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MAVS Antibody

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Western Blot: MAVS Antibody [NB100-80859] - Rat brain tissue lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Western Blot: MAVS Antibody [NB100-80859] - Rat brain tissue lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: MAVS Antibody [NB100-80859] - Mouse Brain cells with VISA antibody at 20 ug/ml.
Immunohistochemistry-Paraffin: MAVS Antibody [NB100-80859] - Staining of human brain, cortex tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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MAVS Antibody Summary

Immunogen
A 17 amino acid synthetic peptide near the center of human MAVS. The immunogen is located within amino acids 130 - 180 of MAVS.
Epitope
Near the center, aa 130-180
Specificity
17 amino acid peptide from near the center of human MAVS.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAVS
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-2 ug/ml
Theoretical MW
56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-80859 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Peptide affinity purified

Alternate Names for MAVS Antibody

  • CARD adapter inducing interferon beta
  • CARD adapter inducing interferon-beta
  • CARD adaptor inducing IFN-beta
  • Cardif
  • DKFZp547C224
  • DKFZp666M015
  • FLJ27482
  • IFN-B promoter stimulator 1
  • Interferon beta promoter stimulator protein 1
  • interferon-beta promoter stimulator protein 1
  • IPS-1
  • IPS-1FLJ41962
  • IPS1MGC3260
  • KIAA1271FLJ35386
  • MAVS
  • mitochondrial antiviral signaling protein
  • mitochondrial antiviral-signaling protein
  • mitochondrial viral signaling protein
  • Putative NF-kappa-B-activating protein 031N
  • virus-induced signaling adapter variant 1b
  • virus-induced signaling adaptor variant 1a
  • Virus-induced-signaling adapter
  • VISA
  • VISAFLJ38051

Background

MAVS (Mitochondrial Antiviral Signaling) mediates the activation of NF-kappaB and IRF3 in response to antiviral infection. Silencing of MAVS expression permits derepression of viral replication, while over expression of MAVS boosts antiviral immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: RIG-I Antibody [NBP2-27419] - Analysis of 293 lysate in the 1) absence and 2) presence of immunizing peptide using RIG-I antibody at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
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Immunocytochemistry/Immunofluorescence: IRF3 Antibody (SD2062) [NBP2-67741] - Staining IRF3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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TRIF/TICAM1 Antibody [NB120-13810] - Lysates of heart tissue were immunoprecipitated with anti-TLR3 antibodies (IP: TLR3), followed by SDS-PAGE and immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non-specific binding of antibodies to cellular proteins. Green arrows indicate non-specific bands. The association between TLR3 and Trif, but not MyD88, was detectable in sham myocardium and was increased in infarct. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13328) licensed under a CC-BY licence. Anti-TLR3 antibody by WB (NB100-56571)
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Western Blot: DHX58 Antibody [NBP1-85348] - (A) HeLa and (B) C2C12 cells were treated with the indicated siRNAs for 2 days and cellular lysates were subjected to Western blot analysis using anti-rapsyn antibody. GAPDH was used as loading control. Rapsyn protein levels are reduced in NUP88-depleted cells. Quantification of the respective expression levels of rapsyn after transfection of HeLa and C2C12 cells with the indicated siRNAs and shRNA-mediated depletion of NUP88 in C2C12 cells. Blots from three independent experiments for each condition were analyzed. Data present mean +/- SEM. P-values: ***<0.001; **<0.01, *<0.05; t-test, one-tailed. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1007845) licensed under a CC-BY licence.
Immunohistochemistry: DHX58 Antibody [NBP1-85348] - Bright-field images of histological muscle sections from individual B.II.2 and a control fetus stained with anti-rapsyn antibodies (brown). Nuclei were visualized by hematoxylin. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1007845) licensed under a CC-BY licence.
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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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(36 Reviews)
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(SC0617)
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Immunocytochemistry/Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634] - Staining IRF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of paraffin-embedded human kidney tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
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MyD88 Inhibitor
Peptide Set
NBP2-29328
MyD88 Inhibitor Peptide Set
(3 Reviews)
(88 Publications)
Species: Bv, Hu, Mu, Rt, Xp, Ye, Ze
Applications: BindInhib, B/N, ELISA, Flow, Func-Inh, IHC, In vitro, In vivo
MAVS Antibody
NB100-80859
MAVS Antibody
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for MAVS Antibody (NB100-80859) (0)

There are no publications for MAVS Antibody (NB100-80859).
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Review for MAVS Antibody (NB100-80859) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-80859:
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  5
reviewed by:
Juan Pablo de Rivero Vaccari
 WB Rat 09/22/2010
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Summary

ApplicationWestern Blot
Sample Testedprimary astrocytes
SpeciesRat

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAVS Antibody (NB100-80859). (Showing 1 - 1 of 1 FAQ).

  1. Molecular weight of MAVS?
    • MAVS is a 540 aa protein with a predicted size of 56 kDa.

Secondary Antibodies

 

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Blogs on MAVS.

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STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ...  Read full blog post.

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 Juan Pablo de Rivero Vaccari
09/22/2010
Application: WB
Species: Rat
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Bioinformatics

Gene Symbol MAVS
Entrez
Uniprot