STING/TMEM173 Antibody - BSA Free

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Western Blot: STING/TMEM173 Antibody [NBP2-24683] - STING/TMEM173 expression was induced in human breast MDA-MB-231 cells followed by Western blotting using STING/TMEM173 Antibody antibody (1:1000). Only one specific ...read more
Immunocytochemistry/ Immunofluorescence: STING/TMEM173 Antibody [NBP2-24683] - RH-30 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells ...read more
Western Blot: STING/TMEM173 Antibody [NBP2-24683] - Total protein from THP-1, HT-29, U2OS cells and human spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Human colon cancer tissue section using STING/TMEM173 Antibody at 1:100 dilution with detection employing HRP-conjugated secondary antibody. The ...read more
Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Mouse lung tissue section using STING/TMEM173 Antibody at 1:150 dilution with detection employing HRP-conjugated secondary antibody. The signal was ...read more
Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on U937 cells with NBP2-24683AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683] - An intracellular stain was performed on THP-1 cells with STING/TMEM173 Antibody NBP2-24683AF647 (blue) and a matched isotype control NBP2-24891 (orange). ...read more
Immunocytochemistry/ Immunofluorescence: STING/TMEM173 Antibody [NBP2-24683] - HT-29 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells ...read more
Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on THP-1 cells with STING/TMEM173 Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized ...read more
Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on RH30 cells with STING/TMEM173 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Summary
Reactivity Hu, Mu, Ca, Pm, RM, BvSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

STING/TMEM173 Antibody - BSA Free Summary

Immunogen
Partial synthetic peptide made to an internal portion of human STING/TMEM173 (between amino acids 310-360) [UniProt Q86WV6]
Predicted Species
Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TMEM173
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-5 ug/million cells
  • Immunocytochemistry/Immunofluorescence 5 - 10 ug/ml
  • Immunohistochemistry 1:100 - 1:300
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:300
  • Western Blot 1 - 2 ug/ml
Application Notes
Use in ELISA reported in scientific literature (PMID:34905508).Use in IHC-Fr reported in scientific literature (PMID:33745949)..
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
STING/TMEM173 Protein (NBP2-24683PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24683 in the following applications:

Publications
Read Publications using
NBP2-24683 in the following applications:

Reactivity Notes

Opossum, Zebrafish (83%), Xenopus (72%), Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Peptide affinity purified

Alternate Names for STING/TMEM173 Antibody - BSA Free

  • anti-STING
  • endoplasmic reticulum IFN stimulator
  • Endoplasmic reticulum interferon stimulator
  • ERIS
  • FLJ38577
  • hMITA
  • hSTING
  • human STING
  • Mediator of IRF3 activation
  • MITA
  • mitochondrial mediator of IRF3 activation
  • mouse STING
  • MPYS
  • NET23
  • N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner
  • SAVI
  • Stimulator of interferon genes protein
  • stimulator of interferon protein
  • STING
  • STING-beta
  • TMEM173
  • transmembrane protein 173

Background

STING (stimulator of interferon genes) is encoded by the TMEM173 gene and is an adaptor molecule involved in the activation of innate immune responses to PAMPS (pathogen-associated molecular patterns) and DAMPS (damage-associated molecular patterns). STING specifically recognizes cytosolic DNA products derived from pathogens (e.g., cytomegalovirus, vaccinia virus, Listeria monocytogenes) or dead cells (1, 2). In the STING pathway, dsDNA derived from pathogens or damaged cells serves as a substrate for the enzyme cGAS (cyclic GMP-AMP synthase) which produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP (3, 4). Under steady-state conditions STING (theoretical molecular weight 42 kDa), a protein localizes to the ER membrane. Upon activation by dsDNA derived second messenger (cGAMP), STING translocates to the Golgi apparatus as a homodimer. Once STING has trafficked to the perinuclear region, it activates TANK binding kinase 1 (TBK1), interferon regulatory factor 3 (IRF3) and NF-?B leading to the production of cytokines (e.g., type I interferon) (2, 4). Mutations in the TMEM173 gene affecting STING expression are associated with the development of the auto-inflammatory disease SAVI (STING-associated vasculopathy with onset in infancy) (2). A novel SAVI dominant mutation in the TMEM173 human gene (V155M) leads to increased localization of STING to the Golgi and perinuclear region, indicative of an activated state (1). Hallmarks of SAVI, a rare inflammatory disease, include severe vasculitis in extremities and lung inflammation (7).

References

1. Patel, S., & Jin, L. (2019). TMEM173 variants and potential importance to human biology and disease. Genes and Immunity. https://doi.org/10.1038/s41435-018-0029-9

2. Jounai, N., Kobiyama, K., Takeshita, F., & Ishii, K. J. (2013). Recognition of damage-associated molecular patterns related to nucleic acids during inflammation and vaccination. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2012.00168

3. Xiao, T. S., & Fitzgerald, K. A. (2013). The cGAS-STING Pathway for DNA Sensing. Molecular Cell. https://doi.org/10.1016/j.molcel.2013.07.004

4. Kato, K., Omura, H., Ishitani, R., & Nureki, O. (2017). Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA. Annual Review of Biochemistry. https://doi.org/10.1146/annurev-biochem-061516-044813

5. Crowl, J. T., Gray, E. E., Pestal, K., Volkman, H. E., & Stetson, D. B. (2017). Intracellular Nucleic Acid Detection in Autoimmunity. Annual Review of Immunology. https://doi.org/10.1146/annurev-immunol-051116-052331

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STING/TMEM173 Antibody (NBP2-24683)(27)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(1)
Flow
(3)
ICC/IF
(8)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(9)
All Applications
Filter By Species
Human
(9)
Mouse
(11)
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Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NBP2-24683 Applications Species
Ding R, Li H, Liu Y et al. Activating cGAS-STING axis contributes to neuroinflammation in CVST mouse model and induces inflammasome activation and microglia pyroptosis Journal of neuroinflammation Jun 10 2022 [PMID: 35689216] (ICC/IF, Mouse) ICC/IF Mouse
Gupta U, Ghosh S, Wallace CT et al. Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD Autophagy [PMID: 35473441]
Li X, Zhu Y, Zhang X et al. An alternatively spliced STING isoform localizes in the cytoplasmic membrane and directly senses extracellular cGAMP The Journal of clinical investigation Dec 14 2021 [PMID: 34905508] (WB, Flow, ELISA, Mouse) WB, Flow, ELISA Mouse
He Y, Hong C, Yan EZ et al. Self-assembled cGAMP-STING delta TM signaling complex as a bioinspired platform for cGAMP delivery Sci Adv Jun 1 2020 [PMID: 32582856]
Hoang A, Yu H, Lin Y Et al. Immune privilege of adipocyte mitochondria protects from obesity Research Square Nov 4 2021 (Flow) Flow
Jeong SH, Yang MJ, Choi S et al. Refractoriness of STING therapy is relieved by AKT inhibitor through effective vascular disruption in tumour Nature communications Jul 20 2021 [PMID: 34285232] (WB) WB
Kim JS, Cho E, Mun SJ et al. Multi-Functional MPT Protein as a Therapeutic Agent against Mycobacterium tuberculosis Biomedicines May 13 2021 [PMID: 34068051]
Shmuel-Galia L, Humphries F, Lei X et al. Dysbiosis exacerbates colitis by promoting ubiquitination and accumulation of the innate immune adaptor STING in myeloid cells Immunity May 25 2021 [PMID: 34051146] (Flow, Mouse)

Details:
Flow cytometry was performed using the STING/TMEM173 antibody in mice. The flow allowed for the profiling of immune cell populations in the lamina propria (LP) and intra epithelial lymphocyte (IEL) compartments of young N153s and WT mice.
Flow Mouse
Inoue Y, Ayaki T, Ishimoto T et al. The stimulator of interferon genes (STING) pathway is upregulated in striatal astrocytes of patients with multiple system atrophy Neuroscience letters May 24 2021 [PMID: 34033888]
Jiang GL, Yang XL, Zhou HJ et al. cGAS knockdown promotes microglial M2 polarization to alleviate neuroinflammation by inhibiting cGAS-STING signaling pathway in cerebral ischemic stroke Brain research bulletin Mar 18 2021 [PMID: 33745949] (WB, IHC-Fr, Mouse) WB, IHC-Fr Mouse
Show All 27 Publications.

Review for STING/TMEM173 Antibody (NBP2-24683) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24683:
Filter by Applications
Western Blot
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot STING/TMEM173 NBP2-24683
Enlarge
5
reviewed by:
Mark Esposito
Western Blot Human 10/29/2018
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Summary

ApplicationWestern Blot
Sample TestedHuman MDA-MB-231 cells
SpeciesHuman
LotA-3

Comments

CommentsPerformed on multiple human cancer cells with and without Sting induction. Specific band detected ~37 kDa without other non-specific bands. Primary antibody was used at 1:1000 in 5% milk in IBS-T with traditional HRP detection.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STING/TMEM173 Antibody (NBP2-24683). (Showing 1 - 1 of 1 FAQs).

  1. I’m curious about the mouse reactivity of your STING/TMEM173/MPYS antibody, Catalog Number NBP2-24683.
    • For our product NBP2-24683, the mouse reactivity for this product was determined by homology. The immunogen sequence for this antibody shares 94% homology to the mouse protein.

Secondary Antibodies

 

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Blogs on STING/TMEM173.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ...  Read full blog post.

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Recent Reviews

5
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Mark Esposito
10/29/2018
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol TMEM173
Uniprot