STING/TMEM173 Antibody

Images

 
Western Blot: STING/TMEM173 Antibody [NBP2-24683] - Sting expression was induced in human breast MDA-MB-231 cells followed by Western blotting using Sting/TMEM173 antibody (1:1000, Novus). Only one specific band at an ...read more
Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Mouse lung tissue section using STING antibody at 1:150 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed ...read more
Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on THP-1 cells with NBP2-24683 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% ...read more
Western Blot: STING/TMEM173 Antibody [NBP2-24683] - Total protein from THP-1, HT-29, U2OS cells and human spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Human colon cancer tissue section using STING antibody at 1:100 dilution with detection employing HRP-conjugated secondary antibody. The signal was ...read more
Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Mouse lung tissue section using STING antibody at 1:150 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed ...read more
Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Analysis of STING in FFPE human breast tumor using an isotype control (top left) and this antibody (bottom left, right) at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Ca, Pm, RM, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

STING/TMEM173 Antibody Summary

Immunogen
A portion of amino acids 310-360 of human STING was used as the immunogen.
Predicted Species
Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TMEM173
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:200-1:400
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Immunocytochemistry 1:200
  • Immunofluorescence 1:200
Application Notes
.
Control
STING/TMEM173 Lysate (NBP2-66102)
Control Peptide
STING/TMEM173 Protein (NBP2-24683PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24683 in the following applications:

Publications
Read Publications using
NBP2-24683 in the following applications:

Reactivity Notes

Opossum, Zebrafish (83%), Xenopus (72%), Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Peptide affinity purified

Alternate Names for STING/TMEM173 Antibody

  • anti-STING
  • endoplasmic reticulum IFN stimulator
  • Endoplasmic reticulum interferon stimulator
  • ERIS
  • FLJ38577
  • hMITA
  • human STING
  • Mediator of IRF3 activation
  • MITA
  • mitochondrial mediator of IRF3 activation
  • mouse STING
  • MPYS
  • NET23
  • N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner
  • Stimulator of interferon genes protein
  • STING
  • STINGhSTING
  • TMEM173
  • transmembrane protein 173

Background

STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA from bacteria/viruses or the self (intrinsic) and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING has been suggested to interact with DDX58/RIG-I, MAVS, SSR2, RNF5, TRIM56, TBK1, IFIT1 and IFIT2. It generally localize to the cytoplasm, and membranes of cell, ER and mitochondria but in response to DNA stimulation, it translocate to the perinuclear region for interaction with TBK1 kinase. The latter phosphorylates STING at Ser-358 residue which results in its activation. STING executes its role by spotting and binding cyclic di-GMP / c-di-GMP and cyclic GMP-AMP /cGAMP which follows the activation of NF-kappa B and IRF3 transcriptional signaling pathways leading to the induction of Type I interferon response. STING has also been suggested to involve in cell death signaling pathways through its association with MHC-II and the activation of ERK pathway. Besides immune response to bacterial/viral pathogens, innate immune gene transcription has been of great importance in the elucidation of the causes of auto-inflammatory disease involving the sensing of self-DNA and the generation of effective antitumor adaptive immunity. STING regulated innate signaling has offered critical insights and new opportunities into the development of novel immunization regimes and therapeutics for treatment of infection, autoimmune disorders, inflammation and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STING/TMEM173 Antibody (NBP2-24683)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(5)
IHC
(1)
IHC-P
(1)
WB
(4)
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Filter By Species
Human
(7)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP2-24683 Applications Species
S B, K S, S A et al. RAD51 interconnects between DNA replication, DNA repair and immunity. NCBI-GEO dataSets. Feb 18 2017 [PMID: 28334891] (IHC, Human) IHC Human
Reinert LS, Lopusna K, Winther H et al. Sensing of HSV-1 by the cGAS-STING pathway in microglia orchestrates antiviral defence in the CNS. Nat Commun. Nov 10 2016 [PMID: 27830700] (Mouse) Mouse
Li T, Cheng H, Yuan H et al. Antitumor Activity of cGAMP via Stimulation of cGAS-cGAMP-STING-IRF3 Mediated Innate Immune Response. Sci Rep. [PMID: 26754564] (IHC-P, Mouse) IHC-P Mouse
Sun C, Schattgen SA, Pisitkun P et al. Evasion of Innate Cytosolic DNA Sensing by a Gammaherpesvirus Facilitates Establishment of Latent Infection J. Immunol. 2015 Jan 16 [PMID: 25595793] (ICC/IF, Human)

Details:
STING/TMEM173/MPYS antibody used for ICC-IF staining on methanol fixed monocyte/THP1 - derived macrophages that were infected with CMV (MOI 3) or KSHV (30 genomes/cell) for 4 hours (FIGURE 4C), and on, bone marrow-derived/BMDCs from C57BL/6 mice after in vitro infection using HSV-1/MOI3 and MHV68/ MOI 20 (FIGURE 5C).
ICC/IF Human
Hansen K, Prabakaran T, Laustsen A et al. Listeria monocytogenes induces IFN-B expression through an IFI16-, cGAS- and STING-dependent pathway. EMBO J. 2014 Jun 26 [PMID: 24970844] (ICC/IF, Human)

Details:
Figs 6, S7: PMA-differentiated THP1 macrophages transfected with FITC-labelled dsDNA, cyclic-di-AMP or infected with L monocytogenes. Note: In mock-infected cells, TMEM173/MPYS (STING) was detected widely in the cytoplasm. Cytosolic STING relocalized to punctate cytoplasmic structures in DNA-treated cells and co-localized with IFI16 and DNA. Cytosolic STING also formed foci in c-di-AMP treated cells, and STING redistributed to the site of bacterial DNA in infected cells.
ICC/IF Human
Chiliveru S, Rahbek SH, Jensen SK et al. Inflammatory cytokines break down intrinsic immunological tolerance of human primary keratinocytes to cytosolic DNA. J. Immunol. 2014 Mar 1 [PMID: 24489095] (ICC/IF, Human)

Details:
Human primary keratinocytes, Fig 4. STING was detected in the cytoplasm; after certain treatments STING was colocalized with DNA in the cytoplasm in specific foci. The results suggested that STING was part of a functional signalsome.
ICC/IF Human
Olagnier D, Scholte FE, Chiang C et al. Inhibition of dengue and chikungunya virus infections by RIG-I-mediated type I interferon-independent stimulation of the innate antiviral response. J Virol. [PMID: 24478443] (WB, Human) WB Human
Jakobsen MR, Bak RO, Andersen A et al. IFI16 senses DNA forms of the lentiviral replication cycle and controls HIV-1 replication. Proc Natl Acad Sci U S A 2013 Nov 26 [PMID: 24154727] (ICC/IF, Human) ICC/IF Human
Panchanathan R, Liu H, Xin D, Choubey D. Identification of a negative feedback loop between cyclic di-GMP-induced levels of IFI16 and p202 cytosolic DNA sensors and STING. Innate Immun. [PMID: 24131791] (WB, Mouse) WB Mouse
Horan KA, Hansen K, Jakobsen MR et al. Proteasomal degradation of herpes simplex virus capsids in macrophages releases DNA to the cytosol for recognition by DNA sensors. J Immunol. 2013 Mar 1 [PMID: 23345332]
Show All 12 Publications.

Review for STING/TMEM173 Antibody (NBP2-24683) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24683:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot STING/TMEM173 NBP2-24683
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reviewed by:
Anonymous
WB Human 10/29/2018
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Summary

ApplicationWestern Blot
Sample TestedHuman MDA-MB-231 cells
SpeciesHuman
LotA-3

Comments

CommentsPerformed on multiple human cancer cells with and without Sting induction. Specific band detected ~37 kDa without other non-specific bands. Primary antibody was used at 1:1000 in 5% milk in IBS-T with traditional HRP detection.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STING/TMEM173 Antibody (NBP2-24683) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on STING/TMEM173.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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Recent Reviews

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Anonymous
10/29/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol TMEM173
Uniprot