TBK1 Antibody (108A429)


Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Simple Western lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing ...read more
Immunohistochemistry: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A dilution ...read more
Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis of TBK1 using TBK1 antibody. Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ...read more
Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis of Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Human testis (Formalin-Fixed Paraffin-Embedded (FFPE).

Product Details

Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
0.5 mg/ml

TBK1 Antibody (108A429) Summary

A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified


  • Western Blot 1-2 ug/ml
  • Simple Western 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
In Western Blot this antibody detects a band at 80 kDa.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Theoretical MW
84 kDa
Read Publications using
NB100-56705 in the following applications:

  • WB
    13 publications

Reactivity Notes

Predicted to react with Chimpanzee.

Alternate Names for TBK1 Antibody (108A429)

  • EC 2.7.11
  • EC
  • FLJ11330
  • NAK Antibody
  • NAK serine antibody
  • NAK serine/threonine-protein kinase TBK 1
  • NAKserine/threonine-protein kinase TBK1
  • NF-kappa-B-activating kinase
  • T2K
  • TANK antibody
  • TANK binding kinase antibody
  • TANK-binding kinase 1NF-kB-activating kinase
  • TBK 1


NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recently, a new IKK family member, TBK1 [(TANK-binding kinase), also known as NAK and T2K] has been identified. TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TBK1 Antibody (NB100-56705)(20)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB100-56705 Applications Species
Slowicka K, Vereecke L, McGuire C et al. Optineurin deficiency in mice is associated with increased sensitivity to Salmonella but does not affect proinflammatory NF-kB signaling. Eur. J. Immunol. 2015 Dec 17 [PMID:26677802] (WB, Mouse) WB Mouse
Mukawera E, Chartier S, Williams V, Pagano PJ. Redox-modulating agents target NOX2-dependent IKKEpsilon oncogenic kinase expression and proliferation in human breast cancer cell lines Redox Biology. 2015 Jun 23 [PMID:26177467] (WB, Human) WB Human
Yu HB, Croxen MA, Marchiando AM et al. Autophagy Facilitates Salmonella Replication in HeLa Cells. MBio 2014 Mar 12 [PMID:24618251] (WB, Human) WB Human
Conlon J, Burdette DL, Sharma S et al. Mouse, but not human STING, binds and signals in response to the vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid. J Immunol. 2013 May 15 [PMID:23585680]
Tamassia N, Bazzoni F, Le Moigne V et al. IFN-B expression is directly activated in human neutrophils transfected with plasmid DNA and is further increased via TLR-4-mediated signaling. J Immunol. 2012 Aug 1 [PMID:22730532] (WB) WB
Delhase M, Kim SY, Lee H et al. TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. Proc Natl Acad Sci U S A. 2012 Jan 24 [PMID:22203995]
Ogasawara N, Sasaki M, Itoh Y et al. Rebamipide suppresses TLR-TBk1 signaling pathway resulting in regulating IRF3/7 and IFN-alpha/beta reduction. J Clin Biochem Nutr. [PMID:21373269] (WB, Human) WB Human
Joung SM, Park ZY, Rani S et al. Akt contributes to activation of the TRIF-dependent signaling pathways of TLRs by interacting with TANK-binding kinase 1. J Immunol. 2011 Jan 1 [PMID:21106850] (WB) WB
Marchlik E, Thakker P, Carlson T et al. Mice lacking Tbk1 activity exhibit immune cell infiltrates in multiple tissues and increased susceptibility to LPS-induced lethality. J Leukoc Biol. 2010 Dec [PMID:20651301] (WB, Mouse) WB Mouse
Berthet J, Damien P, Hamzeh-Cognasse H et al. Toll-like receptor 4 signal transduction in platelets: novel pathways. Br J Haematol. [PMID:20618335] (WB, Human) WB Human
Show All 20 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TBK1 Antibody (NB100-56705). (Showing 1 - 7 of 7 FAQs).

  1. What is the exact sequence this TBK1 antibody recognizes?
    • The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
  2. I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
    • While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
  3. We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
    • Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
  4. Is there a smaller size of your TBK1 antibody?
    • This TBK1 antibody is offered in a smaller 0.025 mg size.
  5. Are there any publications where this TBK1 antibody was used in western blot for mouse?
    • This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
  6. Cant this TBK1 antibody be conjugated to PE for FLOW?
    • Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
  7. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
    • These antibodies are all purified from tissue culture supernatant.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-56705AF405
Alexa Fluor (R) 488 Labeled NB100-56705AF488
Alexa Fluor (R) 647 Labeled NB100-56705AF647
Alexa Fluor (R) 700 Labeled NB100-56705AF700
Allophycocyanin Labeled NB100-56705APC
Biotin Labeled NB100-56705B
DyLight 350 Labeled NB100-56705UV
DyLight 405 Labeled NB100-56705V
DyLight 405LS Labeled NB100-56705V3
DyLight 488 Labeled NB100-56705G
DyLight 550 Labeled NB100-56705R
DyLight 650 Labeled NB100-56705C
DyLight 680 Labeled NB100-56705FR
DyLight 755 Labeled NB100-56705IR
HRP Labeled NB100-56705H
PE Labeled NB100-56705PE
PerCP Labeled NB100-56705PCP

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STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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Product PDFs


Gene Symbol TBK1

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