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MUL1 Antibody (5H6.2D5)

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Western Blot: MUL1 Antibody (5H6.2D5) [NBP2-31361] - WB detection of partial recombinant protein MUL1 by using MUL1 antibody (clone 5H6.2D5) at a concentration of 0.01 ug/ml.
Western Blot: MUL1 Antibody (5H6.2D5) [NBP2-31361] - WB detection of partial recombinant protein MUL1 by using MUL1 antibody (clone 5H6.2D5) at a concentration of 0.01 ug/ml.
Immunohistochemistry-Paraffin: MUL1 Antibody (5H6.2D5) [NBP2-31361] - IHC-P analysis of MUL1 protein in a transverse section of normal skeletal muscle from human using 5 ug/ml concentration of MUL1 antibody (clone ...read more
Immunohistochemistry-Paraffin: MUL1 Antibody (5H6.2D5) [NBP2-31361] - IHC-P analysis of MUL1 protein in a section of normal prostate from human using 5 ug/ml concentration of MUL1 antibody (clone 5H6.2D5). Strong ...read more
Immunohistochemistry-Paraffin: MUL1 Antibody (5H6.2D5) [NBP2-31361] - IHC-P analysis of MUL1 protein in a section of normal cardiac stomach from human using 5 ug/ml concentration of MUL1 antibody (clone 5H6.2D5). The ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clone
5H6.2D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-31361AF350
Alexa Fluor 405
NBP2-31361AF405
Alexa Fluor 488
NBP2-31361AF488
Alexa Fluor 532
NBP2-31361AF532
Alexa Fluor 594
NBP2-31361AF594
Alexa Fluor 647
NBP2-31361AF647
Alexa Fluor 700
NBP2-31361AF700
Alexa Fluor 750
NBP2-31361AF750
Biotin
NBP2-31361B
DyLight 350
NBP2-31361UV
DyLight 405
NBP2-31361V
DyLight 488
NBP2-31361G
DyLight 550
NBP2-31361R
DyLight 594
NBP2-31361DL594
DyLight 650
NBP2-31361C
DyLight 680
NBP2-31361FR
DyLight 755
NBP2-31361IR
FITC
NBP2-31361F
HRP
NBP2-31361H
Janelia Fluor 549
NBP2-31361JF549
Janelia Fluor 646
NBP2-31361JF646
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MUL1 Antibody (5H6.2D5) Summary

Immunogen
Partial recombinant protein made to a C-terminal portion of the human MUL1 protein (between residues 200-352) [UniProt Q969V5]
Localization
Mitochondria, Peroxisomes
Predicted Species
Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUL1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
MUL1 (mitochondrial ubiquitin ligase activator of NFKB 1) is a 352 amino acids long protein (predicted molecular weight 39.8 kDa) which localizes to the mitochondrion's outer membrane as a multi-pass membrane protein and from there, it may get transported to the peroxisomes via mitochondrion-derived vesicles. In immunostaining experiments, this MUL1 antibody is expected to generate a cytoplasmic staining pattern.

Reactivity Notes

Human. The immunogen sequence shows 99% similarity to Monkey's MUL1 and is 84% similar to isoform 1 and 3 of Mouse's MUL1.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MUL1 Antibody (5H6.2D5)

  • C1orf166mitochondrial E3 ubiquitin ligase 1
  • E3 ubiquitin-protein ligase MUL1
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ12875
  • GIDERP11-401M16.2
  • Growth inhibition and death E3 ligase
  • MAPLE3 ubiquitin ligase
  • mitochondrial E3 ubiquitin protein ligase 1
  • mitochondrial ubiquitin ligase activator of NFKB 1
  • mitochondrial ubiquitin ligase activator of NF-kB
  • Mitochondrial-anchored protein ligase
  • MULANchromosome 1 open reading frame 166
  • Putative NF-kappa-B-activating protein 266
  • RING finger protein 218
  • RNF218mitochondria-anchored protein ligase

Background

MUL1 is an E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, MUL1 activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MUL1 Antibody (5H6.2D5)
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Applications: WB, IHC, IHC-P

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Product General Protocols

View specific protocols for MUL1 Antibody (NBP2-31361): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-31361AF350
Alexa Fluor 405 NBP2-31361AF405
Alexa Fluor 488 NBP2-31361AF488
Alexa Fluor 532 NBP2-31361AF532
Alexa Fluor 594 NBP2-31361AF594
Alexa Fluor 647 NBP2-31361AF647
Alexa Fluor 700 NBP2-31361AF700
Alexa Fluor 750 NBP2-31361AF750
Biotin NBP2-31361B
DyLight 350 NBP2-31361UV
DyLight 405 NBP2-31361V
DyLight 488 NBP2-31361G
DyLight 550 NBP2-31361R
DyLight 594 NBP2-31361DL594
DyLight 650 NBP2-31361C
DyLight 680 NBP2-31361FR
DyLight 755 NBP2-31361IR
FITC NBP2-31361F
HRP NBP2-31361H
Janelia Fluor 549 NBP2-31361JF549
Janelia Fluor 646 NBP2-31361JF646
Learn about Custom Antibody Labeling

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MUL1 - A Mito's best friend
MUL1 is an E3 ubiquitin-protein ligase with a RING finger domain that controls mitochondrial morphology, fragmentation and localization. E3 ubiquitin ligases accept the component ubiquitin from a donor E2 ubiquitin-conjugating directly transfer this u...  Read full blog post.

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Bioinformatics

Gene Symbol MUL1
Entrez