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MAVS Antibody

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Immunohistochemistry-Paraffin: MAVS Antibody [NB100-80859] - Staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunohistochemistry-Paraffin: MAVS Antibody [NB100-80859] - Staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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MAVS Antibody Summary

Immunogen
Synthetic peptide from human MAVS / VISA.
Specificity
17 amino acid peptide from near the center of human VISA.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAVS
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Theoretical MW
56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-80859 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAVS Antibody

  • CARD adapter inducing interferon beta
  • CARD adapter inducing interferon-beta
  • CARD adaptor inducing IFN-beta
  • Cardif
  • DKFZp547C224
  • DKFZp666M015
  • FLJ27482
  • IFN-B promoter stimulator 1
  • Interferon beta promoter stimulator protein 1
  • interferon-beta promoter stimulator protein 1
  • IPS-1
  • IPS-1FLJ41962
  • IPS1MGC3260
  • KIAA1271FLJ35386
  • MAVS
  • mitochondrial antiviral signaling protein
  • mitochondrial antiviral-signaling protein
  • mitochondrial viral signaling protein
  • Putative NF-kappa-B-activating protein 031N
  • virus-induced signaling adapter variant 1b
  • virus-induced signaling adaptor variant 1a
  • Virus-induced-signaling adapter
  • VISA
  • VISAFLJ38051

Background

MAVS (Mitochondrial Antiviral Signaling) mediates the activation of NF-kappaB and IRF3 in response to antiviral infection. Silencing of MAVS expression permits derepression of viral replication, while over expression of MAVS boosts antiviral immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: RIG-I Antibody [NBP2-27419] - Analysis of 293 lysate in the 1) absence and 2) presence of immunizing peptide using RIG-I antibody at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: RIG-I Antibody [NBP2-27419] - Formalin-fixed, paraffin-embedded human breast stained with RIG-I antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: RIG-I Antibody [NBP2-27419] - Formalin-fixed, paraffin-embedded human uterus stained with RIG-I antibody at 10 ug/ml.
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Species: Hu, Pm
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IRF3 Antibody
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Western Blot: IRF3 Antibody (2G3) [NBP1-47812] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3.
Immunocytochemistry/Immunofluorescence: IRF3 Antibody (2G3) [NBP1-47812] Staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF3.
Immunohistochemistry-Paraffin: IRF3 Antibody (2G3) [NBP1-47812] - Staining of paraffin-embedded pancreas tissue using anti-IRF3 mouse monoclonal antibody.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, Single Cell Western
MDA5 Antibody
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Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244] - IHC analysis of MDA5 staining in paraffin embedded Human Pancreas using (10ug/ml). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
(8 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
NHE6/SLC9A6
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Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-32463] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-32463] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-32463] - Staining in human cerebral cortex and liver tissues using anti-SLC9A6 antibody. Corresponding SLC9A6 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
TRIF/TICAM1
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NB120-13810
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse spleen probed with TRIF antibody, in the A) presence and B) absence of immunizing peptide, at 1:1000.
Immunocytochemistry/Immunofluorescence: TRIF/TICAM1 Antibody [NB120-13810] - TRIF antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.
Western Blot: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of TRIF in mouse A) intestine, B) spleen and C) RAW lysate using TRIF antibody at 1:1000 dilution.
(26 Publications)
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, IB, ICC/IF, IP
TBK1 Antibody
(108A429)
NB100-56705
Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis using Azide and BSA Free version of NB100-56705. Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.
Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-TBK1 at 20 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Analysis using Azide and BSA Free version of NB100-56705. Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE))
(1 Review)
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NFkB p50 (NLS)
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NBP2-29323
Functional (Inhibition): NFkB p105/p50 Inhibitor Peptide Set [NBP2-29323] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29323) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).
(10 Publications)
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
TLR3 Antibody
(40C1285.6)
NBP2-24875
Western Blot: TLR3 Antibody (40C1285.6) [NBP2-24875] - analysis of TLR3 in lysates from A) untransfected 293, B) 293 cells transfected with human TLR3 cDNA, C) human intestine, D) placenta, E) heart and F) ovary using TLR3 antibody at 3 ug/ml. Goat anti-mouse HRP conjugate was used as secondary.
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KRAS Antibody
(3B10-2F2)
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(1 Review)
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Applications: WB, ELISA, ICC/IF, IP
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NBP1-85348
Western Blot: DHX58 Antibody [NBP1-85348] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-163
Immunohistochemistry-Paraffin: DHX58 Antibody [NBP1-85348] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
(1 Publication)
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Western Blot: RNF36 Antibody [NBP2-42073] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
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TRAF-3 Antibody
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Immunohistochemistry-Paraffin: TRAF-3 Antibody [NB100-56176] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using TRAF-3 antibody at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
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NBP2-26551
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TNF-alpha
[Unconjugated]
210-TA
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IRF7 Antibody
NBP1-77263
Western Blot: IRF7 Antibody [NBP1-77263] - 293 whole cell lysate with IRF7 antibody at (A) 0.5 and (B) 1 ug/ml and (C) 2 ug/ml.
Immunocytochemistry/Immunofluorescence: IRF7 Antibody [NBP1-77263] - Mouse Spleen cells using IRF7 antibody at 20 ug/ml.
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(2 Publications)
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MyD88 Inhibitor
Peptide Set
NBP2-29328
MyD88 Inhibitor Peptide Set
(3 Reviews)
(64 Publications)
Species: Hu, Mu, Rt, Xp, Ye, Ze
Applications: ELISA

Publications for MAVS Antibody (NB100-80859) (0)

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Review for MAVS Antibody (NB100-80859) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-80859:
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reviewed by:
Juan Pablo de Rivero Vaccari
 WB Rat 09/22/2010
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Summary

ApplicationWestern Blot
Sample Testedprimary astrocytes
SpeciesRat

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WB Video Protocol

FAQs for MAVS Antibody (NB100-80859). (Showing 1 - 1 of 1 FAQ).

  1. Molecular weight of MAVS?
    • MAVS is a 540 aa protein with a predicted size of 56 kDa.

Secondary Antibodies

 

Isotype Controls

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STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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 Juan Pablo de Rivero Vaccari
09/22/2010
Application: WB
Species: Rat
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Bioinformatics

Gene Symbol MAVS
Entrez
Uniprot