Recombinant Human TRAIL/TNFSF10 Protein


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Recombinant Human TRAIL/TNFSF10 Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-12 ng/mL.
The apoptotic effect of soluble trimeric rhTRAIL can be enhanced by oligimerization of the soluble rhTRAIL through the use of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). The ED50 for this effect is 1-4 ng/mL.
Note: This is one of multiple forms available for this protein. Check R&D Systems' website,, for a complete listing of the variants.
Mouse myeloma cell line, NS0-derived human TRAIL/TNFSF10 protein
Thr95-Gly281, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Structure / Form
Noncovalently-linked homotrimer and disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
21 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
24 kDa, reducing conditions
Reviewed Applications
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Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 20 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TRAIL/TNFSF10 Protein

  • Apo-2 ligand
  • APO2L
  • Apo-2Ltumor necrosis factor (ligand) family, member 10
  • APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
  • CD253 antigen
  • CD253
  • Protein TRAIL
  • TL2
  • TNF-related apoptosis-inducing ligand
  • TNFSF10
  • TRAILTNF-related apoptosis inducing ligand TRAIL
  • tumor necrosis factor (ligand) superfamily, member 10
  • tumor necrosis factor ligand superfamily member 10


TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the new TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is most homologous to FAS ligand, sharing 28% amino acid sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that tranduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified (1 - 3). Osteoprotegerin has been identified as a fifth TRAIL receptor (4).

  1. Golstein, P. (1997) Current Biology 7:R750.
  2. Wiley, S.R. et al. (1995) Immunity 3:673.
  3. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
  4. Emery, J. et al. (1998) J. Biol. Chem. 273:14363.

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Publications for TRAIL/TNFSF10 (375-TL)(44)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 3 applications: Bioassay, Cell Culture, Surface Plasmon Resonance (SPR.

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Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using 375-TL Applications Species
SM Woo, SU Seo, P Kubatka, KJ Min, TK Kwon Honokiol Enhances TRAIL-Mediated Apoptosis through STAMBPL1-Induced Survivin and c-FLIP Degradation Biomolecules, 2019;9(12):. 2019 [PMID: 31817770] (Cell Culture, Human) Cell Culture Human
M Hong, J Li, S Li, M M Almutair Acetylshikonin Sensitizes Hepatocellular Carcinoma Cells to Apoptosis through ROS-Mediated Caspase Activation Cells, 2019;8(11):. 2019 [PMID: 31752383] (Bioassay, Human) Bioassay Human
WY Kong, ZY Yee, CW Mai, CM Fang, S Abdullah, SC Ngai Zebularine and trichostatin A sensitized human breast adenocarcinoma cells towards tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-induced apoptosis Heliyon, 2019;5(9):e02468. 2019 [PMID: 31687564] (Cell Culture, Human) Cell Culture Human
SP O'Hara, PL Splinter, CE Trussoni, E Guicciardi, NP Splinter, MS Al Suraih, N Nasser-Gho, D Stollenwer, GJ Gores, NF LaRusso The transcription factor ETS1 promotes apoptosis resistance of senescent cholangiocytes by epigenetically up-regulating the apoptosis suppressor BCL2L1 J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31659122] (Bioassay, Human) Bioassay Human
EA Kim, JH Jang, EG Sung, IH Song, JY Kim, TJ Lee MiR-1208 Increases the Sensitivity to Cisplatin by Targeting TBCK in Renal Cancer Cells Int J Mol Sci, 2019;20(14):. 2019 [PMID: 31331056] (Cell Culture, Human) Cell Culture Human
SM Woo, KJ Min, SU Seo, S Kim, P Kubatka, JW Park, TK Kwon Axl Inhibitor R428 Enhances TRAIL-Mediated Apoptosis Through Downregulation of c-FLIP and Survivin Expression in Renal Carcinoma Int J Mol Sci, 2019;20(13):. 2019 [PMID: 31269715] (Bioassay, Human) Bioassay Human
EB Jo, YS Lee, H Lee, JB Park, H Park, YL Choi, D Hong, SJ Kim Combination therapy with c-met inhibitor and TRAIL enhances apoptosis in dedifferentiated liposarcoma patient-derived cells BMC Cancer, 2019;19(1):496. 2019 [PMID: 31126284] (Bioassay, Human) Bioassay Human
T Li, Y Yang, H Song, H Li, A Cui, Y Liu, L Su, IN Crispe, Z Tu Activated NK cells kill hepatic stellate cells via p38/PI3K signaling in a TRAIL-involved degranulation manner J. Leukoc. Biol., 2019;0(0):. 2019 [PMID: 30748035] (Bioassay, Human) Bioassay Human
BR Kim, SH Park, YA Jeong, YJ Na, JL Kim, MJ Jo, S Jeong, HK Yun, SC Oh, DH Lee RUNX3 enhances TRAIL-induced apoptosis by upregulating DR5 in colorectal cancer Oncogene, 2019;0(0):. 2019 [PMID: 30692634] (Bioassay, Human) Bioassay Human
S Tian, DH Liu, D Wang, F Ren, P Xia Aldehyde Dehydrogenase 1 (ALDH1) Promotes the Toxicity of TRAIL in Non-Small Cell Lung Cancer Cells via Post-Transcriptional Regulation of MEK-1 Expression Cell. Physiol. Biochem., 2018;51(1):217-227. 2018 [PMID: 30448845] (Bioassay, Human) Bioassay Human
Show All 44 Publications.

Review for TRAIL/TNFSF10 (375-TL) (1) 51

Average Rating: 5
(Based on 1 review)

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CommentsStained with mouse anti-human TRAIL (III6F; 1ug/mL) overnight at 4C, followed by IRdye 680 goat anti-mouse secondary (Licor#926-68070; 1:10,000).

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Gene Symbol TNFSF10