Recombinant Human TRAIL/TNFSF10 Protein


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Recombinant Human TRAIL/TNFSF10 Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-12 ng/mL.
The apoptotic effect of soluble trimeric rhTRAIL can be enhanced by oligimerization of the soluble rhTRAIL through the use of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). The ED50 for this effect is 1-4 ng/mL.
Note: This is one of multiple forms available for this protein. Check R&D Systems' website,, for a complete listing of the variants.
Mouse myeloma cell line, NS0-derived human TRAIL/TNFSF10 protein
Thr95-Gly281, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Structure / Form
Noncovalently-linked homotrimer and disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
21 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
24 kDa, reducing conditions
Reviewed Applications
Read 1 Review rated 5
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375-TL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 20 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TRAIL/TNFSF10 Protein

  • Apo-2 ligand
  • APO2L
  • Apo-2Ltumor necrosis factor (ligand) family, member 10
  • APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
  • CD253 antigen
  • CD253
  • Protein TRAIL
  • TL2
  • TNF-related apoptosis-inducing ligand
  • TNFSF10
  • TRAILTNF-related apoptosis inducing ligand TRAIL
  • tumor necrosis factor (ligand) superfamily, member 10
  • tumor necrosis factor ligand superfamily member 10


TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the new TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is most homologous to FAS ligand, sharing 28% amino acid sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that tranduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified (1 - 3). Osteoprotegerin has been identified as a fifth TRAIL receptor (4).

  1. Golstein, P. (1997) Current Biology 7:R750.
  2. Wiley, S.R. et al. (1995) Immunity 3:673.
  3. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
  4. Emery, J. et al. (1998) J. Biol. Chem. 273:14363.

Publications for TRAIL/TNFSF10 (375-TL)(54)

We have publications tested in 5 confirmed species: Human, Mouse, Complex Species Category, Porcine, Xenograft.

We have publications tested in 4 applications: Bioassay, Cell Culture, In Vivo, Surface Plasmon Resonance (SPR.

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Showing Publications 1 - 10 of 54. Show All 54 Publications.
Publications using 375-TL Applications Species
Exconde, PM;Hernandez-Chavez, C;Bourne, CM;Richards, RM;Bray, MB;Lopez, JL;Srivastava, T;Egan, MS;Zhang, J;Yoo, W;Shin, S;Discher, BM;Taabazuing, CY; The tetrapeptide sequence of IL-18 and IL-1? regulates their recruitment and activation by inflammatory caspases Cell reports 2023-12-15 [PMID: 38103201] (Bioassay, Human) Bioassay Human
Bravo Perina, L;Faria Gomes, IN;Alcantara Pelloso, AR;Silva, VAO;Rebolho Batista Arantes, LM;Eliseo Melendez, M; Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines Scientific reports 2023-10-21 [PMID: 37865660] (Bioassay, Human) Bioassay Human
Y Shlyakhtin, B Bloechl, MM Portal BdLT-Seq as a barcode decay-based method to unravel lineage-linked transcriptome plasticity Nature Communications, 2023-02-25;14(1):1085. 2023-02-25 [PMID: 36841849] (Bioassay, Human) Bioassay Human
Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense. Front Immunol, 2022-09-29;13(0):933329. 2022-09-29 [PMID: 36248858] (Bioassay, Complex Species Category) Bioassay Complex Species Category
SM Woo, S Kim, SU Seo, S Kim, JW Park, G Kim, YR Choi, K Hur, TK Kwon Inhibition of USP1 enhances anticancer drugs-induced cancer cell death through downregulation of survivin and miR-216a-5p-mediated upregulation of DR5 Cell Death & Disease, 2022-09-24;13(9):821. 2022-09-24 [PMID: 36153316] (Bioassay, Human) Bioassay Human
J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022-03-25;13(1):1606. 2022-03-25 [PMID: 35338135] (Bioassay, Human) Bioassay Human
BT Wang, T Kothambawa, L Wang, TJ Matthew, SE Calhoun, AK Saini, MF Kotturi, G Hernandez, EW Humke, MS Peterson, AM Sinclair, BA Keyt Multimeric Anti-DR5 IgM Agonist Antibody IGM-8444 Is a Potent Inducer of Cancer Cell Apoptosis and Synergizes with Chemotherapy and BCL-2 Inhibitor ABT-199 Molecular Cancer Therapeutics, 2021-10-28;0(0):. 2021-10-28 [PMID: 34711645] (Bioassay, Human) Bioassay Human
M Lee, E Shin, J Bae, Y Cho, JY Lee, YH Lee, BW Lee, ES Kang, BS Cha Dipeptidyl peptidase-4 inhibitor protects against non-alcoholic steatohepatitis in mice by targeting TRAIL receptor-mediated lipoapoptosis via modulating�hepatic dipeptidyl peptidase-4 expression Sci Rep, 2020-11-10;10(1):19429. 2020-11-10 [PMID: 33173107] (Cell Culture, Human) Cell Culture Human
K Landgraf, N Klöting, M Gericke, N Maixner, E Guiu-Jurad, M Scholz, AV Witte, F Beyer, JT Schwartze, M Lacher, A Villringer, P Kovacs, A Rudich, M Blüher, W Kiess, A Körner The Obesity-Susceptibility Gene TMEM18 Promotes Adipogenesis through Activation of PPARG Cell Rep, 2020-10-20;33(3):108295. 2020-10-20 [PMID: 33086065] (Bioassay, Mouse) Bioassay Mouse
H Forde, E Harper, KD Rochfort, RG Wallace, C Davenport, D Smith, PM Cummins TRAIL inhibits oxidative stress in human aortic endothelial cells exposed to pro-inflammatory stimuli Physiol Rep, 2020-10-01;8(20):e14612. 2020-10-01 [PMID: 33080110] (Cell Culture, Human) Cell Culture Human
Show All 54 Publications.

Review for TRAIL/TNFSF10 (375-TL) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using 375-TL:
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Other TRAIL/TNFSF10 375-TL
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Western Blot Control 06/01/2018




CommentsStained with mouse anti-human TRAIL (III6F; 1ug/mL) overnight at 4C, followed by IRdye 680 goat anti-mouse secondary (Licor#926-68070; 1:10,000).

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Verified Customer
Application: Western Blot Control


Gene Symbol TNFSF10