Bax Antibody (6A7)


Western Blot: Bax Antibody (6A7) [NBP1-28566] - rOPN administration elevated the expression of autophagy-related proteins while suppressing apoptosis in rat brain at 24 h after SAH. The effects of rOPN on expression more
Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566] - Mouse retina with no pretreatment. IHC-F image submitted by a verified customer review
Western Blot: Bax Antibody (6A7) [NBP1-28566] - Analysis of lysates from human metastatic pancreatic cancer cell line L3.6pl using Bax Antibody (clone 6A7; Lot # B168-P780E). This image was submitted via reviews by a more
Western Blot: Bax Antibody (6A7) [NBP1-28566] - Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probled with Mouse Anti-Bax UNLB.

Product Details

Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, Flow, Func, IHC, IHC-Fr
0.1 mg/ml

Order Details

Bax Antibody (6A7) Summary

KLH-conjugated peptide corresponding to amino acids 12-24 located near the N-terminus common to human, mouse and rat Bax.
The monoclonal antibody 6A7 reacts with human, mouse and rat Bax but does not bind the soluble cytosolic form of Bax; however, treatment of cells with non-ionic detergents exposes the epitope and allows binding of 6A7 to monomeric forms of Bax but not Bax complexed with either Bcl-2 or Bcl-xL.
IgG1 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot < = 1 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Use in functional reported in scientific literature (PMID:31410057). Bax antibody validated for IHC-F, WB from a verified customer reviews.
Bax Knockout HeLa Cell Lysate
Reviewed Applications
Read 3 Reviews rated 5
NBP1-28566 in the following applications:

Read Publications using
NBP1-28566 in the following applications:

  • 1 publication
  • WB
    9 publications

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Borate buffered saline, pH 8.2
No Preservative
0.1 mg/ml
Protein A or G purified

Alternate Names for Bax Antibody (6A7)

  • apoptosis regulator BAX
  • Bax
  • BCL2-associated X protein
  • Bcl2-L-4
  • BCL2L4bcl2-L-4
  • Bcl-2-like protein 4


Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems. (2) It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment. (3) Bax is a member of the Bcl-2 family of apoptosis-associated proteins and, although it is similar in structure to Bcl-2, Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and subsequent cell death in a caspase-independent manner, presumably via their ion channel-forming activity. These channels may then function to promote a mitochondrial permeability transition or to puncture the outer mitochondrial membrane. In addition to forming homodimer, Bax can heterodimerize with Bcl-2 and Bcl-XL proteins. This heterodimerization between pro- and anti-apoptotic family members may serve a titration function and act as a sensor for the programmed cell death program. (4-13) The monoclonal antibody 2D2 is specific for human Bax. (1)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
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Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, Flow-CS, Flow-IC
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
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Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu, Mu, Rt(-)
Applications: WB, Flow, ICC/IF, Flow-IC
Species: Hu, Mu
Applications: WB, Simple Western, IP
Species: Hu
Applications: WB, IHC
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu, Mu, Rt, Ma
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO

Publications for Bax Antibody (NBP1-28566)(11)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: Func, WB.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-28566 Applications Species
Sun F, Du J, Li H et al. FABP4 inhibitor BMS309403 protects against hypoxia-induced H9c2 cardiomyocyte apoptosis through attenuating endoplasmic reticulum stress J. Cell. Mol. Med. Sep 7 2020 [PMID: 32896039] (WB, Rat) WB Rat
Tang H, Gamdzyk M, Huang L et al. Delayed recanalization after MCAO ameliorates ischemic stroke by inhibiting apoptosis via HGF/c-Met/STAT3/Bcl-2 pathway in rats Exp. Neurol. May 16 2020 [PMID: 32428505] (WB, Rat) WB Rat
Sun CM, Enkhjargal B, Reis C et al. Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats CNS Neurosci Ther Aug 22 2019 [PMID: 31436915] (WB, Rat) WB Rat
Gong X, Xu B, Zi L, Chen X, miR-625 reverses multidrug resistance in gastric cancer cells by directly targeting ALDH1A1 CMAR Jul 1 2019 [PMID: 31410057] (Func, Human) Func Human
Zheng W, Matei N, Pang J et al. Delayed recanalization at 3 days after permanent MCAO attenuates neuronal apoptosis through FGF21/FGFR1/PI3K/Caspase-3 pathway in rats Exp. Neurol. Jul 8 2019 [PMID: 31295445] (WB, Rat) WB Rat
Yu, CC;Li, CF;Chen, IH;Lai, MT;Lin, ZJ;Korla, PK;Chai, CY;Ko, G;Chen, CM;Hwang, T;Lee, SC;Sheu, JJ; YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo-/radio-resistance by suppressing caspase-mediated apoptosis J. Pathol. Apr 3 2019 [PMID: 30945298] (WB, Human) WB Human
Zhou X, Liu S, Lin X et al. Metformin Inhibit Lung Cancer Cell Growth and Invasion in Vitro as Well as Tumor Formation in Vivo Partially by Activating PP2A Med. Sci. Monit. Jan 29 2019 [PMID: 30693913] (WB, Human) WB Human
Xu D, Chen P, Wang B et al. NIX-mediated mitophagy protects against proteinuria-induced tubular cell apoptosis and renal injury. Am. J. Physiol. Renal Physiol. Sep 12 2018 [PMID: 30207166] (WB, Rat) WB Rat
Xie Z, Enkhjargal B, Wu L et al. Exendin-4 attenuates neuronal death via GLP-1R/PI3K/Akt pathway in early brain injury after subarachnoid hemorrhage in rats. Neuropharmacology. 2017 Oct 04 [PMID: 28986282] (WB, Rat) WB Rat
Shi X, Xu L, Doycheva DM et al. Sestrin2, as a negative feedback regulator of mTOR, provides neuroprotection by activation AMPK phosphorylation in neonatal hypoxic-ischemic encephalopathy in rat pups J. Cereb. Blood Flow Metab. 2016 Jul 05 [PMID: 27381825]
Show All 11 Publications.

Reviews for Bax Antibody (NBP1-28566) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-28566:
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Images Ratings Applications Species Date Details
Western Blot Bax NBP1-28566
reviewed by:
Hayk Simonyan
WB Mouse 06/23/2017


ApplicationWestern Blot
Sample Testedmouse liver
Western Blot Bax NBP1-28566
reviewed by:
WB Human 03/31/2014


ApplicationWestern Blot
FileView PDF
Immunohistochemistry-Frozen Bax NBP1-28566
reviewed by:
IHC-Fr Mouse 07/28/2012


Sample Testedmouse retina


Blocking Details10% goat serum in phosphate buffer was used for blocking

Primary Anitbody

Dilution Ratio300 times, over night, 4 degree

Secondary Antibody

Secondary DescriptionGoat anti-house
Secondary Manufacturer Cat#Invitrogen, A10521
Secondary Concentration300 times dilution


Detection NotesCy3, Leica DMI 6000 System, exposure time 400 ms, nega con: 2nd ab only
Fixation Details4% PFA fixation for over night at 4 degree.
Wash DescriptionPBST, 5 min, 5 times

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Bioinformatics Tool for Bax Antibody (NBP1-28566)

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Blogs on Bax. Showing 1-10 of 14 blog posts - Show all blog posts.

Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death
Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1,...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

Required proteins for p62/SQSTM1 regulation and a role for p62/SQSTM1 in neuronal autophagy
Autophagy is a crucial cellular process that clears the cell of protein aggregates, toxins, and damaged cell products. Accumulation of toxins, damaged cell products and unwanted proteins has been proven to play a role in aging and many forms of dis...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.
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Recent Reviews


Hayk Simonyan
Application: WB
Species: Mouse

Application: WB
Species: Human

Application: IHC-Fr
Species: Mouse


Gene Symbol BAX