TBK1 Antibody (108A429)

Images

 
Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis using Azide and BSA Free version of NB100-56705. Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 ...read more
Immunocytochemistry/ Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Immunohistochemistry: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE)) ...read more
Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis of Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells ...read more
Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Analysis of FFPE human testis tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:200. The staining was developed with ...read more
Simple Western: TBK1 Antibody (108A429) [NB100-56705] - Lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clone
108A429
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TBK1 Antibody (108A429) Summary

Immunogen
A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).
Predicted Species
Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TBK1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Simple Western 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
In Western Blot this antibody detects a band at 80 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-56705 in the following applications:

Publications
Read Publications using
NB100-56705 in the following applications:

  • IP
    1 publication
  • WB
    18 publications

Reactivity Notes

Predicted to react with Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TBK1 Antibody (108A429)

  • EC 2.7.11
  • EC 2.7.11.1
  • FLJ11330
  • FTDALS4
  • NAK Antibody
  • NAK serine antibody
  • NAK serine/threonine-protein kinase TBK 1
  • NAK
  • NAKserine/threonine-protein kinase TBK1
  • NF-kappa-B-activating kinase
  • NFKB-Activating Kinase
  • T2K
  • TANK antibody
  • TANK binding kinase antibody
  • TANK-binding kinase 1NF-kB-activating kinase
  • TBK 1
  • TBK1

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recently, a new IKK family member, TBK1 [(TANK-binding kinase), also known as NAK and T2K] has been identified. TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TBK1 Antibody (NB100-56705)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(18)
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Human
(11)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB100-56705 Applications Species
Deng M, Tam JW, Wang L et al. TRAF3IP3 negatively regulates cytosolic RNA induced anti-viral signaling by promoting TBK1 K48 ubiquitination Nat Commun May 4 2020 [PMID: 32366851] (IP, Rat) IP Rat
Shi D, Chen M, Liu L et al. Anti-influenza A virus mechanism of three representative compounds from Flos Trollii via TLRs signaling pathways J Ethnopharmacol Jan 28 2020 [PMID: 32004628] (WB, Mouse) WB Mouse
van Tol S, Atkins C, Bharaj P et al. VAMP8 Contributes to the TRIM6-Mediated Type I Interferon Antiviral Response during West Nile Virus Infection J. Virol. Jan 6 2020 [PMID: 31694946] (WB) WB
Wuerth JD, Habjan M, Wulle J et al. NSs protein of Sandfly fever Sicilian phlebovirus counteracts interferon induction by masking the DNA-binding domain of interferon regulatory factor 3. J. Virol. Sep 19 2018 [PMID: 30232186] (WB, Human) WB Human
Gulen MF, Koch U, Haag SM et al. Signalling strength determines proapoptotic functions of STING Nat Commun 2017 Sep 05 [PMID: 28874664] (WB) WB
Kwon D, Park E, Sesaki H, Kang SJ et al. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) suppresses STING-mediated DNA sensing pathway through inducing mitochondrial fission Biochem. Biophys. Res. Commun. 2017 Aug 30 [PMID: 28859978] (WB) WB
Slowicka K, Vereecke L, McGuire C et al. Optineurin deficiency in mice is associated with increased sensitivity to Salmonella but does not affect proinflammatory NF-kB signaling. Eur. J. Immunol. 2015 Dec 17 [PMID: 26677802] (WB, Mouse)

Details:
TBK1 antibody was used for WB analysis of bone marrow-derived macrophages ( from OPTN +/+ and ?/? mice) after stimulation with 10 ug/mL poly(I,C) or 100 ng/mL LPS (Figure 3A).
WB Mouse
Mukawera E, Chartier S, Williams V, Pagano PJ. Redox-modulating agents target NOX2-dependent IKKEpsilon oncogenic kinase expression and proliferation in human breast cancer cell lines Redox Biology. 2015 Jun 23 [PMID: 26177467] (WB, Human)

Details:
NAK/TBK1 antibody was used in WB application for the detection of Tank Binding Kinase 1 protein on lysates from human breast cancer cell lines MCF-7 and ZR75.1 (Fig. 1B). WB was also performed on MCF-7 and ZR75.1 cells that we left untreated or treated with Tempol (T), Gentian Violet (GV) or Brilliant Green (BG) at different concentrations for 24 h (Fig. 3A and 3D), and on MCF-7 cells treated control-RNAi, NOX2-RNAi, NOX5RNAi, IKK epsilon RNAi (Fig. 7C).
WB Human
Yu HB, Croxen MA, Marchiando AM et al. Autophagy Facilitates Salmonella Replication in HeLa Cells. MBio 2014 Mar 12 [PMID: 24618251] (WB, Human)

Details:
HeLa, Fig 3. The specificity of the NAk(TBk1) mAb was validated in NAk(TBk1)-siRNA treated HeLa cells by WB, Fig 3.
WB Human
Conlon J, Burdette DL, Sharma S et al. Mouse, but not human STING, binds and signals in response to the vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid. J Immunol. 2013 May 15 [PMID: 23585680]
Show All 26 Publications.

Review for TBK1 Antibody (NB100-56705) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56705:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot TBK1 NB100-56705
Enlarge
5
reviewed by:
Anonymous
WB Human 12/21/2017
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Summary

ApplicationWestern Blot
Sample Tested293 whole cell lysate
SpeciesHuman
LotAB013008A-03

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TBK1 Antibody (NB100-56705). (Showing 1 - 7 of 7 FAQs).

  1. What is the exact sequence this TBK1 antibody recognizes?
    • The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
  2. I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
    • While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
  3. We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
    • Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
  4. Is there a smaller size of your TBK1 antibody?
    • This TBK1 antibody is offered in a smaller 0.025 mg size.
  5. Are there any publications where this TBK1 antibody was used in western blot for mouse?
    • This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
  6. Cant this TBK1 antibody be conjugated to PE for FLOW?
    • Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
  7. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
    • These antibodies are all purified from tissue culture supernatant.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56705AF350
Alexa Fluor 405 NB100-56705AF405
Alexa Fluor 488 NB100-56705AF488
Alexa Fluor 532 NB100-56705AF532
Alexa Fluor 594 NB100-56705AF594
Alexa Fluor 647 NB100-56705AF647
Alexa Fluor 700 NB100-56705AF700
Alexa Fluor 750 NB100-56705AF750
Biotin NB100-56705B
DyLight 350 NB100-56705UV
DyLight 405 NB100-56705V
DyLight 488 NB100-56705G
DyLight 550 NB100-56705R
DyLight 594 NB100-56705DL594
DyLight 650 NB100-56705C
DyLight 680 NB100-56705FR
DyLight 755 NB100-56705IR
HRP NB100-56705H
Janelia Fluor 549 NB100-56705JF549
Janelia Fluor 646 NB100-56705JF646

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Blogs on TBK1.

Autophagy Research Update: What a difference a year makes!
By Christina Towers, PhD Over the last two decades the field of autophagy has exploded! Innovative techniques, comprehensive analysis and disease-relevant models have yielded basic and clinical discoveries of conseque...  Read full blog post.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
12/21/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol TBK1
Entrez
Uniprot