MUL1 Antibody (5H6.2D5)

Western Blot: MUL1 Antibody (5H6.2D5) [NBP2-31361] - WB detection of partial recombinant protein MUL1 by using MUL1 antibody (clone 5H6.2D5) at a concentration of 0.01 ug/ml.

Immunohistochemistry-Paraffin: MUL1 Antibody (5H6.2D5) [NBP2-31361] - IHC-P analysis of MUL1 protein in a transverse section of normal skeletal muscle from human using 5 ug/ml concentration of MUL1 antibody (clone 5H6.2D5). The myocytes and blood capillaries depicted strong staining whereas the nuclei were found negative for MUL1.

Immunohistochemistry-Paraffin: MUL1 Antibody (5H6.2D5) [NBP2-31361] - IHC-P analysis of MUL1 protein in a section of normal prostate from human using 5 ug/ml concentration of MUL1 antibody (clone 5H6.2D5). Strong cytoplasmic staining was observed in the epithelial columnar and basal cells of prostate glands.

Immunohistochemistry-Paraffin: MUL1 Antibody (5H6.2D5) [NBP2-31361] - IHC-P analysis of MUL1 protein in a section of normal cardiac stomach from human using 5 ug/ml concentration of MUL1 antibody (clone 5H6.2D5). The cardiac glands showed distinct cytoplasmic staining, wherein, the parietal cells as well as the chief cells were found positive for MUL1.

• Reactivity: Hu, Pm
• Applications: WB, IHC, IHC-P
• Clone: 5H6.2D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

MUL1 Antibody (5H6.2D5) Summary

• Immunogen: Partial recombinant protein made to a C-terminal portion of the human MUL1 protein (between residues 200-352) [UniProt Q969V5]
• Localization: Mitochondria, Peroxisomes
• Predicted Species: Primate (99%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUL1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot
• Application Notes: MUL1 (mitochondrial ubiquitin ligase activator of NFKB 1) is a 352 amino acids long protein (predicted molecular weight 39.8 kDa) which localizes to the mitochondrion's outer membrane as a multi-pass membrane protein and from there, it may get transported to the peroxisomes via mitochondrion-derived vesicles. In immunostaining experiments, this MUL1 antibody is expected to generate a cytoplasmic staining pattern.

Reactivity Notes

Human. The immunogen sequence shows 99% similarity to Monkey's MUL1 and is 84% similar to isoform 1 and 3 of Mouse's MUL1 .

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for MUL1 Antibody (5H6.2D5)

• C1orf166mitochondrial E3 ubiquitin ligase 1
• E3 ubiquitin-protein ligase MUL1
• EC 6.3.2
• EC 6.3.2.-
• FLJ12875
• GIDERP11-401M16.2
• Growth inhibition and death E3 ligase
• MAPLE3 ubiquitin ligase
• mitochondrial E3 ubiquitin protein ligase 1
• mitochondrial ubiquitin ligase activator of NFKB 1
• mitochondrial ubiquitin ligase activator of NF-kB
• Mitochondrial-anchored protein ligase
• MULANchromosome 1 open reading frame 166
• Putative NF-kappa-B-activating protein 266
• RING finger protein 218
• RNF218mitochondria-anchored protein ligase

Background

MUL1 is an E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, MUL1 activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MUL1
• Entrez:
  • 79594(Human)