STING/TMEM173 Antibodies: Novus Biologicals

NBP2-24683

STING/TMEM173 Antibody - BSA Free

(1 Review)

(38 Publications)

0.1 mg

NBP2-24683

Western Blot: STING/TMEM173 Antibody [NBP2-24683] - Total protein from THP-1, HT-29, U2OS cells and human spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml STING/TMEM173 Antibody in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Human colon cancer tissue section using STING/TMEM173 Antibody at 1:100 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated very weak cytoplasmic staining in columnar epithelial cells with a very strong signal in the secretory/goblet cells.

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Mouse lung tissue section using STING/TMEM173 Antibody at 1:150 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated mainly a cytoplasmic staining in the bronchiolar and alveolar epithelial cells.

Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on U937 cells with NBP2-24683AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Flow Cytometry: STING/TMEM173 Antibody - BSA Free [NBP2-24683] - An intracellular stain was performed on THP-1 cells with STING/TMEM173 Antibody NBP2-24683AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody [NBP2-24683] - HT-29 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with STING/TMEM173 Antibody at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on THP-1 cells with STING/TMEM173 Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Flow Cytometry: STING/TMEM173 Antibody [NBP2-24683] - An intracellular stain was performed on RH30 cells with STING/TMEM173 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of THP-1 cell line and STING/TMEM173 knockout THP-1 cell line (KO). Nitrocellulose membrane was probed with STING/TMEM173 Antibody (Catalog # NBP2-24683) followed by an HRP-conjugated secondary antibody. A specific band was detected for STING/TMEM173 at approximately 41 kDa (as indicated) in the parental THP-1 cell line, but is not detectable in knockout THP-1 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

$PMA-treated THP-1 lysates were prepared and immunoprecipitation was performed using 2.0 µg of STING/TMEM173 Antibody (Catalog # NBP2-24683) pre-coupled to Dynabeads protein A. Immunoprecipitated STING/TMEM173 was detected with STING/TMEM173 Antibody (Catalog # NBP3-18816). For western blot, NBP3-18816 was used at 1/1000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

<b>Genetic Strategies Validation. </b>PMA-treated THP-1 WT and STING/TMEM173 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with STING/TMEM173 Antibody (Catalog # NBP2-24683) and with an Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

STING pathway of tumour cells is not required for cGAMP-induced tumour EC apoptosis.a, b mRNA and protein levels of STING in cultured LLC cells transduced with nothing (NOT), shControl (shCon) or shSTING. Each dot indicates a value from one sample and n = 3 from two independent experiments. Vertical bars indicate mean +/- SD. c–e Diagram depicting generation of implanted LLC tumour by injection of the LLC cells transduced with NOT, shCon or shSTING, and treatment schedule of i.t. PBS or cGAMP in B6 mice. Comparison of LLC tumour growths. n = 6 mice/group from three independent experiments. Dots and bars indicate mean +/- SD. Plot indicates each individual tumour growth. f–i Diagram depicting generation of implanted LLC tumour by injection of the LLC cells transduced with shCon or shSTING, i.t. PBS or cGAMP treatment, and sampling of tumours at 24 h later. Representative images and comparisons of apoptosis in tumour ECs and whole tumour cells (whole cells). White arrowheads indicate apoptotic ECs. Scale bars, 1.0 mm (yellow) and 100 μm (white). Each dot indicates a value from one mouse and n = 6 mice from four independent experiments. Vertical bars indicate mean +/- SD. P values by Welch’s one-way ANOVA test followed by Dunnett’s T3 test (a, d, i). ****P < 0.0001; ns, not significant. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34285232), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

cGAS, STING and HMGB‐1 expressions in HT22 cells after OGD/R and melatonin treatment. (A) Quantitative evaluation and representative Western blots of cGAS, STING (B) and nuclear (C) and cytosolic (D) HMGB‐1 expressions in untreated HT22 cells (Ctrl), 8‐h OGD‐exposed cells (OGD) or followed by 15 min, 30 min or 1 h of reoxygenation (OGD/R) and 8‐h OGD‐exposed cells followed by 15 min, 30 min or 1 h of reoxygenation in the presence of 50 μM melatonin (OGD/R + Mel). Data normalised to the loading control beta ‐actin or lamin A are expressed as % of control and are the mean +/- SD (N = 3 independent experiments performed in triplicate); *p < 0.05, **p < 0.01, §p < 0.001 vs Ctrl; **p < 0.01, §p < 0.001, bars. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39707673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Combination of diABZI and BRAF inhibitors prevent NRF2 activation and activate STING. (A) Immunoblot analysis of phospho STING upon treatment with diABZI and BRAFis, Dabrafenib (DF) and Vemurafenib (VF). (B) Densitometric quantification of pSTING (n = 3). (C) Immunoblot analysis of phospho IRF3 upon treatment with diABZI and BRAFis, Dabrafenib (DF) and Vemurafenib (VF). (D) Densitometric quantification of pIRF3 (n = 3). (E) Immunoblot analysis of NRF2 upon diABZI and BRAFi treatment in C32 cells. (F) Densitometric quantification of NRF2 (n = 3). (G) Immunoblot of cytosolic and nuclear cell fractions upon treatment with diABZI and BRAFis, Dabrafenib (DF) and Vemurafenib (VF). **P < 0.01, ***P < 0.001, ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32477956), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Low-dose GCV inhibited cGAS-STING pathways in RAW264.7 cells. (A–C) Q-PCR analysis showed the effect of pretreatment of GCV on up-regulation of mRNA expression of Sting1, Il10, Ifnb1 induced by CMA- (A), DMXAA- (B), and cGAMP (C) in RAW264.7 cells. (D) Western blotting analysis showed the effect of GCV on CMA-induced expression changes of cGAS, STING, IFN-beta , and p-TBK1 in RAW264.7 cells. (E) Statistical analysis results for (D). (F) Western blotting analysis showed the effect of GCV on DMXAA-induced expression changes of cGAS, STING, IFN-beta , and p-TBK1 in RAW264.7 cells. (G) Statistical analysis results for (F). (H) Western blotting analysis showed the effect of GCV on cGAMP-induced expression changes of cGAS, STING, IFN-beta , and p-TBK1 in RAW264.7 cells. (I) Statistical analysis results for (H). (n = 4 each group, *p < 0.05, **p < 0.01, ***p < 0.001 vs. saline. &p < 0.05, &&p < 0.01, &&&p < 0.001 vs. STING agonists group, unpaired Student’s t-test). (n = 4 each group, *p < 0.05, **p < 0.01, ***p < 0.001, &&p < 0.01, &&&p < 0.001; unpaired Student’s t-test). All data was expressed as Mean +/- SEM. VEH, vehicle. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36467059), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

STING was upregulated following DSS-induced chronic colitis in mice and in UC patients. (A) Body weight loss was induced by DSS-colitis in mice. (B) The quantification of area under the curve (AUC) for (A). (C) DSS-colitis induced changes of the Disease activity index (DAI) score. (D) The quantification of AUC for (C). (E) DSS-colitis induced mechanical pain hypersensitivity in the abdomen. (F) The quantification of AUC for (E). (n = 7-8 per group; *p < 0.05, **p < 0.05, ***p < 0.001, DSS vs. vehicle group; two-way ANOVA with post-hoc Bonferroni test). (G) Representative pictures showed colon shortening induced by DSS. (H) The quantification of colon shortening for (G). (n = 7-8 per group; ***p < 0.001, DSS vs. vehicle group; unpaired Student’s t-test). (I) Representative H&E staining of colon sections from DSS group and vehicle group. (J) Statistical analysis for (I). (n = 4 each group, ***p < 0.001, DSS vs. vehicle group; unpaired Student’s t-test). (K) Double immunostaining of STING and F4/80 in the colon tissue from DSS group and vehicle group. (L) Statistical analysis for (K), scale bar: 50 μm. (n = 4 each group, *p < 0.05, DSS vs. vehicle group; unpaired Student’s t-test). (M) Western blotting analysis of STING expression in colon of mice. (N) Statistical analysis of Western blotting. (n = 3 each group, *p < 0.05, DSS vs. vehicle group; unpaired Student’s t-test). (O) The expression of STING in the colon tissue was increased in patients with ulcerative colitis compared with adjacent tissue of colon cancer. (P) Statistical analysis results for (O). (n = 4; **p < 0.01 vs Normal; unpaired Student’s t-test). The scale bar: 20 μm. All data was expressed as Mean +/- SEM. DAI, disease activity index; DSS, dextran sulfate sodium. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36467059), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

STING deficiency attenuated DSS-colitis in mice. (A) Representative Western blotting detected cGAS and STING expression in STINGgt/gt and WT mice. (B) Statistical analysis for (A). (C) Representative immunofluorescence detected STING and F4/80 expression in STINGgt/gt and WT mice. (D) Statistical analysis for (C). (n = 3 each group, **p < 0.01, STINGgt/gt vs. vehicle group; unpaired Student’s t-test). Wild-type (WT) and STINGgt/gt mice were given 3% dextran sulfate sodium (DSS) in drinking water for 8 days. (E) Body weight changed following DSS administration in mice. (F) The quantification of AUC for (E). (G) DSS-induced changes of DAI score in WT and STINGgt/gt mice. (H) The quantification of AUC for (G). (I) DSS-induced changes of mechanical pain sensitivity in the abdomen in STINGgt/gt mice and WT mice. (J) The quantification of AUC for (I). (n = 5-7 per group; **p < 0.01, ***p < 0.001, DSS vs. vehicle group; #p < 0.05, ##p < 0.01, ###p < 0.001, STINGgt/gt + DSS vs DSS group; two-way ANOVA with post-hoc Bonferroni test). (K) Representative pictures of colons from WT and STINGgt/gt mice on day 8. (L) Quantification of the colon length in (K). (n = 5-7 per group; ***p < 0.001, ###p < 0.001; unpaired Student’s t-test). (M) Representative photographies of H&E staining of colon sections from 4 different groups. (N) Statistical analysis for (M) (n = 4 each group; ***p < 0.001, #p < 0.05; unpaired Student’s t-test). (O) The Q-PCR analysis of mRNA expression of colonic Cgas, Il10, Ifnb1, Cxcl10, Il1b, and Tnf in mice from different group (n = 6 each group, **p < 0.01, ***p < 0.001, #p < 0.05, ##p < 0.01, ###p < 0.001; unpaired Student’s t-test). All data was expressed as Mean +/- SEM. DSS, dextran sulfate sodium; n.s., no significance. WT, wild type. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36467059), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Low-dose GCV attenuated cGAS-STING pathways in the colon of DSS-colitis in mice. (A) Western blotting analysis showed that protein expression of colonic STING, cGAS, p-TBK1, IFN-beta , IL-1 beta , and TNF-alpha in mice of different treatment group. (B) Statistical analysis for (A) (n = 4 per group, *p < 0.05, **p < 0.01, DSS vs. vehicle group; #p < 0.05, ##p < 0.01, ###p < 0.001, GCV + DSS vs. DSS group; unpaired Student’s t-test). (C) Double immunostaining of STING and F4/80 in the colon tissue. (D) Statistical results for (C). Scale bar = 50 μm. (n = 4 each group, ***p < 0.001, ##p < 0.01; unpaired Student’s t-test). All data was expressed as Mean +/- SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36467059), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

diABZI activates STING in melanoma cells. (A) Immunoblot analysis of STING pathway in untreated vs. diABZI treated samples (n = 3). (B) Densitometric analysis of phosphorylated STING (pSTING) and (C) Densitometric analysis of phosphorylated TBK1 (pTBK1) (n = 3). (D) Immunofluorescence staining and confocal microscopy analysis of pSTING in untreated vs. diABZI treatment. Arrows indicate perinuclear localization of pSTING. (E) Immunoblot of phosphorylated IRF3 in diABZI treated samples. Scale bar = 10 μm; **P < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32477956), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of STING and cGAS mRNA and protein in adipocytes. (A) Venn diagram summarizing the number of equally and differently expressed mRNA transcripts of young and adult mouse iAT. A gene network associated with Sting1 was equally expressed by young and adult iAT. A protein–protein interaction map, generated by STRING [30] is shown below the Venn diagram. Extended analysis presented in [24]. (B) Immunofluorescence of in vitro cultured adipocytes from young and adult mouse iAT; nc: nucleus, scale bar 20 μm. (C) Immunostaining of STING and cGAS proteins in the iAT of young mice, showing a region containing both multilocular and unilocular adipocytes. Arrowheads label nuclei; lp: lipid droplet; cyt: cytoplasm; scale bar: 50 μm. (D) Top: Expression of STING1 and CGAS mRNA in human inguinal and abdominal adipose tissue specimens. Linear regression analysis indicates a significant positive correlation between STING1 and CGAS mRNA levels. Each data point represents one tissue donor patient. Bottom: Correlation of donor age and the adipose tissue expression levels of STING1 and CGAS. (E) Immunohistochemistry of STING and cGAS proteins in human adipose tissue, collected from the inguinal-low abdominal region. Nineteen-month-old male infant; arrowheads label nuclei; lp: lipid droplet; cyt: cytoplasm; scale bar: 25 μm. Inlet shows nuclear STING labeling of an in vitro cultured human adipocyte. Scale bar: 20 μm. (F) Body mass index z-score (BMI z-score) and BMI standard deviation score (BMI-SDS) of adipose tissue donors involved in this study. Correlation of BMI z-score with adipose tissue STING1 and CGAS mRNA levels. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37830559), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Pm, RM, Bv

Species Glossary

Reactivity: Hu, Mu, Pm, RM, Bv

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6485A

Alt Catalog# IMG-6485A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24683AF350
Alexa Fluor 405	NBP2-24683AF405
Alexa Fluor 488	NBP2-24683AF488
Alexa Fluor 532	NBP2-24683AF532
Alexa Fluor 594	NBP2-24683AF594
Alexa Fluor 647	NBP2-24683AF647
Alexa Fluor 700	NBP2-24683AF700
Alexa Fluor 750	NBP2-24683AF750
Alexa Fluor Plus 405	NBP2-24683AFP405
Alexa Fluor Plus 488	NBP2-24683AFP488
Alexa Fluor Plus 555	NBP2-24683AFP555
Alexa Fluor Plus 594	NBP2-24683AFP594
Alexa Fluor Plus 647	NBP2-24683AFP647
Alexa Fluor Plus 680	NBP2-24683AFP680
Allophycocyanin	NBP2-24683APC
Allophycocyanin/Cy7	NBP2-24683APCCY7
Biotin	NBP2-24683B
CoraFluor 1	NBP2-24683CL1
DyLight 350	NBP2-24683UV
DyLight 405	NBP2-24683V
DyLight 488	NBP2-24683G
DyLight 550	NBP2-24683R
DyLight 594	NBP2-24683DL594
DyLight 650	NBP2-24683C
DyLight 680	NBP2-24683FR
DyLight 755	NBP2-24683IR
FITC	NBP2-24683F
HRP	NBP2-24683H
Janelia Fluor 525	NBP2-24683JF525
Janelia Fluor 549	NBP2-24683JF549
Janelia Fluor 646	NBP2-24683JF646
Janelia Fluor 669	NBP2-24683JF669
PE	NBP2-24683PE
PE/Atto594	NBP2-24683PEATT594
PE/Cy5.5	NBP2-24683PECY55
PE/Cy7	NBP2-24683PECY7
PerCP	NBP2-24683PCP
mFluor Violet 450 SE	NBP2-24683MFV450
mFluor Violet 500 SE	NBP2-24683MFV500
mFluor Violet 610 SE	NBP2-24683MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24683SS

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MAB7169

STING/TMEM173 Antibody (723505) [Unconjugated]

(1 Review)

(31 Publications)

100 μg

MAB7169

HAF007). A specific band was detected for STING/TMEM173 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF007). A specific band was detected for STING/TMEM173 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for STING/TMEM173 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (<a class=

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (<a class=

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (<a class=

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

STING/TMEM173 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CYLD interacts with STING in a ubiquitination-dependent manner.(A) HEK293T cells were transfected with the indicated plasmids. Thirty-six hours after transfection, the cell lysates were immunoprecipitated with an anti-Myc antibody and then immunoblotted with the indicated antibodies. (B) HEK293T cells were transfected with the indicated plasmids. Thirty-six hours after transfection, cell lysates were immunoprecipitated with an anti-HA antibody and then immunoblotted with the indicated antibodies. (C and D) HEK293T cells were transfected with the indicated plasmids. Thirty-six hours after transfection, the cell lysates were immunoprecipitated with an anti-Myc antibody (C) or an anti-HA antibody (D) and then immunoblotted with the indicated antibodies. (E) HEK293T cells were transfected with the indicated plasmids. Thirty-six hours after transfection, the cell lysates were immunoprecipitated with an anti-Myc antibody and then immunoblotted with the indicated antibodies. (F) After stimulation with HSV-1 (MOI = 10) for the indicated time periods in the presence of MG132 (10 μM), lysates of MEFs were immunoprecipitated with an anti-STING antibody and then immunoblotted with the indicated antibodies. (G) HeLa cells were transfected with empty vector or Flag-tagged RNF5 for 24 h and then stained with the indicated antibodies before imaging by confocal microscopy. STING (Green) (R&D Systems); CYLD (Red) (Abcam); Nucleus (Blue). Scale bars represent 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30388174), licensed under a CC-BY license. Not internally tested by R&D Systems.

HCT116 cells surviving PARP1 depletion activate innate immune signaling.(A-B) RNA-Seq data from HCT116EV and HCT116PARP1-/- cells (clones C2 and C4) was analyzed using Gene Set Enrichment Analysis (GSEA). The top category of differentially expressed genes was “Interferon Alpha Response”. The enrichment plot is shown in (A) and the heat map for the 97 mRNAs in this category in shown in (B). Map was generated using Cufflinks software (version 2.2.1) and shows absolute expression values independently normalized and analyzed for each comparison pair (EV/C2 and EV/C4). (C) The same RNA-Seq dataset was analyzed using Ingenuity Pathway Analysis (IPA). A representative plot highlighting enrichment for Interferon-Stimulated Genes (ISGs) is shown. (D) The induction of multiple ISGs observed by RNA-Seq was confirmed by q-RT-PCR. Bars represent the average and standard deviation of quadruplicate samples in each experiment and data is representative of 2–4 independent experiments. (E-F) The induction of factors involved in the sensing/signaling of cytoplasmic nucleic acids observed by RNA-Seq was confirmed by Q-RT-PCR (E). Bars represent the average and standard deviation of quadruplicate samples in each experiment and data is representative of 2–3 independent experiments. Protein expression for the same factors was assessed by immunoblotting (F). Blots are representative of 2–3 independent experiments. (G) Fixed cells were stained with an antibody to IRF3 and counterstained with DAPI. Images are representative of 5 random fields per slide. The experiment was repeated twice with similar results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29590171), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>ISG induction in cells surviving PARP1 depletion is dependent on RIG-I and MAVS.(A-E) To assess the efficiency of RNA silencing to STING (A), RIG-I (B), MDA-5 (C), TLR3 (D) or MAVS (E), cells transfected with the specific pooled siRNAs and control cells transfected with a scrambled siRNA or untreated were harvested 4 days after transfection and probed with the indicated antibodies. GAPDH (A, B, C, E) or alpha -tubulin (D) served as loading controls. In blots where multiple bands are observed (A, D, E), black arrows point to specific bands and asterisks to unspecific bands. (F) The expression of ISGs OAS1 and IFIT3 was quantified by qRT-PCR after knock down. Data is normalized to the expression in HCT116EV cells (= 1). Bars represent the average and standard deviation of quadruplicates. Data is representative of two independent experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29590171), licensed under a CC-BY license. Not internally tested by R&D Systems.

$PMA-treated THP-1 lysates were prepared, and immunoprecipitation was performed using 2.0 μg of Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) pre-coupled to Dynabeads protein G. Immunoprecipitated STING/TMEM173 was detected with a Rabbit Anti-STING/TMEM173 antibody. For western blot, the Rabbit Anti-STING/TMEM173 antibody was used at 1/1000. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. *=monoclonal antibody, **=recombinant antibody. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC7169U-100UG
Alexa Fluor 405	IC7169V-100UG
Alexa Fluor 488	IC7169G-025
Alexa Fluor 532	FAB7169X
Alexa Fluor 594	IC7169T-100UG
Alexa Fluor 647	IC7169R-100UG
Alexa Fluor 700	IC7169N-100UG
Alexa Fluor 750	IC7169S-100UG
Alexa Fluor Plus 405	FAB7169AFP405-100UG
Alexa Fluor Plus 488	FAB7169AFP488-100UG
Alexa Fluor Plus 555	FAB7169AFP555-100UG
Alexa Fluor Plus 594	FAB7169AFP594-100UG
Alexa Fluor Plus 647	FAB7169AFP647-100UG
Alexa Fluor Plus 680	FAB7169AFP680-100UG
Allophycocyanin	IC7169A-025
Biotin	FAB7169B
CoraFluor 1	FAB7169CL1
DyLight 350	FAB7169D
DyLight 405	FAB7169E
DyLight 488	FAB7169K
DyLight 550	FAB7169L
DyLight 594	FAB7169M
DyLight 650	FAB7169W
DyLight 680	FAB7169Y
DyLight 755	FAB7169Z
FITC	FAB7169F
HRP	FAB7169H
Janelia Fluor 525	FAB7169JF525
Janelia Fluor 549	FAB7169I
Janelia Fluor 646	FAB7169J
Janelia Fluor 669	FAB7169JF669
Phycoerythrin	IC7169P-025
mFluor Violet 450 SE	FAB7169MFV450
mFluor Violet 500 SE	FAB7169MFV500
mFluor Violet 610 SE	FAB7169MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7169-SP
Carrier Free	IC7169P-025
Carrier Free	IC7169A-025
Carrier Free	IC7169G-025
Carrier Free	IC7169R-100UG
Carrier Free	IC7169N-100UG
Carrier Free	IC7169V-100UG
Carrier Free	IC7169U-100UG
Carrier Free	IC7169T-100UG
Carrier Free	IC7169S-100UG

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NBP3-18816

STING/TMEM173 Antibody (2922D) - BSA Free

0.1 mg

NBP3-18816

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.The image is attached." class="big_lightbox">

STING/TMEM173 was detected in immersion fixed paraffin-embedded sections of human brain Cortex (Cerebrum) using Rabbit Anti-Human STING/TMEM173, Monoclonal Antibody (Catalog #NBP3-18816) at 10ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.The image is attached." alt="STING/TMEM173 was detected in immersion fixed paraffin-embedded sections of human brain Cortex (Cerebrum) using Rabbit Anti-Human STING/TMEM173, Monoclonal Antibody (Catalog #NBP3-18816) at 10ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.The image is attached." class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

STING was detected in frozen sections of mouse hippocampus using Rabbit Anti-Mouse STING Monoclonal Antibody (Catalog # NBP3-18816) at 10ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent preprocessing by incubating tissue with Multi Staining Buffer (Lunaphore Catalog # BU06) for 5minutes at room temperature followed by a 20-minute incubation in Tris-Buffered Saline + 0.2% Triton at room temperature. Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

STING was detected in frozen sections of mouse cerebral cortex using Rabbit Anti-Mouse STING Monoclonal Antibody (Catalog # NBP3-18816) at 10ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent preprocessing by incubating tissue with Multi Staining Buffer (Lunaphore Catalog # BU06) for 5minutes at room temperature followed by a 20-minute incubation in Tris-Buffered Saline + 0.2% Triton at room temperature. Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

STING was detected in frozen sections of mouse choroid plexus using Rabbit Anti-Mouse STING Monoclonal Antibody (Catalog # NBP3-18816) at 10ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent preprocessing by incubating tissue with Multi Staining Buffer (Lunaphore Catalog # BU06) for 5minutes at room temperature followed by a 20-minute incubation in Tris-Buffered Saline + 0.2% Triton at room temperature. Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

Western Blot: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Western blot shows cell lysates K562, THP-1, C2C12, and C6. Membrane was probed with 1 ug/mL of (Catalog # NBP3-18816) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.

Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed U937 human myeloid leukaemia cell line but is not detected in Daudi cell line using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # NBP3-18816) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue).

Immunohistochemistry: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed paraffin-embedded sections of mouse lung tissue using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pneumocytes and alveolar cells. Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Simple Western Lane view shows K562 and THP-1 whole cell lysate (WCL). A specific band was detected for hSTING/TMEM173 antibody (NBP3-18816) at approximately 44 kDa (as indicated) using 10 ug/mL of hSTING/TMEM173 antibody. This experiment was conducted under reducing conditions.

$PMA-treated THP-1 lysates were prepared and immunoprecipitation was performed using 2.0 µg of STING/TMEM173 Antibody (Catalog # NBP3-18816) pre-coupled to Dynabeads protein A. Immunoprecipitated STING/TMEM173 was detected with NBP3-18816. For western blot, NBP3-18816 was used at 1/1000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-18816AF350
Alexa Fluor 405	NBP3-18816AF405
Alexa Fluor 488	NBP3-18816AF488
Alexa Fluor 532	NBP3-18816AF532
Alexa Fluor 594	NBP3-18816AF594
Alexa Fluor 647	NBP3-18816AF647
Alexa Fluor 700	NBP3-18816AF700
Alexa Fluor 750	NBP3-18816AF750
Alexa Fluor Plus 405	NBP3-18816AFP405
Alexa Fluor Plus 488	NBP3-18816AFP488
Alexa Fluor Plus 555	NBP3-18816AFP555
Alexa Fluor Plus 594	NBP3-18816AFP594
Alexa Fluor Plus 647	NBP3-18816AFP647
Alexa Fluor Plus 680	NBP3-18816AFP680
Allophycocyanin	NBP3-18816APC
Biotin	NBP3-18816B
CoraFluor 1	NBP3-18816CL1
DyLight 350	NBP3-18816UV
DyLight 405	NBP3-18816V
DyLight 488	NBP3-18816G
DyLight 550	NBP3-18816R
DyLight 594	NBP3-18816DL594
DyLight 650	NBP3-18816C
DyLight 680	NBP3-18816FR
DyLight 755	NBP3-18816IR
FITC	NBP3-18816F
HRP	NBP3-18816H
Janelia Fluor 525	NBP3-18816JF525
Janelia Fluor 549	NBP3-18816JF549
Janelia Fluor 646	NBP3-18816JF646
Janelia Fluor 669	NBP3-18816JF669
PE	NBP3-18816PE
PerCP	NBP3-18816PCP
mFluor Violet 450 SE	NBP3-18816MFV450
mFluor Violet 500 SE	NBP3-18816MFV500
mFluor Violet 610 SE	NBP3-18816MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-18816SS
Azide and BSA Free	NBP3-18817

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AF6516

STING/TMEM173 Antibody [Unconjugated]

(11 Publications)

100 μg

AF6516

HAF016). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human/Mouse STING/TMEM173 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6516) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human/Mouse STING/TMEM173 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6516) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STING/TMEM173 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for STING/TMEM173 at approximately 41 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. Sheep Anti-Human STING/TMEM173 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6516) was used at 10 µg/mL followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

STING-mediated effects on vascular remodeling are due to NETs. i, j Confocal images of CD31-positive microvessels (i) and quantification of microvascular density (j) in the peri-infarct cortex at 14 days in mice treated with control IgG or IFNAR-neutralizing antibody, and STING shRNA or control adenovirus (n = 6), unpaired two-tailed Student’s t-test was applied with *P < 0.0001 (Vehicle vs. IFNAR), *P = 0.0007 (Ad-con vs. Ad-sh-STING). Bar = 40 μm. k, l In-vivo multiphoton microscopic images of perfused cortical capillaries with intravenously injected FITC-dextran (k) and quantification of perfused capillary length (l) at 14 days after stroke (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0006 (Vehicle vs. IFNAR), *P = 0.0080 (Ad-con vs. Ad-sh-STING). Bar = 100 μm. m, n In-vivo multiphoton microscopic images (m) of intravenously injected FITC-dextran leakage in cortical vessels at 14 days and quantification of the permeability (P) product of FITC-dextran (n) for each group (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0028 (Vehicle vs. IFNAR), *P = 0.0053 (Ad-con vs. Ad-sh-STING). Bar = 100 μm. Data are presented as mean ± SD. Source data underlying graph a–h, j, l, and n are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

STING-mediated effects on vascular remodeling are due to NETs. f Immunoblot analysis of STING, pTBK1, and pIRF3 in the ischemic cortex at day 3. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

STING-mediated effects on vascular remodeling are due to NETs. b Immunoblot analysis of STING, phosphorylated TBK1 (pTBK1), and pIRF3 in the cortex of mice without stroke or at day 3 after stroke. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

STING-mediated effects on vascular remodeling are due to NETs. d Immunoblot analysis of STING, pTBK1, and pIRF3 in the ischemic cortex at day 3. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

STING-mediated effects on vascular remodeling are due to NETs.h Immunoblot analysis of STING, pTBK1, and pIRF3 in isolated neutrophils for each group (n = 5). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

STING-mediated effects on vascular remodeling are due to NETs. k, l In-vivo multiphoton microscopic images of perfused cortical capillaries with intravenously injected FITC-dextran (k) and quantification of perfused capillary length (l) at 14 days after stroke (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0006 (Vehicle vs. IFNAR), *P = 0.0080 (Ad-con vs. Ad-sh-STING). Bar = 100 μm. m, n In-vivo multiphoton microscopic images (m) of intravenously injected FITC-dextran leakage in cortical vessels at 14 days and quantification of the permeability (P) product of FITC-dextran (n) for each group (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0028 (Vehicle vs. IFNAR), *P = 0.0053 (Ad-con vs. Ad-sh-STING). Bar = 100 μm. Data are presented as mean ± SD. Source data underlying graphs are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

STING-mediated effects on vascular remodeling are due to NETs. m, n In-vivo multiphoton microscopic images (m) of intravenously injected FITC-dextran leakage in cortical vessels at 14 days and quantification of the permeability (P) product of FITC-dextran (n) for each group (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0028 (Vehicle vs. IFNAR), *P = 0.0053 (Ad-con vs. Ad-sh-STING). Bar = 100 μm. Data are presented as mean ± SD. Source data underlying graphs are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6516U-100UG
Alexa Fluor 405	AF6516V-100UG
Alexa Fluor 488	AF6516G-100UG
Alexa Fluor 532	AF6516X-100UG
Alexa Fluor 594	AF6516T-100UG
Alexa Fluor 647	AF6516R-100UG
Alexa Fluor 700	AF6516N-100UG
Alexa Fluor 750	AF6516S-100UG
Alexa Fluor Plus 405	AF6516AFP405-100UG
Alexa Fluor Plus 488	AF6516AFP488-100UG
Alexa Fluor Plus 555	AF6516AFP555-100UG
Alexa Fluor Plus 594	AF6516AFP594-100UG
Alexa Fluor Plus 647	AF6516AFP647-100UG
Alexa Fluor Plus 680	AF6516AFP680-100UG
Allophycocyanin	AF6516A
Biotin	AF6516B
CoraFluor 1	AF6516CL1
DyLight 350	AF6516D
DyLight 405	AF6516E
DyLight 488	AF6516K
DyLight 550	AF6516L
DyLight 594	AF6516M
DyLight 650	AF6516W
DyLight 680	AF6516Y
DyLight 755	AF6516Z
FITC	AF6516F
HRP	AF6516H
Janelia Fluor 525	AF6516JF525
Janelia Fluor 549	AF6516I
Janelia Fluor 646	AF6516J
Janelia Fluor 669	AF6516JF669
PE	AF6516P
PerCP	AF6516C
mFluor Violet 450 SE	AF6516MFV450
mFluor Violet 500 SE	AF6516MFV500
mFluor Violet 610 SE	AF6516MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6516-SP

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NBP2-48684

STING/TMEM173 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP2-48684

<b>Orthogonal Strategies Validation. </b>Analysis in human fallopian tube and liver tissues. Corresponding RNA-seq data are presented for the same tissues.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human lymph node shows strong membranous positivity in follicular dendritic cells.

Staining of human prostate shows moderate membranous positivity in basal layer glandular cells.

Staining of human Fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.

<b>Orthogonal Strategies Validation. </b>Staining of human fallopian tube, liver, lymph node and prostate HPA038534 (A) shows similar protein distribution across tissues to independent antibody HPA038116 (B).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-45998

STING/TMEM173 Antibody (OTI4H1)

0.1 ml

NBP2-45998

Western Blot: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of Human pancreas tissue using STING/TMEM173 Antibody (OTI4H1). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of Carcinoma of Human bladder tissue using STING/TMEM173 Antibody (OTI4H1). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of Human thyroid tissue using STING/TMEM173 Antibody (OTI4H1). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of Carcinoma of Human thyroid tissue using STING/TMEM173 Antibody (OTI4H1). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of Adenocarcinoma of Human ovary tissue using STING/TMEM173 Antibody (OTI4H1). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of Human prostate tissue using STING/TMEM173 Antibody (OTI4H1). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of Carcinoma of Human pancreas tissue using STING/TMEM173 Antibody (OTI4H1). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of Carcinoma of Human lung tissue using STING/TMEM173 Antibody (OTI4H1). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of Adenocarcinoma of Human colon tissue STING/TMEM173 Antibody (OTI4H1). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: STING/TMEM173 Antibody (OTI4H1) [NBP2-45998] - Analysis of Carcinoma of Human prostate tissue using STING/TMEM173 Antibody (OTI4H1). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-74402AF350
Alexa Fluor 405	NBP2-74402AF405
Alexa Fluor 488	NBP2-74402AF488
Alexa Fluor 532	NBP2-74402AF532
Alexa Fluor 594	NBP2-74402AF594
Alexa Fluor 647	NBP2-74402AF647
Alexa Fluor 700	NBP2-74402AF700
Alexa Fluor 750	NBP2-74402AF750
Alexa Fluor Plus 405	NBP2-74402AFP405
Alexa Fluor Plus 488	NBP2-74402AFP488
Alexa Fluor Plus 555	NBP2-74402AFP555
Alexa Fluor Plus 594	NBP2-74402AFP594
Alexa Fluor Plus 647	NBP2-74402AFP647
Alexa Fluor Plus 680	NBP2-74402AFP680
Allophycocyanin	NBP2-74402APC
Biotin	NBP2-74402B
CoraFluor 1	NBP2-74402CL1
DyLight 350	NBP2-74402UV
DyLight 405	NBP2-74402V
DyLight 488	NBP2-74402G
DyLight 550	NBP2-74402R
DyLight 594	NBP2-74402DL594
DyLight 650	NBP2-74402C
DyLight 680	NBP2-74402FR
DyLight 755	NBP2-74402IR
FITC	NBP2-74402F
HRP	NBP2-74402H
Janelia Fluor 525	NBP2-74402JF525
Janelia Fluor 549	NBP2-74402JF549
Janelia Fluor 646	NBP2-74402JF646
Janelia Fluor 669	NBP2-74402JF669
PE	NBP2-74402PE
PerCP	NBP2-74402PCP
mFluor Violet 450 SE	NBP2-74402MFV450
mFluor Violet 500 SE	NBP2-74402MFV500
mFluor Violet 610 SE	NBP2-74402MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-74402

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NBP2-38389

STING/TMEM173 Antibody - BSA Free

0.1 ml

NBP2-38389

<b>Orthogonal Strategies Validation. </b>Staining of human fallopian tube, liver, lymph node and prostate HPA038116 (A) shows similar protein distribution across tissues to independent antibody HPA038534 (B).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-38389-25ul

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NBP3-20616

STING/TMEM173 Antibody (STING1/8129R)

100 μg

NBP3-20616

Formalin-fixed, paraffin-embedded human serous ovarian carcinoma stained with STING/TMEM173 antibody (STING1/8129R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with STING/TMEM173 antibody (STING1/8129R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human mammary cancer stained with STING/TMEM173 antibody (STING1/8129R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with STING/TMEM173 antibody (STING1/8129R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human spleen stained with STING/TMEM173 antibody (STING1/8129R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with STING/TMEM173 antibody (STING1/8129R). Inset: PBS instead of primary antibody; secondary only negative control.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-20698AF350
Alexa Fluor 405	NBP3-20698AF405
Alexa Fluor 488	NBP3-20698AF488
Alexa Fluor 532	NBP3-20698AF532
Alexa Fluor 594	NBP3-20698AF594
Alexa Fluor 647	NBP3-20698AF647
Alexa Fluor 700	NBP3-20698AF700
Alexa Fluor 750	NBP3-20698AF750
Alexa Fluor Plus 405	NBP3-20698AFP405
Alexa Fluor Plus 488	NBP3-20698AFP488
Alexa Fluor Plus 555	NBP3-20698AFP555
Alexa Fluor Plus 594	NBP3-20698AFP594
Alexa Fluor Plus 647	NBP3-20698AFP647
Alexa Fluor Plus 680	NBP3-20698AFP680
Allophycocyanin	NBP3-20698APC
Biotin	NBP3-20698B
CoraFluor 1	NBP3-20698CL1
DyLight 350	NBP3-20698UV
DyLight 405	NBP3-20698V
DyLight 488	NBP3-20698G
DyLight 550	NBP3-20698R
DyLight 594	NBP3-20698DL594
DyLight 650	NBP3-20698C
DyLight 680	NBP3-20698FR
DyLight 755	NBP3-20698IR
FITC	NBP3-20698F
HRP	NBP3-20698H
Janelia Fluor 525	NBP3-20698JF525
Janelia Fluor 549	NBP3-20698JF549
Janelia Fluor 646	NBP3-20698JF646
Janelia Fluor 669	NBP3-20698JF669
PE	NBP3-20698PE
PerCP	NBP3-20698PCP
mFluor Violet 450 SE	NBP3-20698MFV450
mFluor Violet 500 SE	NBP3-20698MFV500
mFluor Violet 610 SE	NBP3-20698MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-20698

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NBP3-19785

STING/TMEM173 Antibody (S06-9A7)

100 ul

NBP3-19785

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-13941

STING/TMEM173 Antibody (STING1/7431)

100 μg

NBP3-13941

Western blot analysis of HDLM-2 cell lysate using STING/TMEM173 antibody (STING1/7431).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-14076AF350
Alexa Fluor 405	NBP3-14076AF405
Alexa Fluor 488	NBP3-14076AF488
Alexa Fluor 532	NBP3-14076AF532
Alexa Fluor 594	NBP3-14076AF594
Alexa Fluor 647	NBP3-14076AF647
Alexa Fluor 700	NBP3-14076AF700
Alexa Fluor 750	NBP3-14076AF750
Alexa Fluor Plus 405	NBP3-14076AFP405
Alexa Fluor Plus 488	NBP3-14076AFP488
Alexa Fluor Plus 555	NBP3-14076AFP555
Alexa Fluor Plus 594	NBP3-14076AFP594
Alexa Fluor Plus 647	NBP3-14076AFP647
Alexa Fluor Plus 680	NBP3-14076AFP680
Allophycocyanin	NBP3-14076APC
Biotin	NBP3-14076B
CoraFluor 1	NBP3-14076CL1
DyLight 350	NBP3-14076UV
DyLight 405	NBP3-14076V
DyLight 488	NBP3-14076G
DyLight 550	NBP3-14076R
DyLight 594	NBP3-14076DL594
DyLight 650	NBP3-14076C
DyLight 680	NBP3-14076FR
DyLight 755	NBP3-14076IR
FITC	NBP3-14076F
HRP	NBP3-14076H
Janelia Fluor 525	NBP3-14076JF525
Janelia Fluor 549	NBP3-14076JF549
Janelia Fluor 646	NBP3-14076JF646
Janelia Fluor 669	NBP3-14076JF669
PE	NBP3-14076PE
PerCP	NBP3-14076PCP
mFluor Violet 450 SE	NBP3-14076MFV450
mFluor Violet 500 SE	NBP3-14076MFV500
mFluor Violet 610 SE	NBP3-14076MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-14076

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NBP3-50953

STING/TMEM173 Antibody (24GB5890)

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-20617

STING/TMEM173 Antibody (STING1/8187R)

100 μg

NBP3-20617

Formalin-fixed, paraffin-embedded human spleen stained with STING/TMEM173 antibody (STING1/8187R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human serous ovarian carcinoma stained with STING/TMEM173 antibody (STING1/8187R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-20699AF350
Alexa Fluor 405	NBP3-20699AF405
Alexa Fluor 488	NBP3-20699AF488
Alexa Fluor 532	NBP3-20699AF532
Alexa Fluor 594	NBP3-20699AF594
Alexa Fluor 647	NBP3-20699AF647
Alexa Fluor 700	NBP3-20699AF700
Alexa Fluor 750	NBP3-20699AF750
Alexa Fluor Plus 405	NBP3-20699AFP405
Alexa Fluor Plus 488	NBP3-20699AFP488
Alexa Fluor Plus 555	NBP3-20699AFP555
Alexa Fluor Plus 594	NBP3-20699AFP594
Alexa Fluor Plus 647	NBP3-20699AFP647
Alexa Fluor Plus 680	NBP3-20699AFP680
Allophycocyanin	NBP3-20699APC
Biotin	NBP3-20699B
CoraFluor 1	NBP3-20699CL1
DyLight 350	NBP3-20699UV
DyLight 405	NBP3-20699V
DyLight 488	NBP3-20699G
DyLight 550	NBP3-20699R
DyLight 594	NBP3-20699DL594
DyLight 650	NBP3-20699C
DyLight 680	NBP3-20699FR
DyLight 755	NBP3-20699IR
FITC	NBP3-20699F
HRP	NBP3-20699H
Janelia Fluor 525	NBP3-20699JF525
Janelia Fluor 549	NBP3-20699JF549
Janelia Fluor 646	NBP3-20699JF646
Janelia Fluor 669	NBP3-20699JF669
PE	NBP3-20699PE
PerCP	NBP3-20699PCP
mFluor Violet 450 SE	NBP3-20699MFV450
mFluor Violet 500 SE	NBP3-20699MFV500
mFluor Violet 610 SE	NBP3-20699MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-20699

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

STING/TMEM173 Antibodies

STING/TMEM173 Antibody - BSA Free

STING/TMEM173 Antibody (723505) [Unconjugated]

STING/TMEM173 Antibody (2922D) - BSA Free

STING/TMEM173 Antibody [Unconjugated]

STING/TMEM173 Antibody - BSA Free

STING/TMEM173 Antibody (OTI4H1)

STING/TMEM173 Antibody - BSA Free

STING/TMEM173 Antibody (STING1/8129R)

STING/TMEM173 Antibody (S06-9A7)

STING/TMEM173 Antibody (STING1/7431)

STING/TMEM173 Antibody (24GB5890)

STING/TMEM173 Antibody (STING1/8187R)