (NBP2-31106)View related products by pathway.
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PTMs for CD47 Antibody (NBP2-31106)Learn more about PTMs related to CD47 Antibody (NBP2-31106).
| Research Areas for CD47 Antibody (NBP2-31106)Find related products by research area.
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| Michael Nowak 11/04/2021 |
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| Application: | IHC-P | |
| Species: | Human |
| PRABIR CHAKRABORTY 05/19/2019 |
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| Application: | WB | |
| Species: | Human |
| MIN KANG 04/25/2018 |
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| Application: | ICC | |
| Species: | Human |
![Western Blot: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Detection of CD47 expression on two hematological cancer cell lines, Jurkat and Raji. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/CD47-Antibody-(B6H12.2)---Azide-Free-Western-Blot-NBP2-31106-img0008.jpg "Western Blot: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Detection of CD47 expression on two hematological cancer cell lines, Jurkat and Raji. WB image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human breast cancer tissue stained with CD47 Antibody (B6H12.2). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/CD47-Antibody-(B6H12.2)---Azide-Free-Immunohistochemistry-Paraffin-NBP2-31106-img0010.jpg "Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human breast cancer tissue stained with CD47 Antibody (B6H12.2). IHC-P image submitted by a verified customer review.")
![Flow Cytometry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on A431 cells with CD47 Antibody [B6H12.2] NBP2-31106PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize2/CD47-Antibody-(B6H12.2)---Azide-Free-Flow-Cytometry-NBP2-31106-img0009.jpg "Flow Cytometry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on A431 cells with CD47 Antibody [B6H12.2] NBP2-31106PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.")
![Western Blot: CD47 Antibody (B6H12.2) [Azide Free] [NBP2-31106] - Human brain (lane 1) and testis (lane 2) protein was separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and then probed with 2 ug/ml of anti-CD47. CD47 protein was detected using an anti-mouse HRP secondary antibody.](http://images.novusbio.com/fullsize2/CD47-Antibody-(B6H12.2)-[Azide-Free]-Western-Blot-NBP2-31106-img0004.jpg "Western Blot: CD47 Antibody (B6H12.2) [Azide Free] [NBP2-31106] - Human brain (lane 1) and testis (lane 2) protein was separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and then probed with 2 ug/ml of anti-CD47. CD47 protein was detected using an anti-mouse HRP secondary antibody.")
![Immunocytochemistry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human trabecular meshwork (primary ocular cells). Image from verified customer review.](http://images.novusbio.com/fullsize2/CD47-Antibody-(B6H12.2)---Azide-Free-Immunocytochemistry-NBP2-31106-img0007.jpg "Immunocytochemistry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human trabecular meshwork (primary ocular cells). Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] conjugated to Biotin (NBP2-31106B) at 5 ug/ml for 1 hour at room temperature then detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/CD47-Antibody-(B6H12.2)---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-31106-img0011.jpg "Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] conjugated to Biotin (NBP2-31106B) at 5 ug/ml for 1 hour at room temperature then detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar epithelial cells in the breast section developed specific membrane-cytoplasmic staining.](http://images.novusbio.com/fullsize2/CD47-Antibody-(B6H12.2)-[Azide-Free]-Immunohistochemistry-Paraffin-NBP2-31106-img0003.jpg "Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar epithelial cells in the breast section developed specific membrane-cytoplasmic staining.")
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar epithelial cells in the breast section developed specific membrane-cytoplasmic staining.](http://images.novusbio.com/fullsize2/CD47-Antibody-(B6H12.2)-[Azide-Free]-Immunohistochemistry-Paraffin-NBP2-31106-img0002.jpg "Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar epithelial cells in the breast section developed specific membrane-cytoplasmic staining.")
![Flow (Cell Surface): CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on human peripheral blood lymphocytes with CD47 (B6H12.2) antibody NBP2-31106 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/fullsize2/CD47-Antibody-(B6H12.2)---Azide-Free-Flow-(Cell-Surface)-NBP2-31106-img0006.jpg "Flow (Cell Surface): CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on human peripheral blood lymphocytes with CD47 (B6H12.2) antibody NBP2-31106 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].")
![Western Blot: SMAC/Diablo Antibody [NB100-56311] - Analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.](http://images.novusbio.com/images2/SMAC-Diablo-Antibody-Western-Blot-NB100-56311-img0006.jpg)
![Immunohistochemistry: SMAC/Diablo Antibody [NB100-56311] - Analysis of human prostate using Smac antibody at 10 ug/ml.](http://images.novusbio.com/images2/SMAC-Diablo-Antibody-Immunohistochemistry-NB100-56311-img0007.jpg)
![Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Low power](http://images.novusbio.com/images2/SIRP-alpha-CD172a-Antibody-(OX-41)-Immunofluorescence-NB100-65530-img0001.jpg)
![Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Medium power](http://images.novusbio.com/images2/SIRP-alpha-CD172a-Antibody-(OX-41)-Immunofluorescence-NB100-65530-img0002.jpg)
![Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power](http://images.novusbio.com/images2/SIRP-alpha-CD172a-Antibody-(OX-41)-Immunofluorescence-NB100-65530-img0003.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03] - Analysis of ALPI expression in HeLa (Cat # L013V1).](http://images.novusbio.com/images2/Alkaline-Phosphatase--Intestinal-Antibody-(3A8)-Western-Blot-H00000248-M03-img0003.jpg)
![ELISA: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03] - Detection limit for recombinant GST tagged ALPI is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/images2/Alkaline-Phosphatase--Intestinal-Antibody-(3A8)-ELISA-H00000248-M03-img0002.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - EA reduced MPP+-induced dopaminergic neuronal apoptosis by increasing BDNF (brain-derived neurotrophic factor) expression and further Akt phosphorylation in the rat substantia nigra. Eight days after MPP+ administration, our Western blot results (MAB7566) show that MPP+ treatment reduced tyrosine hydroxylase and Bcl-2 expression in the ipsilateral side of the rat substantia nigra (SN), but not in the contralateral side. EA stimulation (50 Hz) enhanced mature BDNF, tyrosine hydroxylase, and Bcl-2 expression in the MPP+-treated ipsilateral side. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/18/9/1846), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Bcl-2-Antibody-Western-Blot-NB100-56098-img0012.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.](http://images.novusbio.com/images2/Bcl-2-Antibody-Western-Blot-NB100-56098-img0004.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 using this antibody at 1:2000, 20 ug/protein was loaded per lane. (1) Human Jurkat T cells. (2) Human RS11 lymphoma cells. (3-6) Human breast cancer cases. Patient sample in lane 5 lacked detectible Bcl-2 expression.](http://images.novusbio.com/images2/Bcl-2-Antibody-Western-Blot-NB100-56098-img0005.jpg)
![Western Blot: Livin Antibody (88C570) [NB100-56548] - detection of Livin in A) Livin transfected cell lysate and B) Molt4 cell lysate. A protein band of apprximate molecular weight of 31 kDa is detected using this antibody.](http://images.novusbio.com/images2/Livin-Antibody-(88C570)-Western-Blot-NB100-56548-img0002.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-(MG1)-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
