Cyclin D1 Antibody (SPM587)

Images

 
Biological Strategies: Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - HELLS expression and protein levels are modulated with YAP1/TEAD inhibition downstream of SHH signaling. Western blot showing ...read more
Immunohistochemistry-Paraffin: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Formalin-paraffin human Mantle Cell Lymphoma stained with Cyclin D1 Ab (Clone SPM587).
Flow Cytometry: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Flow Cytometric analysis of human Cyclin D1 on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (SPM587).
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - analysis of Cyclin D1 in human breast cancer cell line MDA-MB-231 cell lysate using anti-Cyclin D1. Image from verified customer review.
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Western Blot of cyclin D1 in (1) C2C12 (2) HepG2 & (3) NIH3T3 Lysate with Cyclin D1 Ab (SPM587)
Flow Cytometry: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Flow Cytometric analysis of human Cyclin D1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (SPM587).

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
SPM587
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Cyclin D1 Antibody (SPM587) Summary

Immunogen
Human recombinant full length cyclin D1 protein
Localization
Nuclear
Marker
G1-Cyclin & Mantle Cell Marker
Specificity
Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CCND1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Western Blot 0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
WB reported in a verified customer review.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-32840 in the following applications:

Publications
Read Publications using
NBP2-32840 in the following applications:

  • 1 publication
  • 1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml (NBP2-34816).

Alternate Names for Cyclin D1 Antibody (SPM587)

  • B-cell lymphoma 1 protein
  • BCL-1 oncogene
  • Bcl-1
  • BCL1D11S287E
  • CCND1
  • cyclin D1 (PRAD1: parathyroid adenomatosis 1)
  • Cyclin D1
  • Cyl-1
  • G1/S-specific cyclin D1
  • G1/S-specific cyclin-D1
  • PRAD1 oncogene
  • PRAD1
  • PRAD1B-cell CLL/lymphoma 1
  • U21B31

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for Cyclin D1 Antibody (NBP2-32840)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Flow, IHC-P, WB.


Filter By Application
Flow
(1)
IHC-P
(1)
WB
(2)
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Filter By Species
Human
(3)
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 7 of 7.
Publications using NBP2-32840 Applications Species
Ding Z, Qiu M, Alharbi M et al FOXO1 expression in chondrocytes modulates cartilage production and removal in fracture healing Bone Mar 4 2021 [PMID: 33662610] (Flow, Mouse)

Details:
Citation using the Alexa Fluor 488 version of this antibody.
Flow Mouse
Gul HF, Ilhan N, Ilhan N et al. The Combined Effect of Pomegranate Extract and Tangeretin on the DMBA-induced Breast Cancer Model The Journal of nutritional biochemistry Dec 13 2020 [PMID: 33326843] (WB, Rat) WB Rat
Shi Q, Li Y, Li S et al. LncRNA DILA1 inhibits Cyclin D1 degradation and contributes to tamoxifen resistance in breast cancer Nat Commun Nov 2 2020 [PMID: 33139730] (IHC-P, Human) IHC-P Human
Xu W, Chen S, Wang X et al. Methylprednisolone potentiates tetrandrine pharmacodynamics against human T lymphoblastoid leukemia MOLT-4 cells via regulation of NF-kappa B activation and cell cycle transition Steroids Aug 17 2020 [PMID: 32818521]
Xu W, Wu H, Chen S et al. Cytotoxic effects of vitamins K1, K2 and K3 against human T lymphoblastoid leukemia cells through apoptosis induction and cell cycle arrest Chem Biol Drug Des Apr 18 2020 [PMID: 32305047] (Human) Human
Robinson MH, Maximov V, Lallani S et al. Upregulation of the chromatin remodeler HELLS is mediated by YAP1 in Sonic Hedgehog Medulloblastoma Sci Rep 2019 Sep 20 [PMID: 31541170] (WB, Mouse) WB Mouse
Chelsky ZL, Yue P, Kondratyuk TP et al. A Resveratrol Analogue Promotes ERKMAPK-Dependent Stat3 Serine and Tyrosine Phosphorylation Alterations and Antitumor Effects In Vitro against Human Tumor Cells Mol. Pharmacol. 2015 Sep 01 [PMID: 26138072] (Human) Human

Review for Cyclin D1 Antibody (NBP2-32840) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-32840:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Cyclin D1 NBP2-32840
Enlarge
5
reviewed by:
Verified Customer
WB Human 01/22/2015
View

Summary

ApplicationWestern Blot
Sample Testedhuman MDAMB-231 whole cell lysate
SpeciesHuman
Lot595-1XP140415

Blocking

Blocking Details5% nonfat dry milk in TBS-T buffer incubated at RT for 0.5-1 hr

Primary Anitbody

Dilution Ratio1:1000 in TBS-T at 4C overnight

Secondary Antibody

Secondary DescriptionIRDye® 680LT Goat anti-Mouse IgG (H + L)
Secondary Manufacturer Cat#LI-COR Biosci cat # 926-68020
Secondary Concentration1:25000 in TBS-T buffer at TR for 30 min

Details

Detection NotesLI-COR Image System, auto scanning

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cyclin D1 Antibody (NBP2-32840) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34816AF350
Alexa Fluor 405 NBP2-34816AF405
Alexa Fluor 488 NBP2-34816AF488
Alexa Fluor 532 NBP2-34816AF532
Alexa Fluor 594 NBP2-34816AF594
Alexa Fluor 647 NBP2-34816AF647
Alexa Fluor 700 NBP2-34816AF700
Alexa Fluor 750 NBP2-34816AF750
Allophycocyanin NBP2-34816APC
Biotin NBP2-34816B
DyLight 350 NBP2-34816UV
DyLight 405 NBP2-34816V
DyLight 488 NBP2-34816G
DyLight 550 NBP2-34816R
DyLight 594 NBP2-34816DL594
DyLight 650 NBP2-34816C
DyLight 680 NBP2-34816FR
DyLight 755 NBP2-34816IR
FITC NBP2-34816F
HRP NBP2-34816H
Janelia Fluor 549 NBP2-34816JF549
Janelia Fluor 646 NBP2-34816JF646
PE NBP2-34816PE
PerCP NBP2-34816PCP

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Bioinformatics Tool for Cyclin D1 Antibody (NBP2-32840)

Discover related pathways, diseases and genes to Cyclin D1 Antibody (NBP2-32840). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Cyclin D1.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

IKK alpha says "no" to NFk beta
The nuclear factor kappa B (NFkB) is a ubiquitous transcription factor essential for the activation of immune and inflammatory responses. NFkB activity is inhibited when it is associated with IkB proteins in the cell cytoplasm. IkB proteins are phosph...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Role of RASSF1A in Death Receptor-Dependent Apoptosis
Death-receptor apoptosis, or cell death, is essential for cellular growth regulation; its disruption is expressed in a variety of cancers. We at Novus Biologicals are one of the leading antibody suppliersfor apoptosis and cancer research groups, and t...  Read full blog post.

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Recent Reviews

5
5
1
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2
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1
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Verified Customer
01/22/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CCND1
Uniprot