Cyclin D1 Antibody (SPM587)

Images

 
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Western Blot of cyclin D1 in (1) C2C12 (2) HepG2 & (3) NIH3T3 Lysate with Cyclin D1 Ab (SPM587)
Immunohistochemistry-Paraffin: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Formalin-paraffin human Mantle Cell Lymphoma stained with Cyclin D1 Ab (Clone SPM587).
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - analysis of Cyclin D1 in human breast cancer cell line MDA-MB-231 cell lysate using anti-Cyclin D1. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-P, IP
Clone
SPM587
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Cyclin D1 Antibody (SPM587) Summary

Immunogen
Human recombinant full length cyclin D1 protein
Localization
Nuclear
Marker
G1-Cyclin & Mantle Cell Marker
Specificity
Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CCND1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 1 - 2ug/500ug protein lysate
Application Notes
Human Chromosome Location: 11q13.2
ELISA and Biological Function Blockade (Order Ab without BSA & Azide) Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) ELISA (For coating, order NBP2-34816 (the BSA free version of NBP2-32840))
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-32840 in the following applications:

Publications
Read Publication using NBP2-32840.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Cyclin D1 Antibody (SPM587)

  • B-cell lymphoma 1 protein
  • BCL-1 oncogene
  • Bcl-1
  • BCL1D11S287E
  • CCND1
  • cyclin D1 (PRAD1: parathyroid adenomatosis 1)
  • Cyclin D1
  • Cyl-1
  • G1/S-specific cyclin D1
  • G1/S-specific cyclin-D1
  • PRAD1 oncogene
  • PRAD1
  • PRAD1B-cell CLL/lymphoma 1
  • U21B31

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cyclin D1 Antibody (NBP2-32840)(1)

We have publications tested in 1 confirmed species: Human.


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Review for Cyclin D1 Antibody (NBP2-32840) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-32840:
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(1)
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Images Ratings Applications Species Date Details
Western Blot Cyclin D1 NBP2-32840
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reviewed by:
Anonymous
WB Human 01/22/2015
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Summary

ApplicationWestern Blot
Sample Testedhuman MDAMB-231 whole cell lysate
SpeciesHuman
Lot595-1XP140415

Blocking

Blocking Details5% nonfat dry milk in TBS-T buffer incubated at RT for 0.5-1 hr

Primary Anitbody

Dilution Ratio1:1000 in TBS-T at 4C overnight

Secondary Antibody

Secondary DescriptionIRDye® 680LT Goat anti-Mouse IgG (H + L)
Secondary Manufacturer Cat#LI-COR Biosci cat # 926-68020
Secondary Concentration1:25000 in TBS-T buffer at TR for 30 min

Details

Detection NotesLI-COR Image System, auto scanning

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cyclin D1 Antibody (NBP2-32840) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-34816AF405
Alexa Fluor 488 Labeled NBP2-34816AF488
Alexa Fluor 647 Labeled NBP2-34816AF647
Alexa Fluor 700 Labeled NBP2-34816AF700
Allophycocyanin Labeled NBP2-34816APC
Biotin Labeled NBP2-34816B
DyLight 350 Labeled NBP2-34816UV
DyLight 405 Labeled NBP2-34816V
DyLight 488 Labeled NBP2-34816G
DyLight 550 Labeled NBP2-34816R
DyLight 650 Labeled NBP2-34816C
DyLight 680 Labeled NBP2-34816FR
DyLight 755 Labeled NBP2-34816IR
HRP Labeled NBP2-34816H
PE Labeled NBP2-34816PE
PerCP Labeled NBP2-34816PCP

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Discover related pathways, diseases and genes to Cyclin D1 Antibody (NBP2-32840). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Cyclin D1.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

IKK alpha says "no" to NFk beta
The nuclear factor kappa B (NFkB) is a ubiquitous transcription factor essential for the activation of immune and inflammatory responses. NFkB activity is inhibited when it is associated with IkB proteins in the cell cytoplasm. IkB proteins are phosph...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Role of RASSF1A in Death Receptor-Dependent Apoptosis
Death-receptor apoptosis, or cell death, is essential for cellular growth regulation; its disruption is expressed in a variety of cancers. We at Novus Biologicals are one of the leading antibody suppliersfor apoptosis and cancer research groups, and t...  Read full blog post.

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Recent Reviews

5
5
1
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0
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0
2
0
1
0

Anonymous
01/22/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CCND1
Entrez
Uniprot