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MAVS Antibody - BSA Free

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Western Blot: MAVS Antibody - BSA Free [NB100-80859] - Rat brain tissue lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Western Blot: MAVS Antibody - BSA Free [NB100-80859] - Rat brain tissue lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: MAVS Antibody - BSA Free [NB100-80859] - Mouse Brain cells with VISA antibody at 20 ug/ml.
Immunohistochemistry-Paraffin: MAVS Antibody - BSA Free [NB100-80859] - Staining of human brain, cortex tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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MAVS Antibody - BSA Free Summary

Immunogen
A 17 amino acid synthetic peptide near the center of human MAVS. The immunogen is located within amino acids 130 - 180 of MAVS.
Epitope
Near the center, aa 130-180
Specificity
17 amino acid peptide from near the center of human MAVS.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAVS
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-2 ug/ml
Theoretical MW
56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-80859 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Peptide affinity purified

Alternate Names for MAVS Antibody - BSA Free

  • CARD adapter inducing interferon beta
  • CARD adapter inducing interferon-beta
  • CARD adaptor inducing IFN-beta
  • Cardif
  • DKFZp547C224
  • DKFZp666M015
  • FLJ27482
  • IFN-B promoter stimulator 1
  • Interferon beta promoter stimulator protein 1
  • interferon-beta promoter stimulator protein 1
  • IPS-1
  • IPS-1FLJ41962
  • IPS1MGC3260
  • KIAA1271FLJ35386
  • MAVS
  • mitochondrial antiviral signaling protein
  • mitochondrial antiviral-signaling protein
  • mitochondrial viral signaling protein
  • Putative NF-kappa-B-activating protein 031N
  • virus-induced signaling adapter variant 1b
  • virus-induced signaling adaptor variant 1a
  • Virus-induced-signaling adapter
  • VISA
  • VISAFLJ38051

Background

MAVS (Mitochondrial Antiviral Signaling) mediates the activation of NF-kappaB and IRF3 in response to antiviral infection. Silencing of MAVS expression permits derepression of viral replication, while over expression of MAVS boosts antiviral immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of A172 human glioblastoma cell line and Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RIG-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4859) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RIG-I at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Species: Hu
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IRF3 Antibody
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, Raji human Burkitt's lymphoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IRF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4019) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IRF3 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and IRF3 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IRF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4019) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IRF3 at approximately 50 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and IRF3 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for IRF3 at approximately 57 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. Goat Anti-Human IRF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4019)was used at 10 µg/mL followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Species: Hu
Applications: CyTOF-ready, ICC, ICFlow, KO, Simple Western, WB
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Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244] - Staining in paraffin embedded Human Pancreas. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: MDA5 Antibody [NB110-1244] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Species: Hu, Mu, Pm
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IFN-beta
[Unconjugated]
8499-IF/CF
Recombinant Human IFN-beta (Catalog # 8499-IF/CF) exhibits anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 5-30 pg/mL.
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NHE6/SLC9A6
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NBP2-38877
Immunocytochemistry/Immunofluorescence: NHE6/SLC9A6 Antibody [NBP2-38877] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-38877] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-38877] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
TRIF/TICAM1
Antibody - BSA Free
NB120-13810
Knockdown Validated: TRIF/TICAM1 Antibody [NB120-13810] - siRNA was transfected at 50 nM for 48 hrs, and poly(I:C) was added at 100 ug/ml for 4 hrs before cell harvest. Negative control (NC) siRNA served as control. RNAiMAX transfection reagent was used in all the siRNA experiments. The panel shows the knockdown effect of Trif siRNA. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13328) licensed under a CC-BY licence.
TRIF/TICAM1 Antibody [NB120-13810] - Lysates of heart tissue were immunoprecipitated with anti-TLR3 antibodies (IP: TLR3), followed by SDS-PAGE and immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non-specific binding of antibodies to cellular proteins. Green arrows indicate non-specific bands. The association between TLR3 and Trif, but not MyD88, was detectable in sham myocardium and was increased in infarct. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13328) licensed under a CC-BY licence. Anti-TLR3 antibody by WB (NB100-56571)
Immunohistochemistry-Paraffin: TRIF/TICAM1 Antibody [NB120-13810] - Analysis of a FFPE tissue section of mouse spleen using 1:200 dilution of TRIF/TICAM1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
(29 Publications)
Species: Mu, Po, Rt
Applications: IB, ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB
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(108A429) - BSA Free
NB100-56705
Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Analysis of FFPE human testis tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:200. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin.
Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x.
Simple Western: TBK1 Antibody (108A429) [NB100-56705] - Lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody
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(29 Publications)
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NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
TLR3 Antibody
(40C1285.6) - BSA Fre
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Flow Cytometry: TLR3 Antibody (40C1285.6) [NBP2-24875] - Expression of TLR3 protein on epithelial cells. HNEC (A), Detroit-562 (B) and FaDu (C) were stained intracellularly with FITC-Abs against TLR3 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0098239), licensed under a CC-BY licence.
Western Blot: TLR3 Antibody (40C1285.6) [NBP2-24875] - All the cell lines displayed a strong expression of TLR3 protein. Cell lysates were electrophoresed and blotted to PVDF membrane, which was probed with TLR3-specific antibody. To confirm the immunoreactivity of the antibody, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY licence.
Flow Cytometry: TLR3 Antibody (40C1285.6) [NBP2-24875] - Expression of TLR3 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR3 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR3. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY licence.
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Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - miRNA-768-3p specifically targets K-ras. Effect of miRNA inhibitors that were reduced by co-culture on the level of pan-RAS, phospho-B-Raf and CD44 and pan-Akt by western blotting. Also shown is the effect of different miRNA inhibitors on the level of K-ras in H520 cells by western blot. Cropped blots are shown and were run under the same experimental conditions. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep02392), licensed under a CC-BY licence.
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.Lane 1: KRAS transfected lysate(21 KDa).Lane 2: Non-transfected lysate.
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.
(1 Review)
(13 Publications)
Species: Hu, Mu
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DHX58 Antibody
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Western Blot: DHX58 Antibody [NBP1-85348] - Rig-I Like Receptor (RLR) gene expression in stable cell lines encompassing ST-RLRs. Western blot analysis was performed using (D) anti-LGP2 in ST-RLR cells. Cells were transfected with pIFNB-FLuc, pTK-Rluc and either mock, poly(I:C) or 5'3P. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/11275) licensed under a CC-BY licence.
Western Blot: DHX58 Antibody [NBP1-85348] - (A) HeLa and (B) C2C12 cells were treated with the indicated siRNAs for 2 days and cellular lysates were subjected to Western blot analysis using anti-rapsyn antibody. GAPDH was used as loading control. Rapsyn protein levels are reduced in NUP88-depleted cells. Quantification of the respective expression levels of rapsyn after transfection of HeLa and C2C12 cells with the indicated siRNAs and shRNA-mediated depletion of NUP88 in C2C12 cells. Blots from three independent experiments for each condition were analyzed. Data present mean +/- SEM. P-values: ***<0.001; **<0.01, *<0.05; t-test, one-tailed. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1007845) licensed under a CC-BY licence.
Immunohistochemistry: DHX58 Antibody [NBP1-85348] - Bright-field images of histological muscle sections from individual B.II.2 and a control fetus stained with anti-rapsyn antibodies (brown). Nuclei were visualized by hematoxylin. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1007845) licensed under a CC-BY licence.
(1 Publication)
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Applications: IHC, IHC-P, WB
RNF36 Antibody
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Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207] - Staining of paraffin embedded Human Cerebellum at dilution of 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: RNF36 Antibody [NB100-1207] - (0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, PEP-ELISA, WB
TRAF-3 Antibody
NB100-56176
Immunohistochemistry-Paraffin: TRAF-3 Antibody - Unpurified [NB100-56176] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using this at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
(1 Publication)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
IFN-alpha 1
Antibody
NBP2-75930
Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.
Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(3 Reviews)
(739 Publications)
Species: Hu
Applications: BA
IRF7 Antibody
(SC0617)
NBP2-67634
Immunocytochemistry/Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634] - Staining IRF7 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: IRF7 Antibody (SC0617) [NBP2-67634] - Staining IRF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IRF7 Antibody (SC0617) [NBP2-67634] - Analysis of paraffin-embedded human kidney tissue using anti-IRF7 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
MyD88 Inhibitor
Peptide Set
NBP2-29328
MyD88 Inhibitor Peptide Set
(3 Reviews)
(91 Publications)
Species: Bv, Hu, Mu, Rt, Xp, Ye, Ze
Applications: BindInhib, B/N, ELISA, Flow, Func-Inh, IHC, In vitro, In vivo
MAVS Antibody -
BSA Free
NB100-80859
MAVS Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for MAVS Antibody (NB100-80859) (0)

There are no publications for MAVS Antibody (NB100-80859).
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Review for MAVS Antibody (NB100-80859) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-80859:
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  5
reviewed by:
Verified Customer
 WB Rat 09/22/2010
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Summary

ApplicationWestern Blot
Sample Testedprimary astrocytes
SpeciesRat

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAVS Antibody (NB100-80859). (Showing 1 - 1 of 1 FAQ).

  1. Molecular weight of MAVS?
    • MAVS is a 540 aa protein with a predicted size of 56 kDa.

Secondary Antibodies

 

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STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ...  Read full blog post.
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 Verified Customer
09/22/2010
Application: WB
Species: Rat
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Bioinformatics

Gene Symbol MAVS
Entrez
Uniprot