STING/TMEM173 Antibody [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Ca, Pm, RM, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Order Details

STING/TMEM173 Antibody [Janelia Fluor® 549] Summary

Immunogen
Partial synthetic peptide made to an internal portion of human STING/TMEM173 (between amino acids 310-360) [UniProt Q86WV6]
Predicted Species
Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TMEM173
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Opossum, Zebrafish (83%), Xenopus (72%), Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for STING/TMEM173 Antibody [Janelia Fluor® 549]

  • endoplasmic reticulum IFN stimulator
  • Endoplasmic reticulum interferon stimulator
  • ERIS
  • FLJ38577
  • hMITA
  • hSTING
  • Mediator of IRF3 activation
  • MITA
  • mitochondrial mediator of IRF3 activation
  • MPYS
  • NET23
  • N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner
  • SAVI
  • Stimulator of interferon genes protein
  • stimulator of interferon protein
  • STING
  • STING-beta
  • TMEM173
  • transmembrane protein 173

Background

STING (stimulator of interferon genes) is encoded by the TMEM173 gene and is an adaptor molecule involved in the activation of innate immune responses to PAMPS (pathogen-associated molecular patterns) and DAMPS (damage-associated molecular patterns). STING specifically recognizes cytosolic DNA products derived from pathogens (e.g., cytomegalovirus, vaccinia virus, Listeria monocytogenes) or dead cells (1, 2). In the STING pathway, dsDNA derived from pathogens or damaged cells serves as a substrate for the enzyme cGAS (cyclic GMP-AMP synthase) which produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP (3, 4). Under steady-state conditions STING (theoretical molecular weight 42 kDa), a protein localizes to the ER membrane. Upon activation by dsDNA derived second messenger (cGAMP), STING translocates to the Golgi apparatus as a homodimer. Once STING has trafficked to the perinuclear region, it activates TANK binding kinase 1 (TBK1), interferon regulatory factor 3 (IRF3) and NF-?B leading to the production of cytokines (e.g., type I interferon) (2, 4). Mutations in the TMEM173 gene affecting STING expression are associated with the development of the auto-inflammatory disease SAVI (STING-associated vasculopathy with onset in infancy) (2). A novel SAVI dominant mutation in the TMEM173 human gene (V155M) leads to increased localization of STING to the Golgi and perinuclear region, indicative of an activated state (1). Hallmarks of SAVI, a rare inflammatory disease, include severe vasculitis in extremities and lung inflammation (7).

References

1. Patel, S., & Jin, L. (2019). TMEM173 variants and potential importance to human biology and disease. Genes and Immunity. https://doi.org/10.1038/s41435-018-0029-9

2. Jounai, N., Kobiyama, K., Takeshita, F., & Ishii, K. J. (2013). Recognition of damage-associated molecular patterns related to nucleic acids during inflammation and vaccination. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2012.00168

3. Xiao, T. S., & Fitzgerald, K. A. (2013). The cGAS-STING Pathway for DNA Sensing. Molecular Cell. https://doi.org/10.1016/j.molcel.2013.07.004

4. Kato, K., Omura, H., Ishitani, R., & Nureki, O. (2017). Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA. Annual Review of Biochemistry. https://doi.org/10.1146/annurev-biochem-061516-044813

5. Crowl, J. T., Gray, E. E., Pestal, K., Volkman, H. E., & Stetson, D. B. (2017). Intracellular Nucleic Acid Detection in Autoimmunity. Annual Review of Immunology. https://doi.org/10.1146/annurev-immunol-051116-052331

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STING/TMEM173 Antibody (NBP2-24683JF549) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-24683AF350
Alexa Fluor 405 NBP2-24683AF405
Alexa Fluor 488 NBP2-24683AF488
Alexa Fluor 532 NBP2-24683AF532
Alexa Fluor 594 NBP2-24683AF594
Alexa Fluor 647 NBP2-24683AF647
Alexa Fluor 700 NBP2-24683AF700
Alexa Fluor 750 NBP2-24683AF750
Allophycocyanin/Cy7 NBP2-24683APCCY7
Biotin NBP2-24683B
DyLight 350 NBP2-24683UV
DyLight 405 NBP2-24683V
DyLight 488 NBP2-24683G
DyLight 550 NBP2-24683R
DyLight 594 NBP2-24683DL594
DyLight 650 NBP2-24683C
DyLight 680 NBP2-24683FR
DyLight 755 NBP2-24683IR
FITC NBP2-24683F
HRP NBP2-24683H
Janelia Fluor 549 NBP2-24683JF549
Janelia Fluor 646 NBP2-24683JF646
PE/Atto594 NBP2-24683PEATT594
PE/Cy5.5 NBP2-24683PECY55
PE/Cy7 NBP2-24683PECY7

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Diseases for STING/TMEM173 Antibody (NBP2-24683JF549)

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Blogs on STING/TMEM173.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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Bioinformatics

Gene Symbol TMEM173